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HALO® Module

Axon Quantification

The Axon module counts and measures axons and myelin areas in nerve cross-sections. Since axon cross-sections are irregularly shaped, the algorithm uses image processing techniques to detect each axon’s minimum and median diameter. Once each axon has been measured the software generates important statistics including the average inner and outer diameters, average G-Ratio, average perimeter, total axon area, total myelin area, and average myelin thickness. Additionally, it produces several histograms including an axon G-Ratio histogram.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • KFBIO (KFB, KFBF)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Axon Quantification module. 

Your publication not on the list?  Drop us an email to let us know about it!

Title Authors Year Journal Application HALO Modules
Intrathecal Administration of AAV/GALC Vectors in 10- 11-Day-Old Twitcher Mice Improves Survival and Is Enhanced by Bone Marrow Transplant Karmuthil-Melethil S, Marshall MS, Heindel C, Jakubauskas B, Bongarzone ER, Gray SJ 2016 Journal of Neuroscience Research Neuroscience Axon
Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury Stewart A, Glaser E, Mott C, Bailey W, Sullivan P, Patel S, Gensel J
2022 Journal of Neurotrauma
Neuroscience
Axon
Sensory neurons display cell-type-specific vulnerability to loss of neuron-glia interactions Elbaz B, Yang L, Vardy M, Isaac S, Rader B, Kawaguchi R, Traka M, Woolf C, Renthal W, Popko B 2022 Cell Reports Neuroscience Axon
Combination of pathological, biochemical and behavioral evaluations for peripheral neurotoxicity assessment in isoniazid-treated rats Kashimura A, Nishikawa S, Ozawa Y, Hibino Y, Tateoka T, Mizukawa M, Nishina H, Sakairi T, Shiga T, Aihara N, Kamiie J 2023 Journal of Toxicologic Pathology Toxicology Axon

Related HALO Modules

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Simultaneously analyze an unlimited number of fluorescent dyes and measure object density, area, diameter, and intensity, as well as colocalizations, if applicable.

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Microglial Activation

Quantify microglial activation based on length and thickness of microglial processes.

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Use the arrows above to view additional related modules

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