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HALO® Module

Microglial Activation

The Microglial Activation module measures microglia cell activation in brightfield images. This module detects microglia, soma, and processes, and ultimately reports the number of activated microglial cells, the number of inactivated microglial cells, and the number of negative (non-microglia) cells detected. In addition to cell classification and counting, this tool precisely measures cell processes. It reports the total process area and length, as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points and end points per cell. The interactive markup output option enables users to easily interpret and interrogate data.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Microglial Activation modules. 

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TitleAuthorsYearJournalApplicationHALO ModulesProduct
Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T2018Neurobiology of AgingNeuroscienceArea Quantification, Object Colocalization, MicrogliaHALO
Diverse human astrocyte and microglial transcriptional responses to Alzheimerís pathologySmith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P2021Acta NeuropathologicaNeuroscienceArea Quantification, Multiplex IHC, MicrogliaHALO
T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of†?-Synuclein FibrilsGeorge S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P2021Journal of Parkinson's DiseaseImmunology, NeuroscienceMicrogliaHALO
Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in DementiaNaseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M2022BiomoleculesNeuroscienceMicrogliaHALO
Association between APOE genotype and microglial cell morphologyKloske C, Gearon M, Weekman E, Rogers C, Patel E, Bachstetter A, Nelson P, Wilcock D2023Journal of Neuropathology & Experimental NeurologyneuroscienceObject Colocalization, MicrogliaHALO

Related HALO Modules

Object Colocalization FL

Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Object Colocalization

Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

Learn More
Microglial Activation FL

Quantify microglial activation in fluorescence based on detection of microglia, soma, and processes, by counting branch points, and by determining area, length, and thickness of processes.

Learn More

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