The Indica Labs’ Membrane FL Quantification module measures fluorescently-labeled membranous markers on a cell-by-cell basis and scores cells according to membrane marker intensity. The module is color configurable and can be used to quantify any fluorescent dye that can be imaged.
In the example above, the software measures Her2 expression in breast tissue. Nuclei and membranes are identified and matched for each cell. Cells are then classified as negative, weak, moderate, or strong positive. Positive cell membranes are color coded yellow, orange, and red, dependent on their degree of positivity. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score). It can also report individual expression profiles for each cell.