Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

Membrane IHC

Membrane IHC

 

Product Overview

The Membrane IHC module measures membranous immunohistochemical markers on a cell-by-cell basis. This module is color configurable, so it can be used with non-traditional chromogens as well as with DAB. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score) and can be configured to report individual expression profiles for each cell.

In the example above, the software measures Her2 expression in breast tissue. Nuclei and membranes are identified and matched for each cell. Cells are then classified as negative, weak, moderate, or strong positive. Positive cell membranes are color coded yellow, orange, and red, dependent on their degree of positivity.

The Membrane IHC module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

Membrane IHC

The Membrane IHC module measures membranous immunohistochemical markers on a cell-by-cell basis. This module is color configurable, so it can be used with non-traditional chromogens as well as with DAB. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score) and can be configured to report individual expression profiles for each cell.

In the example above, the software measures Her2 expression in breast tissue. Nuclei and membranes are identified and matched for each cell. Cells are then classified as negative, weak, moderate, or strong positive. Positive cell membranes are color coded yellow, orange, and red, dependent on their degree of positivity.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Here are a few publications that cite use of our Membrane IHC or FL modules.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Implementation of PD-L1 22C3 IHC pharmDxTM in Cell Block Preparations of Lung Cancer: Concordance with Surgical Resections and Technical Validation of CytoLyt® PrefixationLou SK, Ko HM, Kinoshita T, MacDonald S, Weiss J, Czarnecka-Kujawa K, Boerner SL, Yasufuku K, Tsao M-S, Schwock J2020Acta CytologicaOncologyClassifier, Membrane
Prevalence of CD8+ cytotoxic lymphocytes in human neoplasmsBlessin NC, Spriestersbach P, Li W, Mandelkow T, Dum D, Simon R, Hube-Magg C, Lutz F, Viehweger F, Lennartz M, Fraune C, Nickelsen V, Fehrle W, Göbel C, Weidemann S, Clauditz T, Lebok P, Möller K, Steurer S, Izbicki JR, Sauter G, Minner S, Jacobsen F, Luebke AM, Büscheck F, Höflmayer D, Wilczak W, Burandt E, Hinsch A2020Cellular OncologyOncology, Immuno-oncologyMembrane, TMA
A feasibility study of combined epigenetic and vaccine therapy in advanced colorectal cancer with pharmacodynamic endpointBever KM, Thomas DL, Zhang J, Rivera EAD, Rosner GL, Zhu Q, Nauroth JM, Christmas B, Thompson ED, Anders RA, Judkins C, Liu M, Jaffee EM, Ahuja N, Zheng L, Azad NS2021Clinical EpigeneticsOncologyImmune, Membrane
Treatment with the WNT5A-mimicking peptide Foxy-5 effictively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model Canesin G, Evans-Axelsson S, Hellsten R, Krzyzanowska A, Prasad CP, Bjartell A, Andersson T2017PLOS OneOncologyMembrane
STAT3 inhibition with galiellalactone efectively targets the prostate cancer stem‑like cell populationCanesin G, Maggio V, Palominos M, Stiehm A, Contreras HR, Castellon EA, Morote J, Paciucci R, Maitland NJ, Bjartell A, Hellsten R2020Scientific ReportsOncologyMembrane
Expression of PD-L1 and presence of CD8-positive T cells in pre-treatment specimens of locally advanced cervical cancer Enwere EK, Kornaga EN, Dean M, Koulis TA, Phan T, Kalantarian M, Köbel M, Ghatage P, Magliocco AM, Lees-Miller DP, Doll CM2017Modern PathologyOncology, Immuno-oncologyMembrane
What is the added value of digital image analysis of HER2 immunohistochemistry in breast cancer in clinical practice? A study with multiple platforms Koopman T, Buikema HJ, Hollema H, de Bock GH, van der Vegt B2018HistopathologyOncologyMembrane

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.