In the most recent HALO AI 3.2 release, we introduced two powerful neural networks for nuclear segmentation and phenotyping. In this 60-minute presentation, Switzerland-based Application Scientist, Dr. Ghislaine Lioux, will demonstrate how to set-up and train HALO AI Nuclei Segmenter and Nuclei Phenotyper, show how to apply Nuclei Segmenter within HALO’s cell-based algorithms, provide helpful tips to speed up the creation of AI training sets, and discuss common applications for each neural network, such as segmentation of highly clustered immune cell populations, segmentation of over-digested nuclei in RNAscope assays, PD-L1 cell phenotyping, and tumor and immune cell detection on H&E images.