
Getting Started with Multiplex Fluorescence Image Analysis in HALO®
Date: 11 June 2025
Time: 8:00 – 9:00 EDT | 13:00 – 14:00 BST | 14:00 – 15:00 CEST
Location: Webinar
Learn the basics of multiplex fluorescence image analysis!
Summary
Join us for an introductory webinar on Multiplex fluorescence image analysis in HALO®, designed for beginners. The presentation will walk through key digital pathology workflows for fluorescence images, including optimization of cell identification using artificial intelligence and HALO’s new phenotype editor. You’ll also learn the fundamentals of AI pattern-based recognition for identifying cells or tissue structures as well as how multiparametric techniques can help uncover biological patterns.
The presentation and live demonstration will explore how pretrained models and custom classifiers developed using HALO AI can streamline workflows by automating tissue segmentation and cell phenotyping. Throughout the webinar, we will share tips and tricks to improve your analysis, avoid time-consuming pitfalls, and optimize your multiplex workflows.
Learning Objectives
- Understand the basics of fluorescence analysis and its applications in digital pathology
- Learn the fundamentals of using the HALO Highplex FL module for analyzing fluorescent markers in the nucleus, cytoplasm, and/or membrane
- Explore strategies for tissue segmentation and cell phenotyping using HALO’s AI-driven tools
- Understand the tools available in HALO for multiparametric data exploration
Presenter

Dr. Ghislaine Lioux
Senior Field Applications Scientist
Indica Labs
Ghislaine Lioux has a background in molecular biology and heart development. She was awarded a Marie Curie Fellowship and obtained her PhD from the Spanish National Institute of Cardiovascular Research in Madrid. Ghislaine brings with her 10 years of experience in the immunostaining and imaging world from various institutes in Spain, France, UK, and USA. As a Senior Field Applications Scientist at Indica Labs, Ghislaine works daily with scientists from pharmaceutical, healthcare, and research organizations worldwide and provides tailored sales and application support to customers.