HALO 4.4 introduces major improvements to fluorescent channel management, quality control, performance, and everyday workflows. Users have improved control of complex fluorescent images with expanded channel management. Search and sort channels, create and save channel groups, and fine-tune black-in and white-in values directly from the expanded channel list. With Autofit available right where you need it, optimizing image display takes fewer clicks. 

The new Object Data Gallery makes reviewing results more intuitive. You can quickly inspect randomly selected objects alongside their associated data values, streamlining quality control and helping spot issues faster. 

HALO 4.4 also delivers a smoother experience with faster annotation, quicker object data loading, and improved image viewer performance. Analysis data can also be moved more easily with new interoperability features, including registration transform file export and GeoJSON annotations compatible with the Xenium analysis platform. 

The latest version makes common tasks simpler with quality-of-life improvements including the ability to edit image paths when files are moved or renamed on disk, jump directly to a study from the Image tab, search and batch delete TMA blocks, and access more detailed system information and export HALOinformation.json. 

Lastly, HALO 4.4 introduces updates to the Multiplex IHC and Highplex FL modules, including adjustable membrane thickness and the ability to control post-processing on the module side for embedded AI segmentation modules. Additional enhancements include Threshold Assist compatibility for brightfield images and new object data outputs for nuclear and cellular morphology, signal OD/intensity heterogeneity, and Haralick texture features.