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Axon Quantification Module

Detect and measure axons and myelin areas with the HALO® Axon Quantification module. For use on brightfield images of stained nerve cross sections, this module reports the inner, outer, and myelin areas, inner and outer diameters, and G-Ratio for each axon, and provides both summary and detailed per-axon metrics. See the big picture and quickly spot outliers thanks to the module’s automatically generated histograms of axon G-Ratios and inner and outer diameters.

Try it out! Click here to initiate your free proof-of-concept HALO image analysis.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • KFBIO (KFB, KFBF)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Axon Quantification module. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Intrathecal Administration of AAV/GALC Vectors in 10- 11-Day-Old Twitcher Mice Improves Survival and Is Enhanced by Bone Marrow Transplant Karmuthil-Melethil S, Marshall MS, Heindel C, Jakubauskas B, Bongarzone ER, Gray SJ2016Journal of Neuroscience ResearchNeuroscienceAxon
Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury Stewart A, Glaser E, Mott C, Bailey W, Sullivan P, Patel S, Gensel J
2022Journal of Neurotrauma
Neuroscience
Axon
Sensory neurons display cell-type-specific vulnerability to loss of neuron-glia interactionsElbaz B, Yang L, Vardy M, Isaac S, Rader B, Kawaguchi R, Traka M, Woolf C, Renthal W, Popko B2022Cell ReportsNeuroscienceAxon
Combination of pathological, biochemical and behavioral evaluations for peripheral neurotoxicity assessment in isoniazid-treated ratsKashimura A, Nishikawa S, Ozawa Y, Hibino Y, Tateoka T, Mizukawa M, Nishina H, Sakairi T, Shiga T, Aihara N, Kamiie J2023Journal of Toxicologic PathologyToxicologyAxon

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Simultaneously analyze an unlimited number of fluorescent dyes and measure object density, area, diameter, and intensity, as well as colocalizations, if applicable.

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Microglial Activation

Quantify microglial activation based on length and thickness of microglial processes.

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Use the arrows above to view additional related modules

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