Multiplex IHC
MULTIPLEX IHC
Product Overview
Indica Labs’ Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane) and can be used for a variety of IHC applications. This module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue. Each cell is individually measured for cytoplasmic, nuclear, and/or membrane positivity. The software also reports a variety of other statistics relating size and staining intensity metrics.
In the example above, a tissue core is stained with a brown (DAB) nuclear marker, blue membrane marker, pink/red cytoplasmic marker, and nuclear hematoxylin counterstain. Cells are segmented using a combination of the two nuclear stains (hematoxylin and DAB) and one membrane stain (blue). In the corresponding markup image, positive nuclei are colored brown and negative nuclei colored blue. Similarly, cells identified as positive for the membrane marker are colored blue and cells identified as positive for cytoplasmic marker are pink. Advanced staining courtesy of the Trev and Joyce Deeley Research Centre, BC Cancer Agency.
The Multiplex IHC module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and file formats.
Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.
MULTIPLEX IHC
Indica Labs’ Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane) and can be used for a variety of IHC applications. This module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue. Each cell is individually measured for cytoplasmic, nuclear, and/or membrane positivity. The software also reports a variety of other statistics relating size and staining intensity metrics.
In the example above, a tissue core is stained with a brown (DAB) nuclear marker, blue membrane marker, pink/red cytoplasmic marker, and nuclear hematoxylin counterstain. Cells are segmented using a combination of the two nuclear stains (hematoxylin and DAB) and one membrane stain (blue). In the corresponding markup image, positive nuclei are colored brown and negative nuclei colored blue. Similarly, cells identified as positive for the membrane marker are colored blue and cells identified as positive for cytoplasmic marker are pink. Advanced staining courtesy of the Trev and Joyce Deeley Research Centre, BC Cancer Agency.
Here are a few publications that cite use of our Multiplex IHC module. Your publication not on the list? Drop us an email to let us know about it!
| Title | Authors | Year | Journal | Application | HALO Modules |
|---|---|---|---|---|---|
| Linking epigenetic signature and metabolic phenotype in IDH mutant and IDH wildtype diffuse glioma | Buran Y, Filipski K, Bernatz S, Baumgarten P, Roller B, Zinke J, Zeiner PS, Ilina E, Senft C, Ronellenfitsch MW, Plate KH, Bähr O, Hattingen E, Steinbach JP, Mittelbron M, Harter PN | 2020 | Neuropathology and Applied Neurobiology | Neuroscience | Multiplex IHC, TMA |
| Neoadjuvant immunotherapy leads to pathological responses in MMR-proficient and MMR-deficient early-stage colon cancers | Chalabi M, Fanchi LF, Dijkstra KK, Van den Berg JG, Aalbers AG, Sikorska K, Lopez-Yurda M, Grootscholten C, Beets GL, Snaebjornsson P, Maas M, Mertz J, Veninga V, Bounova G, Broeks A, Beets-Tan RG, de Wijkerslooth TR, van Lent AU, Marsman HA, Nuijten E, Kok NF, Kuiper M, Verbeek WH, Kok M, Van Leerdam ME, Schumacher TN, Voest EE, Haanen JB | 2020 | Nature Medicine | Immuno-oncology | Multiplex IHC, Serial Section |
| Computational image analysis of T-cell infiltrates in resectable gastric cancer: association with survival and molecular subtypes | Challoner BR, von Loga K, Woolston A, Griffiths B, Sivamanoharan N, Semiannikova M, Newey A, Barber LJ, Mansfield D, Hewitt LC, Saito Y, Davarzani N, Starling N, Melcher A, Grabsch HI, Gerlinger M | 2020 | JNCI: Journal of the National Cancer Institute | Immuno-oncology | Multiplex IHC |
| Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary Infection | Desai N, Neyaz A, Szabolcs A, Shih AR, Chen JH, Thapar V, Nieman LT, Solovyov A, Mehta A, Lieb DJ, Kulkarni AS, Jaicks C, Pinto CJ, Juric D, Chebib I, Colvin RB, Kim AY, Monroe R, Warren SE, Danaher P, Reeves JW, Gong J, Rueckert EH, Greenbaum BD, Hacohen N, Lagana SM, Rivera MN, Sholl LM, Stone JR, Ting DT, Deshpande V | 2020 | medRxiv | Infectious Disease | Multiplex IHC |
| PD-L1 Expression and Clinical Outcomes to Cabozantinib, Everolimus, and Sunitinib in Patients with Metastatic Renal Cell Carcinoma: Analysis of the Randomized Clinical Trials METEOR and CABOSUN | Flaifel A, Xie W, Braun DA, Ficial M, Bakouny Z, Nassar AH, Jennings RB, Escudier B, George DJ, Motzer RJ, Morris MJ, Powles, Wang E, Huang Y, Freeman GJ, Choueiri TK, Signoretti S | 2019 | Clinical Cancer Research | Immuno-oncology | Multiplex IHC |
| The effect of cyfluthrin on testis inhibin B in rats and the intervention of Lycium barbarum polysaccharide | Guo X, Xie Y-X, Guo C, Wei J-L, Yang H-F | 2021 | Molecular & Cellular Toxicology | Toxicology | Multiplex IHC |
| Receptor tyrosine kinase expression and phosphorylation in canine nasal carcinoma | Hocker SA, Higginbotham ML, Schermerhorn T, Henningson J | 2017 | Research in Veterinary Science | Oncology | Multiplex IHC |
| Multiplexed Immunohistochemistry for Molecular and Immune Profiling in Lung Cancer‚ÄîJust About Ready for Prime-Time? | Hofman P, Badoual C, Henderson F, Berland L, Hamila M, Long-Mira E, Lassalle S, Roussel H, Hofman V, Tartour E, Ilié M | 2019 | Cancers | Oncology, Immuno-oncology, Review | Classifier, Multiplex IHC |
| FOXM1 contributes to treatment failure in acute myeloid leukemia | Khan I, Halasi M, Patel A, Schultz R, Kalakota N, Chen Y-H, Aardsma N, Liu L, Crispino JD, Mahmud N, Frankfurt O, Gartel AL | 2018 | JCI Insight | Oncology | Multiplex IHC |
| Digital image analysis improves precision of PD‐L1 scoring in cutaneous melanoma | Koelzer VH, Gisler A, Hanhart JC, Griss J, Wagner SN, Willi N, Cathomas G, Sachs M, Kempf W, Thommen DS, Mertz KD | 2018 | Histopathology | Oncology, Immuno-oncology | Classifier, Multiplex IHC |
| Precision immunoprofiling by image analysis and artificial intelligence | Koelzer VH, Sirinukunwattana K, Rittscher J, Mertz KD | 2019 | Vichows Archiv | Review | Classifier, Multiplex IHC, Spatial Analysis |
| The role of WNT10B in normal prostate gland development and prostate cancer | Madueke I, Hu W-Y, Hu D, Swanson SM, Griend DV, Abern M, Prins GS | 2019 | The Prostate | Oncology | Multiplex IHC |
| Immuno-Interface Score to Predict Outcome in Colorectal Cancer Independent of Microsatellite Instability Status | Nestarenkaite A, Fadhil W, Ramusson A, Susanti S, Hadjimichael E, Laurinaviciene A, Ilyas M, Laurinavicius A | 2020 | Cancers | Immuno-oncology | Classifier, Multiplex IHC |
| Two distinct immunopathological profiles in autopsy lungs of COVID-19 | Nienhold R, Ciani Y, Koelzer VH, Tzankov A, Haslbauer JD, Menter T, Schwab N, Henkel M, Frank A, Zsikla V, Willi N, Kempf W, Hoyler T, Barbareschi M, Moch H, Tolnay M, Cathomas G, Demichelis F, Junt T, Mertz KD | 2020 | medRxiv | Immunology, Infectious Disease | Multiplex IHC, HALO AI |
| A single dose of peripherally infused EGFRvIII-directed CAR T cells mediates antigen loss and induces adaptive resistance in patients with recurrent glioblastoma | O’Rourke DM, Nasrallah MP, Desai A, Melenhorst JJ, Mansfield K, MorrissetteJJD, Martinez-Lage M, Brem S, Maloney E, Shen A, Isaacs R, Mohan S, Plesa G, Lacey SF, Navenot J-M, Zheng Z, Levine BL, Okada H, June CH, Brogdon JL, Maus MV | 2017 | Science Translational Medicine | Oncology, Immuno-oncology | Cytonuclear, Multiplex IHC |
| Assessment of associations between clinical and immune microenvironmental factors and tumor mutation burden in resected nonsmall cell lung cancer by applying machine learning to whole-slide images | Ono A, Terada Y, Kawata T, Serizawa M, Isaka M, Kawabata T, Imai T, Mori K, Muramatsu K, Hayashi I, Kenmotsu H, Oshima K, Urakami K, Nagashima T, Kusuhara M, Akiyama Y, Sugino T, Ohde Y, Yamaguchi K, Takahashi T T | 2020 | Cancer Medicine | Classifier, Multiplex IHC, Serial Section | |
| The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis | Oser MG, Sabet AH, Gao W, Chakraborty AA, Schinzel AC, Jennings RB, Fonseca R, Bonal DM, Booker MA, Flaifel A, Novak JS, Christensen VL, Zhang H, Herbert ZT, Tolstorukov MY, Buss EJ, Wong K-K, Bronson RT, Nguyen Q-D, Signoretti S, Kaelin Jr WG | 2019 | Genes & Development | Oncology | Multiplex IHC |
| Immunogradient Indicators for Antitumor Response Assessment by Automated Tumor-Stroma Interface Zone Detection | Rasmusson A, Zilenaite D, Nestarenkaite A, Augulis R, Laurinaviciene A, Ostapenko V, Poskus T, Laurinavicius A | 2020 | The American Journal of Pathology | Multiplex IHC, HALO AI | |
| Physiologic serum 1,25 dihydroxyvitamin D is inversely associated with prostatic Ki67 staining in a diverse sample of radical prostatectomy patients | Rosenberg A, NetteY OS, Gogana P, Sheikh U, Virgilia M, Macias V, Kajdacsy-Balla A, R Sharifi R, Kittles RA, Murphy AB | 2019 | Cancer Causes & Control | Oncology | Multiplex IHC |
| An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression | Sainson RCA, Thotakura AK, Kosmac M, Borhis G, Parveen N, Kimber R, Carvalho J, Henderson SJ, Pryke KL, Okell T, O'Leary S, Ball S, Van Krinks C, Gamand L, Taggart E, Pring EJ, Ali H, Craig H, Wong VWY, Liang Q, Rowlands RJ, Lecointre M, Campbell J, Kirby I, Melvin D, Germaschewski V, Oelmann E, Quaratino S, McCourt M | 2020 | Cancer Immunology Research | Immuno-oncology | Multiplex IHC |
| Nuclear Glycogenolysis Modulates Histone Acetylation in Human Non-Small Cell Lung Cancers | Sun RC, Dukhande VV, Zhou Z, Young LEA, Emanuelle S, Brainson CF, Gentry MS | 2019 | Cell Metabolism | Oncology | Multiplex IHC |
| A transcriptionally and functionally distinct PD-1+ CD8+ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade | Thommen DS, Koelzer VH, Herzig P, Roller A, Trefny M, Dimeloe S, Kiialainen A, Hanhart J, Schil C, Hess C, Prince SS, Wiese M, Lardinois D, Ho P-C, Klein C, Karanikas V, Mertz KD, Schumacher TN, Zippelius A | 2018 | Nature Medicine | Oncology, Immuno-oncology | Multiplex IHC |
| Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters | Tostanoski LH, Wegmann F, Martinot AJ, Loos C, McMahan K, Mercado NB, Yu J, Chan CN, Bondoc S, Starke CE, Nekorchuk M, Busman-Sahay K, Piedra-Mora C, Wrijil LM, Ducat S, Custers J, Atyeo C, Fischinger S, Burke JS, Feldman J, Hauser BM, Caradonna TM, Bondzie EA, Dagotto G, Gebre MS, Jacob-Dolan C, Lin Z, Mahrokhian SH, Nampanya F, Nityanandam R, Pessaint L, Porto M, ALi V, Benetiene D, Tevi K, Andersen H, Lewis MG, Schmidt AG, Lauffenburger DA, Alter G, Estes JD, Schuitemaker H, Zahn R, Barouch DH | 2020 | Nature Medicine | Infectious Disease | Area Quantification, Multiplex IHC |
| Inducing and exploiting vulnerabilities for the treatment of liver cancer | Wang C, Vegna S, Jin H, Benedict B, Lieftink C, Ramirez C, de Oliveira RL, Morris B, Gadiot J, Wang W, du Chatinier A, Wang L, Gao D, Evers B, Jin G, Xue Z, Schepers A, Jochems F, Sanchez AM, Mainardi S, te Riele H, Beijersbergen RL, Qin W, Akkari L, Bernards R | 2019 | Nature | Oncology | Multiplex IHC |
| Digital Immunophenotyping Predicts Disease Free and Overall Survival in Early Stage Melanoma Patients | De Logu F, Galli F, Nassini R, Ugolini F, Simi S, Cossa M, Miracco C, Gianatti A, De Giorgi V, Rulli E, Cossu A, Massi D, Mandalà M | 2021 | Cells | Oncology, Immuno-oncology | Multiplex IHC |
| Activated Regulatory T-Cells, Dysfunctional and SenescentT-Cells Hinder the Immunity in Pancreatic Cancer | Sivakumar S, Abu-Shah E, Ahern DJ, Arbe-Barnes EH, Jainarayanan AK, Mangal N, Reddy S, Rendek A, Easton A, Kurz E, Silva M, Soonawalla Z, Heij LR, Bashford-Rogers R, Middleton MR, DUstin ML | 2021 | cancers | Classifier, Multiplex IHC, Spatial Analysis, Highplex FL | |
| Age-associated expression of p21and p53 during human wound healing | Chia CW, Sherman-Baust CA, Larson SA, Pandey R, Withers R, Karikkineth AC, Zukley LM, Campisi J, Egan JM, Sen R, Ferrucci L | 2021 | Aging Cell | Dermatology | Area Quantification, ISH/FISH, Multiplex IHC |
| Inhibition of STAT3 augments antitumor efficacy of anti-CTLA-4 treatment against prostate cancer | Witt K, Evans-Axelsson S, Lundqvist A, Johansson M, Bjartell A, Hellsten R | 2021 | Cancer Immunology, Immunotherapy | Immuno-oncology | Area Quantification, Multiplex IHC |
| Tumor cell PD-L1 expression is a strong predictor of unfavorable prognosis in immune checkpoint therapy-naive clear cell renal cell cancer | Möller K, Fraune C, Blessin NC, Lennartz M, Kluth M, Hube-Magg C, Lindhorst L, Dahlem R, Fisch M, Eichenauer T, Riechardt S, Simon R, Sauter G, Büscheck F, Höppner W, Matthies C, Doh O, Krech T, Marx AH, Zecha H, Rink M, Steurer S, Clauditz TS | 2021 | International Urology and Nephrology | Immuno-oncology | Multiplex IHC, TMA |
| TNFα secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapy | Wei Z, Singh O, Ekinci C, Gill J, Li M, Mamatjan Y, Karimi S, Bunda S, Mansouri S, Aldape K, Zadeh G | 2021 | Acta Neuropathologica Communications | Immunology, Neuroscience | Classifier, Multiplex IHC |
| Mitigation of endemic GI-tract pathogen-mediated inflammation through development of multimodal treatment regimen and its impact on SIV acquisition in rhesus macaques | Bochart RM, Busman-Sahay K, Bondoc S, Morrow DW, Ortiz AM, Fennessey CM, Fischer MB, Shiel O, Swanson T, Shriver-Munsch CM, Crang HB, Armantrout KM, Barber-Axthelm AM, Langner C, Moats CR, Labriola CS, MacAllister R, Axthelm MK, Brenchley JM, Keele BF, Estes JD, Hansen SG, Smedley JV | 2021 | PLOS Pathogens | Infectious Disease | ISH/FISH, Multiplex IHC |
| Analysis of genomic and non-genomic signaling of estrogen receptor in PDX models of breast cancer treated with a combination of the PI3K inhibitor alpelisib (BYL719) and fulvestrant | Jacquemetton J, Kassem L, Poulard C, Dahmani A, De Plater L, Montaudon E, Sourd L, Morisset L, El Botty R, Chateau-Joubert S, Vacher S, Bièche I, Treilleux I, Trédan O, Marangoni E, Le Romancer M | 2021 | Breast Cancer Research | Oncology | Multiplex IHC |
| Pathways of immune exclusion in metastatic osteosarcoma are associated with inferior patient outcomes | Ligon JA, Choi W, Cojocaru G, Fu W, Hsiue EH-C, Oke TF, Siegel N, Fong MH, Ladle B, Pratilas CA, Morris CD, Levin A, Rhee DS, Meyer CF, Tam AJ, Blosser R, Thompson ED, Suru A, McConkey D, Housseau F, Anders R, Pardoll DM, Llosa N | 2021 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Multiplex IHC |
| Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine release | Dang K, Castello G, Clarke S, et al. | 2021 | Journal for Immunotherapy of Cancer | Immuno-oncology | Multiplex IHC |
| Multiparametric Analyses of Hepatocellular Carcinoma Somatic Mouse Models and Their Associated Tumor Microenvironment | Taranto D, Ramirez C, Vegna S, et al. | 2021 | Current Protocols | Oncology | Multiplex IHC |
| Rejection of benign melanocytic nevi by nevus-resident CD4+ T cells | Schiferle E, Cheon S, Ham S, Son H, Messerschmidt J, Lawrence D, Cohen J, Flaherty K, Moon J, Lian C, Sullivan R, Demehri S | 2021 | Science Advances | Oncology | Multiplex IHC |
| Extensive Three-Dimensional Intratumor Proteomic Heterogeneity Revealed by Multiregion Sampling in High-Grade Serous Ovarian Tumor Specimens | Hunt A, Bateman N, Barakat W, Makohon-Moore S, Hood B, Conrads K, Zhou M, Calvert V, Pierobon M, Loffredo J, Litzi T, Oliver J, Mitchell D, Gist G, Rojas C, Blanton B, Robinson E, Odunsi K, Sood A, Casablanca Y, Darcy K, Schriver C, Petricoin E, Rao U, Maxwell G, Conrads T | 2021 | iScience | Multiplex IHC | |
| TMEM229A suppresses non?small cell lung cancer progression via inactivating the ERK pathway | Zhang X, He Y, Jiang Y, Bao Y, Chen Q, Xie D, Yu H, Wang X | 2021 | Oncology Reports | Multiplex IHC | |
| Glucocorticoid receptor antagonism promotes apoptosis in solid tumor cells | Greenstein A, Hunt H | 2021 | Oncotarget | Multiplex IHC | |
| A single-dose mRNA vaccine provides a long-term protection for hACE2 transgenic mice from SARS CoV-2 | Huang Q, Ji K, Tian S, Wang F, Huang B, Tong Z, Tan S, Hao J, Wang Q, Tan W, Gao G, Yan J | 2021 | Nature Communications | Infectious Disease | Multiplex IHC |
| ACT1 is required for murine IL-23-induced psoriasiform inflammation potentially independent of E3 ligase activity | Lipovsky A, Slivka PF, Su Z, Wang Y, Paulsboe S, Wetter J, Namovic MT, Gauvin D, Perron D, Gauld S, McGaragughty S, Goedken ER | 2021 | Journal of Investigative Dermatology | Immunology | Multiplex IHC |
| Pharmacologic modulation of RNA splicing enhances anti-tumor immunity | Lu S, De Neef E, Thomas J, Sabio E, Rousseau B, Gigoux M, Knorr D, Greenbaum B, Elhanti Y, Hogg S, Chow A, Ghosh A, Xie A, Zamarin D, Cui D, Erickson C, Singer M, Cho H, Wang E, Lu B, Durham B, Shah H, Chowell D, Gabel A, Shen Y, Liu J, Jin J, Rhodes M, Taylor R, Molina H, Wolchok J, Merghoub T, Diaz Jr. L, Abdel-Wahab O, Bradley B | 2021 | Cell | Oncology | Multiplex IHC |
| Olaparib and durvalumab in patients with germline BRCA-mutated metastatic breast cancer (MEDIOLA): an open-label, multicentre, phase 1/2, basket study | Domchek SM, Postel-Vinay S, Im S-A, Park YH, Delord J-P, Italiano A, Alexandre J, You B, Bastian S, Krebs MG, Wang D, Waqar SN, Lanasa M, Rhee J, Gao H, Rocher-Ros V, Jones EV, Gulati S, Coenen-Stass A, Kozarewa I, Lai Z, Angell HK, Opincar L, Herbolsheimer P, Kaufman B | 2020 | The Lancet Oncology | Oncology | Multiplex IHC |
| ARID1A influences HDAC1/BRD4 activity, intrinsic proliferative capacity and breast cancer treatment response | Nagarajan S, Rao SV, Sutton J, Cheeseman D, Dunn S, Papachristou EK, Prada J-EG, Couturier D-L, Kumar S, Kishore K, Chilamakuri CSR, Glont E, Goode EA, Brodie C, Guppy N, Natrajan R, Bruna A, Caldas C, Russel A, Siersbaek R, Yusa K, Chernukhin I, Carroll JS | 2020 | Nature Genetics | Oncology | Multiplex IHC |
| Multimodal treatment combining cold atmospheric plasma and acidic fibroblast growth factor for multi?tissue regeneration | Tan F, Rui X, Xiang X, Yu Z, Al-Rubeai M | 2021 | The FASEB Journal | Other | Multiplex IHC |
| ILC2-driven innate immune checkpoint mechanism antagonizes NK cell antimetastatic function in the lung | Schuijs MJ, Png S, Richard AC, Tsyben A, Hamm G, Stockis J, Garcia C, Panaud S, Nicholls A, Ros XR, Su J, Eldridge MD, Riedel A, Serrao EM, Rodewald H-R, Mack M, Shields JD, Cohen ES, McKenzie ANJ, Goodwin RJA, Brindle KM, Marioni JC, Halim TYF | 2020 | Nature Immunology | Immuno-oncology | Multiplex IHC |
| Imaging Tumor-Infiltrating Lymphocytes in Brain Tumors with [64Cu]Cu-NOTA-anti-CD8-Positron Emission Tomography | Nagle V, Henry K, Hertz C, et al. | 2021 | Clinical Cancer Research | Immuno-oncology | Multiplex IHC |
| MAOA-mediated reprogramming of stromal fibroblasts promotes prostate tumorigenesis and cancer stemness | Li J, Pu T, Yin L, Li Q, Liao C-P, Wu BJ | 2020 | Oncogene | Oncology | Multiplex IHC |
| Intraductal pancreatic cancer is less responsive than cancer in the stroma to neoadjuvant chemotherapy | Fujikura K, Hutchings D, Braxton AM, Zhu Q, Laheru DA, Hruban RH, Thompson ED, Wood LD | 2020 | Modern Pathology | Oncology | Multiplex IHC |
| Development of a clinically relevant ovarian cancer model incorporating surgical cytoreduction to evaluate treatment of micro-metastatic disease | Morse CB, Voillet V, Bates BM, Chiu EY, Garcia NM, Gottardo R, Greenberg PD, Anderson KG | 2020 | Gynecologic Oncology | Oncology | Multiplex IHC |
| Necroptosis in biliary atresia of the liver | Hashimoto M, Fujishima F, Lomphithak T, Jitkaew S, Nio M, Sasano H | 2021 | Medical Molecular Morphology | Metabolism | Multiplex IHC |
| Baricitinib Treatment Resolves Lower-Airway Macrophage Inflammation and Neutrophil Recruitment in Sars-Cov-2-Infected Rhesus Macaques | Hoang T, Pino M, Boddapati A, Viox E, Starke C, Upadhyay A, Gumber S, Nekorchuk M, Busman-Sahay K, Strongin Z, Harper J, Tharp G, Pellegrini K, Kirejczyk S, Zandi K, Tao S, Horton T, Beagle E, Mahar E, Lee M, Cohen J, Jean S, Wood J, Connor-Stroud F, Stammen R, Delmas O, Wang S, Cooney K, Sayegh M, Wang L, Filev P, Weiskopf D, Silvestri G, Waggoner J, Piantadosi A, Kasturi S, Al-Shakhshir H, Ribeiro S, Sekaly R, Levit R, Estes J, Vanderfold T, Schinazi R, Bosinger S, Paiardine M | 2021 | Cell | Infectious Disease | Multiplex IHC |
| Large-Section Histopathology Can Better Indicate the Immune Microenvironment and Predict the Prognosis of Pancreatic Ductal Adenocarcinoma Than Small-Section Histopathology | Ding G, Guo M, Yang Y, Sun C, Wu S, Liu X, Wang J, Jiang H, Liu Y, Zheng J | 2021 | Frontiers in Oncology | Oncology | Multiplex IHC |
| Suppression of insulin-induced gene 1 (INSIG1) function promotes hepatic lipid remodelling and restrains NASH progression | Azzu V, Vacca M, Kamzolas I, Hall Z, Leslie J, Carobbio S, Virtue S, Davies S, Lukasik A, Dale M, Bohlooly-Y M, Acharjee A, Linden D, Bidault G, Petsalaki E, Griffin J, Oakley F, Allison M, Vidal-Puig | 2021 | Molecular Metabolism | Metabolism | Area Quantification, Cytonuclear, Multiplex IHC |
| Inter-organ transmission of hepatocellular senescence induces multi-organ dysfunction through the TGF? signalling pathway | Kiourtis C, Terradas-Terradas M, Gee L, Hsieh Y, Nixon C, Clark W, Shaw R, Hanson P, Sumpton D, Mackay G, May S, Muller M, Najumudeen A, Campbell A, Barry S, Morris C, LeBeau F, Sansom O, Kirschner K, Oakley F, Bird T | 2021 | Research Square | Other | Area Quantification, Cytonuclear, Multiplex IHC |
| IL6/STAT3 Signaling Hijacks Estrogen Receptor ? Enhancers to Drive Breast Cancer Metastasis | Siersbæk R, Scabia V, Nagarajan S, Chernukhin I, Papachristou EK, Broome R, Johnston SJ, Joosten SEP, Green AR, Kumar S, Jones J, Omarjee S, Alvarez-Fernandez R, Glont S, Aitken SJ, Kishore K, Cheeseman D, Rakha EA, D'Santos C, Zwart W, Ruseel A, Brisken C, Carroll JS | 2020 | Cancer Cell | Oncology | Area Quantification, Multiplex IHC |
| Over-expression of Th2-related molecules in the skin of patients with eosinophilic dermatosis of hematologic malignancy | Maglie R, Ugolini F, De Logu F, Nassini R, Simi S, Nardiello P, Pasqualaini E, Baroini G, Del Bianco E, Massi D, Antiga E | 2021 | Journal of the American Academy of Dermatology | Oncology, Immuno-oncology | Area Quantification, Multiplex IHC |
| The RAC1 target NCKAP1 plays a crucial role in progression of BRAF/PTEN -driven melanoma in mice | Swaminathan K, Campbell A, Papalazarou V, Jaber-Hijazi F, Nixon C, McGhee E, Strathdee D, Sansom OJ, Machesky LM | 2020 | Journal of Investigative Dermatology | Oncology, Dermatology | Area Quantification, Multiplex IHC |
| The TRPA1 Channel Amplifies the Oxidative Stress Signal in Melanoma | De Logu F, Monteiro de Araujo D, Ugolini F, Iannone L, Vannucchi M, Portelli F, Landini L, Titiz M, De Giorgi V, Geppetti P, Massi D, Nassini R | 2021 | Cells | Area Quantification, Multiplex IHC, HALO AI |
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| p53-mediated redox control promotes liver regeneration and maintains liver function in response to CCl4 | Humpton T, Hall H, Kiourtis C, Nixon C, Clark W, Hedley A, Shaw R, Bird T, Blyth K, Vousden K | 2021 | Cell Death & Differentiation | Metabolism | Cytonuclear, Multiplex IHC |
| Combined CTLA-4 and PD-L1 blockade in patients with chemotherapy-naïve metastatic castration-resistant prostate cancer is associated with increased myeloid and neutrophil immune subsets in the bone microenvironment | Subudhi S, Siddiqui B, Aparicio A, Yadav S, Basu S, Chen H, Jindal S, Tidwell R, Varma A, Logothetis C, Allison J, Corn P, Sharma P | 2021 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Cytonuclear, Multiplex IHC |
| Development of a novel humanized mouse model for improved evaluation of in vivo anti-cancer effects of anti-PD-1 antibody | Katano I, Hanazawa A, Otsuka I, Yamaguchi T, Mochizuki M, Kawai K, Ito R, Goto M, Kagawa T, Takahashi T | 2021 | Scientific Reports | Oncology | Cytonuclear, Multiplex IHC |
| A comparability study of natural and deglycosylated PD-L1 levels in lung cancer: evidence from immunohistochemical analysis | Mei J, Xu J, Yang X, Gu D, Zhou W, Wang H, Liu C | 2021 | Molecular Cancer | Oncology | Cytonuclear, Multiplex IHC |
| A faithful in vivo model of human macrophages in metastatic melanoma | Voillet V, Berger T, McKenna K, Paulson K, Smythe K, Hunter D, Valente W, Weaver S, Campbell J, Kim T, Byrd D, Bielas J, Pierce R, Chapuis A, Gottardo R, Rongvaux | 2021 | bioRxiv | Cytonuclear, Multiplex IHC | |
| E3 ligase-inactivation rewires CBL interactome to elicit oncogenesis by hijacking RTK–CBL–CIN85 axis | Ahmed S, Buetow L, Gabrielsen M, Lilla S, Sibbet G, Sumpton D, Zanivan S, Hedley A, Clark W, Huang D | 2021 | Oncogene | Oncology | Cytonuclear, Multiplex IHC |
| CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation | Le A, Yelland T, Paul N, Fort L, Nikolaou S, Ismail S, Machesky L | 2021 | Journal of Cell Biology | Other | Cytonuclear, Multiplex IHC |
| In vitro amplification of pathogenic tau conserves disease-specific bioactive characteristics | Xu H, O'Reilly M, Gibbons G, Changolkar L, McBride J, Riddle D, Zhang B, Stieber A, Nirschl J, Kim S, Hoxha K, Brunden K, Schellenberg G, Trojanowski J, Lee V | 2021 | Acta Neuropathologica | Neruoscience | Cytonuclear, Multiplex IHC |
| CCNE1 amplification is synthetic-lethal with PKMYT1 kinase inhibition | Gallo D, Young J, Fourtounis J, Martino G, Alvarez-Quilon A, Bernier C, Duffy N, Papp R, Roulston A, Stocco R, Szychowski J, Veloso A, Alam H, Baruah P, Fortin A, Bowlan J, Chaudhary N, Desjardins J, Dietrich E, Fournier S, Fugere-Desjardins C, de Rugy T, Leclaire M, Liu B, Melo H, Nicolas O, Singhania A, Szilard R, Tkac J, Yin S, Morris S, Zinda M, Marshall C, Duocher D | 2021 | bioRxiv | Other | Cytonuclear, Multiplex IHC |
| Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicity | Qiao Y, Qiu Y, Ding J, Luo N, Wang H, Ling X, Sun J, Wu Z, Wang Y, Liu Y, Guo F, Sun T, Shen W, Zhang M, Wu D, Chen B, Xu W, Wang X | 2021 | Nature Communications | Immuno-oncology | Cytonuclear, Multiplex IHC |
| Pixelwise H-score: a novel digital image analysis-based metric to quantify membrane biomarker expression from immunohistochemistry images | Ram S, Vizcarra P, Whalen P, Deng S, Painter C, Jackson-Fisher A, Pirie-Shepherd S, Xia X, Powell E | 2021 | PLOS One | Other | Cytonuclear, Multiplex IHC |
| LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis | Wei W, Wang N, Deng M, Dong P, Liu J, Xiang Z, Li X, Li Z, Liu Z, Peng Y, Li Z, Jiang L, Yao K, Ye Y, Lu W, Zhang Z, Zhou F, Liu Z, Xie D, Yu C | 2021 | Redox Biology | Metabolism | Cytonuclear, Multiplex IHC |
| Hedgehog-signalling in papillary fibroblasts is essential for hair follicle regeneration during wound healing | Frech S, Forsthuber A, Korosec A, Lipp K, Kozumov V, Lichtenberger B | 2021 | Journal of Investigative Dermatology | Dermatology | Cytonuclear, Multiplex IHC |
| Evaluation of Early Markers of Ischemia-reperfusion Injury and Preservation Solutions in a Modified Hindlimb Model of Vascularized Composite Allotransplantation | Rostami S, Xu M, Datta S, Haykal S | 2022 | Transplantation Direct | Cytonuclear, Multiplex IHC | |
| B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinoma | Yamada T, Miki Y, Suzuki M, Kondoh O, Saito-Koyama R, Ono K, Okada Y, Sasano H | 2021 | Cancer Medicine | Oncology | Cytonuclear, Multiplex IHC |
| Discoidin Domain Receptor 1 (DDR1) is Necessary for Tissue Homeostasis in Pancreatic Injury and Pathogenesis of Pancreatic Ductal Adenocarcinoma | Ruggeri JM, Franco-Barraza J, Sohail A, Zhang Y, Long D, di Magliano MP, Cukierman E, Fridman R, Crawford HC | 2020 | The American Journal of Pathology | Oncology, Immunology | Cytonuclear, Multiplex IHC, Highplex FL |
| CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta, alpha, beta, and gamma | Geng J, Chen L, Yuan Y, Wang K, Wang Y, Qin C, Wu G, Chen R, Zhang Z, Wei D, Du P, Zhang J, Lin P, Zhang K, Deng Y, Xu K, Liu J, Sun X, Guo T, Yang X, Wu J, Jiang J, Li L, Zhang K, Wang Z, Zhang J, Yan Q, Zhu H, Zheng Z, Miao J, Fu X, Yang F, Chen X, Tang H, Zhang Y, Shi Y, Zhu Y, Pei Z, Huo F, Liang X, Wang Y, Wang Q, Xie W, Li Y, Shi M, Bian H, Zhu P, Chen Z | 2021 | Signal Transduction and Targeted Therapy | Infectious Disease | Cytonuclear, Multiplex IHC, Highplex FL |
| Cross-linking of T cell to B cell lymphoma by the T cell bispecific antibody CD20-TCB induces IFN?/CXCL10-dependent peripheral T cell recruitment in humanized murine model | Cremasco F, Menietti E, Speziale D, Sam J, Sammicheli S, Richard M, Varol A, Klein C, Umana P, Bacac M, Colombetti S, Perro M | 2021 | PLOS ONE | Oncology | Cytonuclear, Multiplex IHC, Highplex FL |
| mRNA translation is a therapeutic vulnerability necessary for bladder epithelial transformation | Jana S, Deo R, Hough R, et al. | 2021 | JCI Insight | Oncology | Multiplex IHC, Highplex FL |
| Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade | Srivastava S, Furlan SN, Jaeger-Ruckstuhl CA, Sarvothama M, Berger C, Smythe KS, Garrison SM, Specht JM, Lee SM, Amezquita RA, Voillet V, Muhunthan V, Yechan-Gunja S, Pillai SPS, Rader C, Houghton AM, Pierce RH, Gottardo R, Maloney DG, Riddell SR | 2020 | Cancer Cell | Immuno-oncology | Multiplex IHC, Highplex FL |
| STAT3 antisense oligonucleotide remodels the suppressive tumor microenvironment to enhance immune activation in combination with anti-PD-L1 | Proia T, Singh M, Woessner R, Carnevalli LS, Bommakanti G, Magiera L, Srinivasan SS, Grosskurth SE, Collins M, Womack C, Griffin M, Ye M, Cantin S, Russell DL, Xie M, Hughes AM, Deng N, Mele DA, Fawell SE, Barry ST, Reimer C, Barrett JC, McCoon P | 2020 | Clinical Cancer Research | Immuno-oncology | Multiplex IHC, Highplex FL |
| Neonatal hydrocephalus leads to white matter neuroinflammation and injury in the corpus callosum of Ccdc39 hydrocephalic mice | Goulding DS, Vogel RC, Pandya CD, Shula C, Gensel JC, Mangano FT, Goto J, Miller BA | 2020 | Journal of Neurosurgery | Immunology, Neuroscience | Multiplex IHC, Highplex FL |
| Immune evasion before tumour invasion in early lung squamous carcinogenesis | Mascaux C, Angelova M, Vasaturo A, Beane J, Hijazi K, Anthoine G, Rothe F, Willard-Gallo K, Haller A, Ninane V, Burny A, Sculier J-P, Spira A, Galon J | 2019 | Nature | Oncology, Immuno-oncology | Multiplex IHC |
| Cooperative targeting of immunotherapy-resistant melanoma and lung cancer by an AXL-targeting antibody-drug conjugate and immune checkpoint blockade | Boshuizen J, Pencheva N, Krijgsman O, Altimari DD, Castro PG, de Bruijn B, Ligtenberg MA, den Heuvel EG, Vredevoogd DW, Song J-Y, Visser N, Apriamashvili G, Janmaat ML, Plantinga TS, Franken P, Houtkamp M, Lingnau A, Jure-Kunkel M, Peeper DS | 2021 | Cancer Research | Immuno-oncology | Multiplex IHC |
| Intratumoral Heterogeneity and Immune Response Indicators to Predict Overall Survival in a Retrospective Study of HER2-Borderline (IHC 2+) Breast Cancer Patients | Radziuviene G, Rasmusson A, Augulis R, Grineviciute R, Zilenaite D, Laurinaviciene A, Ostapenko V, Laurinavicius A | 2021 | Frontiers in Oncology | Immuno-oncology | Membrane, Multiplex IHC, HALO AI |
| Two distinct immunopathological profiles in autopsy lungs of COVID-19 | Nienhold R, Ciani Y, Koelzer V, Tzankov A, Haslbauer J, Menter T, Schwab N, Henkel M, Frank A, Zsikla V, Willi N, Kempf W, Hoyler T, Barbareschi M, Moch H, Tolnay M, Cathomas G, Dernichelis F, Junt T, Mertz K | 2021 | Nature Communications | Infectious Disease | Multiplex IHC, HALO AI |
| Tertiary lymphoid structures critical for prognosis in endometrial cancer patients - a TransPORTEC study | Horeweg N, Workel, H, Loiero D, Church D, Vermij L, Léon-Castillo A, Krog R, de Boer S, Nout R, Powel M, Mileshkin L, MacKay H, Leary A, Singh N, Jürgenliemk-Schulz I, Creutzberg C, Koelzer V, Nijman H, Bosse T, Bruyn M | 2021 | Research Square | Multiplex IHC, HALO AI, TMA | |
| Endothelial cell infection and dysfunction, immune activation in severe COVID-19 | Qin Z, Liu F, Blair R, Wang C, Yang H, Mudd J, Currey J, Iwanaga N, He J, Mi R, Han K, Midkiff C, Alam M, Aktas B, Heide R, Veazey R, Piedimonte G, Maness N, Ergün S, Mauvais-Jarvis F, Rappaport J, Kolls J, Qin X | 2021 | Theranostics | Immunology, Infectious Disease | Multiplex IHC, Spatial Analysis |
| Establishing standardized immune phenotyping of metastatic melanoma by digital pathology | Sobottka B, Nowak M, Frei A, Haberecker M, Merki S, Tumor Profiler consortium, Levesque M, Dummer R, Moch H, Koelzer V | 2021 | Laboratory Investigation | Immuno-oncology | Multiplex IHC, Spatial Analysis, HALO AI |
| Genomic analysis of Vascular Invasion in Hepatocellular Carcinoma (HCC) Reveals Molecular Drivers and Predictive Biomarkers | Krishnan MS, Rajan KD A, Park J, Arjunan V, Marques FJG, Bermudez A, Girvan OA, Hoang NS, Yin J, Nguyen MH, Kothary N, Pitteri S, Felsher DW, Dhanasekaran R | 2020 | Hepatology | Oncology | Multiplex IHC, TMA |
| B7-H3 immune checkpoint expression is a poor prognostic factor in colorectal carcinoma | Lu Z, Zhao Z-X, Cheng P, Huang F, Guan X, Zhang M-G, Chen H-P, Liu Z, Jiang Z, Zheng Z-X, Zou S-M, Wang X-S | 2020 | Modern Pathology | Immuno-oncology | Multiplex IHC, TMA |
| A preclinical trial and molecularly-annotated patient cohort identify predictive biomarkers in homologous recombination deficient pancreatic cancer | Wang Y, Park J, Pacis A, Denroche R, Jang G, Zhang A, Cuggia A, Domecq C, Monlong J, Raitses-Gurevich M, Grant R, Borgida A, Holter S, Stossel C, Bu S, Masoomian M, Lungu I, Bartlett J, Wilson J, Gao Z, Riazalhosseini Y, Asselah J, Bouganim N, Cabrera T, Boucher L, Valenti D, Biagi J, Greenwood C, Polak P, Foulkes W, Golan T, O'Kane G, Fischer S, Knox J, Gallinger S, Zogopoulos G | 2020 | Clinical Cancer Research | Oncology | Classifier, Cytonuclear, Multiplex IHC |
| Glucagon Receptor Inhibition Reduces Hyperammonemia and Lethality in Male Mice with Urea Cycle Disorder | Cavino K, Sung B, Su Q, Na E, Kim J, Cheng X, Gromada J, Okamoto H | 2020 | Endocrinology | Metabolism | ISH/FISH, Multiplex IHC |
| Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effects | Escuin-Ordinas H, Liu Y, Hufo W, Dimatteo R, Huang R, Krystofinski P, Azhdam A, Lee J, Comin-Anduix B, Cochran A, Lo R, Segura T, Scumpia P, Ribas A | 2021 | PLOS ONE | Dermatology | Object Colocalization, Multiplex IHC |
| Specificity and off-target effects of AAV8-TBG viral vectors for the manipulation of hepatocellular gene expression in mice | Kiourtis C, Wilczynska A, Nixon C, Clark W, May S, Bird T | 2021 | Biology Open | Other | Multiplex IHC |
| Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivation | Wu H, He P, Ren Y, Xiao S, Wang W, Liu Z, Li H, Wang Z, Zhang D, Cai J, Zhou X, Jiang D, Fei X, Zhao L, Zhang H, Liu Z, Chen R, Li W, Wang C, Zhang S, Qin J, Nasan B, Sun C | 2022 | Nature Communications | Infectious Disease | Multiplex IHC |
| Transcription factor Mesenchyme Homeobox Protein 2 (MEOX2) modulates nociceptor function | Kokotovic T, Lenartowicz E, Langeslag M, Ciotu C, Fell C, Scaramuzza A, Fischer M, Kress M, Penninger J, Nagy V | 2022 | The FEBS Journal | Neruoscience | Multiplex IHC |
| The microglial P2Y6 receptor mediates neuronal loss and memory deficits in neurodegeneration | Puigdellivol M, Milde S, Vilalta A, Cockram T, Allendorf D, Lee J, Dundee J, Pampuscenko K, Borutaite V, Nuthall H, Brelstaff J, Spillantini G, Brown G | 2021 | Cell Reports | Neuroscience | Area Quantification, Cytonuclear, Multiplex IHC |
| An Fc-inert PD-L1×4-1BB bispecific antibody mediates potent anti-tumor immunity in mice by combining checkpoint inhibition and conditional 4-1BB co-stimulation | Muik A, Altintas I, Gieseke F, Schoedel K, Burm S, Toker A, Salcedo T, Verzijl, Eisel D, Grunwitz C, Kranz L, Vormehr M, Satijn D, Diken M, Kreiter S, Sasser K, Ahmadi T, Tureci O, Breij E, Jure-Kunkel M, Sahin U | 2022 | OncoImmunology | Immuno-oncology | Area Quantification, Immune, Multiplex IHC |
| An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancer | Voabil P, de Bruijn M, Roelofsen L, Hendriks S, Brokamp S, van den Braber M, Broeks A, Sanders J, Herzig P, Zippelius A, Blank C, Hartemink K, Monkhorst K, Haanen J, Schumacher T, Thommen D | 2021 | Nature Medicine | Immuno-oncology | Classifier, Multiplex IHC |
| Investigation of Breast Cancer Microstructure and Microvasculature from Time-Dependent DWI and CEST in Correlation with Histological Biomarkers | Someya Y, Iima M, Imai H, Yoshizawa A, Kataoka M, Isoda H, Le Bihan D, Nakamoto Y | 2021 | Research Square | Classifier, Multiplex IHC | |
| A selective and orally bioavailable VHL-recruiting PROTAC achieves SMARCA2 degradation in vivo | Kofink C, Trainor N, Mair B, Wrhrle S, Wurm M, Mischerikow N, Bader G, Rumpel K, Gerstberger T, Cui Y, Greb P, Garavel G, Scharnweber M, Fuchs J, Gremel G, Chette P, Hopf S, Budano N, Rinnenthal J, Gmaschitz G, Diers E, McLennan R, Roy M, Whitworth C, Vetma V, Mayer M, Koegl M, Ciulli A, Weinstabl H, Farnaby W | 2022 | Biological and Medicinal Chemistry | Classifier, Cytonuclear, Multiplex IHC | |
| Pancreas resident macrophage-induced fibrosis has divergent roles in pancreas inflammatory injury and PDAC | Baer J, Zuo C, Kang L, Alarcon de la Lastra A, Bocherding N, Knolhoff B, Bogner S, Zhu Y, Lewis M, Zhang N, Kim K, Fields R, Mills J, Ding L, Randolph G, DeNardo D | 2022 | bioRxiv | Fibrosis | Classifier, Area Quantification, Cytonuclear, Multiplex IHC, Highplex FL, Figure Maker |
| Anti-CAIX BB? CAR4/8 T Cells Exhibit Superior Efficacy in a Clear Cell Renal Cell Carcinoma (ccRCC) Mouse Model | Wang Y, Buck A, Grimaud M, Culhane A, Kodangattil S, Razimbaud C, Bonal D, Nguyen Q, Zhu Z, Wei K, O'Donnell M, Huang Y, Signoretti S, Choueiri T, Freeman G, Zhu Q, Marasco | 2021 | Molecular Therapy: Oncolytics | Oncology | Cytonuclear, Multiplex IHC |
| Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer | Zheng S, Zou Y, Tang Y, Yang A, Liang J, Wu L, Tian W, Xiao W, Xie X, Yang L, Xie J, Wei W, Xie X | 2022 | OncoImmunology | Immuno-oncology | Cytonuclear, Multiplex IHC |
| Collaborative workflow between pathologists and deep learning for evaluation of tumor cellularity in lung adenocarcinoma | Sakamoto T, Furukawa T, Pham H, Kuroda K, Tabata K, Kshima Y, Okoshi E, Morimoti S, Bychkov A, Fukuoka J | 2022 | bioRxiv | Oncology | Cytonuclear, Multiplex IHC |
| Pathogenesis of follicular thymic hyperplasia associated with rheumatoid arthritis | Ohe R, Yang S, Yamashita D, Ichikawa C, Saito A, Kabasawa T, Utsunomiya A, Aung N, Urano Y, Kitaoka T, Suzuki K, Takahara D, Sasaki A, Takakubo Y, Takagi M, Yamakawa M, Futakuchi M | 2022 | Pathology International | Other | Cytonuclear, Multiplex IHC |
| Nuclear expression of pSTAT3Tyr705 and pSTAT3Ser727 in the stromal compartment of localized hormone-naïve prostate cancer | Marginean F, Hellsten R, Krzyanowska A, Bjartell A | 2022 | Pathology - Research and Practice | Oncology | Cytonuclear, Multiplex IHC, TMA |
| Tertiary lymphoid structures critical for prognosis in endometrial cancer patients - a TransPORTEC study | Horeweg N, Workel, H, Loiero D, Church D, Vermij L, Léon-Castillo A, Krog R, de Boer S, Nout R, Powel M, Mileshkin L, MacKay H, Leary A, Singh N, Jürgenliemk-Schulz I, Creutzberg C, Koelzer V, Nijman H, Bosse T, Bruyn M | 2022 | Nature Communications | Oncology | Cytonuclear, Multiplex IHC, TMA |
| ZEB1 transcription factor promotes immune escape in melanoma | Plaschka M, Benboubker V, Grimont M, Berthet J, Tonon L, Lopez J, Le-Bouar M, Balme B, Tondeau G, de la Fouchardiere A, Larue L, Puisieux A, Grinberg-Bleyer Y, Bendriss-Vermare N, Dubois B, Caux C, Dalle S, Caramel J | 2022 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Cytonuclear, Multiplex IHC, Highplex FL |
| Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica | Hong Y, Shan S, Gu Y, Huang H, Zhang Q, Han Y, Dong Y, Liu Z, Huang M, Ren T | 2022 | Nature Communications | Other | Cytonuclear, Multiplex IHC |
| Sulforaphane exhibits antiviral activity against pandemic SARS-CoV-2 and seasonal HCoV-OC43 coronaviruses in vitro and in mice | Ordonez A, Bullen C, Villabona-Rueda A, Thompson E, Turner M, Merino V, Yan Y, Kim J, Davis S, Komm O, Powell J, D'Alessio F, Yolken R, Jain S, Jones-Brando L | 2022 | Communications Biology | Infectious Disease | Area Quantification, Multiplex IHC |
| Interspatial Distribution of Tumor and Immune Cells in Correlation with PD-L1 in Molecular Subtypes of Gastric Cancers | Dislich B, Mertz K, Gloor B, Langer R | 2022 | Cancers | Immuno-oncology | Multiplex IHC, Spatial Analysis, Figure Maker |
| Ad26.COV2.S prevents upregulation of SARS-CoV-2 induced pathways of inflammation and thrombosis in hamsters and rhesus macaques | Aid M, Vidal S, Piedra-Mora C, Ducat S, Chan C, Bondoc S, Colarusso A, Starke C, Nekorchuk M, Busman-Sahay K, Estes J, Martinot A, Barouch D | 2022 | PLOS Pathogens | Infectious Disease | Area Quantification, Multiplex IHC |
| Hyperpolarized 13C-Pyruvate Metabolism as a Surrogate for Tumor Grade and Poor Outcome in Renal Cell Carcinoma—A Proof of Principle Study | Ursprung S, Woitek R, McLean M, Priest A, Crispin-Ortuzar M, Brodie C, Gill A, Gehrung M, Beer L, Riddick A, Field-Rayner J, Grist J, Deen S, Riemer F, Kaggie J, Zaccagna F, Duarte J, Locke M, Frary A, Aho T, Armitage J, Casey R, Mendichovszky I, Welsh S, Barrett T, Graves M, Eisen T, Mitchell T, Warren A, Brindle K, Sala E, Stewart G, Gallagher F | 2022 | Cancers | Oncology | Area Quantification, Cytonuclear, Multiplex IHC |
| Therapeutic impact of BET inhibitor BI 894999 treatment: backtranslation from the clinic | Tontsch-Grunt U, Traexler P, Baum A, Musa H, Marzin K, Wang S, Trapani F, Engelhardt H, Solca F | 2022 | British Journal of Cancer | Oncology | Classifier, Cytonuclear, Multiplex IHC |
| Organelle resolved proteomics reveals new chordoma cell surface markers required for proliferation and association with outcome | Khan S, Zuccato J, Ignatchenko V, Singh O, Govindarajan M, Waas M, Gao A, Zadeh G, Kislinger T | 2022 | Research Square | Oncology | Cytonuclear, Multiplex IHC |
| Immune cell subsets in interface cutaneous immune-related adverse events (cirAEs) associated with anti-PD-1 therapy resemble accute graft vs host disease more than licen planus | Cruz G, Kaunitz G, Stein J, Sander I, Hollmann T, Cottrell T, Taube J, Sunshine J | 2022 | Journal of Cutaneous Pathology | Immunology | Cytonuclear, Multiplex IHC |
| Immune and epithelial determinants of age-related risk and alveolar injury in fatal COVID-19 | Chait M, Yilmaz M, Shakil S, Ku A, Dogra P, Connors T, Szabo P, Gray J, Wells S, Kubota M, Matsumoto R, Poon M, Snyder M, Baldwin M, Sims P, Saqi A, Farber D, Weisberg S | 2022 | JCI Insight | Infectious Disease | Multiplex IHC |