Membrane FL

MEMBRANE FL

 

Product Overview

The Indica Labs’ Membrane FL Quantification module measures fluorescently-labeled membranous markers on a cell-by-cell basis and scores cells according to membrane marker intensity. The module is color configurable and can be used to quantify any fluorescent dye that can be imaged.

In the example above, the software measures Her2 expression in breast tissue. Nuclei and membranes are identified and matched for each cell. Cells are then classified as negative, weak, moderate, or strong positive. Positive cell membranes are color coded yellow, orange, and red, dependent on their degree of positivity. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score). It can also report individual expression profiles for each cell.

The Membrane FL module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

MEMBRANE FL

The Indica Labs’ Membrane FL Quantification module measures fluorescently-labeled membranous markers on a cell-by-cell basis and scores cells according to membrane marker intensity. The module is color configurable and can be used to quantify any fluorescent dye that can be imaged.

In the example above, the software measures Her2 expression in breast tissue. Nuclei and membranes are identified and matched for each cell. Cells are then classified as negative, weak, moderate, or strong positive. Positive cell membranes are color coded yellow, orange, and red, dependent on their degree of positivity. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score). It can also report individual expression profiles for each cell.

Here are a few publications that cite use of our Membrane IHC or FL modules.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Implementation of PD-L1 22C3 IHC pharmDxTM in Cell Block Preparations of Lung Cancer: Concordance with Surgical Resections and Technical Validation of CytoLyt® PrefixationLou SK, Ko HM, Kinoshita T, MacDonald S, Weiss J, Czarnecka-Kujawa K, Boerner SL, Yasufuku K, Tsao M-S, Schwock J2020Acta CytologicaOncologyClassifier, Membrane
Prevalence of CD8+ cytotoxic lymphocytes in human neoplasmsBlessin NC, Spriestersbach P, Li W, Mandelkow T, Dum D, Simon R, Hube-Magg C, Lutz F, Viehweger F, Lennartz M, Fraune C, Nickelsen V, Fehrle W, Göbel C, Weidemann S, Clauditz T, Lebok P, Möller K, Steurer S, Izbicki JR, Sauter G, Minner S, Jacobsen F, Luebke AM, Büscheck F, Höflmayer D, Wilczak W, Burandt E, Hinsch A2020Cellular OncologyOncology, Immuno-oncologyMembrane, TMA
A feasibility study of combined epigenetic and vaccine therapy in advanced colorectal cancer with pharmacodynamic endpointBever KM, Thomas DL, Zhang J, Rivera EAD, Rosner GL, Zhu Q, Nauroth JM, Christmas B, Thompson ED, Anders RA, Judkins C, Liu M, Jaffee EM, Ahuja N, Zheng L, Azad NS2021Clinical EpigeneticsOncologyImmune, Membrane
Treatment with the WNT5A-mimicking peptide Foxy-5 effictively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model Canesin G, Evans-Axelsson S, Hellsten R, Krzyzanowska A, Prasad CP, Bjartell A, Andersson T2017PLOS OneOncologyMembrane
STAT3 inhibition with galiellalactone efectively targets the prostate cancer stem‑like cell populationCanesin G, Maggio V, Palominos M, Stiehm A, Contreras HR, Castellon EA, Morote J, Paciucci R, Maitland NJ, Bjartell A, Hellsten R2020Scientific ReportsOncologyMembrane
Expression of PD-L1 and presence of CD8-positive T cells in pre-treatment specimens of locally advanced cervical cancer Enwere EK, Kornaga EN, Dean M, Koulis TA, Phan T, Kalantarian M, Köbel M, Ghatage P, Magliocco AM, Lees-Miller DP, Doll CM2017Modern PathologyOncology, Immuno-oncologyMembrane
What is the added value of digital image analysis of HER2 immunohistochemistry in breast cancer in clinical practice? A study with multiple platforms Koopman T, Buikema HJ, Hollema H, de Bock GH, van der Vegt B2018HistopathologyOncologyMembrane
GPNMB expression identifies TSC1/2/mTOR-associated and MiT family translocation-driven renal neoplasmsSalles D, Asrani K, Woo J, Vidotto T, Liu H, Vidal I, Matoso A, Netto G, Argani P, Lotan T2022The Journal of PathologyOncologyMembrane
e The Association of Cholesterol Uptake and Synthesis with Histology and Genotype in Cortisol-Producing Adenoma (CPA)Motomura N, Yamazaki Y, Koga D, Harashima S, Gao X, Tezuka Y, Omata K, Ono Y, Morimoto R, Satoh F, Nakamura Y, Kwon G, Choi M, Ito A, Sasano H2022International Journal of Molecular SciencesArea Quantification, Cytonuclear, Membrane
Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancerSushentsev N, McLean M, Warren A, Benjamin A, Brodie C, Frary A, Gill A, Jones J, Kaggie J, Lamb B, Locke M, Miller J, Mills I, Priest A, Robb F, Shah N, Schulte R, Graves M, Gnanapragasam V, Brindle K, Barrett T, Gallacher F2022Nature CommunicationsOncologyISH/FISH, Membrane, Multiplex IHC
Haploinsufficiency of the lysosomal sialidase NEU1 results in a model of pleomorphic rhabdomyosarcoma in miceMachado E, van de Vlekkert D, Sheppard H, Perry S, Downing S, Laxton J, Ashmun R, Finkelstein D, Neale G, Hu H, Harwood F, Koo S, Grosveld G, d'Azzo A2022Communications BiologyOncologyMembrane, Spatial Analysis
Phenotype-genotype correlation in aldosterone-producing adenomas characterized by intracellular cholesterol metabolismHarashima S, Yamazaki Y, Motomura N, Ono Y, Omata K, Tezuka Y, Morimoto R, Nakamura Y, Satoh F, Suzuki H, Kwon G, Choi M, Sasano H2022The Journal of Steroid Biochemistry and Molecular BiologyOncology, MetabolismArea Quantification, Membrane

Click here to initiate your free proof-of-concept HALO® image analysis.