The accumulation of aggregated tao proteins in neurons is a hallmark of several neurodegenerative diseases, including Alzheimer’s disease. A challenge in the study of tauopathies has been the lack of an appropriate model system. He and colleagues demonstrate here a new mouse model for human tauopathies where the expression of tao isoforms is similar to that in human adult brains. The Area Quantification Module of HALO played a key role in the analysis of immunohistochemistry on mouse neuronal tissue, including the analysis of tao, neurofilament light chain, and MAP2. This important study will pave the way for future studies addressing the molecular and pathological mechanisms of the development of a class of devastating neurological diseases.