Indica
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Vacuole Quantification

VACUOLE QUANTIFICATION

 

Product Overview

The Vacuole Quantification module is a multi-purpose module that can be used for quantifying the number and size of white spaces in tissue. Typical applications are lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung.

In the example above, the module was used to analyze an H&E stained fatty mouse liver. The nuclei are marked blue and the lipid vacuoles are marked red. The module reports total vacuole area, average vacuole area, diameter, perimeter, and total vacuole count. The module also generates a histogram representing the frequency of lipid vacuoles within a specified diameter range.

The vacuole module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

VACUOLE QUANTIFICATION

The Vacuole Quantification module is a multi-purpose module that can be used for quantifying the number and size of white spaces in tissue. Typical applications are lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung.

In the example above, the module was used to analyze an H&E stained fatty mouse liver. The nuclei are marked blue and the lipid vacuoles are marked red. The module reports total vacuole area, average vacuole area, diameter, perimeter, and total vacuole count. The module also generates a histogram representing the frequency of lipid vacuoles within a specified diameter range.

Here are a few publications that cite the use of our Vacuole Quantification module. Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity Petkevicius K, Virtue S, Bidault G, Jenkins B, Cubuk C, Morgantini C, Aouadi M, Dopazo J, Serlie M, Koulman A, Vidal-Puig A2019bioRxivClassifier, Vacuole
A suboptimal maternal diet combined with accelerated postnatal growth results in an altered aging profile in the thymus of male rats Tarry-Adkins JL, Aiken CE, Ashmore TJ, Fernandez-Twinn DS, Chen J-H, Ozanne SE2018The FASEB JournalNeuroscience, MetabolismClassifier, Vacuole
Phenotypic characterization of Adig null mice suggests roles for adipogenin in the regulation of fat mass accrual and leptin secretionAlvarez-Guaita A, Patel S, Lim K, Haider A, Dong L, Conway OJ, Ma MKL, Chiarugi D, Saudek V, O'Rahilly S, Savage DB2021Cell ReportsMetabolismVacuole
Breast adipocyte size associates with ipsilateral invasive breast cancer risk after ductal carcinoma in situAlmekinders MMM, Schaapveld M, Thijssen B, Visser LL, Bismeijer T, Sanders J, Isnaldi E, Hofland I, Mertz M, Wessels LFA, Broeks A, Hooijberg E, Zwart W, Lips EH, Grand Challenge PRECISION Consortium, Desmedt C, Wesseling J2021npj Breast CancerOncologyVacuole
Allostatic hypermetabolic response in PGC1?/? heterozygote mouse despite mitochondrial defectsRodriguez-Cuenca S, Lelliot C, Campbell M, Peddinti G, Martinuez-Uña M, Ingvorsen C, Dias A, Relat J, Mora S, Hyötyläinen T, Zorzano A, Oreši? M, Bjursell M, Bohlooly-Y M, Lindén D, Vidal-Puid A2021The FASEB JournalMetabolismVacuole
AST-120 Treatment Alters the Gut Microbiota Composition and Suppresses Hepatic Triglyceride Levels in Obese MiceHiraga Y, Kubota T, Katoh, M, et al.2021Endocrine ResearchMetabolismVacuole
Allostatic hypermetabolic response in PGC1?/? heterozygote mouse despite mitochondrial defectsRodriguez-Cuenca S, Lelliot C, Campbell M, Peddinti G, Martinuez-Uña M, Ingvorsen C, Dias A, Relat J, Mora S, Hyötyläinen T, Zorzano A, Oreši? M, Bjursell M, Bohlooly-Y M, Lindén D, Vidal-Puid A2021The FASEB JournalMetabolismVacuole
AST-120 Treatment Alters the Gut Microbiota Composition and Suppresses Hepatic Triglyceride Levels in Obese MiceHiraga Y, Kubota T, Katoh, M, et al.2021Endocrine ResearchMetabolismVacuole
Evaluation of a PEGylated Fibroblast Growth Factor 21 Variant Using Novel Preclinical Magnetic Resonance Imaging and Magnetic Resonance Elastography in a Mouse Model of Nonalcoholic SteatohepatitisTang H, Li J, Zinker B, Boehm S, Mauer A, Rex-Rabe S, Glaser K, Fronheiser M, Bradstreet T, Nakao Y, Petrone T, Pena A, Villano M, Chow P, Malhi H, Charles E, Hayes W, Ehman R, Du S, Yin M2022Journal of Magnetic Resonance ImagingMetabolismVacuole
Obesity due to Steroid Receptor Coactivator-1 deficiency is associated with endocrine and metabolic abnormalitiesCacciottolo T, Henning E, Keogh J, Lassen P, Lawler K, Bounds R, Ahmed R, Perdikari A, Mendes de Oliverira E, Smith M, Godfrey E, Johnson E, Hodson L, Clement K, van der Klaauw A, Farooqi I2022The Journal of Clinical Endocrinology & MetabolismMetabolismVacuole

Click here to initiate your free proof-of-concept HALO® image analysis.