Powering Discovery with AI
Quantitative and intuitive image analysis for pathology
Image Analysis Simplified
With unmatched ease-of-use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, research, pharma and healthcare organizations worldwide are using HALO® for high-throughput, quantitative tissue analysis.
Intuitive Platform for Analysts at All Levels
An image analyst can be up and running with basic analysis functions in just a couple of hours with the user-friendly HALO platform – even with no prior image analysis experience!
HALO modules simplify the analysis workflow so there is no need to ‘build’ algorithms from scratch. Our purpose-built modules make HALO easy-to-use and understand which means you can spend less time learning software and more time analyzing data.
Extensive Analytics, Reliable Results
HALO reports morphological and multiplexed expression data on a cell-by-cell basis across entire tissue sections while maintaining an interactive link between cell data and image. Sort and filter cell data to mine millions of cells while also visually assessing cell populations in the context of tissue architecture, leverage interactive markups to understand colocalization and explore combined phenotypes, and define custom analysis outputs.
Leverage Integrated AI Tools
Use pre-trained deep-learning networks for optimized nuclear and membrane segmentation directly in the HALO platform with cell analysis modules in brightfield or fluorescence, including the Multiplex IHC and Highplex FL modules.
Want to train your own AI? Learn more about HALO AI.
Rapid Analysis and Efficient Workflows on a Scalable Platform
Increase your productivity with multi-core processing and batch analysis or with workflow tools for efficient tissue microarray and serial stain analysis.
1. Choose Modules
Options include analysis and workflow modules.
2. Choose License
Single seat, multiple seat & enterprise license options available.
3. Choose Term
License HALO on an annual or permanent basis.
4. Choose Deployment
Manage your own local or cloud deployment, or work with Cloud Services.
The modular design and flexible licensing of HALO allow it to be adapted to any requirement. Start with a few modules and add more as your needs change. Use HALO on a single workstation, on a server-based license or deploy enterprise wide. Choose either a permanent license or renew annually and choose the deployment option that works best for your team and local IT.
Highly Interoperable Ecosystem
HALO has a variety of data export and import tools so that you can work with your data outside of the HALO platform and then bring it back in.
HALO also seamlessly integrates with our deep learning toolbox HALO AI, our study-based image management system, HALO Link, and our case-based image management system, HALO AP®.
Want to connect to another image management system? Custom integrations are available using a powerful and open GraphQL API. Reach out to info@indicalab.com to learn more.
Contribute to the Growing List of High Impact HALO Publications
HALO image analysis appears in over 1,000 peer-reviewed publications and every year the number of publications continues to grow!
Our customers publish across a wide range of disciplines and research areas, with oncology and immuno-oncology leading the publication count, shortly followed by neuroscience, metabolism, immunology, and infectious disease.
Curious to learn more? Check out the Publications tab in the HALO Knowledge Center.
HALO® Peer-Reviewed Publications
Top Notch Training and Support
We’ve worked hard to ensure HALO is the most intuitive image analysis platform in the industry, but we realize that you may still need extra support. We’ve got you covered!
Unlimited IT support, scientific support, training, and access to the Learning Portal are included with every license at no additional cost. With support staff in the US, UK, EU, China, and Japan, you are supported in any time zone.
The vast majority of our new customers heard about Indica Labs from an existing customer. The two primary reasons for referral – our user-friendly technology and excellent support. We know that you have a choice in image analysis providers, and we strive to make sure every customer is 100% satisfied.
Platform Compatibility
File Formats:
- Non-proprietary (JPG, TIF, OME.TIFF)
- Nikon (ND2)
- 3DHistech (MRXS)
- Akoya (QPTIFF, component TIFF)
- Olympus / Evident (VSI)
- Hamamatsu (NDPI, NDPIS)
- Aperio (SVS, AFI)
- Zeiss (CZI)
- Leica (SCN, LIF)
- Ventana (BIF)
- Philips (iSyntax, i2Syntax)
- KFBIO (KFB, KFBF)
- DICOM (DCM*)
*whole-slide images
HALO® is compatible with a broad spectrum of image and digital slide formats. Yours not on the list? Email us your requirements.
Powering Discovery with AI: Features of the HALO Image Analysis Platform
Investigate results with interactive markup images where you can toggle on and off each cell population of interest.
Discover HALO® Modules
Explore our most popular HALO modules & add-ons. You can filter by category and use the arrows at the bottom of the list to view additional options.
Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.
Learn MoreQuantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.
Learn MoreSeparate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.
Learn MorePlot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.
Learn MoreSimultaneously analyze up to three chromogenic and/or silver-labelled DNA or RNA ISH probes on a cell-by-cell basis, measuring spot numbers and area per cell and compartment, and calculated H-scores for each probe.
Learn MoreSimultaneously analyze a nuclear stain and up to four IHC biomarkers or ISH probes on a cell-by-cell basis across brightfield images.
Learn MoreSimultaneously analyze an unlimited number of fluorescent nucleic acid probes on a cell-by-cell basis, measuring spot numbers and area per cell and compartment, and calculated H-scores for each probe.
Learn MoreUse the HALO® FISH-IF module and reagents from Molecular Instruments or ACD, a Bio-techne brand, to simultaneously analyze an unlimited number of fluorescently-labeled DNA/RNA ISH probes and immunofluorescent protein biomarkers on a cell-by-cell basis.
Learn MoreThe TMA Add-on provides a productivity-enhancing workflow for tissue microarray analysis by enabling automated, high-throughput segmentation and batch analysis of whole slide TMA images.
Learn MoreWhat Our Customers Have to Say
Read independent reviews on our HALO image analysis platform and learn how these customers are using Indica Labs’ solutions to streamline their workflows.
HALO has become a fundamental tool in our research activity.
"Our Lab (Tumor Immunology Laboratory, University of Palermo) investigates immune responses in cancer. We adopted several tools for quantitative assessment of immunolabeling with good results. HALO offers high-quality cell segmentation and allows accurate analysis of both brighfield and fluorescence-based microphotographs or slide scans. The possibility to easily extract cell features integrating spatial information enables detailed spatial interaction analyses."
Claudio Tripodo
University of Palermo, Tumor Immunology Laboratory
Excellent results, easy to use and flexible. A must-have for my work!
"Easy-to-use software, interactive and well-designed. Halo software is a must-have for our research for several years. Frequent update give more and more possibility to analyze our images. And let's not forget the incredible work and reactivity of all the people in indica labs helping us when we have some trouble to optimize our algorithm. They saved us a lot of time with at a speed I have never seen elsewhere!"
Yoan VELUT
Cordeliers Research Center
HALO is a user-friendly & powerful tool for image analysis w/ great technical support
"HALO is extremely straight forward and easy to use. Great and useful features include: 1. The real-time tuning tool to allow a live view of the effects of various analysis parameters for easy optimization. 2. Annotation tools make it easy to select regions of interest. 3. The ability to perform batch analysis on many images at once. 4. Useful and handy multi-panel figure maker that makes it easy for publication."
Joey Orpilla
UCLA
Could not survive without this software
"HALO is user-friendly, and invaluable for quantitation of multi-omics imaging data (MIBI, CODEX/Akoya multiplex stains, RNA-ISH/RNAscope). Cell segmentation, quantitation of tissue microarray data, aligning serial images, exporting quantitative data and .fcs files, it is incredible. The support team is incredibly responsive and collaborative. HALO is a bit expensive, but it has been completely worth it for us, and it was a worthwhile investment. Highly recommend a detailed demo and purchasing this software if you are interested in quantitative data from tissue multi-omics."
Michael Kattah
UCSF
Excellent replacement for older immunohistochemistry analysis software
"The software is modular and can be run on a server. This helps to increase the analysis power and while reducing the hardware requirement to do the analysis from individual's side. It accepts multiple image formats for analysis which is a plus. The representatives were professional and helped in setting up more modules with ease. Since we have used other software, migrating to this one was easy and has few more analysis set up which was useful and increased its value among our researchers."
Muralidharan Jayaraman
University of Oklahoma Health Sciences Center
We use HALO & HALO-Link daily to coordinate studies for our analysts and pathologists.
"HALO is a whole-slide image analysis platform that is designed to work how pathologists classify & score images using a microscope. It uses a set of tools that are very familiar to anyone who has used any type of image viewing/editing program at home. It can be learned in a day to analyze brightfield & fluorescent whole slide images and TMAs. There are sophisticated tools to analyze tissues labeled by chromogenic and fluorescent IHC, RNAScope, FISH & ISH. HALO may be installed on stand-alone workstations or cloud services such as AWS. File sharing through HALO-Link lets user access studies through a simple web-interface sharing a common database. HALO AI allows any user to developing sophisticated AI based tissue classification without the need for external programs or expensive computing hardware."
Timothy Baradet
Bristol-Myers Squibb
For the Pathologist HALO is by far the best digital image analysis platform available.
"As a Pathologist specializing in digital image analysis, I have had the opportunity to use several different digital image analysis platforms. Indica Labs' HALO is by far the most accurate, versatile and user friendly digital image analysis platform that I have used. Literally within 10-15 minutes of "tuning" one of their "out of the box" detection algorithms, I can perform image analysis for most simple immunohistochemical markers. The workflow is simple, with sacanned images being imported directly into HALO with or without annotations for analysis. This even allows for remote use when working off site. The customer support is AMAZING and I routinely get same day or next day help from the technical support reps. HALO is a must have for any pathologist or scientist involved in quantifying immunohistochemical stains. In addition the HALO AI module is extremely easy to use. Within 20 minutes I was able to set up AI detection of tumor vs. normal tissue, as well as tumor epithelium vs. stroma. HALO provides everything one needs for quantification of IHC for clinical and pre-clinical studies (of course with supervision of a pathologist). Best of all: it is fun, like a video game for Pathologists!"
Philip Martin
AstraZeneca
Deploy HALO® in a Fully Managed Cloud-Hosted Environment
Indica Labs offers optimized deployments of HALO software hosted in Amazon Web Services (AWS). We manage all aspects of implementation and ongoing maintenance to provide a highly performant, scalable, and secure HALO environment in the cloud.
You maintain full ownership over your AWS account while our AWS Certified Solutions Architects manage your infrastructure and HALO software environments.
Learn more about benefits of cloud-hosted environments in our recent blog post. Or reach out today to our Cloud Services team by using the form below, to learn whether a managed cloud deployment is right for you.
Knowledge Center
Learn more about HALO® image analysis by exploring the tabs below.
Spatial Analysis Application Note
In this application note, we describe two HALO analysis tools that are used to quantify the distribution of immune cells relative to tumor cells in the tumor microenvironment, Proximity Analysis and Tumor Infiltration Analysis.
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Fusion of Spectrally Unmixed Image Tiles into Whole Slide TIFs and HALO Image Analysis
In this application note, we describe how HALO can convert spectrally unmixed image tiles into whole slide images for analysis of the tumor microenvironment.
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Quantify Single and Multiplexed RNAscope® Probes Across Whole Slide Tissues
Using our ISH module for brightfield and our FISH module for fluorescence, we demonstrate how to quantify ISH signal from RNAscope® assays on a per cell or per unit area basis and output an overall expression score based on ACD Bio’s recommended scoring guidelines.
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HiPlex RNAscope™ Application Note
Using our FISH module we demonstrate how to perform 12-plex RNAscope image analysis using ACD’s HiPlexv2 assay.
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HALO Brochure
Download our HALO brochure to learn about the features and benefits of the gold standard image analysis platform for quantitative tissue analysis in digital pathology.
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HALO – Image Analysis Platform (简体中文 Simplified Chinese)
Download our HALO brochure in simplified Chinese 简体中文 to learn about the features and benefits of the gold standard image analysis platform for quantitative tissue analysis in digital pathology.
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HALO – Image Analysis Platform (日本 Japanese)
Download our HALO brochure in 日本 Japanese to learn about the features and benefits of the gold standard image analysis platform for quantitative tissue analysis in digital pathology.
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HALO, HALO AI, & HALO Link Cloud Services Brochure
Discover how our Cloud Services team can provide a secure, scalable, and flexible cloud deployment of HALO, HALO AI, and HALO Link.
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NCI Case Study
Download this case study to learn about considerations the National Cancer Institute made when planning to migrate its HALO, HALO AI, and HALO Link deployments to the cloud, and benefits the NCI experienced after migration, including 50% faster HALO image analysis.
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Cloud Services and AI Diagnostics Case Study
Learn how the optimized, scalable infrastructure provided by Indica Labs Cloud Services enables the rapid development and robust collaboration of our AI Diagnostics group, powering the next generation of automated AI-based decision support tools.
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Clustering Workflow eBook
Check out our collaborative eBook with Lunaphore to learn about an optimized workflow for extracting information from hyperplex datasets and illuminating changes in cellular neighborhoods using HALO, HALO AI, and the Lunaphore COMET platform.
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Lunaphore COMET Hyperplex IF and HALO® Image Analysis eBook
Our collaborative eBook with Lunaphore shows how HALO and HALO AI, alongside the Lunaphore COMET instrument, enable a high-throughput workflow for phenotypic and spatial analysis of cells in the tumor microenvironment.
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Sequential Same Slide mIF and H&E eBook
Reveal more data on a single slide with this workflow for sequential same slide multiplex IF and H&E staining and imaging using HALO and HALO AI.
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mIF Co-Registration and Cellular Analysis eBook
Learn how HALO offers a streamlined workflow for co-registration and cellular analysis of immunofluorescence images from cyclically stained and imaged slides.
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Qualitative and Quantitative Evaluation of the TME eBook
Check out this ebook to see how AI-based nuclear segmentation and tissue classification can streamline cell phenotyping across tissue compartments and samples.
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Lunaphore COMET Hyperplex IF and HALO® Image Analysis of the TME
Download our collaborative poster with Lunaphore to see how HALO and HALO AI, alongside the Lunaphore COMET instrument, enable a high throughput workflow for phenotypic and spatial analysis of cells in the tumor microenvironment.
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Clustering Workflow for Advanced Cell Phenotyping of Hyperplex IF Images
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Spatial Characterization of the TME with Cell DIVE Multiplexed Imaging and HALO Image Analysis
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Quantitative Image Analysis of a Combined FISH-IF Assay
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Characterizing the TME Using IMC and HALO Image Analysis
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Automated Tumor Budding Quantification in Colorectal Carcinoma H&E Images
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Spatial Multiplex Profiling of Immune Markers with the RNAscope Hiplex v2 Assay
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Evaluation of the TME by High Resolution 17-plex IF and HALO Image Analysis
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Our customers are making vital discoveries in oncology, neuroscience, and diabetes research. Check out some of the research our customers are publishing using the HALO Image Analysis Platform and Indica analysis modules.
Fetal growth restriction (FGR) is a significant concern in obstetrics, affecting 5–10% of pregnancies globally. FGR is a major contributor to perinatal morbidity and mortality and poses a significant maternal risk due to potential co-occurrence with pre-eclampsia. Despite their impact,...
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Learn MoreHALO® High Impact Publications
Learn how HALO image analysis is leveraged in fields from immuno-oncology to neuroscience and infectious disease in this selection of high-impact publications.