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Tumor-specific tumor-resident cytotoxic T cells predict recurrence in stage III melanoma patients treated with adjuvant immunotherapy

Tumor-specific tumor-resident cytotoxic T cells predict recurrence in stage III melanoma patients treated with adjuvant immunotherapy

20 January 2022
2:00pm – 3:00pm PST | 5:00pm- 6:00pm EST

 

21 January 2022
9:00AM – 10:00am AEDT 

LEARNING OBJECTIVES

  • Learn how cellular phenotyping combined with spatial analysis provides insights into stratifying melanoma patient populations
  • Learn which cytotoxic T-cell populations are predictive of recurrence in anti-PD-1 treated high risk state III melanoma patients
  • Learn how HALO and HALO AI collaborate to perform tissue and cell segmentation, quantification of T-cell subsets in tumor regions, and spatial analysis

This webinar is ideal for cancer researchers, pathologists, attending physicians, image analysts, and others working in digital pathology.

ABSTRACT

Adjuvant immune-checkpoint inhibitor (ICI) immunotherapies have significantly reduced the recurrence rate in high-risk stage-III melanoma patients compared to surgery alone. However, 48% of treated patients will develop recurrent disease within 4 years. There is a need to identify predictive biomarkers of recurrence after adjuvant ICI, to enable identification of patients in need of alternative treatment strategies. As cytotoxic T-cells are critical for the anti-tumor response to ICI, we sought to determine whether specific subsets were predictive of recurrence in anti-PD-1-treated high-risk stage III patients. Stage III melanoma tumor tissue taken prior to adjuvant anti-PD-1 therapy ± anti-CTLA-4 were analyzed with Opal multiplex immunohistochemistry to quantify CD8+ T cell phenotypes using the markers CD39, CD103 and PD-1. Associations with recurrence were sought in a discovery cohort (N = 103) and assessed in a validation cohort (N  = 33). Image analysis was performed on the whole slide scans to stitch regions together, perform tissue and cell segmentation, quantify T-cells subsets within the tumor region and perform spatial analysis in relation to melanoma cells (HALO and HALO AI, Indica Labs).

Two CD8+ T cell subpopulations were significantly associated with recurrence disease recurrence on adjuvant immunotherapies.  First, tumor-specific, resident T-cells (Tsrm) comprised a significantly higher proportion of CD8+ T cells in recurrence-free patients (P = 0.0004). Conversely, bystander T-cells comprised a significantly greater proportion of T-cells in patients who developed recurrence (P = 0.0002). Spatial analysis identified that Tsrm localized significantly closer to melanoma cells than bystander T-cells. Multivariable analysis confirmed that low Tsrm proportions and other clinical factors were associated with recurrence. The final Cox regression model identified patients who developed recurrence with an AUC of 75.9% in the discovery cohort and AUC of 69.5% in a separate validation cohort.

Pre-treatment Tsrm cells are strongly associated with a reduced risk of melanoma recurrence in adjuvant-PD-1-treated patients. This population may not only represent a biomarker for recurrence following anti-PD-1 therapy but also be an avenue for therapeutic manipulation and enhancing outcomes for cancer patients treated with immunotherapy.

PRESENTERS

James Wilmott, PhD
NHMRC investigator fellow | Senior scientist
The Melanoma Institute Australia, The University of Sydney      

Senior lecturer James Wilmott is the research leader of the Translational Melanoma Research Group of the Melanoma Institute Australia, The University of Sydney. Dr Wilmott is a current holder of an NHMRC early career fellowship and received his PhD in 2013 from the University of Sydney. Dr Wilmott is recognized internationally as an emerging expert in clinical implications of genomic profiles (First author Nature and Int J Cancer), immune profiling of cancer tissue (Co-author in Nature, The Lancet Oncology, senior author in Clinical Cancer Research and Oncoimmunology) and in biomarkers of response of systemic therapies (Senior author Cancer Cell). Dr Wilmott has amassed over 169 publications since 2016 in the top medical journals, including: twice in Nature, twice in Nature Cancer, once in Cell, twice in Lancet Oncology, once in Cancer Cell and eleven times in Clinical Cancer Research and six times in Nature communications. With over 18,230 citations, with 43 articles with >100 citations and a H-index of 56. As a translational research scientist, Dr Wilmott has worked closely with clinical groups to identify areas of need, find a solution and work to implement findings into improved patient outcomes.