In this study, researchers set out to understand how miR-126-3, a microRNA found at increased levels in offspring of maternally obese mice, functioned in adipocyte metabolism. de Almeida-Faria and colleagues used proteomic approaches to identify a novel ER protein that is a direct target of miR-126-3 called Lunapark. HALO and HALO AI were used to train a DenseNet algorithm to selectively identify crown-like structures in H&E-stained fat tissue. Further, de Almeida-Faria and colleagues demonstrate that maternal obesity in mice leads to an increased risk of type 2 diabetes in offspring by targeting miR-126-3 regulation. Therefore, miR-126-3 is identified as a potential therapeutic target that could impact obesity and type 2 diabetes.