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HALO® Module

Muscle Fiber

The Muscle Fiber module detects fibers and reports the total count of muscle fibers as well as the perimeter, area, and diameter of each fiber.

Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally the software reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Muscle Fiber and Muscle Fiber FL modules. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO ModulesProduct
Inhibition of myostatin prevents microgravity-induced loss of skeletal muscle mass and strengthSmith RC, Cramer MS, Mitchell PJ, Lucchesi J, Ortega AM, Livingston EW, Ballard D, Zhang L, Hanson J, Barton K, Berens S, Credille KM, Bateman TA, Ferguson VL, Ma YL, Stodieck LS2020PLOS OneMyologyMuscle FiberHALO
Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration Giannakis N, Sansbury BE, Patsalos A, Hays TT, Riley CO, Han X, Spite M, Nagy L2019Nature ImmunologyImmunology, MyologyMuscle FiberHALO
Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky TB, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy AJ, Yancopoulos GD, Gromada J, Stitt T2015Skeletal MuscleMyologyMuscle FiberHALO
Peripheral androgen receptor gene suppression rescues disease in mouse models of spinal and bulbar muscular atrophy Lieberman AP, Yu Z, Murray S, Peralta R, Low A, Guo S, Yu XX, Cortes CJ, Bennett CF, Monia BP, La Spada AR, Hung G2014Cell ReportsMyologyMuscle FiberHALO
The Goto Kakizaki rat: Impact of age upon changes in cardiac and renal structure, functionMeagher P, Civitarese R, Lee X, Gordon M, Bugyei-Twum A, Desjardins J, Kabir G, Zhang Y, Kosanam H, Visram A, Leong-Poi H, Advani A, Connelly K2022PLOS ONEMyologyMuscle FiberHALO
A growth factorñexpressing macrophage subpopulation orchestrates regenerative inflammation via GDF-15Patsalos A, Halasz L, Medina-Serpas M, Berger W, Daniel B, Tzerpos P, Kiss M, Nagy G, Fischer C, Simandi Z, Varga T, Nagy L2021Journal of Experimental MedicineMuscle FiberHALO
Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in miceKenjo E, Hozumi H, Makita Y, Iwabuchi K, Fujimoto N, Matsumoto S, Kimura M, Amano Y, Ifuku M, Naoe Y, Inukai N, Hotta A2021Nature CommunicationsOtherMuscle FiberHALO
Electrical impedance myography detects dystrophin-related muscle changes in mdx miceHiyoshi T, Zhao F, Baba R, Hirakawa T, Kuboki R, Suzuki K, Tomimatsu Y, O'Donnell P, Han S, Zach N, Nakashima M2023Research SquareMyologyArea Quantification, Muscle FiberHALO
Effects of the purified dry extract of fermented ginseng BST204 on muscle fiber regenerationJo S, Park Y, Chang Y, Moon J, Lee S, Lee H, Kim M, Kim D, Bae S, Park S, Yun H, You J, Im M, Han H, Kim S, Jin D2023Biochemistry and Biophysics ReportsMyologyMuscle FiberHALO
Rotator cuff muscle fibrosis can be assessed using ultrashort echo time magnetization transfer MRI with fat suppressionChang EY, Suprana A, Tang Q, Cheng X, Fu E, Orozco E, Jerban S, Shah S, Du J, Ma Y2023NMR in BiomedicineFibrosisMuscle FiberHALO
Rotator cuff muscle fibrosis can be assessed using ultrashort echo time magnetization transfer MRI with fat suppressionChang EY, Suprana A, Tang Q, Cheng X, Fu E, Orozco E, Jerban S, Shah S, Du J, Ma Y2023NMR in BiomedicineFibrosisMuscle FiberHALO
Peripheral neural interfaces: Skeletal muscles are hyper-reinnervated according to the axonal capacity of the surgically rewired nervesTereshenko V, Dotzauer D C, Schmoll M, Harnoncourt L, Carrero Rojas G, Gfrerer L, Eberlin K R, Austen Jr. W G, Blumer R, Farina D, Aszmann O C2024Science AdvancesNeuroscienceMuscle FiberHALO

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Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Quantify positive fiber or membrane staining for an unlimited number of channels.

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