The Vacuole Quantification module is a multi-purpose module that can be used for quantifying the number and size of white spaces in tissue. Typical applications are lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung.
In the example above, the module was used to analyze an H&E stained fatty mouse liver. The nuclei are marked blue and the lipid vacuoles are marked red. The module reports total vacuole area, average vacuole area, diameter, perimeter, and total vacuole count. The module also generates a histogram representing the frequency of lipid vacuoles within a specified diameter range.