Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

Multiplex IHC



Product Overview

Indica Labs’ Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane) and can be used for a variety of IHC applications.  This module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  Each cell is individually measured for cytoplasmic, nuclear, and/or membrane positivity.  The software also reports a variety of other statistics relating size and staining intensity metrics.

In the example above, a tissue core is stained with a brown (DAB) nuclear marker, blue membrane marker, pink/red cytoplasmic marker, and nuclear hematoxylin counterstain.  Cells are segmented using a combination of the two nuclear stains (hematoxylin and DAB) and one membrane stain (blue).  In the corresponding markup image, positive nuclei are colored brown and negative nuclei colored blue.  Similarly, cells identified as positive for the membrane marker are colored blue and cells identified as positive for cytoplasmic marker are pink.  Advanced staining courtesy of the Trev and Joyce Deeley Research Centre, BC Cancer Agency.

The Multiplex IHC module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and  file formats.

Contact for product demonstration and pricing information or upload some images for a free trial.


Indica Labs’ Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane) and can be used for a variety of IHC applications. This module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue. Each cell is individually measured for cytoplasmic, nuclear, and/or membrane positivity. The software also reports a variety of other statistics relating size and staining intensity metrics.

In the example above, a tissue core is stained with a brown (DAB) nuclear marker, blue membrane marker, pink/red cytoplasmic marker, and nuclear hematoxylin counterstain. Cells are segmented using a combination of the two nuclear stains (hematoxylin and DAB) and one membrane stain (blue). In the corresponding markup image, positive nuclei are colored brown and negative nuclei colored blue. Similarly, cells identified as positive for the membrane marker are colored blue and cells identified as positive for cytoplasmic marker are pink. Advanced staining courtesy of the Trev and Joyce Deeley Research Centre, BC Cancer Agency.

Here are a few publications that cite use of our Multiplex IHC module.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Linking epigenetic signature and metabolic phenotype in IDH mutant and IDH wildtype diffuse gliomaBuran Y, Filipski K, Bernatz S, Baumgarten P, Roller B, Zinke J, Zeiner PS, Ilina E, Senft C, Ronellenfitsch MW, Plate KH, Bähr O, Hattingen E, Steinbach JP, Mittelbron M, Harter PN2020Neuropathology and Applied NeurobiologyNeuroscienceMultiplex IHC, TMA
Neoadjuvant immunotherapy leads to pathological responses in MMR-proficient and MMR-deficient early-stage colon cancersChalabi M, Fanchi LF, Dijkstra KK, Van den Berg JG, Aalbers AG, Sikorska K, Lopez-Yurda M, Grootscholten C, Beets GL, Snaebjornsson P, Maas M, Mertz J, Veninga V, Bounova G, Broeks A, Beets-Tan RG, de Wijkerslooth TR, van Lent AU, Marsman HA, Nuijten E, Kok NF, Kuiper M, Verbeek WH, Kok M, Van Leerdam ME, Schumacher TN, Voest EE, Haanen JB2020Nature MedicineImmuno-oncologyMultiplex IHC, Serial Section
Computational image analysis of T-cell infiltrates in resectable gastric cancer: association with survival and molecular subtypesChalloner BR, von Loga K, Woolston A, Griffiths B, Sivamanoharan N, Semiannikova M, Newey A, Barber LJ, Mansfield D, Hewitt LC, Saito Y, Davarzani N, Starling N, Melcher A, Grabsch HI, Gerlinger M2020JNCI: Journal of the National Cancer InstituteImmuno-oncologyMultiplex IHC
Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary InfectionDesai N, Neyaz A, Szabolcs A, Shih AR, Chen JH, Thapar V, Nieman LT, Solovyov A, Mehta A, Lieb DJ, Kulkarni AS, Jaicks C, Pinto CJ, Juric D, Chebib I, Colvin RB, Kim AY, Monroe R, Warren SE, Danaher P, Reeves JW, Gong J, Rueckert EH, Greenbaum BD, Hacohen N, Lagana SM, Rivera MN, Sholl LM, Stone JR, Ting DT, Deshpande V2020medRxivInfectious DiseaseMultiplex IHC
PD-L1 Expression and Clinical Outcomes to Cabozantinib, Everolimus, and Sunitinib in Patients with Metastatic Renal Cell Carcinoma: Analysis of the Randomized Clinical Trials METEOR and CABOSUN Flaifel A, Xie W, Braun DA, Ficial M, Bakouny Z, Nassar AH, Jennings RB, Escudier B, George DJ, Motzer RJ, Morris MJ, Powles, Wang E, Huang Y, Freeman GJ, Choueiri TK, Signoretti S2019Clinical Cancer ResearchImmuno-oncologyMultiplex IHC
The effect of cyfluthrin on testis inhibin B in rats and the intervention of Lycium barbarum polysaccharideGuo X, Xie Y-X, Guo C, Wei J-L, Yang H-F2021Molecular & Cellular ToxicologyToxicologyMultiplex IHC
Receptor tyrosine kinase expression and phosphorylation in canine nasal carcinoma Hocker SA, Higginbotham ML, Schermerhorn T, Henningson J2017Research in Veterinary ScienceOncologyMultiplex IHC
Multiplexed Immunohistochemistry for Molecular and Immune Profiling in Lung Cancer—Just About
Ready for Prime-Time?
Hofman P, Badoual C, Henderson F, Berland L, Hamila M, Long-Mira E, Lassalle S, Roussel H, Hofman V, Tartour E, Ilié M2019CancersOncology, Immuno-oncology, ReviewClassifier, Multiplex IHC
FOXM1 contributes to treatment failure in acute myeloid leukemia Khan I, Halasi M, Patel A, Schultz R, Kalakota N, Chen Y-H, Aardsma N, Liu L, Crispino JD, Mahmud N, Frankfurt O, Gartel AL2018JCI InsightOncologyMultiplex IHC
Digital image analysis improves precision of PD‐L1 scoring in cutaneous melanoma Koelzer VH, Gisler A, Hanhart JC, Griss J, Wagner SN, Willi N, Cathomas G, Sachs M, Kempf W, Thommen DS, Mertz KD2018HistopathologyOncology, Immuno-oncologyClassifier, Multiplex IHC
Precision immunoprofiling by image analysis and artificial intelligenceKoelzer VH, Sirinukunwattana K, Rittscher J, Mertz KD2019Vichows ArchivReviewClassifier, Multiplex IHC, Spatial Analysis
The role of WNT10B in normal prostate gland development and prostate cancerMadueke I, Hu W-Y, Hu D, Swanson SM, Griend DV, Abern M, Prins GS2019The ProstateOncologyMultiplex IHC
Immuno-Interface Score to Predict Outcome in
Colorectal Cancer Independent of Microsatellite
Instability Status
Nestarenkaite A, Fadhil W, Ramusson A, Susanti S, Hadjimichael E, Laurinaviciene A, Ilyas M, Laurinavicius A2020CancersImmuno-oncologyClassifier, Multiplex IHC
Two distinct immunopathological profiles in autopsy lungs of COVID-19Nienhold R, Ciani Y, Koelzer VH, Tzankov A, Haslbauer JD, Menter T, Schwab N, Henkel M, Frank A, Zsikla V, Willi N, Kempf W, Hoyler T, Barbareschi M, Moch H, Tolnay M, Cathomas G, Demichelis F, Junt T, Mertz KD2020medRxivImmunology, Infectious DiseaseMultiplex IHC, HALO AI
A single dose of peripherally infused EGFRvIII-directed CAR T cells mediates antigen loss and induces adaptive resistance in patients with recurrent glioblastoma O’Rourke DM, Nasrallah MP, Desai A, Melenhorst JJ, Mansfield K, MorrissetteJJD, Martinez-Lage M, Brem S, Maloney E, Shen A, Isaacs R, Mohan S, Plesa G, Lacey SF, Navenot J-M, Zheng Z, Levine BL, Okada H, June CH, Brogdon JL, Maus MV2017Science Translational MedicineOncology, Immuno-oncologyCytonuclear, Multiplex IHC
Assessment of associations between clinical and immune microenvironmental factors and tumor mutation burden in resected nonsmall cell lung cancer by applying machine learning to whole-slide imagesOno A, Terada Y, Kawata T, Serizawa M, Isaka M, Kawabata T, Imai T, Mori K, Muramatsu K, Hayashi I, Kenmotsu H, Oshima K, Urakami K, Nagashima T, Kusuhara M, Akiyama Y, Sugino T, Ohde Y, Yamaguchi K, Takahashi T T2020Cancer MedicineClassifier, Multiplex IHC, Serial Section
The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesisOser MG, Sabet AH, Gao W, Chakraborty AA, Schinzel AC, Jennings RB, Fonseca R, Bonal DM, Booker MA, Flaifel A, Novak JS, Christensen VL, Zhang H, Herbert ZT, Tolstorukov MY, Buss EJ, Wong K-K, Bronson RT, Nguyen Q-D, Signoretti S, Kaelin Jr WG2019Genes & DevelopmentOncologyMultiplex IHC
Immunogradient Indicators for Antitumor Response Assessment by Automated Tumor-Stroma Interface Zone DetectionRasmusson A, Zilenaite D, Nestarenkaite A, Augulis R, Laurinaviciene A, Ostapenko V, Poskus T, Laurinavicius A2020The American Journal of PathologyMultiplex IHC, HALO AI
Physiologic serum 1,25 dihydroxyvitamin D is inversely associated with prostatic Ki67 staining in a diverse sample of radical prostatectomy patients Rosenberg A, NetteY OS, Gogana P, Sheikh U, Virgilia M, Macias V, Kajdacsy-Balla A, R Sharifi R, Kittles RA, Murphy AB2019Cancer Causes & ControlOncologyMultiplex IHC
An Antibody Targeting ICOS Increases Intratumoral
Cytotoxic to Regulatory T-cell Ratio and Induces Tumor
Sainson RCA, Thotakura AK, Kosmac M, Borhis G, Parveen N, Kimber R, Carvalho J, Henderson SJ, Pryke KL, Okell T, O'Leary S, Ball S, Van Krinks C, Gamand L, Taggart E, Pring EJ, Ali H, Craig H, Wong VWY, Liang Q, Rowlands RJ, Lecointre M, Campbell J, Kirby I, Melvin D, Germaschewski V, Oelmann E, Quaratino S, McCourt M2020Cancer Immunology ResearchImmuno-oncologyMultiplex IHC
Nuclear Glycogenolysis Modulates Histone Acetylation in Human Non-Small Cell Lung CancersSun RC, Dukhande VV, Zhou Z, Young LEA, Emanuelle S, Brainson CF, Gentry MS2019Cell MetabolismOncologyMultiplex IHC
A transcriptionally and functionally distinct PD-1+ CD8+ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade Thommen DS, Koelzer VH, Herzig P, Roller A, Trefny M, Dimeloe S, Kiialainen A, Hanhart J, Schil C, Hess C, Prince SS, Wiese M, Lardinois D, Ho P-C, Klein C, Karanikas V, Mertz KD, Schumacher TN, Zippelius A2018Nature MedicineOncology, Immuno-oncologyMultiplex IHC
Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamstersTostanoski LH, Wegmann F, Martinot AJ, Loos C, McMahan K, Mercado NB, Yu J, Chan CN, Bondoc S, Starke CE, Nekorchuk M, Busman-Sahay K, Piedra-Mora C, Wrijil LM, Ducat S, Custers J, Atyeo C, Fischinger S, Burke JS, Feldman J, Hauser BM, Caradonna TM, Bondzie EA, Dagotto G, Gebre MS, Jacob-Dolan C, Lin Z, Mahrokhian SH, Nampanya F, Nityanandam R, Pessaint L, Porto M, ALi V, Benetiene D, Tevi K, Andersen H, Lewis MG, Schmidt AG, Lauffenburger DA, Alter G, Estes JD, Schuitemaker H, Zahn R, Barouch DH2020Nature MedicineInfectious DiseaseArea Quantification, Multiplex IHC
Inducing and exploiting vulnerabilities for the treatment of liver cancerWang C, Vegna S, Jin H, Benedict B, Lieftink C, Ramirez C, de Oliveira RL, Morris B, Gadiot J, Wang W, du Chatinier A, Wang L, Gao D, Evers B, Jin G, Xue Z, Schepers A, Jochems F, Sanchez AM, Mainardi S, te Riele H, Beijersbergen RL, Qin W, Akkari L, Bernards R2019NatureOncologyMultiplex IHC
Digital Immunophenotyping Predicts Disease Free and Overall Survival in Early Stage Melanoma PatientsDe Logu F, Galli F, Nassini R, Ugolini F, Simi S, Cossa M, Miracco C, Gianatti A, De Giorgi V, Rulli E, Cossu A, Massi D, Mandalà M2021CellsOncology, Immuno-oncologyMultiplex IHC
Activated Regulatory T-Cells, Dysfunctional and SenescentT-Cells Hinder the Immunity in Pancreatic CancerSivakumar S, Abu-Shah E, Ahern DJ, Arbe-Barnes EH, Jainarayanan AK, Mangal N, Reddy S, Rendek A, Easton A, Kurz E, Silva M, Soonawalla Z, Heij LR, Bashford-Rogers R, Middleton MR, DUstin ML2021cancersClassifier, Multiplex IHC, Spatial Analysis, Highplex FL
Age-associated expression of p21and p53 during human wound healingChia CW, Sherman-Baust CA, Larson SA, Pandey R, Withers R, Karikkineth AC, Zukley LM, Campisi J, Egan JM, Sen R, Ferrucci L2021Aging CellDermatologyArea Quantification, ISH/FISH, Multiplex IHC
Inhibition of STAT3 augments antitumor efficacy of anti-CTLA-4 treatment against prostate cancerWitt K, Evans-Axelsson S, Lundqvist A, Johansson M, Bjartell A, Hellsten R2021Cancer Immunology, ImmunotherapyImmuno-oncologyArea Quantification, Multiplex IHC
Tumor cell PD-L1 expression is a strong predictor of unfavorable prognosis in immune checkpoint therapy-naive clear cell renal cell cancerMöller K, Fraune C, Blessin NC, Lennartz M, Kluth M, Hube-Magg C, Lindhorst L, Dahlem R, Fisch M, Eichenauer T, Riechardt S, Simon R, Sauter G, Büscheck F, Höppner W, Matthies C, Doh O, Krech T, Marx AH, Zecha H, Rink M, Steurer S, Clauditz TS2021International Urology and NephrologyImmuno-oncologyMultiplex IHC, TMA
TNFα secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapyWei Z, Singh O, Ekinci C, Gill J, Li M, Mamatjan Y, Karimi S, Bunda S, Mansouri S, Aldape K, Zadeh G2021Acta Neuropathologica CommunicationsImmunology, NeuroscienceClassifier, Multiplex IHC
Mitigation of endemic GI-tract pathogen-mediated inflammation through development of multimodal treatment regimen and its impact on SIV acquisition in rhesus macaquesBochart RM, Busman-Sahay K, Bondoc S, Morrow DW, Ortiz AM, Fennessey CM, Fischer MB, Shiel O, Swanson T, Shriver-Munsch CM, Crang HB, Armantrout KM, Barber-Axthelm AM, Langner C, Moats CR, Labriola CS, MacAllister R, Axthelm MK, Brenchley JM, Keele BF, Estes JD, Hansen SG, Smedley JV2021PLOS PathogensInfectious DiseaseISH/FISH, Multiplex IHC
Analysis of genomic and non-genomic signaling of estrogen receptor in PDX models of breast cancer treated with a combination of the PI3K inhibitor alpelisib (BYL719) and fulvestrantJacquemetton J, Kassem L, Poulard C, Dahmani A, De Plater L, Montaudon E, Sourd L, Morisset L, El Botty R, Chateau-Joubert S, Vacher S, Bièche I, Treilleux I, Trédan O, Marangoni E, Le Romancer M2021Breast Cancer ResearchOncologyMultiplex IHC
Pathways of immune exclusion in metastatic osteosarcoma are associated with inferior patient outcomesLigon JA, Choi W, Cojocaru G, Fu W, Hsiue EH-C, Oke TF, Siegel N, Fong MH, Ladle B, Pratilas CA, Morris CD, Levin A, Rhee DS, Meyer CF, Tam AJ, Blosser R, Thompson ED, Suru A, McConkey D, Housseau F, Anders R, Pardoll DM, Llosa N2021Journal for ImmunoTherapy of CancerImmuno-oncologyMultiplex IHC
Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine releaseDang K, Castello G, Clarke S, et al.2021Journal for Immunotherapy of CancerImmuno-oncologyMultiplex IHC
Multiparametric Analyses of Hepatocellular Carcinoma Somatic Mouse Models and Their Associated Tumor MicroenvironmentTaranto D, Ramirez C, Vegna S, et al.2021Current ProtocolsOncologyMultiplex IHC
Rejection of benign melanocytic nevi by nevus-resident CD4+ T cellsSchiferle E, Cheon S, Ham S, Son H, Messerschmidt J, Lawrence D, Cohen J, Flaherty K, Moon J, Lian C, Sullivan R, Demehri S2021Science AdvancesOncologyMultiplex IHC
Extensive Three-Dimensional Intratumor Proteomic Heterogeneity Revealed by Multiregion Sampling in High-Grade Serous Ovarian Tumor SpecimensHunt A, Bateman N, Barakat W, Makohon-Moore S, Hood B, Conrads K, Zhou M, Calvert V, Pierobon M, Loffredo J, Litzi T, Oliver J, Mitchell D, Gist G, Rojas C, Blanton B, Robinson E, Odunsi K, Sood A, Casablanca Y, Darcy K, Schriver C, Petricoin E, Rao U, Maxwell G, Conrads T2021iScienceMultiplex IHC
TMEM229A suppresses non?small cell lung cancer progression via inactivating the ERK pathwayZhang X, He Y, Jiang Y, Bao Y, Chen Q, Xie D, Yu H, Wang X2021Oncology ReportsMultiplex IHC
Glucocorticoid receptor antagonism promotes apoptosis in solid tumor cellsGreenstein A, Hunt H2021OncotargetMultiplex IHC
A single-dose mRNA vaccine provides a long-term protection for hACE2 transgenic mice from SARS CoV-2Huang Q, Ji K, Tian S, Wang F, Huang B, Tong Z, Tan S, Hao J, Wang Q, Tan W, Gao G, Yan J2021Nature CommunicationsInfectious DiseaseMultiplex IHC
ACT1 is required for murine IL-23-induced psoriasiform inflammation potentially independent of E3 ligase activityLipovsky A, Slivka PF, Su Z, Wang Y, Paulsboe S, Wetter J, Namovic MT, Gauvin D, Perron D, Gauld S, McGaragughty S, Goedken ER2021Journal of Investigative DermatologyImmunologyMultiplex IHC
Pharmacologic modulation of RNA splicing enhances anti-tumor immunityLu S, De Neef E, Thomas J, Sabio E, Rousseau B, Gigoux M, Knorr D, Greenbaum B, Elhanti Y, Hogg S, Chow A, Ghosh A, Xie A, Zamarin D, Cui D, Erickson C, Singer M, Cho H, Wang E, Lu B, Durham B, Shah H, Chowell D, Gabel A, Shen Y, Liu J, Jin J, Rhodes M, Taylor R, Molina H, Wolchok J, Merghoub T, Diaz Jr. L, Abdel-Wahab O, Bradley B2021CellOncologyMultiplex IHC
Olaparib and durvalumab in patients with germline BRCA-mutated metastatic breast cancer (MEDIOLA): an open-label, multicentre, phase 1/2, basket studyDomchek SM, Postel-Vinay S, Im S-A, Park YH, Delord J-P, Italiano A, Alexandre J, You B, Bastian S, Krebs MG, Wang D, Waqar SN, Lanasa M, Rhee J, Gao H, Rocher-Ros V, Jones EV, Gulati S, Coenen-Stass A, Kozarewa I, Lai Z, Angell HK, Opincar L, Herbolsheimer P, Kaufman B2020The Lancet OncologyOncologyMultiplex IHC
ARID1A influences HDAC1/BRD4 activity, intrinsic proliferative capacity and breast cancer treatment responseNagarajan S, Rao SV, Sutton J, Cheeseman D, Dunn S, Papachristou EK, Prada J-EG, Couturier D-L, Kumar S, Kishore K, Chilamakuri CSR, Glont E, Goode EA, Brodie C, Guppy N, Natrajan R, Bruna A, Caldas C, Russel A, Siersbaek R, Yusa K, Chernukhin I, Carroll JS2020Nature GeneticsOncologyMultiplex IHC
Multimodal treatment combining cold atmospheric plasma and acidic fibroblast growth factor for multi?tissue regenerationTan F, Rui X, Xiang X, Yu Z, Al-Rubeai M2021The FASEB JournalOtherMultiplex IHC
ILC2-driven innate immune checkpoint mechanism antagonizes NK cell antimetastatic function in the lungSchuijs MJ, Png S, Richard AC, Tsyben A, Hamm G, Stockis J, Garcia C, Panaud S, Nicholls A, Ros XR, Su J, Eldridge MD, Riedel A, Serrao EM, Rodewald H-R, Mack M, Shields JD, Cohen ES, McKenzie ANJ, Goodwin RJA, Brindle KM, Marioni JC, Halim TYF2020Nature ImmunologyImmuno-oncologyMultiplex IHC
Imaging Tumor-Infiltrating Lymphocytes in Brain Tumors with [64Cu]Cu-NOTA-anti-CD8-Positron Emission TomographyNagle V, Henry K, Hertz C, et al.2021Clinical Cancer ResearchImmuno-oncologyMultiplex IHC
MAOA-mediated reprogramming of stromal fibroblasts promotes prostate tumorigenesis and cancer stemnessLi J, Pu T, Yin L, Li Q, Liao C-P, Wu BJ2020OncogeneOncologyMultiplex IHC
Intraductal pancreatic cancer is less responsive than cancer in the stroma to neoadjuvant chemotherapyFujikura K, Hutchings D, Braxton AM, Zhu Q, Laheru DA, Hruban RH, Thompson ED, Wood LD2020Modern PathologyOncologyMultiplex IHC
Development of a clinically relevant ovarian cancer model incorporating surgical cytoreduction to evaluate treatment of micro-metastatic diseaseMorse CB, Voillet V, Bates BM, Chiu EY, Garcia NM, Gottardo R, Greenberg PD, Anderson KG2020Gynecologic OncologyOncologyMultiplex IHC
Necroptosis in biliary atresia of the liverHashimoto M, Fujishima F, Lomphithak T, Jitkaew S, Nio M, Sasano H2021Medical Molecular MorphologyMetabolismMultiplex IHC
Baricitinib Treatment Resolves Lower-Airway Macrophage Inflammation and Neutrophil Recruitment in Sars-Cov-2-Infected Rhesus MacaquesHoang T, Pino M, Boddapati A, Viox E, Starke C, Upadhyay A, Gumber S, Nekorchuk M, Busman-Sahay K, Strongin Z, Harper J, Tharp G, Pellegrini K, Kirejczyk S, Zandi K, Tao S, Horton T, Beagle E, Mahar E, Lee M, Cohen J, Jean S, Wood J, Connor-Stroud F, Stammen R, Delmas O, Wang S, Cooney K, Sayegh M, Wang L, Filev P, Weiskopf D, Silvestri G, Waggoner J, Piantadosi A, Kasturi S, Al-Shakhshir H, Ribeiro S, Sekaly R, Levit R, Estes J, Vanderfold T, Schinazi R, Bosinger S, Paiardine M2021CellInfectious DiseaseMultiplex IHC
Large-Section Histopathology Can Better Indicate the Immune Microenvironment and Predict the Prognosis of Pancreatic Ductal Adenocarcinoma Than Small-Section HistopathologyDing G, Guo M, Yang Y, Sun C, Wu S, Liu X, Wang J, Jiang H, Liu Y, Zheng J2021Frontiers in OncologyOncologyMultiplex IHC
Suppression of insulin-induced gene 1 (INSIG1) function promotes hepatic lipid remodelling and restrains NASH progressionAzzu V, Vacca M, Kamzolas I, Hall Z, Leslie J, Carobbio S, Virtue S, Davies S, Lukasik A, Dale M, Bohlooly-Y M, Acharjee A, Linden D, Bidault G, Petsalaki E, Griffin J, Oakley F, Allison M, Vidal-Puig2021Molecular MetabolismMetabolismArea Quantification, Cytonuclear, Multiplex IHC
Inter-organ transmission of hepatocellular senescence induces multi-organ dysfunction through the TGF? signalling pathwayKiourtis C, Terradas-Terradas M, Gee L, Hsieh Y, Nixon C, Clark W, Shaw R, Hanson P, Sumpton D, Mackay G, May S, Muller M, Najumudeen A, Campbell A, Barry S, Morris C, LeBeau F, Sansom O, Kirschner K, Oakley F, Bird T2021Research SquareOtherArea Quantification, Cytonuclear, Multiplex IHC
IL6/STAT3 Signaling Hijacks Estrogen Receptor ? Enhancers to Drive Breast Cancer MetastasisSiersbæk R, Scabia V, Nagarajan S, Chernukhin I, Papachristou EK, Broome R, Johnston SJ, Joosten SEP, Green AR, Kumar S, Jones J, Omarjee S, Alvarez-Fernandez R, Glont S, Aitken SJ, Kishore K, Cheeseman D, Rakha EA, D'Santos C, Zwart W, Ruseel A, Brisken C, Carroll JS2020Cancer CellOncologyArea Quantification, Multiplex IHC
Over-expression of Th2-related molecules in the skin of patients with eosinophilic dermatosis of hematologic malignancyMaglie R, Ugolini F, De Logu F, Nassini R, Simi S, Nardiello P, Pasqualaini E, Baroini G, Del Bianco E, Massi D, Antiga E2021Journal of the American Academy of DermatologyOncology, Immuno-oncologyArea Quantification, Multiplex IHC
The RAC1 target NCKAP1 plays a crucial role in progression of BRAF/PTEN -driven melanoma in miceSwaminathan K, Campbell A, Papalazarou V, Jaber-Hijazi F, Nixon C, McGhee E, Strathdee D, Sansom OJ, Machesky LM2020Journal of Investigative DermatologyOncology, DermatologyArea Quantification, Multiplex IHC
The TRPA1 Channel Amplifies the Oxidative Stress Signal in MelanomaDe Logu F, Monteiro de Araujo D, Ugolini F, Iannone L, Vannucchi M, Portelli F, Landini L, Titiz M, De Giorgi V, Geppetti P, Massi D, Nassini R
2021CellsArea Quantification, Multiplex IHC, HALO AI
p53-mediated redox control promotes liver regeneration and maintains liver function in response to CCl4Humpton T, Hall H, Kiourtis C, Nixon C, Clark W, Hedley A, Shaw R, Bird T, Blyth K, Vousden K2021Cell Death & DifferentiationMetabolismCytonuclear, Multiplex IHC
Combined CTLA-4 and PD-L1 blockade in patients with chemotherapy-naïve metastatic castration-resistant prostate cancer is associated with increased myeloid and neutrophil immune subsets in the bone microenvironmentSubudhi S, Siddiqui B, Aparicio A, Yadav S, Basu S, Chen H, Jindal S, Tidwell R, Varma A, Logothetis C, Allison J, Corn P, Sharma P2021Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Multiplex IHC
Development of a novel humanized mouse model for improved evaluation of in vivo anti-cancer effects of anti-PD-1 antibodyKatano I, Hanazawa A, Otsuka I, Yamaguchi T, Mochizuki M, Kawai K, Ito R, Goto M, Kagawa T, Takahashi T2021Scientific ReportsOncologyCytonuclear, Multiplex IHC
A comparability study of natural and deglycosylated PD-L1 levels in lung cancer: evidence from immunohistochemical analysisMei J, Xu J, Yang X, Gu D, Zhou W, Wang H, Liu C2021Molecular CancerOncologyCytonuclear, Multiplex IHC
A faithful in vivo model of human macrophages in metastatic melanomaVoillet V, Berger T, McKenna K, Paulson K, Smythe K, Hunter D, Valente W, Weaver S, Campbell J, Kim T, Byrd D, Bielas J, Pierce R, Chapuis A, Gottardo R, Rongvaux2021bioRxivCytonuclear, Multiplex IHC
E3 ligase-inactivation rewires CBL interactome to elicit oncogenesis by hijacking RTK–CBL–CIN85 axisAhmed S, Buetow L, Gabrielsen M, Lilla S, Sibbet G, Sumpton D, Zanivan S, Hedley A, Clark W, Huang D2021OncogeneOncologyCytonuclear, Multiplex IHC
CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulationLe A, Yelland T, Paul N, Fort L, Nikolaou S, Ismail S, Machesky L2021Journal of Cell BiologyOtherCytonuclear, Multiplex IHC
In vitro amplification of pathogenic tau conserves disease-specific bioactive characteristicsXu H, O'Reilly M, Gibbons G, Changolkar L, McBride J, Riddle D, Zhang B, Stieber A, Nirschl J, Kim S, Hoxha K, Brunden K, Schellenberg G, Trojanowski J, Lee V2021Acta NeuropathologicaNeruoscienceCytonuclear, Multiplex IHC
CCNE1 amplification is synthetic-lethal with PKMYT1 kinase inhibitionGallo D, Young J, Fourtounis J, Martino G, Alvarez-Quilon A, Bernier C, Duffy N, Papp R, Roulston A, Stocco R, Szychowski J, Veloso A, Alam H, Baruah P, Fortin A, Bowlan J, Chaudhary N, Desjardins J, Dietrich E, Fournier S, Fugere-Desjardins C, de Rugy T, Leclaire M, Liu B, Melo H, Nicolas O, Singhania A, Szilard R, Tkac J, Yin S, Morris S, Zinda M, Marshall C, Duocher D2021bioRxivOtherCytonuclear, Multiplex IHC
Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicityQiao Y, Qiu Y, Ding J, Luo N, Wang H, Ling X, Sun J, Wu Z, Wang Y, Liu Y, Guo F, Sun T, Shen W, Zhang M, Wu D, Chen B, Xu W, Wang X2021Nature CommunicationsImmuno-oncologyCytonuclear, Multiplex IHC
Pixelwise H-score: a novel digital image analysis-based metric to quantify membrane biomarker expression from immunohistochemistry imagesRam S, Vizcarra P, Whalen P, Deng S, Painter C, Jackson-Fisher A, Pirie-Shepherd S, Xia X, Powell E2021PLOS OneOtherCytonuclear, Multiplex IHC
LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesisWei W, Wang N, Deng M, Dong P, Liu J, Xiang Z, Li X, Li Z, Liu Z, Peng Y, Li Z, Jiang L, Yao K, Ye Y, Lu W, Zhang Z, Zhou F, Liu Z, Xie D, Yu C2021Redox BiologyMetabolismCytonuclear, Multiplex IHC
Hedgehog-signalling in papillary fibroblasts is essential for hair follicle regeneration during wound healingFrech S, Forsthuber A, Korosec A, Lipp K, Kozumov V, Lichtenberger B2021Journal of Investigative DermatologyDermatologyCytonuclear, Multiplex IHC
Evaluation of Early Markers of Ischemia-reperfusion Injury and Preservation Solutions in a Modified Hindlimb Model of Vascularized Composite AllotransplantationRostami S, Xu M, Datta S, Haykal S2022Transplantation DirectCytonuclear, Multiplex IHC
B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinomaYamada T, Miki Y, Suzuki M, Kondoh O, Saito-Koyama R, Ono K, Okada Y, Sasano H2021Cancer MedicineOncologyCytonuclear, Multiplex IHC
Discoidin Domain Receptor 1 (DDR1) is Necessary for Tissue Homeostasis in Pancreatic Injury and Pathogenesis of Pancreatic Ductal AdenocarcinomaRuggeri JM, Franco-Barraza J, Sohail A, Zhang Y, Long D, di Magliano MP, Cukierman E, Fridman R, Crawford HC2020The American Journal of PathologyOncology, ImmunologyCytonuclear, Multiplex IHC, Highplex FL
CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta, alpha, beta, and gammaGeng J, Chen L, Yuan Y, Wang K, Wang Y, Qin C, Wu G, Chen R, Zhang Z, Wei D, Du P, Zhang J, Lin P, Zhang K, Deng Y, Xu K, Liu J, Sun X, Guo T, Yang X, Wu J, Jiang J, Li L, Zhang K, Wang Z, Zhang J, Yan Q, Zhu H, Zheng Z, Miao J, Fu X, Yang F, Chen X, Tang H, Zhang Y, Shi Y, Zhu Y, Pei Z, Huo F, Liang X, Wang Y, Wang Q, Xie W, Li Y, Shi M, Bian H, Zhu P, Chen Z2021Signal Transduction and Targeted TherapyInfectious DiseaseCytonuclear, Multiplex IHC, Highplex FL
Cross-linking of T cell to B cell lymphoma by the T cell bispecific antibody CD20-TCB induces IFN?/CXCL10-dependent peripheral T cell recruitment in humanized murine modelCremasco F, Menietti E, Speziale D, Sam J, Sammicheli S, Richard M, Varol A, Klein C, Umana P, Bacac M, Colombetti S, Perro M2021PLOS ONEOncologyCytonuclear, Multiplex IHC, Highplex FL
mRNA translation is a therapeutic vulnerability necessary for bladder epithelial transformationJana S, Deo R, Hough R, et al.2021JCI InsightOncologyMultiplex IHC, Highplex FL
Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint BlockadeSrivastava S, Furlan SN, Jaeger-Ruckstuhl CA, Sarvothama M, Berger C, Smythe KS, Garrison SM, Specht JM, Lee SM, Amezquita RA, Voillet V, Muhunthan V, Yechan-Gunja S, Pillai SPS, Rader C, Houghton AM, Pierce RH, Gottardo R, Maloney DG, Riddell SR2020Cancer CellImmuno-oncologyMultiplex IHC, Highplex FL
STAT3 antisense oligonucleotide remodels the suppressive tumor microenvironment to enhance immune activation in combination with anti-PD-L1Proia T, Singh M, Woessner R, Carnevalli LS, Bommakanti G, Magiera L, Srinivasan SS, Grosskurth SE, Collins M, Womack C, Griffin M, Ye M, Cantin S, Russell DL, Xie M, Hughes AM, Deng N, Mele DA, Fawell SE, Barry ST, Reimer C, Barrett JC, McCoon P2020Clinical Cancer ResearchImmuno-oncologyMultiplex IHC, Highplex FL
Neonatal hydrocephalus leads to white matter neuroinflammation and injury in the corpus callosum of Ccdc39 hydrocephalic miceGoulding DS, Vogel RC, Pandya CD, Shula C, Gensel JC, Mangano FT, Goto J, Miller BA2020Journal of NeurosurgeryImmunology, NeuroscienceMultiplex IHC, Highplex FL
Immune evasion before tumour invasion in early lung squamous carcinogenesis Mascaux C, Angelova M, Vasaturo A, Beane J, Hijazi K, Anthoine G, Rothe F, Willard-Gallo K, Haller A, Ninane V, Burny A, Sculier J-P, Spira A, Galon J2019NatureOncology, Immuno-oncologyMultiplex IHC
Cooperative targeting of immunotherapy-resistant melanoma and lung cancer by an AXL-targeting antibody-drug conjugate and immune checkpoint blockadeBoshuizen J, Pencheva N, Krijgsman O, Altimari DD, Castro PG, de Bruijn B, Ligtenberg MA, den Heuvel EG, Vredevoogd DW, Song J-Y, Visser N, Apriamashvili G, Janmaat ML, Plantinga TS, Franken P, Houtkamp M, Lingnau A, Jure-Kunkel M, Peeper DS2021Cancer ResearchImmuno-oncologyMultiplex IHC
Intratumoral Heterogeneity and Immune Response Indicators to Predict Overall Survival in a Retrospective Study of HER2-Borderline (IHC 2+) Breast Cancer PatientsRadziuviene G, Rasmusson A, Augulis R, Grineviciute R, Zilenaite D, Laurinaviciene A, Ostapenko V, Laurinavicius A2021Frontiers in OncologyImmuno-oncologyMembrane, Multiplex IHC, HALO AI
Two distinct immunopathological profiles in autopsy lungs of COVID-19Nienhold R, Ciani Y, Koelzer V, Tzankov A, Haslbauer J, Menter T, Schwab N, Henkel M, Frank A, Zsikla V, Willi N, Kempf W, Hoyler T, Barbareschi M, Moch H, Tolnay M, Cathomas G, Dernichelis F, Junt T, Mertz K2021Nature CommunicationsInfectious DiseaseMultiplex IHC, HALO AI
Tertiary lymphoid structures critical for prognosis in endometrial cancer patients - a TransPORTEC studyHoreweg N, Workel, H, Loiero D, Church D, Vermij L, Léon-Castillo A, Krog R, de Boer S, Nout R, Powel M, Mileshkin L, MacKay H, Leary A, Singh N, Jürgenliemk-Schulz I, Creutzberg C, Koelzer V, Nijman H, Bosse T, Bruyn M2021Research SquareMultiplex IHC, HALO AI, TMA
Endothelial cell infection and dysfunction, immune activation in severe COVID-19Qin Z, Liu F, Blair R, Wang C, Yang H, Mudd J, Currey J, Iwanaga N, He J, Mi R, Han K, Midkiff C, Alam M, Aktas B, Heide R, Veazey R, Piedimonte G, Maness N, Ergün S, Mauvais-Jarvis F, Rappaport J, Kolls J, Qin X2021TheranosticsImmunology, Infectious DiseaseMultiplex IHC, Spatial Analysis
Establishing standardized immune phenotyping of metastatic melanoma by digital pathologySobottka B, Nowak M, Frei A, Haberecker M, Merki S, Tumor Profiler consortium, Levesque M, Dummer R, Moch H, Koelzer V2021Laboratory InvestigationImmuno-oncologyMultiplex IHC, Spatial Analysis, HALO AI
Genomic analysis of Vascular Invasion in Hepatocellular Carcinoma (HCC) Reveals Molecular Drivers and Predictive BiomarkersKrishnan MS, Rajan KD A, Park J, Arjunan V, Marques FJG, Bermudez A, Girvan OA, Hoang NS, Yin J, Nguyen MH, Kothary N, Pitteri S, Felsher DW, Dhanasekaran R2020HepatologyOncologyMultiplex IHC, TMA
B7-H3 immune checkpoint expression is a poor prognostic factor in colorectal carcinomaLu Z, Zhao Z-X, Cheng P, Huang F, Guan X, Zhang M-G, Chen H-P, Liu Z, Jiang Z, Zheng Z-X, Zou S-M, Wang X-S2020Modern PathologyImmuno-oncologyMultiplex IHC, TMA
A preclinical trial and molecularly-annotated patient cohort identify predictive biomarkers in homologous recombination deficient pancreatic cancerWang Y, Park J, Pacis A, Denroche R, Jang G, Zhang A, Cuggia A, Domecq C, Monlong J, Raitses-Gurevich M, Grant R, Borgida A, Holter S, Stossel C, Bu S, Masoomian M, Lungu I, Bartlett J, Wilson J, Gao Z, Riazalhosseini Y, Asselah J, Bouganim N, Cabrera T, Boucher L, Valenti D, Biagi J, Greenwood C, Polak P, Foulkes W, Golan T, O'Kane G, Fischer S, Knox J, Gallinger S, Zogopoulos G2020Clinical Cancer ResearchOncologyClassifier, Cytonuclear, Multiplex IHC
Glucagon Receptor Inhibition Reduces Hyperammonemia and Lethality in Male Mice with Urea Cycle DisorderCavino K, Sung B, Su Q, Na E, Kim J, Cheng X, Gromada J, Okamoto H2020EndocrinologyMetabolismISH/FISH, Multiplex IHC
Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effectsEscuin-Ordinas H, Liu Y, Hufo W, Dimatteo R, Huang R, Krystofinski P, Azhdam A, Lee J, Comin-Anduix B, Cochran A, Lo R, Segura T, Scumpia P, Ribas A2021PLOS ONEDermatologyObject Colocalization, Multiplex IHC
Specificity and off-target effects of AAV8-TBG viral vectors for the manipulation of hepatocellular gene expression in miceKiourtis C, Wilczynska A, Nixon C, Clark W, May S, Bird T2021Biology OpenOtherMultiplex IHC
Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivationWu H, He P, Ren Y, Xiao S, Wang W, Liu Z, Li H, Wang Z, Zhang D, Cai J, Zhou X, Jiang D, Fei X, Zhao L, Zhang H, Liu Z, Chen R, Li W, Wang C, Zhang S, Qin J, Nasan B, Sun C2022Nature CommunicationsInfectious DiseaseMultiplex IHC
Transcription factor Mesenchyme Homeobox Protein 2 (MEOX2) modulates nociceptor functionKokotovic T, Lenartowicz E, Langeslag M, Ciotu C, Fell C, Scaramuzza A, Fischer M, Kress M, Penninger J, Nagy V2022The FEBS JournalNeruoscienceMultiplex IHC
The microglial P2Y6 receptor mediates neuronal loss and memory deficits in neurodegenerationPuigdellivol M, Milde S, Vilalta A, Cockram T, Allendorf D, Lee J, Dundee J, Pampuscenko K, Borutaite V, Nuthall H, Brelstaff J, Spillantini G, Brown G2021Cell ReportsNeuroscienceArea Quantification, Cytonuclear, Multiplex IHC
An Fc-inert PD-L1×4-1BB bispecific antibody mediates potent anti-tumor immunity in mice by combining checkpoint inhibition and conditional 4-1BB co-stimulationMuik A, Altintas I, Gieseke F, Schoedel K, Burm S, Toker A, Salcedo T, Verzijl, Eisel D, Grunwitz C, Kranz L, Vormehr M, Satijn D, Diken M, Kreiter S, Sasser K, Ahmadi T, Tureci O, Breij E, Jure-Kunkel M, Sahin U2022OncoImmunologyImmuno-oncologyArea Quantification, Immune, Multiplex IHC
An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancerVoabil P, de Bruijn M, Roelofsen L, Hendriks S, Brokamp S, van den Braber M, Broeks A, Sanders J, Herzig P, Zippelius A, Blank C, Hartemink K, Monkhorst K, Haanen J, Schumacher T, Thommen D2021Nature MedicineImmuno-oncologyClassifier, Multiplex IHC
Investigation of Breast Cancer Microstructure and Microvasculature from Time-Dependent DWI and CEST in Correlation with Histological BiomarkersSomeya Y, Iima M, Imai H, Yoshizawa A, Kataoka M, Isoda H, Le Bihan D, Nakamoto Y2021Research SquareClassifier, Multiplex IHC
A selective and orally bioavailable VHL-recruiting PROTAC achieves SMARCA2 degradation in vivoKofink C, Trainor N, Mair B, Wrhrle S, Wurm M, Mischerikow N, Bader G, Rumpel K, Gerstberger T, Cui Y, Greb P, Garavel G, Scharnweber M, Fuchs J, Gremel G, Chette P, Hopf S, Budano N, Rinnenthal J, Gmaschitz G, Diers E, McLennan R, Roy M, Whitworth C, Vetma V, Mayer M, Koegl M, Ciulli A, Weinstabl H, Farnaby W2022Biological and Medicinal ChemistryClassifier, Cytonuclear, Multiplex IHC
Pancreas resident macrophage-induced fibrosis has divergent roles in pancreas inflammatory injury and PDACBaer J, Zuo C, Kang L, Alarcon de la Lastra A, Bocherding N, Knolhoff B, Bogner S, Zhu Y, Lewis M, Zhang N, Kim K, Fields R, Mills J, Ding L, Randolph G, DeNardo D2022bioRxivFibrosisClassifier, Area Quantification, Cytonuclear, Multiplex IHC, Highplex FL, Figure Maker
Anti-CAIX BB? CAR4/8 T Cells Exhibit Superior Efficacy in a Clear Cell Renal Cell Carcinoma (ccRCC) Mouse ModelWang Y, Buck A, Grimaud M, Culhane A, Kodangattil S, Razimbaud C, Bonal D, Nguyen Q, Zhu Z, Wei K, O'Donnell M, Huang Y, Signoretti S, Choueiri T, Freeman G, Zhu Q, Marasco2021Molecular Therapy: OncolyticsOncologyCytonuclear, Multiplex IHC
Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancerZheng S, Zou Y, Tang Y, Yang A, Liang J, Wu L, Tian W, Xiao W, Xie X, Yang L, Xie J, Wei W, Xie X2022OncoImmunologyImmuno-oncologyCytonuclear, Multiplex IHC
Collaborative workflow between pathologists and deep learning for evaluation of tumor cellularity in lung adenocarcinomaSakamoto T, Furukawa T, Pham H, Kuroda K, Tabata K, Kshima Y, Okoshi E, Morimoti S, Bychkov A, Fukuoka J2022bioRxivOncologyCytonuclear, Multiplex IHC
Pathogenesis of follicular thymic hyperplasia associated with rheumatoid arthritisOhe R, Yang S, Yamashita D, Ichikawa C, Saito A, Kabasawa T, Utsunomiya A, Aung N, Urano Y, Kitaoka T, Suzuki K, Takahara D, Sasaki A, Takakubo Y, Takagi M, Yamakawa M, Futakuchi M2022Pathology InternationalOtherCytonuclear, Multiplex IHC
Nuclear expression of pSTAT3Tyr705 and pSTAT3Ser727 in the stromal compartment of localized hormone-naïve prostate cancerMarginean F, Hellsten R, Krzyanowska A, Bjartell A2022Pathology - Research and PracticeOncologyCytonuclear, Multiplex IHC, TMA
Tertiary lymphoid structures critical for prognosis in endometrial cancer patients - a TransPORTEC studyHoreweg N, Workel, H, Loiero D, Church D, Vermij L, Léon-Castillo A, Krog R, de Boer S, Nout R, Powel M, Mileshkin L, MacKay H, Leary A, Singh N, Jürgenliemk-Schulz I, Creutzberg C, Koelzer V, Nijman H, Bosse T, Bruyn M2022Nature CommunicationsOncologyCytonuclear, Multiplex IHC, TMA
ZEB1 transcription factor promotes immune escape in melanomaPlaschka M, Benboubker V, Grimont M, Berthet J, Tonon L, Lopez J, Le-Bouar M, Balme B, Tondeau G, de la Fouchardiere A, Larue L, Puisieux A, Grinberg-Bleyer Y, Bendriss-Vermare N, Dubois B, Caux C, Dalle S, Caramel J2022Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Multiplex IHC, Highplex FL
Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplasticaHong Y, Shan S, Gu Y, Huang H, Zhang Q, Han Y, Dong Y, Liu Z, Huang M, Ren T2022Nature CommunicationsOtherCytonuclear, Multiplex IHC
Sulforaphane exhibits antiviral activity against pandemic SARS-CoV-2 and seasonal HCoV-OC43 coronaviruses in vitro and in miceOrdonez A, Bullen C, Villabona-Rueda A, Thompson E, Turner M, Merino V, Yan Y, Kim J, Davis S, Komm O, Powell J, D'Alessio F, Yolken R, Jain S, Jones-Brando L2022Communications BiologyInfectious DiseaseArea Quantification, Multiplex IHC
Interspatial Distribution of Tumor and Immune Cells in Correlation with PD-L1 in Molecular Subtypes of Gastric CancersDislich B, Mertz K, Gloor B, Langer R2022CancersImmuno-oncologyMultiplex IHC, Spatial Analysis, Figure Maker
Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaquesAid M, Vidal S, Piedra-Mora C, Ducat S, Chan C, Bondoc S, Colarusso A, Starke C, Nekorchuk M, Busman-Sahay K, Estes J, Martinot A, Barouch D2022PLOS PathogensInfectious DiseaseArea Quantification, Multiplex IHC
Hyperpolarized 13C-Pyruvate Metabolism as a Surrogate for Tumor Grade and Poor Outcome in Renal Cell Carcinoma—A Proof of Principle StudyUrsprung S, Woitek R, McLean M, Priest A, Crispin-Ortuzar M, Brodie C, Gill A, Gehrung M, Beer L, Riddick A, Field-Rayner J, Grist J, Deen S, Riemer F, Kaggie J, Zaccagna F, Duarte J, Locke M, Frary A, Aho T, Armitage J, Casey R, Mendichovszky I, Welsh S, Barrett T, Graves M, Eisen T, Mitchell T, Warren A, Brindle K, Sala E, Stewart G, Gallagher F2022CancersOncologyArea Quantification, Cytonuclear, Multiplex IHC
Therapeutic impact of BET inhibitor BI 894999 treatment: backtranslation from the clinicTontsch-Grunt U, Traexler P, Baum A, Musa H, Marzin K, Wang S, Trapani F, Engelhardt H, Solca F2022British Journal of CancerOncologyClassifier, Cytonuclear, Multiplex IHC
Organelle resolved proteomics reveals new chordoma cell surface markers required for proliferation and association with outcomeKhan S, Zuccato J, Ignatchenko V, Singh O, Govindarajan M, Waas M, Gao A, Zadeh G, Kislinger T2022Research SquareOncologyCytonuclear, Multiplex IHC
Immune cell subsets in interface cutaneous immune-related adverse events (cirAEs) associated with anti-PD-1 therapy resemble accute graft vs host disease more than licen planusCruz G, Kaunitz G, Stein J, Sander I, Hollmann T, Cottrell T, Taube J, Sunshine J2022Journal of Cutaneous PathologyImmunologyCytonuclear, Multiplex IHC
Immune and epithelial determinants of age-related risk and alveolar injury in fatal COVID-19Chait M, Yilmaz M, Shakil S, Ku A, Dogra P, Connors T, Szabo P, Gray J, Wells S, Kubota M, Matsumoto R, Poon M, Snyder M, Baldwin M, Sims P, Saqi A, Farber D, Weisberg S2022JCI InsightInfectious DiseaseMultiplex IHC
C57BL/6J Mice Are Not Suitable for Modeling Severe SARS-CoV-2 Beta and Gamma Variant InfectionCurrey J, Rabito F, Maness N, Blair R, Rappaport J, Qin X, Kolls J, Srivastava A2022VirusesInfectious DiseaseCytonuclear, Multiplex IHC
Type I interferon regulates proteolysis by macrophages to prevent immunopathology following viral infectionLee A, Feng E, Chew M, Balint E, Poznanski S, Giles E, Zhang A, Marzok A, Revill S, Vahedi F, Dubey A, Ayaub E, Jimenez-Saiz R, McGrath J, Ritchie T, Jordana M, Jonigk D, Ackermann M, Ask K, Miller M, Richards C, Ashkar A2022PLOS PathogensInfectious DiseaseArea Quantification, Multiplex IHC, TMA
Dietary Pharmacological Zinc and Copper Enhances Voluntary Feed Intake of Nursery PigsDeMille C, Burrough E, Kerr B, Schweer W, Gabler N2022Frontiers in Animal ScienceOtherCytonuclear, Multiplex IHC
Spatial transcriptome of a germinal center plasmablastic burst hints at MYD88/CD79B mutants-enriched diffuse large B-cell lymphomasL'Imperio V, Morello G, Vegliante M, Cancila V, Bertolazzi G, Mazzara S, Belmonte B, Mangogna A, Balzarini P, Corral L, Lopez G, Di Napoli A, Facchetti F, Pagni F, Tripodo C2022European Journal of ImmunologyOncologyCytonuclear, Multiplex IHC
Multi-scale integrative analyses identify THBS2+ cancer-associated fibroblasts as a key orchestrator promoting aggressiveness in early-stage lung adenocarcinomaYang H, Sun B, Fan L, Ma W, Xu K, Hall S, Wang Z, Schmid R, Peng R, Marti T, Gao W, Xu J, Yang W, Yao F2022TheranosticsOncologyCytonuclear, Multiplex IHC
Maternal Western-Style Diet Impairs Bone Marrow Development and Drives a Hyperinflammatory Phenotype in Hematopoietic Stem and Progenitor Cells in Fetal Rhesus MacaquesSureshchandra S, Chan C, Robino J, Parmelee L, Nash M, Wesolowski S, Pietras E, Friedman J, Takahashi D, Shen W, Hennebold J, Goldman D, Packwood W, Lindner J,Roberts C, Burwitz B, Messaoudi I, Varlamov O2022bioRxivMetabolismMultiplex IHC
Preclinical evaluation of FAP?2286 for fbroblast activation protein targeted radionuclide imaging and therapyZboralski D, Hoehne A, Bredenbeck A, Schumann A, Nguyen M, Schneider E, Ungewiss J, Paschke M, Haase C, von Hacht J, Kwan T, Lin K, Lenore J, Harding T, Xiao J, Simmons A, Mohan A, Beindorff N, Reineke U, Smerling C, Osterkamp F2022European Journal of Nuclear Medicine and Molecular ImagingOncologyCytonuclear, Multiplex IHC
Prognostic Integrated Image-Based Immune and Molecular Profiling in Early-Stage Endometrial CancerHoreweg N, de Bruyn M, Nout R, Stelloo E, Kedzierska K, Leon-Castillo A, Plat A, Mertz K, Osse M, Jurgenliemk-Schulz I, Lutgens L, Jobsen J, van der Steen-Banasik E, Smit V, Creutzberg C, Bosse T, Nijman H, Koelzer V, Church D2020Cancer Immunology ResearchOncologyCytonuclear, Multiplex IHC, TMA
Randomized Controlled Trial of the Gastrin/CCK2 Receptor Antagonist Netazepide in Patients with Barrett's EsophagusAbrams J, Del Portillo A, Hills C, Compres G, Friedman R, Cheng B, Poneros J, Lightdale C, Del La Rue R, di Pietro M, Fitzgerald R, Sepulveda A, Wang T2021Cancer Prevention ResearchCytonuclear, Multiplex IHC
Independent Prognostic Value of Intratumoral Heterogeneity and Immune Response Features by Automated Digital Immunohistochemistry Analysis in Early Hormone Receptor-Positive Breast CarcinomaZilenaite D, Rasmusson A, Augulis R, Besuparis J, Laurinaviciene A, Plancoulaine B, Ostpenko V, Laurinavicius2020Frontiers in OncologyOncologyMultiplex IHC, Figure Maker
Integrated Analysis Of Immunotherapy Treated Clear Cell Renal Cell Carcinomas: An Exploratory StudySobottka B, Nienhold R, Nowak M, Hench J, Haeuptle P, Frank A, Sachs M, Kahraman A, Moch H, Koelzer V, Mertz K2022Journal of ImmunotherapyImmuno-oncologyCytonuclear, Multiplex IHC, Figure Maker
A lncRNA signature associated with tumor immune heterogeneity predicts distant metastasis in locoregionally advanced nasopharyngeal carcinomaLiang Y, Zhang Y, Tan X, Qiao H, Liu S, Tang L, Mao Y, Chen L, Li W, Zhou G, Zhao Y, Li J, Li Q, Huang S, Gong S, Zheng Z, Li Z, Sun Y, Jiang W, Ma J, Li Y, Liu N2022Nature CommunicationsImmuno-oncologyClassifier, Multiplex IHC, Registration
Prognostic image-based quantification of CD8CD103 T cell subsets in high-grade serous ovarian cancer patientsPaijens S, Vledder A, Loiero D, Duiker E, Bart J, Hendriks A, Jalving M, Workel H, Hollema H, Werner N, Plat A, Wisman G, Yigit R, Arts H, Kruse A, de Lange N, Koelzer V, Bruyn M, Nijman H2021OncoImmunologyImmuno-oncologyCytonuclear, Multiplex IHC, TMA
Prenatal di-(2-ethylhexyl_ phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal miceYu D, Zhu D, Wang X, Li B, Li J, Lu P, Ji Y, Wang X2022Ecotoxicology and Environmental SafetyOtherMultiplex IHC
Effects of Extracorporeal Shockwave Therapy on Functional Recovery and Circulating miR-375 and miR-382-5p after Subacute and Chronic Spinal Cord Contusion Injury in RatsAshmwe M, Posa K, Ruhrnossl A, Heinzel J, Heimel P, Mock M, Schadl B, Keibl C, Couillard-Despres S, Redl H, Mittermayr R, Hercher D2022BiomedicinesNeuroscience, OtherClassifier, Multiplex IHC
Spatial meta-transcriptomics reveal associations of intratumor bacteria burden with lung cancer cells showing a distinct oncogenic signatureWong-Rolle A, Dong Q, Zhu Y, Divakar P, Hor J, Kedei N, Wong M, Tillo D, Conner E, Rajan A, Schrump D, Jim C, Germain R, Zhao C2022Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Multiplex IHC
SOX9+ malignant cells define a poor prognosis subtype of Glioma with tumor-associated macrophage recruitmentYu S, Ma C, Wang H, Yu B, Luan Y, Xia Z2022Research SquareImmuno-oncologyCytonuclear, Multiplex IHC
The combination of gene hyperamplification and PD-L1 expression as a biomarker for the clinical benefit of tislelizumab in gastric/gastroesophageal junction adenocarcinomaLu Z, Yang S, Luo X, Shi Y, Lee J, Deva S, Liu T, Chao Y, Zhang Y, Huang R, Xu Y, Shen Z, Shen L2022Gastric CancerImmuno-oncologyClassifier, Multiplex IHC
Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrixKay E, Paterson K, Riero-Domingo C, Sumpton D, Dabritz J, Tardito S, Boldrini C, Hernandez-Fernaud J, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson L, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson R, Park M, Kirschner K, Miller C, Kamphorst J, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S2022Nature MetabolismOncology, MetabolismCytonuclear, Multiplex IHC, Highplex FL
Discovery of a novel ALK/ROS1/FAK inhibitor, APG-2449, in preclinical non-small cell lung cancer and ovarian cancer modelsFang D, Tao R, Wang G, Li Y, Zhang K, Xu C, Zhai G, Wang Q, Wang J, Tang C, Min P, Xiong D, Chen J, Wang S, Yang D, Zhai Y2022BMC CancerOncologyMultiplex IHC
Cell graph neural networks enable the precise prediction of patient survival in gastric cancerWang Y, Wang Y, Hu C, Li M, Fan Y, Otter N, Sam I, Gou H, Hu Y, Kwok T, Zalcberg J, Boussioutas A, Daly R, Montufar G, Lio P, Xu D, Webb G, Song J2022Precision OncologyOncologyMultiplex IHC
Spartalizumab or placebo in combination with dabrafenib and trametinib in patients with BRAF V600-mutant melanoma: exploratory biomarker analyses from a randomized phase 3 trial (COMBI-i)Tawbi H, Robert C, Brase J, Gusenleitner D, Gasal E, Garrett J, Savchenko A, Gorgun G, Flaherty K, Ribas A, Dummer R, Schadendorf D, Long G, Nathan P, Ascierto P2022Journal for ImmunoTherapy of CancerOncologyMultiplex IHC, Spatial Analysis
Approaches to spatially resolving the tumour immune microenvironment of hepatocellular carcinomaMarsh-Wakefield F, Ferguson A, Liu K, Santhakumar C, McCaughan G, Palendira U2022Therapeutic Advances in Medical OncologyReviewMultiplex IHC, Spatial Analysis, Highplex FL
Intact HIV proviruses persist in the brain despite viral suppression with ARTCochrane C, Angelovich T, Byrnes S, Waring E, Guanizo A, Trollope G, Zhou J, Vue J, Senior L, Wanicek E, Eddine J, Gartner M, Jenkins T, Gorry P, Brew B, Lewin S, Estes J, Roche M, Churchill M2022Annals of NeurologyInfectious DiseaseMultiplex IHC
Age-associated expression of p21 and p53 during human wound healingChia CW, Sherman-Baust CA, Larson SA, Pandey R, Withers R, Karikkineth AC, Zukley LM, Campisi J, Egan JM, Sen R, Ferrucci L2021Aging CellDermatologyArea Quantification, ISH/FISH, Multiplex IHC
Radioprotective effect of X-ray abdominal FLASH irradiation: Adaptation to oxidative damage and inflammatory response may be benefiting factorsZhu H, Xie D, Yang Y, Huang S, Gao X, Peng Y, Wang B, Wang J, Xiao D, Wu D, Li C, Li C, Qian C, Deng X2022Medical PhysicsOncology, OtherMultiplex IHC
Upregulation of C/EBPa Inhibits Suppressive Activity of Myeloid Cells and Potentiates Antitumor Response in Mice and Patients with CancerHashimoto A, Sarker D, Reebye V, Jarvis S, Sodergren M, Kossenkov A, Raulf N, Vasara J, Andrikakou P, Meyer T, Huang K, Plummer R, Chee C, Spalding D, Pai M, Khan S, Pinato D, Sharma R, Basu B, Palmer D, Ma Y, Evans J, Habib R, Martirosyan A, Elasri N Reynaud A, Rossi J, Cobbold M, Habib N, Gabrilovich D2021Clinical Cancer ResearchOncology, Immuno-oncologyMultiplex IHC
Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancerSushentsev N, McLean M, Warren A, Benjamin A, Brodie C, Frary A, Gill A, Jones J, Kaggie J, Lamb B, Locke M, Miller J, Mills I, Priest A, Robb F, Shah N, Schulte R, Graves M, Gnanapragasam V, Brindle K, Barrett T, Gallacher F2022Nature CommunicationsOncologyISH/FISH, Membrane, Multiplex IHC
Antibody-drug conjugate MORAb-202 exhibits long-lasting antitumor efficacy in TNBC PDx modelsFuruuchi K, Rybinski K, Fulmer J, Moriyama T, Drozdowski B, Soto A, Fernando S, Wilson K, Milinichik A, Dula M, Tanaka K, Cheng X, Albone E, Uenaka T2021Cancer ScienceOncologyMultiplex IHC
DS-7300a, a DNA Topoisomerase I Inhibitor, DXd-based Antibody-Drug Conjugate 2 Targeting B7-H3 Exerts Potent Antitumor Activities in Preclinical ModelsYamato M, Hasegawa J, Maejima T, Hattori C, Kumagai K, Watanabe A, Nishiya Y, Shibutani T, Aida T, Hayakawa I, Nakada T, Abe Y, Agatsuma T2022Molecular Cancer TherapeuticsOncologyMultiplex IHC
Prostate cancer androgen receptor activity dictates efficacy of Bipolar Androgen TherapySena L, Kumar R, Sanin D, Thompson E, Rosen D, Dalrymple S, Antony L, Yang Y, Comes-Alexandre C, Hicks J, Jones T, Bowers K, Eskra J, Meyers J, Gupta A, Skaist A, Yegnasubramanian S, Luo J, Brennen W, Kachhap S, Antonarakis E, De Marzo A, Isaacs J, Markowski M, Denmeade S2022medRxivOncologyClassifier, Cytonuclear, Multiplex IHC, Registration
Senescence-Associated Molecules and Tumor-Immune-Interactions as Prognostic Biomarkers in Colorectal CancerKellers F, Fernandez A, Konykiewitz B, Schindeldecker M, Tagscherer K, Heintz A, Jesinghaus M, Roth W, Foersch S2022Frontiers in MedicineOncologyClassifier, Multiplex IHC, Spatial Analysis, Registration, TMA
Tau Pathology in Chronic Traumatic Encephalopathy is Primarily NeuronalButler M, Dixon E, Stein T, Alvarez V, Huber B, Buckland M, McKee A, Cherry J2022Journal of Neuropathology & Experimental NeurologyNeuroscienceMultiplex IHC, Spatial Analysis, Highplex FL
Chemoradiation-induced alteration of programmed death-ligand 1, CD8+ tumor-infiltrating lymphocytes and mucin expression in rectal cancerBaretti M, Zhu Q, Fu W, Meyer J, Wang H, Anders R, Azad N2022OncotargetOncologyMultiplex IHC, Spatial Analysis
Glycosphingolipids are mediators of cancer plasticity through independent signaling pathwaysCumin C, Huang Y, Rossdam C, Ruoff F, Cespedes S, Liang C, Lombardo F, Coelho R, Rimmer N, Konantz M, Lopez M, Alam S, Schmidt A, Calabrese D, Fedier A, Vlajnic T, Itzstein M, Templin M, Buettner F, Everest-Dass A, Heinzelmann-Schwarz V, Jacob F2022Cell reportsOncologyClassifier, Multiplex IHC
Bright-Field Multiplex Immunohistochemistry Assay for Tumor Microenvironment Evaluation in Melanoma TissuesUgolini F, Pasqualini E, Simi S, Baroni G, Massi D2022CancersOtherMultiplex IHC

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