Photo credit: Mélanie Beaulande, PhD and Angela Ramos, MSc of Roche Diagnostics

Masterclass Webinar 1: Optimizing a 3-plex Multiplex IHC Assay Using Roche Discovery Reagents and HALO® Image Analysis

Date: 11 May 2023
Time: 8:00 – 9:00 PST | 11:00 – 12:00 EST | 16:00 – 17:00 GMT
Location: Webinar

Learn how to optimize a Roche Discovery 3-plex assay from staining to image analysis


In this 60-minute webinar, we will discuss best practices and optimization techniques for Roche Discovery multiplex immunohistochemical (mIHC) assays and HALO® image analysis. First, we will present methods, tips and tricks to design and optimize staining protocols. Next, we will share a series of mIHC assays where various chromogen combinations were tested on an assay comprised of three biomarkers and hematoxylin. From this dataset, we will discuss which assays are appropriate for image analysis with the HALO® Multiplex IHC module, which are not, and why.

Learning Objectives

  • Advance your mIHC assay design and optimization knowledge 
  • Improve your brightfield image deconvolution for mIHC analysis with tips and tricks 
  • Learn from examples of successful 3-plex chromogenic image analysis as well as images that are problematic and learn from common challenges 


Mélanie Beaulande, PhD 
Scientific Expert Discovery & Multiplexing EMEA – LATAM 
Roche Diagnostics 

Mélanie is a Scientific Expert in Discovery at Roche Diagnostics France. She holds a Ph.D. and has been working for Ventana/Roche for 18 years. Her work is dedicated to the Discovery business which is the Research part of Roche Tissue Diagnosis. Her main focus is Multiplexing assay development and its translational application from research to clinical purposes.

Angela Ramos, MSc.
Application Project Manager 
Roche Diagnostics 

Angela Ramos is working as an Application Product Manager, with the DISCOVERY ULTRA instrument supporting the customers within the EMEA LATAM region in Roche Tissue Diagnostics. She graduated with a Biotechnology Engineering degree complemented by a Masters in Biology; The experience she acquired before joining Roche and after as a field based role, gave her the flexibility and adaptability to the different requests and demands over her latest managing position. What she enjoys the most is the openness and diversity while working with the DISCOVERY portfolio, exploring all possibilities especially within the Multiplexing applications.


Kim Collins, PhD 
Sr. Marketing Manager 
Indica Labs 

Kim completed her undergraduate degree in Biochemistry and Cell Biology at the University of California, San Diego, and obtained her PhD from the University of Washington and Fred Hutchinson Cancer Research Center Molecular and Cellular Biology Program where she developed a passion for microscopy. In her postdoctoral fellowship at the Stowers Institute of Medical Research, she continued to use high and super resolution microscopy to advance her research. Kim has 10 years of experience working on product and marketing teams at companies focused on microscopy and image analysis. In her current role, she leads a team of three and manages comarketing partnerships, creation of print and digital scientific and clinical marketing content, and events supporting Indica Labs’ products and services.


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