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Microglial Activation Module​

Measure microglia cell activation in brightfield images with the HALO® Microglial Activation module. This module detects microglia, soma, and processes, and reports the number of activated and inactivated microglial cells and the number of non-microglia cells detected. The tool also precisely measures cell processes, reporting the total process area and length as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points and end points per cell. Leverage optional interactive markup images to dynamically explore your results by toggling markup of activated and inactivated microglia, microglial processes and skeleton, branch and end points, and microglia radius.

Try it out! Click here to initiate your free proof-of-concept HALO image analysis.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Microglial Activation modules. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO ModulesProduct
Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T2018Neurobiology of AgingNeuroscienceArea Quantification, Object Colocalization, MicrogliaHALO
Diverse human astrocyte and microglial transcriptional responses to Alzheimerís pathologySmith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P2021Acta NeuropathologicaNeuroscienceArea Quantification, Multiplex IHC, MicrogliaHALO
T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of†?-Synuclein FibrilsGeorge S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P2021Journal of Parkinson's DiseaseImmunology, NeuroscienceMicrogliaHALO
Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in DementiaNaseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M2022BiomoleculesNeuroscienceMicrogliaHALO
Association between APOE genotype and microglial cell morphologyKloske C, Gearon M, Weekman E, Rogers C, Patel E, Bachstetter A, Nelson P, Wilcock D2023Journal of Neuropathology & Experimental NeurologyneuroscienceObject Colocalization, MicrogliaHALO
Characterization of Traumatic Brain Injury in a Gyrencephalic Ferret Model Using the Novel Closed Head Injury Model of Engineered Rotational Acceleration (CHIMERA)Krieg J, Leonard A, Tuner R, Corrigan F2023Neurotrauma ReportsNeuroscienceMicrogliaHALO
Anatomical distribution of Fyn kinase in the human brain in Parkinson's diseaseGuglietti B, Mustafa S, Corrigan F, Collins-Praino LE2023Parkinsonism & Related DisordersNeuroscienceMicrogliaHALO
Female mice display sex-specific differences in cerebrovascular function and subarachnoid haemorrhage-induced injuryDinh DD, Wan H, Lidington D, Bolz SS2024EBioMedicine NeuroscienceMicrogliaHALO

Related HALO Modules

Object Colocalization

Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Object Colocalization FL

Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Microglial Activation FL

Quantify microglial activation in fluorescence based on detection of microglia, soma, and processes, by counting branch points, and by determining area, length, and thickness of processes.

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