Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

ILLUMINATED. With the PATHOLOGY HALO® IMAGE ANALYSIS PLATFORM , the gold standard analysis solution for digital pathology. PATHOLOGY ILLUMINATED.

Slide With the HALO® IMAGE ANALYSIS PLATFORM

HALO®

IMAGE ANALYSIS PLATFORM

HALO is the gold standard image analysis platform for quantitative tissue analysis in digital pathology.

With unmatched ease-of-use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, pharmaceutical, healthcare and research organizations worldwide are using HALO for high-throughput, quantitative tissue analysis in oncology, neuroscience, metabolism, toxicology and more. 

HALO®

IMAGE ANALYSIS PLATFORM

HALO is the gold standard image analysis platform for quantitative tissue analysis in digital pathology.

With unmatched ease-of-use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, pharmaceutical, healthcare and research organizations worldwide are using HALO for high-throughput, quantitative tissue analysis in oncology, neuroscience, metabolism, toxicology and more.  

EASY-TO-USE MODULES & ADD-ONS

HALO modules simplify the analysis workflow so there is no need to ‘build’ algorithms from scratch. Modules make HALO easy-to-use and understand which means you can spend less time learning software and more time analyzing data.

HALO add-ons can be used in combination with any of the other analysis modules to improve the analysis workflow. These include tools to help automate tissue segmentation, such as tissue classifier, serial section analysis, and TMA, as well as tools to improve image processing speeds for high-throughput labs, such as analysis cluster.

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EXTENSIVE ANALYTICS

HALO reports morphological and multiplexed expression data on a cell-by-cell basis across entire tissue sections and maintains an interactive link between cell data and cell image. Click on any cell in the image and immediately see analysis outputs for that specific cell. Sorting and filtering capabilities allow the user to mine millions of cells while visually assessing corresponding cell populations. For example, sort cells according to biomarker intensity and immediately locate cells with highest intensity in the image. Just imagine the endless possibilities.

EXTENSIVE ANALYTICS

HALO reports morphological and multiplexed expression data on a cell-by-cell basis across entire tissue sections and maintains an interactive link between cell data and cell image. Click on any cell in the image and immediately see analysis outputs for that specific cell. Sorting and filtering capabilities allow the user to mine millions of cells while visually assessing corresponding cell populations. For example, sort cells according to biomarker intensity and immediately locate cells with highest intensity in the image. Just imagine the endless possibilities.

SCALABLE PLATFORM

HALO’s modular design and flexible licensing allow it to be adapted to any requirement and budget. Start with all the modules or just a few and add more modules as your needs change. Use HALO on a single workstation or ramp up to a server-based license for your entire group. Purchase a permanent license or renew annually.

Discover
HALO Modules

Click on the links below to explore our most popular HALO modules & add-ons. Use the category filters or click the 'Load More' button at the bottom of the list to view additional options.

Highplex FL
Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.
Highplex FL
Multiplex IHC
Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.
Multiplex IHC
FREE TRIAL
Click here to fill out a demo request form. Upload up to three images if you would like to test analysis on your own slides.
Upload Images
Tissue Classifier Add-on
Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjuction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.
Tissue Classifier Add-on
Spatial Analysis
Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.
Spatial Analysis
ISH
Quantify spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH (RNAScope®)
ISH-IHC
Quantify IHC positivity and spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH-IHC (RNAScope®)
FISH-IF
Quantify an unlimited number of fluorescent RNA/DNA probes and IF protein biomarkers on a per cell basis. Includes support for fluorescent RNAscope® assays.
FISH-IF (RNAScope®)
Multiplex FISH
Quantify number, intensity and co-expression of an unlimited number of fluorescently labeled RNA or DNA probes on a per cell basis. Includes support for fluorescent RNAscope ® assays.
Multiplex FISH (RNAScope®)
Tissue Microarray (TMA) Add-on
Module to assist in the segmentation of TMA spots for further analysis.
Tissue Microarray (TMA) Add-on
Area Quantification
Deconvolve up to five colors in brightfield and measure positive area and average optical density for each stain and stain colocalization (where applicable).
Area Quantification
Cytonuclear IHC
Quantify expression of up to four brightfield cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear IHC
Area Quantification FL
Measure the positive area, average intensity and dye colocalization (where applicable) of an unlimited number of fluorescent dyes.
Area Quantification FL
Cytonuclear FL
Quantify expression of an unlimited number of fluorescently labeled cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear FL
Membrane IHC
Quantify intensity of brightfield membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane IHC
Membrane FL
Quantify intensity of fluorescently-labeled membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane FL
Object Colocalization
Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization
Vacuole
Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.
Vacuole
Object Colocalization FL
Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization FL
Muscle Fiber
Quantifies area, diameter, and perimeter of muscle fibers stained with laminin or other fiber membrane stain.
Muscle Fiber
Muscle Fiber FL
Quantifies area, diameter, and perimeter of fluorescently-labelled muscle fibers stained with membrane markers, such as laminin.
Muscle Fiber FL
Branch Structure
Quantify branched objects such as capillaries in brightfield and fluorescence.
Branch Structure
Islet IHC
Count pancreatic islets, quantify number of alpha and beta cells in each islet, and determine area of islets stained with insulin and glucagon or other islet-specific stains
Islet IHC
Islet FL
Count pancreatic islets fluorescently-labeled with up to three dyes, quantify number of dye positive cells per islet and area of dye positivity per islet.
Islet FL
Microglial Activation
Quantify microglial activation based on length and thickness of microglial processes.
Microglial Activation
Amplification & Deletion FISH
Quantify up to two fluorescently-labeled DNA probes to measure amplification or deletion.
Amplification & Deletion FISH
Break Apart Fusion FISH
Meaure gene rearrangements detected using break-apart fusion probes.
Break Apart Fusion FISH
Serial Registration Analysis Add-on
Analyze serially stained tissue sections stained with different markers or a single tissue section which has been stained, stripped and re-stained for multiple markers.
Serial Section Analysis Add-on
Analysis Cluster Add-on
Designed for organization with large volume of slides or users, analysis clusters speed up analysis by facilitating simultaneous processing of multiple images in parallel.
Analysis Cluster Add-on
Axon
Quantify axons in cross sections. Calculate axon area, myelin area, myelin thickness, inner and outer axon diameter, and G-ratio.
Axon

Discover HALO Modules

Click on the links below to explore our most popular HALO modules & add-ons. Use the category filters or click the 'Load More' button at the bottom of the list to view additional options.

Highplex FL
Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.
Highplex FL
Multiplex IHC
Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.
Multiplex IHC
FREE TRIAL
Click here to fill out a demo request form. Upload up to three images if you would like to test analysis on your own slides.
Upload Images
Tissue Classifier Add-on
Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjuction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.
Tissue Classifier Add-on
Spatial Analysis
Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.
Spatial Analysis
ISH
Quantify spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH (RNAScope®)
ISH-IHC
Quantify IHC positivity and spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH-IHC (RNAScope®)
FISH-IF
Quantify an unlimited number of fluorescent RNA/DNA probes and IF protein biomarkers on a per cell basis. Includes support for fluorescent RNAscope® assays.
FISH-IF (RNAScope®)
Multiplex FISH
Quantify number, intensity and co-expression of an unlimited number of fluorescently labeled RNA or DNA probes on a per cell basis. Includes support for fluorescent RNAscope ® assays.
Multiplex FISH (RNAScope®)
Tissue Microarray (TMA) Add-on
Module to assist in the segmentation of TMA spots for further analysis.
Tissue Microarray (TMA) Add-on
Area Quantification
Deconvolve up to five colors in brightfield and measure positive area and average optical density for each stain and stain colocalization (where applicable).
Area Quantification
Cytonuclear IHC
Quantify expression of up to four brightfield cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear IHC
Area Quantification FL
Measure the positive area, average intensity and dye colocalization (where applicable) of an unlimited number of fluorescent dyes.
Area Quantification FL
Cytonuclear FL
Quantify expression of an unlimited number of fluorescently labeled cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear FL
Membrane IHC
Quantify intensity of brightfield membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane IHC
Membrane FL
Quantify intensity of fluorescently-labeled membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane FL
Object Colocalization
Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization
Vacuole
Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.
Vacuole
Object Colocalization FL
Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization FL
Muscle Fiber
Quantifies area, diameter, and perimeter of muscle fibers stained with laminin or other fiber membrane stain.
Muscle Fiber
Muscle Fiber FL
Quantifies area, diameter, and perimeter of fluorescently-labelled muscle fibers stained with membrane markers, such as laminin.
Muscle Fiber FL
Branch Structure
Quantify branched objects such as capillaries in brightfield and fluorescence.
Branch Structure
Islet IHC
Count pancreatic islets, quantify number of alpha and beta cells in each islet, and determine area of islets stained with insulin and glucagon or other islet-specific stains
Islet IHC
Islet FL
Count pancreatic islets fluorescently-labeled with up to three dyes, quantify number of dye positive cells per islet and area of dye positivity per islet.
Islet FL
Microglial Activation
Quantify microglial activation based on length and thickness of microglial processes.
Microglial Activation
Amplification & Deletion FISH
Quantify up to two fluorescently-labeled DNA probes to measure amplification or deletion.
Amplification & Deletion FISH
Break Apart Fusion FISH
Meaure gene rearrangements detected using break-apart fusion probes.
Break Apart Fusion FISH
Serial Registration Analysis Add-on
Analyze serially stained tissue sections stained with different markers or a single tissue section which has been stained, stripped and re-stained for multiple markers.
Serial Section Analysis Add-on
Analysis Cluster Add-on
Designed for organization with large volume of slides or users, analysis clusters speed up analysis by facilitating simultaneous processing of multiple images in parallel.
Analysis Cluster Add-on
Axon
Quantify axons in cross sections. Calculate axon area, myelin area, myelin thickness, inner and outer axon diameter, and G-ratio.
Axon

 Podcasts

Check out the latest National Society for Histotechnology HistoHelp podcast with Dave Krull and Adam Smith!
Click the tab on the below to listen!

HistoHelp Podcasts: Listen Now

In the first episode of HistoHelp Season 2, Artificial Intelligence: Not Scary Afterall David Krull gives a great intro to AI for Digital Pathology. And even a shout out on the ease of use of HALO AI! Click the play button below to listen and learn how AI is being used to improve patient diagnosis.

 

In Part 1 of the HistoHelp AI in the Lab podcast, David Krull, the NSH podcast expert and AI advocate, talks to Adam Smith, Director of Life Science Applications at Indica Labs, about approaches to implement and validate AI in the lab. Click the play button below to listen and learn a little bit about how to train AI algorithms!

 

In the first episode of HistoHelp Season 2, Artificial Intelligence: Not Scary Afterall David Krull gives a great intro to AI for Digital Pathology. And even a shout out on the ease of use of HALO AI! Click the play button below to listen and learn how AI is being used to improve patient diagnosis.

 

Platform Compatibility

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

File Formats

Non-proprietary (JPG, TIF, OME.TIFF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Ventana (BIF)
Philips (iSyntax)
KFBIO (KFB)

HALO Application Notes

Follow the links below to explore a few of the most popular HALO applications.

Fluorescence

Analyze Multiplexed Fluorescent Biomarkers in Whole Slide PerkinElmer Vectra® Polaris images fused in HALO

We demonstrate how HALO can create a multispectral 7-plex whole slide image from individual field-of-view image tiles captured from the PerkinElmer Vectra 3 or Polaris platform and analyze these images using our highplex FL module and spatial analysis modules.

Read More

Brightfield

Measure immune cell density and spatial distance to tumor cells within the tumor and invasive margin

Using our multiplex IHC module, we quantify immune cells and tumor cell markers across whole slide images.  The tissue classifier add-on is employed to segment tumor and stroma and the spatial analysis module is used for proximity and invasive margin analysis.  

Read More

Brightfield and Fluorescence

Quantify Single and Multiplexed RNAscope® probes across whole slide tissues

Using our ISH module for brightfield and our FISH module for fluorescence, we demonstrate how to quantify ISH signal from RNAScope® assays on a per cell or per unit area basis and output an overall expression score based on ACD Bio’s recommended scoring guidelines.  

Read More

Read what our customers are publishing

Our customers are making vital discoveries in oncology, neuroscience, and diabetes research.  Check out some of the research our customers are publishing using the HALO Image Analysis Platform and Indica analysis modules.

Click the button above to check out the latest high-impact publications where HALO image analysis was used. 

The digital quantification of PD1T cells has strong potential to guide the treatment of lung cancer patients with PD-1 inhibitors... The HALO platform strongly facilitated the research and development of this novel predictive indicator for clinical translation

- Dr. Viktor Koelzer, Pathologist and SNSF research fellow Institute of Cancer and Genomic Science, University of Birmingham

As a CRO specifically offering detailed high precision histopathology IA services, we chose the HALO platform as it enables us to develop tailored image analysis algorithms and generate highly detailed and robust data in a timely manner to address key questions our Clients face when interpreting drug efficacy, safety or biomarker related responses from their histology studies.

- John Waller, Phd, Chief Operations Officer, OracleBio Ltd

In my opinion the “ease-of-use” with the Halo system is second to none particularly when I think of some of the issues we faced in the past. Even when compared with other current systems Halo is being recognised by major Pharma companies, that run other more expensive mainframe type analysis systems, for the “ease-of-use” aspect...

- David Fairley, Managing Director, Histologix

Top Notch Training & Support

We’ve worked hard to ensure our HALO platform interfaces are the most intuitive in the industry, but we realize that you may still need extra support.  We’ve got you covered! 

Unlimited IT support, scientific support & training are included with every license at no additional cost.  With support staff in the US, UK and Japan, you are supported in any time zone.  Need help tuning an algorithm or training for new staff – we are just a call away.   

The vast majority of our new customers heard about Indica Labs from an existing customer.  The two primary reasons for referral – our user-friendly technology and excellent support.  We know that you have a choice in image analysis providers and we strive to make sure every customers is 100% satisfied.

Top Notch Training & Support

We’ve worked hard to ensure our HALO platform interfaces are the most intuitive in the industry, but we realize that you may still need extra support.  We’ve got you covered! 

Unlimited IT support, scientific support & training are included with every license at no additional cost.  With support staff in the US, UK and Japan, you are supported in any time zone.  Need help tuning an algorithm or training for new staff – we are just a call away.   

The vast majority of our new customers heard about Indica Labs from an existing customer.  The two primary reasons for referral – our user-friendly technology and excellent support.  We know that you have a choice in image analysis providers and we strive to make sure every customers is 100% satisfied.

Want to see more?

Fill out the form below to request a recorded or live demo of HALO or any of our other products or services.
You can also drop us an email at info@indicalab.com

We highly recommend using Google Chrome when filling out the info above. Please email marketing@indicalab.com for support.