Masterclass Webinar: Optimizing RNAscope Image Analysis
Masterclass Webinar: Optimizing RNAscope Image Analysis
Date: 05 May 2022
Time: 8:00 – 9:00 PDT | 11:00 – 12:00 EDT | 16:00 – 17:00 BST
Location: Webinar
Thoughtful RNAscope experimental design combined with rigorous assay execution and validation enables accurate quantification of spots and protein co-detection with the HALO image analysis platform in brightfield and fluorescence assays.
Summary
In this 60-min webinar, Dr. Ghislaine Lioux will present solutions to common RNAscope image analysis challenges including how to optimize color deconvolution, nuclear segmentation, and how to manage spot clusters. A recommended end-to-end workflow, from selecting an imaging modality to validating image analysis results will be presented. Join us for a live demonstration of HALO® image analysis on optimization of in situ hybridization (ISH) analysis, fluorescence in situ hybridization (FISH) analysis, and protein/ nucleic acid co-detection assay.
learn
- How to address common image analysis challenges in RNAscope assays
- How to optimize HALO image analysis settings for ISH, FISH, and codetection assays such as ISH-IHC and FISH-IF
- About the checkpoints for RNAscope quantitative image analysis success
Presenters
