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Multiplex FISH

by admin
All Applications, Cell-Based Analysis, Fluorescent, Multiplexing, Oncology, RNAscope
June 23, 2015

MULTIPLEX FISH

(Designed for Fluorescent RNAscope®)

 

Product Overview

Indica Labs’ Multiplex FISH module quantifies an unlimited number of DNA or RNA FISH probes on a cell-by-cell basis. This analysis module can be used with most multiplex RNA FISH assays including RNAscope® and supports the H-score protocol for RNAscope® recommended by the manufacturer, ACD Bio.

Watch a webinar from two of our customers discussing how they use and analyze RNAscope assays in their research and download the full ACD Bio™ RNAscope Data Analysis Guide by clicking the links here.

In the example above the module measures a two-plex RNAscope assay on pelleted cancer cells. Single cells are identified using the nuclear dye DAPI. The multiplexed red and green probes are measured within the cell boundaries (spots per cell and probe area per cell). The module reports the average number of each probe per cell or across the entire region of analysis. In the analysis markup image, cells positive for the red probe alone are highlighted in different shades of red, cells positive for the green probe alone in different shades of green and cells positive for both probes are highlighted in shades of yellow. The color shading is determined by the number of probes and the corresponding RNAscope H-score, dark color = lower expression and light color = higher expression.

The Multiplex FISH module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

MULTIPLEX FISH

(Designed for RNAscope®)

Indica Labs’ Multiplex FISH module quantifies an unlimited number of DNA or RNA FISH probes on a cell-by-cell basis. This analysis module can be used with most multiplex RNA FISH assays including RNAscope® and supports the H-score protocol for RNAscope® recommended by the manufacturer, Advanced Cell Diagnostics.

Watch a webinar from two of our customers discussing how they use and analyze RNAscope assays in their research and download the full ACD Bio™ RNAscope Data Analysis Guide by clicking the links here.

In the example above the module measures a two-plex RNAscope assay on pelleted cancer cells. Single cells are identified using the nuclear dye DAPI. The multiplexed red and green probes are measured within the cell boundaries (spots per cell and probe area per cell). The module reports the average number of each probe per cell or across the entire region of analysis. In the analysis markup image, cells positive for the red probe alone are highlighted in different shades of red, cells positive for the green probe alone in different shades of green and cells positive for both probes are highlighted in shades of yellow. The color shading is determined by the number of probes and the corresponding RNAscope H-score, dark color = lower expression and light color = higher expression.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Here are a few publications that cite use of our ISH and FISH modules.  Your publication not on the list?  Drop us an email to let us know about it!

Title Author Year Journal
Age-determined expression of priming protease TMPRSS2 and localization of SARS-CoV-2 infection in the lung epithelium Schuler BA, Habermann CA, Plosa EJ, Taylor CJ, Jetter C, Kapp ME, Benjamin JT, Gulleman P,
Nichols DS, Lior Z. Braunstein, Hackett A, Koval A, Guttentag SH, Blackwell TS, Vanderbilt COVID-19 Consortium Cohort10, Webber SA,
Banovich NE, Kropski JA, Sucre JMS, HCA Lung Biological Network		 2020 bioRxiv
Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Meyer MA, Baer JM, Knolhoff BL, Nywening TM, Panni RZ, Su X, Weilbaecher KN, Hawkins WG, Ma C, Fields RC, Linehan DC, Challen GA, Faccio R, Aft RL, DeNardo DG 2018 Nature Communications
A randomized, open-label study of the efficacy and safety of AZD4547 monotherapy versus paclitaxel for the treatment of advanced gastric adenocarcinoma with FGFR2 polysomy or gene amplification Van Cutsem E, Bang Y-J,  Mansoor W,  Petty RD, Chao Y, Cunningham D, Ferry DR, N. R. Smith NR, Frewer P, Ratnayake J, Stockman PK, Kilgour E, and Landers D 2017 Annals of Oncology
APNG as a prognostic marker in patients with glioblastoma Fosmark S, Hellwege S, Dahlrot RH, Jensen KL, Derand H, Lohse J, Sørensen MD, Hansen S, Kristensen BW 2017 PLOS One
Differential Cytokine Gene Expression in Granulomas from Lungs and Lymph Nodes of Cattle Experimentally Infected with Aerosolized Mycobacterium bovis Palmer MV, Thacker TC, Waters WR 2016 PLOS One
Fully automated RNAscope in situ hybridization assays for formalin‐fixed paraffin‐embedded cells and tissues Anderson CM, Zhang B, Miller M, Butko E, Wu X, Laver T, Kernag C, Kim J, Luo Y, Lamparski H 2016 Journal of Cellular Biochemistry
G-protein-independent coupling of MC4R to Kir7. 1 in hypothalamic neurons Ghamari-Langroudi M, Digby GJ, Sebag JA, Millhauser GL, Palomino R, Matthews R, Gillyard T, Panaro BL, Tough IR, Cox HM, Denton JS, Cone RD 2015 Nature

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.

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