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HALO® Module

Muscle Fiber FL

The Muscle Fiber FL module detects fluorescently-stained muscle fibers and reports the total count as well as the perimeter, area, and diameter of each fiber. The module also detects nuclei (and nuclear position with respect to the fiber) and reports fiber membrane dye intensity and co-localization (where applicable).

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Muscle Fiber and Muscle Fiber FL modules. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
The Goto Kakizaki rat: Impact of age upon changes in cardiac and renal structure, functionMeagher P, Civitarese R, Lee X, Gordon M, Bugyei-Twum A, Desjardins J, Kabir G, Zhang Y, Kosanam H, Visram A, Leong-Poi H, Advani A, Connelly K2021PLOS ONEMyologyMuscle Fiber
A growth factor–expressing macrophage subpopulation orchestrates regenerative inflammation via GDF-15Patsalos A, Halasz L, Medina-Serpas M, Berger W, Daniel B, Tzerpos P, Kiss M, Nagy G, Fischer C, Simandi Z, Varga T, Nagy L2021Journal of Experimental MedicineMuscle Fiber
Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in miceKenjo E, Hozumi H, Makita Y, Iwabuchi K, Fujimoto N, Matsumoto S, Kimura M, Amano Y, Ifuku M, Naoe Y, Inukai N, Hotta A2021Nature CommunicationsOtherMuscle Fiber
Inhibition of myostatin prevents microgravity-induced loss of skeletal muscle mass and strengthSmith RC, Cramer MS, Mitchell PJ, Lucchesi J, Ortega AM, Livingston EW, Ballard D, Zhang L, Hanson J, Barton K, Berens S, Credille KM, Bateman TA, Ferguson VL, Ma YL, Stodieck LS2020PLOS OneMyologyMuscle Fiber
Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration Giannakis N, Sansbury BE, Patsalos A, Hays TT, Riley CO, Han X, Spite M, Nagy L2019Nature ImmunologyImmunology, MyologyMuscle Fiber
Development of antibody-siRNA conjugate targeted to cardiac and skeletal muscles Sugo T, Terada M, Oikawa T, Miyata K, Nishimura S, Kenjo E, Ogasawara-Shimizu M, Makita Y, Imaichi S, Murata S, Kentaro Otaka, Kikuchi K, Teratani M, Masuda Y, Kamei T, Takagahara S, Ikeda S, Ohtaki T, Matsumoto H2016The Journal of Controlled ReleaseMyologyCytonuclear, Muscle Fiber
Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky TB, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy AJ, Yancopoulos GD, Gromada J, Stitt T2015Skeletal MuscleMyologyMuscle Fiber
Peripheral androgen receptor gene suppression rescues disease in mouse models of spinal and bulbar muscular atrophy Lieberman AP, Yu Z, Murray S, Peralta R, Low A, Guo S, Yu XX, Cortes CJ, Bennett CF, Monia BP, La Spada AR, Hung G2014Cell ReportsMyologyMuscle Fiber

Related HALO Modules

Tissue Classifier Add-On

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Muscle Fiber

Quantifies area, diameter, and perimeter of muscle fibers stained with laminin or other fiber membrane stain.

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Highplex FL

Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.

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