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Muscle Fiber Module

Detect and measure muscle fibers in brightfield images with the HALO® Muscle Fiber module. This module detects up to three stains—a nuclear, membrane, and optional fiber stain—and reports the total fiber count as well as the average fiber diameter, perimeter length, and area. Perform in-depth analysis of individual fiber measurements with optional per-object results, including the minimum and maximum diameter, perimeter length, area, and presence/absence of a central nucleus for each fiber.

Try it out! Click here to initiate your free proof-of-concept HALO image analysis.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Muscle Fiber and Muscle Fiber FL modules. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO ModulesProduct
Inhibition of myostatin prevents microgravity-induced loss of skeletal muscle mass and strengthSmith RC, Cramer MS, Mitchell PJ, Lucchesi J, Ortega AM, Livingston EW, Ballard D, Zhang L, Hanson J, Barton K, Berens S, Credille KM, Bateman TA, Ferguson VL, Ma YL, Stodieck LS2020PLOS OneMyologyMuscle FiberHALO
Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration Giannakis N, Sansbury BE, Patsalos A, Hays TT, Riley CO, Han X, Spite M, Nagy L2019Nature ImmunologyImmunology, MyologyMuscle FiberHALO
Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky TB, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy AJ, Yancopoulos GD, Gromada J, Stitt T2015Skeletal MuscleMyologyMuscle FiberHALO
Peripheral androgen receptor gene suppression rescues disease in mouse models of spinal and bulbar muscular atrophy Lieberman AP, Yu Z, Murray S, Peralta R, Low A, Guo S, Yu XX, Cortes CJ, Bennett CF, Monia BP, La Spada AR, Hung G2014Cell ReportsMyologyMuscle FiberHALO
The Goto Kakizaki rat: Impact of age upon changes in cardiac and renal structure, functionMeagher P, Civitarese R, Lee X, Gordon M, Bugyei-Twum A, Desjardins J, Kabir G, Zhang Y, Kosanam H, Visram A, Leong-Poi H, Advani A, Connelly K2022PLOS ONEMyologyMuscle FiberHALO
A growth factorñexpressing macrophage subpopulation orchestrates regenerative inflammation via GDF-15Patsalos A, Halasz L, Medina-Serpas M, Berger W, Daniel B, Tzerpos P, Kiss M, Nagy G, Fischer C, Simandi Z, Varga T, Nagy L2021Journal of Experimental MedicineMuscle FiberHALO
Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in miceKenjo E, Hozumi H, Makita Y, Iwabuchi K, Fujimoto N, Matsumoto S, Kimura M, Amano Y, Ifuku M, Naoe Y, Inukai N, Hotta A2021Nature CommunicationsOtherMuscle FiberHALO
Electrical impedance myography detects dystrophin-related muscle changes in mdx miceHiyoshi T, Zhao F, Baba R, Hirakawa T, Kuboki R, Suzuki K, Tomimatsu Y, O'Donnell P, Han S, Zach N, Nakashima M2023Research SquareMyologyArea Quantification, Muscle FiberHALO
Effects of the purified dry extract of fermented ginseng BST204 on muscle fiber regenerationJo S, Park Y, Chang Y, Moon J, Lee S, Lee H, Kim M, Kim D, Bae S, Park S, Yun H, You J, Im M, Han H, Kim S, Jin D2023Biochemistry and Biophysics ReportsMyologyMuscle FiberHALO
Rotator cuff muscle fibrosis can be assessed using ultrashort echo time magnetization transfer MRI with fat suppressionChang EY, Suprana A, Tang Q, Cheng X, Fu E, Orozco E, Jerban S, Shah S, Du J, Ma Y2023NMR in BiomedicineFibrosisMuscle FiberHALO
Rotator cuff muscle fibrosis can be assessed using ultrashort echo time magnetization transfer MRI with fat suppressionChang EY, Suprana A, Tang Q, Cheng X, Fu E, Orozco E, Jerban S, Shah S, Du J, Ma Y2023NMR in BiomedicineFibrosisMuscle FiberHALO
Peripheral neural interfaces: Skeletal muscles are hyper-reinnervated according to the axonal capacity of the surgically rewired nervesTereshenko V, Dotzauer D C, Schmoll M, Harnoncourt L, Carrero Rojas G, Gfrerer L, Eberlin K R, Austen Jr. W G, Blumer R, Farina D, Aszmann O C2024Science AdvancesNeuroscienceMuscle FiberHALO

Related HALO Module

Highplex FL

Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.

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Tissue Classifier Add-on

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Muscle Fiber FL

Quantify fiber or membrane positivity for an unlimited number of fluorescent dyes along with fiber diameter, perimeter, and area.

Learn More

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