IMAGE ANALYSIS PLATFORM
HALO is the gold standard image analysis platform for quantitative tissue analysis in digital pathology.
With unmatched ease-of-use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, pharmaceutical, healthcare and research organizations worldwide are using HALO for high-throughput, quantitative tissue analysis in oncology, neuroscience, metabolism, toxicology and more.
HALO®
IMAGE ANALYSIS PLATFORM
HALO is the gold standard image analysis platform for quantitative tissue analysis in digital pathology.
With unmatched ease-of-use and scalability, powerful analytic capabilities, and the fastest processing speeds available for digital pathology, pharmaceutical, healthcare and research organizations worldwide are using HALO for high-throughput, quantitative tissue analysis in oncology, neuroscience, metabolism, toxicology and more.
RELATED RESOURCES
Brochure
Brochure (Simplified Chinese)
Brochure (Japanese)
White Paper
Sequential same slide mIF and H&E eBook
9-plex analysis eBook
Qualitative and quantitative evaluation of TME eBook
Transitioning to the Cloud: Establishing a Collaborative Digital Pathology Platform at the National Cancer Institute
RNAscope Publications
Collaborative Poster with Lunaphore
CONTACT US
For demo, trial, or quotation:
info@indicalab.com
+1 (505) 492-0979
EASY-TO-USE MODULES & ADD-ONS
HALO modules simplify the analysis workflow so there is no need to ‘build’ algorithms from scratch. Modules make HALO easy-to-use and understand which means you can spend less time learning software and more time analyzing data.
HALO add-ons can be used in combination with any of the other analysis modules to improve the analysis workflow. These include tools to help automate tissue segmentation, such as tissue classifier, serial section analysis, and TMA, as well as tools to improve image processing speeds for high-throughput labs, such as analysis cluster.
EXTENSIVE ANALYTICS
HALO reports morphological and multiplexed expression data on a cell-by-cell basis across entire tissue sections and maintains an interactive link between cell data and cell image. Click on any cell in the image and immediately see analysis outputs for that specific cell. Sorting and filtering capabilities allow the user to mine millions of cells while visually assessing corresponding cell populations. For example, sort cells according to biomarker intensity and immediately locate cells with highest intensity in the image. Just imagine the endless possibilities.
EXTENSIVE ANALYTICS
HALO reports morphological and multiplexed expression data on a cell-by-cell basis across entire tissue sections and maintains an interactive link between cell data and cell image. Click on any cell in the image and immediately see analysis outputs for that specific cell. Sorting and filtering capabilities allow the user to mine millions of cells while visually assessing corresponding cell populations. For example, sort cells according to biomarker intensity and immediately locate cells with highest intensity in the image. Just imagine the endless possibilities.
SCALABLE PLATFORM
HALO’s modular design and flexible licensing allow it to be adapted to any requirement and budget. Start with all the modules or just a few and add more modules as your needs change. Use HALO on a single workstation or ramp up to a server-based license for your entire group. Purchase a permanent license or renew annually.
Discover
HALO Modules
Click on the links below to explore our most popular HALO modules & add-ons. Use the category filters or click the 'Load More' button at the bottom of the list to view additional options.
Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.
Highplex FL
Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.
Multiplex IHC
Click here to fill out a demo request form. Upload up to three images if you would like to test analysis on your own slides.
Upload Images
Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjuction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.
Tissue Classifier Add-on
Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.
Spatial Analysis
Quantify spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH (RNAScope®)
Quantify IHC positivity and spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH-IHC (RNAScope®)
Quantify an unlimited number of fluorescent RNA/DNA probes and IF protein biomarkers on a per cell basis. Includes support for fluorescent RNAscope® assays.
FISH-IF (RNAScope®)
Quantify number, intensity and co-expression of an unlimited number of fluorescently labeled RNA or DNA probes on a per cell basis. Includes support for fluorescent RNAscope ® assays.
Multiplex FISH (RNAScope®)
Tissue Microarray (TMA) Add-on
Deconvolve up to five colors in brightfield and measure positive area and average optical density for each stain and stain colocalization (where applicable).
Area Quantification
Quantify expression of up to four brightfield cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear IHC
Measure the positive area, average intensity and dye colocalization (where applicable) of an unlimited number of fluorescent dyes.
Area Quantification FL
Quantify expression of an unlimited number of fluorescently labeled cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear FL
Quantify intensity of brightfield membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane IHC
Quantify intensity of fluorescently-labeled membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane FL
Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization
Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.
Vacuole
Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization FL
Quantifies area, diameter, and perimeter of muscle fibers stained with laminin or other fiber membrane stain.
Muscle Fiber
Quantifies area, diameter, and perimeter of fluorescently-labelled muscle fibers stained with membrane markers, such as laminin.
Muscle Fiber FL
Count pancreatic islets, quantify number of alpha and beta cells in each islet, and determine area of islets stained with insulin and glucagon or other islet-specific stains
Islet IHC
Count pancreatic islets fluorescently-labeled with up to three dyes, quantify number of dye positive cells per islet and area of dye positivity per islet.
Islet FL
Microglial Activation
Amplification & Deletion FISH
Analyze serially stained tissue sections stained with different markers or a single tissue section which has been stained, stripped and re-stained for multiple markers.
Serial Section Analysis Add-on
Discover HALO Modules
Click on the links below to explore our most popular HALO modules & add-ons. Use the category filters or click the 'Load More' button at the bottom of the list to view additional options.
Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.
Highplex FL
Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.
Multiplex IHC
Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjuction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.
Tissue Classifier Add-on
Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.
Spatial Analysis
Quantify spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH (RNAScope®)
Quantify IHC positivity and spot number, optical density and co-expression of one or two brightfield RNA or DNA probes on a per cell basis. Includes support for brightfield RNAscope® assays.
ISH-IHC (RNAScope®)
Quantify an unlimited number of fluorescent RNA/DNA probes and IF protein biomarkers on a per cell basis. Includes support for fluorescent RNAscope® assays.
FISH-IF (RNAScope®)
Quantify number, intensity and co-expression of an unlimited number of fluorescently labeled RNA or DNA probes on a per cell basis. Includes support for fluorescent RNAscope ® assays.
Multiplex FISH (RNAScope®)
Tissue Microarray (TMA) Add-on
Deconvolve up to five colors in brightfield and measure positive area and average optical density for each stain and stain colocalization (where applicable).
Area Quantification
Quantify expression of up to four brightfield cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear IHC
Measure the positive area, average intensity and dye colocalization (where applicable) of an unlimited number of fluorescent dyes.
Area Quantification FL
Quantify expression of an unlimited number of fluorescently labeled cytoplasmic/nuclear markers on a per cell basis and bin cells according to intensity (1+, 2+ and 3+).
Cytonuclear FL
Quantify intensity of brightfield membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane IHC
Quantify intensity of fluorescently-labeled membrane markers on a per cell basis and bin cells according to intensity (0, 1+, 2+, 3+).
Membrane FL
Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization
Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.
Vacuole
Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.
Object Colocalization FL
Quantifies area, diameter, and perimeter of muscle fibers stained with laminin or other fiber membrane stain.
Muscle Fiber
Quantifies area, diameter, and perimeter of fluorescently-labelled muscle fibers stained with membrane markers, such as laminin.
Muscle Fiber FL
Count pancreatic islets, quantify number of alpha and beta cells in each islet, and determine area of islets stained with insulin and glucagon or other islet-specific stains
Islet IHC
Count pancreatic islets fluorescently-labeled with up to three dyes, quantify number of dye positive cells per islet and area of dye positivity per islet.
Islet FL
Microglial Activation
Amplification & Deletion FISH
Analyze serially stained tissue sections stained with different markers or a single tissue section which has been stained, stripped and re-stained for multiple markers.
Serial Section Analysis Add-on
Podcasts
Check out the latest National Society for Histotechnology HistoHelp podcast with Dave Krull and Adam Smith!
Click the tab on the below to listen!
HistoHelp Podcasts: Listen Now
Platform Compatibility
HALO is compatible with a broad spectrum of image and digital slide formats. Yours not on the list? Email us your requirements.
File Formats
Non-proprietary (JPG, TIF, OME.TIFF)
Nikon (ND2)
3D Histech (MRXS)
Akoya (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Ventana (BIF)
Philips (iSyntax)
KFBIO (KFB)
DICOM (DCM*)
*whole-slide images
HALO Application Notes
Follow the links below to explore a few of the most popular HALO applications.
Fluorescence
Analyze Multiplexed Fluorescent Biomarkers in Whole Slide PerkinElmer Vectra® Polaris images fused in HALO
We demonstrate how HALO can create a multispectral 7-plex whole slide image from individual field-of-view image tiles captured from the PerkinElmer Vectra 3 or Polaris platform and analyze these images using our highplex FL module and spatial analysis modules.
Read More
Brightfield
Measure immune cell density and spatial distance to tumor cells within the tumor and invasive margin
Using our multiplex IHC module, we quantify immune cells and tumor cell markers across whole slide images. The tissue classifier add-on is employed to segment tumor and stroma and the spatial analysis module is used for proximity and invasive margin analysis.
Read More
Brightfield and Fluorescence
Quantify Single and Multiplexed RNAscope® probes across whole slide tissues
Using our ISH module for brightfield and our FISH module for fluorescence, we demonstrate how to quantify ISH signal from RNAScope® assays on a per cell or per unit area basis and output an overall expression score based on ACD Bio’s recommended scoring guidelines.
Read More
Read what our customers are publishing
Our customers are making vital discoveries in oncology, neuroscience, and diabetes research. Check out some of the research our customers are publishing using the HALO Image Analysis Platform and Indica analysis modules.
Click the button above to check out the latest high-impact publications where HALO image analysis was used.
Top Notch Training & Support
We’ve worked hard to ensure our HALO platform interfaces are the most intuitive in the industry, but we realize that you may still need extra support. We’ve got you covered!
Unlimited IT support, scientific support & training are included with every license at no additional cost. With support staff in the US, UK and Japan, you are supported in any time zone. Need help tuning an algorithm or training for new staff – we are just a call away.
The vast majority of our new customers heard about Indica Labs from an existing customer. The two primary reasons for referral – our user-friendly technology and excellent support. We know that you have a choice in image analysis providers and we strive to make sure every customers is 100% satisfied.
Top Notch Training & Support
We’ve worked hard to ensure our HALO platform interfaces are the most intuitive in the industry, but we realize that you may still need extra support. We’ve got you covered!
Unlimited IT support, scientific support & training are included with every license at no additional cost. With support staff in the US, UK and Japan, you are supported in any time zone. Need help tuning an algorithm or training for new staff – we are just a call away.
The vast majority of our new customers heard about Indica Labs from an existing customer. The two primary reasons for referral – our user-friendly technology and excellent support. We know that you have a choice in image analysis providers and we strive to make sure every customers is 100% satisfied.
Want to see more?
Fill out the form below to request a recorded or live demo of HALO or any of our other products or services.
You can also drop us an email at info@indicalab.com
We highly recommend using Google Chrome when filling out the info above. Please email marketing@indicalab.com for support.