Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane. With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue. It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya PhenoImager (Phenoptics), PhenoCycler (CODEX®), Vectra® 3, and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Lunaphore (OME.TIFF images from the COMET™ Platform), Ultivue (FixVUE™, FlexVUE™, U-Vue® Panels and InSituPlex® Technology), Standard BioTools (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).
File formats supported by the HALO image analysis platform:
- Non-proprietary (JPG, TIF, OME.TIFF)
- Nikon (ND2)
- 3D Histech (MRXS)
- Akoya (QPTIFF, component TIFF)
- Olympus / Evident (VSI)
- Hamamatsu (NDPI, NDPIS)
- Aperio (SVS, AFI)
- Zeiss (CZI)
- Leica (SCN, LIF)
- Ventana (BIF)
- Philips (iSyntax, i2Syntax)
- KFBIO (KFB, KFBF)
- DICOM (DCM*)
*whole-slide images
Qualitative and Quantitative Evaluation of the Tissue Micro-Environment by High-Resolution 17-Plex Immunofluorescence Reveals Distinct Populations
Check out this eBook to see how AI-based nuclear segmentation and tissue classification can streamline cell phenotyping across tissue compartments and samples.
Sequential Same Slide Multiplex IF and H&E Staining for Combined Phenotypic and Morphological Characterization of FFPE Tissue Sections
Read this eBook for an introduction to a sequential staining and image analysis workflow that leverages a terminal H&E stain for the delineation of tissue architectural regions in subsequent HALO image analysis.
Introduction of a Robust Workflow for the Whole-Slide Acquisition and Co-Registration of Multiplex Immunofluorescence Tissue Images for Analysis of a 9-Color, 8-Marker Immunophenotyping Assay
Download our eBook to learn about a HALO workflow for co-registration and cellular analysis of immunofluorescence images from cyclically stained and imaged slides.
Publication Spotlight
The table below includes publications that cite the Highplex FL module.
Your publication not on the list? Drop us an email to let us know about it!
| Title | Authors | Year | Journal | Application | HALO Modules | Product |
|---|---|---|---|---|---|---|
| Cannabinoid control of gingival immune activation in chronically SIV-infected rhesus macaques involves modulation of the indoleamine-2,3-dioxygenase-1 pathway and salivary microbiome | McDew-White M, Lee E, Alvarez X, Sestak K, Ling B, Byrareddy S, Okeoma C, Mohan M | 2022 | EBioMedicine | Infectious Disease | Area Quantification, Cytonuclear, Highplex FL | HALO |
| ACE2-IgG1 fusions with improved in vitro and in vivo activity against SARS-CoV-2 | Iwanaga N, Cooper L, Rong L, Maness N, Beddingfield B, Qin Z, Crabtree J, Tripp R, Yang H, Blair R, Jangra S, Garcia-Sastre A, Schotsaert M, Chandra S, Robinson J, Srivastava A, Rabito F, Qin X, Kolls J | 2022 | iScience | Infectious Disease | Highplex FL | HALO AI |
| Tumor MHC Class I Expression Associates with Intralesional Interleukin-2 Response in Melanoma | Pourmaleki M, Jones C, Ariyan C, Zeng Z, Pirun M, Navarrete D, Li Y, Zhang M, Nandakumar S, Campos C, Nadeem S, Klimstra D, Temple-Oberle C, Brenn T, Kipson E, Schenk K, Stein J, Taube J, White M, Traweek R, Wargo J, Kirkwood J, Gasmi B, Goff S, Corwin A, McDonough E, Ginty F, Callahan M, Schietinger A, Socci N, Mellinghoff I, Hollmann T | 2022 | Cancer Research Immunology | Immuno-oncology | Highplex FL | HALO |
| Progranulin mediates immune evasion of pancreatic ductal adenocarcinoma through regulation of MHCI expression | Cheung P, Yang J, Fang R, Borgers A, Krengel K, Stoffel A, Althoff K, Yip C, Siu E, Ng L, Lang K, Cham L, Engel D, Soun C, Cima I, Scheffler B, Striefler J, Sinn M, Bahra M, Pelzer U, Oettle H, Markus P, Smeets E, Aarntzen E, Savvatakis K, Liffers S, Lueong S, Neander C, Bazarna A, Zhang X, Paschen A, Crawford H, Chan A, Cheung S, Siveke J | 2022 | Nature Communications | Oncology | Cytonuclear, Spatial Analysis, Highplex FL | HALO |
| Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancer | Du S, Qian J, Tan S, Li W, Liu P, Zhao J, Zeng Y, Xu L, Wang Z, Cai J | 2022 | Cancer Letters | Oncology | Area Quantification, Cytonuclear, Spatial Analysis, Highplex FL | HALO |
| Understanding the impact of chemotherapy on the immune landscape of high-grade serous ovarian cancer | Vanguri R, Benhamida J, Young J, Li Y, Zivanovic O, Chi D, Snyder A, Hollmann T, Mager K | 2022 | Gynecologic Oncology Reports | Oncology | Classifier, Cytonuclear, Highplex FL | HALO |
| New Fully Automated Preparation of High Apparent Molar Activity 68Ga-FAPI-46 on a Trasis AiO Platform | Da Pieve C, Braga M, Turton D, Valla F, Cakmak P, Plate K, Kramer-Marek G | 2022 | Molecules | Other | Cytonuclear, Highplex FL | HALO |
| Retrospective analysis of the efficacy of adjuvant cytokine-induced killer cell immunotherapy combined with chemotherapy in colorectal cancer patients after surgery | Li X, Zhou H, Huang W, Wang X, Meng M, Hou Z, Liao L, Tang W, Xie Y, Wang R, Yu H, Wang L, Zhu H, Wang W, Tan J, Li R | 2022 | Clinical & Translational Immunology | Oncology | Cytonuclear, Highplex FL | HALO |
| Pancreas resident macrophage-induced fibrosis has divergent roles in pancreas inflammatory injury and PDAC | Baer J, Zuo C, Kang L, Alarcon de la Lastra A, Bocherding N, Knolhoff B, Bogner S, Zhu Y, Lewis M, Zhang N, Kim K, Fields R, Mills J, Ding L, Randolph G, DeNardo D | 2022 | bioRxiv | Fibrosis | Classifier, Area Quantification, Cytonuclear, Multiplex IHC, Highplex FL, Figure Maker | HALO |
| Genomic and single-cell landscape reveals novel drivers and therapeutic vulnerabilities of transformed cutaneous T-cell lymphoma | Song X, Chang S, Seminario-Vidal L, de Mingo Pulido A, Tordesillas L, Song X, Reed R, Harkins A, Whiddon S, Nguyen J, Segura C, Zhang C, Yoder S, Sayegh Z, Zhao Y, Messina J, Harro C, Zhang X, Conejo-Garcia J, Berglund A, Sokol L, Zhang J, Rodriguez P, Mule J, Futreal A, Tsai K, Chen P | 2022 | Cancer Discovery | Oncology | Cytonuclear, Highplex FL, TMA | HALO |
| Tumor immune cell clustering and its association with survival in African American women with ovarian cancer | Wilson C, Soupir A, Thapa R, Creed J, Nguyen J, Segura C, Gerke T, Schildkraut J, Peres L, Fridley B | 2022 | PLOS Computational Biology | Cytonuclear, Highplex FL | HALO | |
| Immunologic Gene Signature Analysis Correlates Myeloid Cells and M2 Macrophages with Time to Trabectedin Failure in Sarcoma Patients | Schroder B, Zhang Y, Smythe K, Desai P, Thomas A, Viveiros P, Alexiev B, Obeidin F, Chen E, Cranmer L, Wagner M, Jones R, Campbell J, Pierce R, He Q, Pollack S | 2022 | Cancers | Oncology | Cytonuclear, Highplex FL | HALO |
| Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 Expression | Carossino M, Kenney D, O'Connell A, Montarnaro P, Tseng A, Gertje H, Grosz K, Ericsson M, Huber B, Kurnick S, Subramaniam S, Kirkland T, Walker J, Francis K, Klose A, Paragas N, Bosmann M, Saeed M, Balasuriya U, Douam F, Crossland N | 2022 | Viruses | Infectious Disease | Classifier, Area Quantification, Highplex FL | HALO |
| Multi-modal molecular imaging maps the correlation between tumor microenvironments and nanomedicine distribution | Strittmatter N, Moss J, Race A, Sutton D, Canales J, Ling S, Wong E, Wilson J, Smith A, Howes C, Bunch J, Barry S, Goodwin R, Ashford M | 2022 | Theranostics | Immuno-oncology | Highplex FL | HALO, HALO AI |
| An anti-PD-1ñGITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy | Chan S, Belmar N, Ho S, Rogers B, Stickler M, Graham M, Lee E, Tran N, Zhang D, Gupta P, Sho M, MacDonough T, Woolley A, Kim H, Zhang H, Liu W, Zheng P, Dezso Z, Halliwill K, Ceccarelli M, Rhodes S, Thakur A, Forsyth C, Xiong M, Tan S, Iyer R, Lake M, Digiammarino E, Zhou L, Bigelow L, Longenecker K, Judge R, Liu C, Trumble M, Remis J, Fox M, Cairns B, Akamatsu Y, Hollenbaugh D, Farding F, Alvarez H | 2022 | Nature Cancer | Immuno-oncology | Area Quantification, Cytonuclear, Highplex FL | HALO |
| ZEB1 transcription factor promotes immune escape in melanoma | Plaschka M, Benboubker V, Grimont M, Berthet J, Tonon L, Lopez J, Le-Bouar M, Balme B, Tondeau G, de la Fouchardiere A, Larue L, Puisieux A, Grinberg-Bleyer Y, Bendriss-Vermare N, Dubois B, Caux C, Dalle S, Caramel J | 2022 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Cytonuclear, Multiplex IHC, Highplex FL | HALO |
| Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion | Veprik A, Denwood G, Liu D, Bakar R, Morfin V, McHugh K, Tebeka N, Vetterli L, Yonova-Doing E, Gribble F, Reimann F, Hoehn K, Hemsley P, Ahnfelt-Ronne J, Rorsman P, Zhang Q, Wet H, Cantley J | 2022 | Communications Biology | Metabolism | Cytonuclear, Highplex FL | HALO |
| Extracellular matrix proteins regulate NK cell function in peripheral tissues | Bunting M, Vyas M, Requesens M, Langenbucher A, Schiferle E, Manguso R, Lawrence M, Demehri S | 2022 | Science Advances | Dermatology | Cytonuclear, Highplex FL | HALO |
| Multiplex Immunohistochemical Phenotyping of T Cells in Primary Prostate Cancer | Ozbek B, Ertunc O, Erickson A, Vidal I, Gomes-Alexandre C, Guner G, Hicks J, Jones T, Taube J, Sfanos K, Yegnasubramanian S, DeMarzo A | 2022 | The Prostate | Immuno-oncology | Classifier, Cytonuclear, Highplex FL, Serial Section, Registration, Figure Maker, TMA | HALO |
| Adrenal tropism of SARS-CoV-2 and adrenal findings in a post-mortem case series of patients with severe fatal COVID-19 | Paul T, Ledderose S, Bartsch H, Sun N, Soliman S, Markl B, Ruf V, Herms J, Stern M, Keppler O, Delbridge C, Muller S, Piontek G, Kimoto Y, Schreiber F, Williams T, Neumann J, Knosel T, Schulz H, Spallek R, Graw M, Kirchner T, Walch A, Rudelius M | 2022 | Nature Communications | Infectious Disease | Cytonuclear, Highplex FL | HALO |
| Spatially mapping the immune landscape of melanoma using imaging mass cytometry | Moldoveanu D, Ramsay L, Lajoie M, Anderson-Trocme L, Lingrand M, Berry D, Perus L, Wei Y, Moraes C, Alkallas R, Rajkumar S, Zuo D, Dankner M, Xu E, Bertos N, Najafabadi H, Gravel S, Costantino S, Richer M, Lund A, Del Rincon S, Spatz A, Miller W, Jamal R, Lapointe R, Mes-Masson A, Turcotte S, Petrecca K, Dumitra S, Meguerditchian A, Richardson K, Tremblay F, Wang B, Chergui M, Guiot M, Watters K, Stagg J, Quail D, Mihalcioiu C, Meterissian S, Watson I | 2022 | Science Immunology | Oncology | Highplex FL | HALO |
| Improved cognitive impairments by silencing DMP1 via enhancing the proliferation of neural progenitor cell in Alzheimer-like mice | Zhao H, Wei J, Du Y, Chen P, Liu X, Liu H | 2022 | Aging Cell | Neuroscience | Cytonuclear, Highplex FL | HALO |
| Peritumoral TIGIT+CD20+†B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer | Liu H, Wu J, Xu X, Wang H, Zhang C, Yin S, He Y | 2022 | International Immunopharmacology | Oncology | Cytonuclear, Spatial Analysis, Highplex FL | HALO |
| Subpopulations of cancer-associated fibroblasts link the prognosis and metabolic features of pancreatic ductal adenocarcinoma | Hu B, Wu C, Mao H, Gu H, Dong H, Yan J, Qi Z, Yuan L, Dong Q, Long J | 2022 | Annals of Translational Medicine | Oncology | Cytonuclear, Highplex FL | HALO |
| Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection | Kenney D, O'Connell A, Turcinovic J, Montanaro P, Hekman R, Tamura T, Berneshawi A, Cafiero T, Abdullatif S, Blum B, Goldstein S, Heller B, Gertje H, Bullitt E, Trachtenberg A, Chavez E, Nono E, Morrison C, Tseng A, Sheikh A, Kurnick S, Grosz K, Bosmann M, Ericsson M, Huber B, Saeed M, Balazs A, Francis K, Klose A, Paragas N, Campbell J, Connor J, Emili A, Crossland N, Ploss A, Douam F | 2022 | Cell Reports | Infectious Disease | Area Quantification, Highplex FL | HALO |
| SPOP promotes cervical cancer progress by inducing PD-1 move away from PD-L1 in spatial localization | Wu J, Wu Y, Guo Q, Chen S, Wang S, Zhu J, Wu X, Ju X | 2022 | Research Square | Oncology | Cytonuclear, Spatial Analysis, Highplex FL, TMA | HALO |
| Pilot Trial of Arginine Deprivation Plus Nivolumab and Ipilimumab in Patients with Metastatic Uveal Melanoma | Kraehenbuehl L, Holland A, Armstrong E, O'Shea S, Mangarin L, Chekalil S, Johnston A, Bomalaski J, Erinjeri J, Barker C, Francis J, Wolchok J, Merghoub T, Shoushtari A | 2022 | Cancers | Oncology | Cytonuclear, Highplex FL | HALO |
| Nitric oxide favours tumour-promoting inflammation through mitochondria-dependent and -independent actions on macrophages | Drehmer D, Luiz J, Hernandez C, Alves-Filho J, Hussell T, Townsend P, Moncada S | 2022 | Redox Biology | Oncology | Highplex FL | HALO |
| CD4+†T helper 2 cells suppress breast cancer by inducing terminal differentiation | Boieri M, Malishkevich A, Guennoun R, Marchese E, Kroon S, Trerice K, Awad M, Park J, Iyer S, Kreuzer J, Haas W, Rivera M, Demehri S | 2022 | Journal of Experimental Medicine | Immuno-oncology | Cytonuclear, Highplex FL | HALO |
| Higher proportions of CD39+ tumorresident cytotoxic T cells predict recurrence-free survival in patients with stage III melanoma treated with adjuvant immunotherapy | Attrill G, Owen C, Ahmed T, Vergara I, Colebatch A, Conway J, Nahar K, Thompson J, da Silva I, Carlino M, Menzies A, Lo S, Palendira U, Scolyer R, Long G, Wilmott J | 2022 | Journal of Immuno Therapy of Cancer | Immuno-oncology | Cytonuclear, Spatial Analysis, Highplex FL | HALO |
| Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational study | Shui I, Liu X, Zhao Q, Kim S, Sun Y, Yearley J, Choudhury T, Webber A, Krepler C, Cristescu R, Lee J | 2022 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Cytonuclear, Highplex FL | HALO |
| Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species | Fears A, Beddingfield B, Chirichella N, Slisarenko N, Kileen S, Redmann R, Goff K, Spencer S, Picou B, Golden N, Midkiff C, Bush D, Branco L, Boisen M, Gao H, Montefiori D, Blair R, Doyle-Meyers L, Russel-Lodrigue K, Maness N, Roy C | 2022 | PLOS Pathogens | Infectious Disease | Highplex FL | HALO |
| gp96 Expression in Gliomas and Its Association with Tumor Malignancy and T Cell Infiltrating Level | Li C, Wang Y, Long L, Zhang P, Zhang Y, Ji N | 2022 | Hindawi Journal of Oncology | Immuno-oncology | Spatial Analysis, Highplex FL, Figure Maker | HALO |
| Multiplexed immunofluorescence analysis of CAFmarkers, EZH2 and FOXM1 in Gastric Tissue: Associations with Clinicopathological Parameters and Clinical Outcomes | Sun H, Wang X, Wang X, Tan C, Weng W, Zhang M, Ni S, Wang L, Huang D, Sheng W, Xu M | 2022 | Research Square | Oncology | Cytonuclear, Highplex FL | HALO |
| Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix | Kay E, Paterson K, Riero-Domingo C, Sumpton D, Dabritz J, Tardito S, Boldrini C, Hernandez-Fernaud J, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson L, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson R, Park M, Kirschner K, Miller C, Kamphorst J, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S | 2022 | Nature Metabolism | Oncology, Metabolism | Cytonuclear, Multiplex IHC, Highplex FL | HALO |
| Integrated single-cell analyses decode the developmental landscape of the human fetal spine | Yu H, Tang D, Wu H, Li C, Lu Y, He F, Zhang X, Yang Y, Shi W, Hu W, Zeng Z, Dai W, Ou M, Dai Y | 2022 | iScience | Other | Highplex FL | HALO |
| Establishing a multiplex imaging panel to study T cell development in the thymus in mouse | Allam A, Russell S | 2022 | STAR Protocols | Immunology | Highplex FL | HALO |
| Developing T cells form an immunological synapse for passage through the ?-selection checkpoint | Allam A, Charnley M, Pham K, Russel S | 2022 | Journal of Cell Biology | Immunology | Highplex FL | HALO |
| Endothelial cell death after ionizing radiation does not impair vascular structure in mouse tumor models | Kaeppler J, Chen J, Buono M, Vermeer J, Kannan P, Cheng W, Voukantsis D, Thompson J, Hill M, Allen D, Gomes A, Kersemans V, Kinchesh P, Smart S, Buffa F, Nerlov C, Muschel R, Markelc B | 2022 | EMBO Reports | Oncology | Spatial Analysis, Highplex FL | HALO |
| Non-canonical EphA2 activation underpins PTEN-mediated metastatic migration and poor clinical outcome in prostate cancer | Sachdeva A, Hart C, Kim K, Tawadros T, Oliveira P, Shanks J, Brown M, Clarke N | 2022 | British Journal of Cancer | Oncology | Highplex FL | HALO |
| Approaches to spatially resolving the tumour immune microenvironment of hepatocellular carcinoma | Marsh-Wakefield F, Ferguson A, Liu K, Santhakumar C, McCaughan G, Palendira U | 2022 | Therapeutic Advances in Medical Oncology | Review | Multiplex IHC, Spatial Analysis, Highplex FL | HALO |
| Tau Pathology in Chronic Traumatic Encephalopathy is Primarily Neuronal | Butler M, Dixon E, Stein T, Alvarez V, Huber B, Buckland M, McKee A, Cherry J | 2022 | Journal of Neuropathology & Experimental Neurology | Neuroscience | Multiplex IHC, Spatial Analysis, Highplex FL | HALO, HALO AI |
| Dynamic and adaptive cancer stem cell population admixture in colorectal neoplasia | Vasquez E, Nasreddin N, Valbuena G, Mulholland E, Belnoue-Davis H, Eggington H, Schenck R, Wouters V, Wirapati P, Gilroy K, Lannagan R, Flanagan D, Nujumudeen A, Omwenga S, McCorry A, Easton A, Koelzer V, East J, Morton D, Trusolino L, Maughan T, Campbell A, Loughrey M, Dunne P, Tsantoulis P, Juels D, Tejpar S, Sansom O, Leedham S | 2022 | Cell Stem Cell | Oncology | Highplex FL, Registration | HALO |
| Knockdown of ZBTB11 impedes R-loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancer | Chen L, Liu Z, Tang H, Zhou Z, Chen J, Ma Z, Deng M, Li X, Wu Y, Zheng L, Zhou L, Zheng X, Liu Z | 2022 | Cell Proliferation | Oncology | Multiplex IHC, Highplex FL, Figure Maker | HALO |
| LncRNA scaRNA2 bridges DNA end-resection to homologous recombination repair mediated chemoradioresistance | Yang Y, Chen Y, Shen H, Liu T, Cao K, Wan Z, Du Z, Wang H, Yu Y, Ma S, Li B, Zhang W, Cai J, Gao F | 2022 | Research Square | Oncology | Highplex FL, TMA | HALO |
| Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers | Xu Y, Wei Z, Feng M, Zhu D, Mei S, Wu Z, Feng Q, Chang W, Ji M, Liu C, Zhu Y, Shen L, Yang F, Chen Y, Feng Y, Xu J, Zhu D | 2022 | Cell Reports | Oncology, Immuno-oncology | Multiplex IHC, Highplex FL | HALO |
| Transcriptional and functional analyses of neoantigen-specific CD4 T cells during a profound response to anti-PD-L1 in metastatic Merkel cell carcinoma | Church C, Pulliam T, Longino N, Park S, Smythe K, Makarov V, Riaz N, Jing L, Amezquita R, Campbell J, Gottardo R, Pierce R, Choi J, Chan T, Koelle D, Nghiem P | 2022 | Journal for ImmunoTherapy of Cancer | Oncology, Immuno-oncology | Highplex FL | HALO |
| Modelling aggressive prostate cancers of young men in immune-competent mice, driven by isogenic†Trp53†alterations and†Pten†loss | Mejia-Hernandez J, Keam S, Saleh R, Muntz F, Fox S, Byrne D, Kogan A, Pang L, Huynh J, Litchfield C, Caramia F, Lozano G, He H, You J, Sandhu S, Williams S, Haupt Y, Haupt S | 2022 | Cell Death & Disease | Oncology | Multiplex IHC, Highplex FL, Figure Maker | HALO |
| In vivo tumor immune microenvironment phenotypes correlate with inflammation and vasculature to predict immunotherapy response | Sahu A, Kose K, Kraehenbuehl L, Byers C, Holland A, Tembo T, Santella A, Alfonso A, Li M, Cordova M, Gill M, Fox C, Gonzalez S, Kumar P, Wang A, Kurtansky N, Chandrani P, Yin S, Mehta P, Navarrete-Dechent C, Peterson G, King K, Dusza S, Yang N, Liu S, Phillips W, Guitera P, Rossi A, Halpern A, Deng L, Pulitzer M, Marghoob A, Chen C, Merghoub T, Rajadhyaksha M | 2022 | Nature Communications | Immuno-oncology | Highplex FL, Figure Maker | HALO |
| In†vivo†G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-? secretion by natural killer cells | Zhao X, Peng T, Cao X, Hou Y, Li R, Han T, Fan Z, Zhao M, Chang Y, Chen H, Li C, Huang X | 2022 | Cell Reports | Immunology | Highplex FL | HALO |
| Phase I/II clinical trial of a helper peptide vaccine plus PD-1 blockade in PD-1 antibody-naÔve and PD-1 antibody-experienced patients with melanoma (MEL64) | Vavolizza R, Petroni G, Mauldin I, Chianese-Bullock K, Olson W, Smith K, Dengel L, Haden K, Grosh W, Kaur V, Varhegyi N, Gaughan E, Slingluff C | 2022 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Highplex FL | HALO |
| Unveiling the tumor immune microenvironment of organ-specific melanoma metastatic sites | Conway J, Rawson R, Lo S, Ahmed T, Veregara I, Gide T, Attrill G, Carlino M, Saw R, Thompson J, Spillane A, Shannon K, Shivalingam B, Menzies A, Wilmott J, Long G, Scolyer R, da Silva I | 2022 | Journal for ImmunoTherapy of Cancer | Oncology | Classifier, Spatial Analysis, Highplex FL | HALO |
| Intratumoral administration of CD1c (BDCA-1)+†and CD141 (BDCA-3)+†myeloid dendritic cells in combination with talimogene laherparepvec in immune checkpoint blockade refractory advanced melanoma patients: a phase I clinical trial | Schwarze J, Tijtgat J, Awada G, Cras L, Vasaturo A, Bagnall C, Forsyth R, Dufait I, Tuyaerts S, Riet I, Neyns B | 2022 | Journal for ImmunoTherapy of Cancer | Oncology, Immuno-oncology | Highplex FL | HALO |
| TIE2-high cervical cancer cells promote tumor angiogenesis by upregulating TIE2 and VEGFR2 in endothelial cells | Tan S, Chen Y, Du S, Li W, Liu P, Zhao J, Yang P, Cai J, Gao R, Wang Z | 2022 | Translational Oncology | Oncology | Area Quantification, Highplex FL | HALO |
| Proliferative tumor infiltrating lymphocytes abundance within microenvironment impact clinical outcome in cutaneous B-cell lymphomas | Menguy S, Prochazkova-Carolotti M, Azzi-Martin L, Ferte T, Bresson-Bepoldin L, Rey C, Vergier B, Merlio J, Beylot-Barry M, Pham-Ledard A | 2022 | Journal of Investigative Dermatology | Immuno-oncology | Classifier, Highplex FL | HALO |
| USP14 promotes tryptophan metabolism and immune suppression by stabilizing IDO1 in colorectal cancer | Shi D, Wu X, Jian Y, Wang J, Huang C, Mo S, Li Y, Li F, Zhang C, Zhang D, Zhang H, Huang H, Chen X, Wang Y, Lin C, Liu G, Song L, Liao W | 2022 | Nature Communications | Oncology, Dermatology | Cytonuclear, Highplex FL | HALO, HALO AI |
| Single Cell RNA-Seq Identifies Immune-Related Prognostic Model and Key Signature-SPP1 in Pancreatic Ductal Adenocarcinoma | Chen K, Wang Q, Liu X, Wang F, Ma Y, Zhang S, Shao Z, Yang Y, Tian X | 2022 | Genes | Oncology | Cytonuclear, Highplex FL | HALO |
| Relaxin/insulin-like family peptide receptor 4 (Rxfp4) expressing hypothalamic neurons Q8 modulate food intake and preference in mice | Lewis J, Woodward O, Nuzzaci D, Smith C, Adriaenssens A, Billing L, Brighton C, Phillips B, Tadross J, Kinston S, Ciabatti E, Gottgens B, Tripodi M, Mornigold D, Baker D, Gribble F, Reimann F | 2022 | Molecular Metabolism | Metabolism | ISH/FISH, Highplex FL | HALO |
| Attenuation of relapsing fever neuroborreliosis in mice by IL-17A blockade | Cheng M, Xu J, Ding K, Liu H | 2022 | Proceedings of the National Academy of Sciences | Infectious Disease | Highplex FL | HALO |
| Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system | Weigert A, Zheng X, Nenzel A, Turkowski K, Gunther S, Strack E, Sirait-Fischer E, Elwakeel E, Kur I, Nikam V, Valasarajan C, Winter H, Wissgott A, Voswinkel R, Grimminger F, Brune B, Seeger W, Pullamsetti S, Savai R | 2022 | Nature Communications | Oncology | Spatial Analysis, Highplex FL | HALO |
| Single-cell RNA-seq transcriptomic landscape of human and mouse islets and pathological alterations of diabetes | Chen K, Zhang J, Huang Y, Tian X, Yang Y, Dong A | 2022 | iScience | Metabolism | Cytonuclear, Highplex FL | HALO |
| Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody | Micalizzi D, Che D, Nicholson B, Edd J, Desai N, Lang E, Toner M, Maheswaran S, Ting D, Haber D | 2022 | Proceedings of the National Academy of Sciences | Oncology | Multiplex IHC, Highplex FL | HALO |
| Single-cell RNA sequencing reveals cellular and molecular reprograming landscape of gliomas and lung cancer brain metastases | Sun H, Li L, Lao I, Li X, Xu B, Cao Y, Jin W | 2022 | Clinical and Translational Medicine | Oncology | Cytonuclear, Highplex FL | HALO |
| Impacts of neoadjuvant chemoradiotherapy on the immune landscape of esophageal squamous cell carcinoma | Wen J, Fang S, Hu Y, Xi M, Weng Z, Pan C, Luo K, Ling Y, Lai R, Xie X, Lin X, Lin T, Chen J, Liu Q, Fu J, Yang H | 2022 | EBioMedicine | Immuno-oncology | Cytonuclear, Highplex FL | HALO |
| Method To Visualize the Intratumor Distribution and Impact of Gemcitabine in Pancreatic Ductal Adenocarcinoma by Multimodal Imaging | Strittmatter N, Richards F, Race A, Ling S, Sutton D, Nilsson A, Wallez Y, Barnes J, Maglennon G, Gopinathan A, Brais R, Wong E, Serra M, Atkinson J, Smith A, Wilson J, Hamm G, Johnson T, Dunlop C, Kaistha B, Bunch J, Sansom O, Takats Z, Andren P, Lau A, Barry S, Goodwin R, Jodrell D | 2022 | Analytical Chemistry | Oncology | Classifier, Highplex FL | HALO, HALO AI |
| Xiaoyaosan ethyl acetate fraction alleviates depression-like behaviors in CUMS mice by promoting hippocampal neurogenesis via modulating the IGF-1R_/PI3K/Akt signaling pathway | Zeng J, Ji Y, Luan F, Hu J, Rui Y, Liu Y, Rao Z, Liu R, Zeng N | 2022 | Journal of Experimental & Clinical Cancer Research | Neuroscience | Highplex FL | HALO |
| SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer | Li, T, Jin K, Li H, Ye L, Li P, Jiang B, Lin X, Liao Z, Zhang H, Shi S, Lin M, Fei Q, Xiao Z, Xu H, Liu L, Yu X, Wu W | 2022 | Cancer Letters | Immuno-oncology | Highplex FL, TMA | HALO |
| Presence of Tim3+ and PD-1+ CD8+ T cells identifies microsatellite stable colorectal carcinomas with immune exhaustion and distinct clinicopathological features | Klapholz M, Drage M, Srivastava A, Anderson A | 2022 | The Journal of Pathology | Oncology | Multiplex IHC, Highplex FL | HALO |
| Impact of HPV status on immune responses in head and neck squamous cell carcinoma | Qureshi H, Zhu X, Yang G, Steadele M, Pierce R, Futran N, Lee S, Mendez E, Houghton A | 2022 | Oral Oncology | Oncology | Multiplex IHC, Spatial Analysis, Highplex FL | HALO |
| Developing a Nomogram for Preoperative Prediction of Cervical Cancer Lymph Node Metastasis by Multiplex Immunofluorescence | Wu J, Guo Q, Zhu J, Wu Y, Liang S Chen S, Wang S, Ju X, Wu X | 2022 | Authorea | Oncology | Spatial Analysis, Highplex FL, TMA | HALO |
| PERK reprograms hematopoietic progenitor cells to direct tumor-promoting myelopoiesis in the spleen | Liu M, Wu C, Luo S, Hua Q, Chen H, Weng Y, Xu J, Lin H, Wang L, Li J, Zhu L, Guo Z, Zhuang S, Kang T, Zheng L | 2022 | Journal of Experimental Medicine | Oncology | Highplex FL, Registration | HALO |
| Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer | Ramos R, Missolo-Koussou Y, Gerber-Ferder Y, Bromley C, Bugatti M, Nunez N, Boari J, Richer W, Menger L, Denizeau J, Sedlik C, Caudana P, Kotsias F, Niborski L, Viel S, Bohec M, Lameiras S, Baulande S, Lesage L, Nicolas A, Meseure D, Vincent-Salomon A, Reyal F, Dutertre C, Ginhoux F, Vimeux L, Donnadieu E, Buttard B, Galon J, Zelenay S, Vermi W, Guermonprez P, Piaggio E, Helft J | 2022 | Cell | Immuno-oncology | Classifier, Spatial Analysis, Highplex FL | HALO |
| A Phase 1/2 Trial Combining Avelumab and Trabectedin for Advanced Liposarcoma and Leiomyosarcoma | Wagner M, Zhang Y, Cranmer L, Loggers E, Black G, McDonnell S, Maxwell S, Johnson R, Moore R, Hermida de Viveriros P, Aicher L, Smythe K, He Q, Jones R, Pollack S | 2022 | Clinical Cancer Research | Oncology | Highplex FL | HALO |
| Anatomic and functional mapping of human uterine innervation | Pinsard M, Mouchet N, Dion L, Bessede T, Bertrand M, Darai E, Bellaud P, Loget P, Mazaud-Guittot S, Morandi X, Leveque J, Lavoue V, Duraes M, Timoh K | 2022 | Fertility and Sterility | Other | Highplex FL | HALO |
| Neoantigen-specific CD4+ T cells in human melanoma have diverse differentiation states and correlate with CD8+ T cell, macrophage, and B cell function | Veatch J, Lee S, Shasha C, Singhi N, Szeto J, Moshiri A, Kim T, Smythe K, Kong P, Fitzgibbon M, Jesernig B, Bhatia S, Tykodi S, Hall E, Byrd D, Thompson J, Pillarisetty V, Duhen T, Houghton A, Newell E, Gottardo R, Riddell S | 2022 | Cancer Cell | Immuno-oncology | Spatial Analysis, Highplex FL | HALO |
| A molecular analysis of neural olfactory placode differentiation in human pluripotent stem cells | Bricker R, Bhaskar U, Titone R, Carless M, Barberi T | 2022 | Stem Cells and Development | Other | Highplex FL, Figure Maker | HALO |
| PHGDH heterogeneity potentiates cancer cell dissemination and metastasis | Rossi M, Altea-Manzano P, Demicco M, Doglioni G, Bornes L, Fukano M, Vandekeere A, Cuadros A, Fernandez-Garcia J, Riera-Domingo C, Jauset C, Planque M, Alkan H, Nittner D, Zuo D, Broadfield L, Parik S, Pane A, Rizzollo F, Rinaldi G, Zhang T, Teoh S, Aurora A, Karras P, Vermeire I, Broekaert D, Van Elsen J, Knott M, Orth M, Demeyer S, Eelen G, Dobrolecki L, Bassez A BRussel T, Sotlar K, Lewis M, Bartsch H, Wuhrer M, van Veelen P, Carmeliet J, Cools J, Morrison S, Marine J, Lambrechts D, Mazzone M, Hannon G, Lunt S, Grunewald T, Park M, van Rheenen J, Fendt S | 2022 | Nature | Oncology | Highplex FL | HALO |
| Potentiating adoptive cell therapy using synthetic IL-9 receptors | Kalbasi A, Siurala M, Su L, Tariveranmoshabad M, Picton L, Ravikumar P, Li P, Lin J, Escuin-Ordinas H, Da T, Kremer S, Sun A, Castelli S, Agarwal S, Scholler J, Song D, Rommel P, Radaelli E, Young R, Leonard W, Ribas A, June C, Gargia K | 2022 | Nature | Immuno-oncology | Highplex FL | HALO |
| Tumor cells modulate macrophage phenotype in a novel in vitro co-culture model of the non-small cell lung cancer tumor microenvironment | Park J, Chandra R, Cai L, Ganguly D, Li H, Toombs J, Girard L, Brekken R, Minna J | 2022 | Journal of Thoracic Oncology | Immuno-oncology | Highplex FL | HALO |
| A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma | Rappold P, Vuong L, Leibold J, Chakiryan N, Curry M, Kuo F, Sabio E, Jiang H, Nixon B, Liu M, Berglund A, Silagy A, Mascareno A, Golkaram M, Marker M, Reising A, Savchenko A, Milholland J, Chen Y, Russo P, Coleman J, Reznik E, Manley B, Ostrovnaya I, Makarav V, DiNatale R, Blum K, Ma X, Chowell D, Li M, Solit D, Lowe S, Chan T, Motzer R, Voss M, Hakimi A | 2022 | Cancer Discovery | Oncology | Highplex FL | HALO |
| Spatiotemporal expression pattern of Progesterone Receptor Component (PGRMC) 1 in endometrium from patients with or without endometriosis or adenomyosis | Thieffry C, Van Wynendaele M, Samain L, Tyteca D, Pierreux C, Marbaix E, Henriet P | 2022 | The Journal of Steroid Biochemistry and Molecular Biology | Other | ISH/FISH, Highplex FL | HALO, HALO AI |
| Neoadjuvant Therapy Induces a Potent Immune Response to Sarcoma, Dominated by Myeloid and B Cells | Goff P, Riolobos L, LaFleur B, Spraker M, Seo Y, Smythe K, Campbell J, Pierce R, Zhang Y, He Q, Kim E, Schaub S, Kane G, Mantilla J, Chen E, Ricciotti R, Thompson M, Cranmer L, Wagner M, Loggers E, Jones R, Murphy E, Blumenschein W, McClanahan T, Earls J, Flanagan K, LaFranzo N, Kim T, Pollack S | 2022 | Clinical Cancer Research | Immuno-oncology | Highplex FL | HALO |
| Targeted RNA editing in brainstem alleviates respiratory dysfunction in a mouse model of Rett syndrome | Sinnamon J, Jacobson M, Yung J, Mandel G | 2022 | Proceedings of the National Academy of Sciences | Neuroscience | Highplex FL | HALO |
| Characterization of anti-CD79b/CD3 bispecific antibody, a potential therapy for B cell malignancies | Wang J, Li C, He K, Kuang Z, Lu J, Yao Y, He F, Li N, Li L, Fu F, Wu Z, Zhou S, Kang D, Qiu X, Wu M, Liu Y, Cao X, Xu M, Chen B, Wu W, Guo F | 2022 | Cancer Immunology, Immunotherapy | Immuno-oncology | Highplex FL | HALO |
| Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis | Sabate-Perez A, Romero M, Sanchez-Fernandez-de-Landa P, Carobbio S, Mouratidis M, Sala D, Engel P, Villena J, Virtue S, Vidal-Puig A, Palacin M, Testar X, Zorzano A | 2022 | Autophagy | Metabolism | Highplex FL | HALO, HALO AI |
| Epigallocatechin-3-gallate reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis | Li H, Qiao C, Zhao L, Jing Q, Xue D, Zhang Y | 2022 | Journal of Leukocyte Biology | Immunology | Highplex FL | HALO |
| Notch signaling promotes mature T-cell lymphomagenesis | Gao X, Wang C, Abdelrahman S, Kady N, Murga-Zamalloa C, Gann P, Sverdlov M, Wolfe A, Polk A, Brown N, Bailey N, Inamdar K, Casavilca-Zambrano S, Montes J, Barrionuevo C, Taxa L, Reneau J, Siebel C, Maillard I, Wilcox R | 2022 | Cancer Research | Immuno-oncology | Highplex FL | HALO, HALO AI |
| Plasma CD27, a surrogate of the intratumoral CD27-CD70 interaction, correlates with immunotherapy resistance in renal cell carcinoma | Benhamouda N, Sam I, Epaillard N, Gey A, Phan L, Pham H, Gruel N, Saldmann A, Pineau J, Hasan M, Quiniou V, Nevoret C, Verkarre V, Libri V, Mella S, Granier C, Broudin C, Ravel P, De Guillebon E, Mauge L, Helley D, Jabla B, Chaput N, Albiges L, Katsahian S, Adam J, Mejean A, Adotevi O, Vano Y, Oudard S, Tartour E | 2022 | Clinical Cancer Research | Oncology | Spatial Analysis, Highplex FL | HALO |
| Ablation of the endoplasmic reticulum stress kinase PERK induces paraptosis and type I interferon to promote anti-tumor T cell responses | Mandula J, Chang S, Mohamed E, Jimenez R, Sierra-Mondragon R, Chang D, Obermayer A, Moran-Segura C, Das S, Vazquez-Martinez J, Prieto K, Chen A, Smalley K, Czerniecki B, Forsyth P, Koya R, Ruffell B, Cubillos-Ruiz J, Munn D, Shaw T, Conejo-Garcia J, Rodriguez P | 2022 | Cancer Cell | Immuno-oncology | Highplex FL | HALO |
| CD103 and periplakin are potential biomarkers for response of metastatic melanoma to pembrolizumab | Edmonds N, Flores S, Mahmutovic A, Young S, Mauldin I, Slingluff C | 2022 | Melanoma Research | Immuno-oncology | Highplex FL | HALO |
| High endothelial venules associated with better prognosis in esophageal squamous cell carcinoma | Li H, Tang L, Han X, Zhong L, Gao W, Chen Y, Huang J, Wen Z | 2022 | Annals of Diagnostic Pathology | Oncology | Object Colocalization, Spatial Analysis, Highplex FL | HALO |
| Evolutionary trajectory of receptor binding specificity and promiscuity of the spike protein of SARS-CoV-2 | Planchais C, Reyes-Ruiz A, Lacombe R, Zarantonello A, Lecerf M, Revel M, Roumenina L, Atanasov B, Mouquet H, Dimitrov J | 2022 | Protein Science | Infectious Disease | Area Quantification, Highplex FL | HALO |
| Novel post-acquisition image processing to attenuate red blood cell autofluorescence for quantitative image analysis | Bouffard N, Lee K, DeLance N, Clason T, Chatterjee N, Taatjes D | 2022 | Histochemistry and Cell Biology | Other | Highplex FL | HALO |
| Angiogenesis modulation-mediated inhibitory effects of tacrolimus on hypertrophic scar formation | Shen Y, Jin R, Liang X, Deng Z, He J, Ding Y, Ding F, Lu L, Liu F, Yang J | 2022 | Microvascular Research | Dermatology | Highplex FL | HALO |
| Chronic rhinosinusitis patients display an aberrant immune cell localisation with enhanced S. aureus biofilm metabolic activity and biomass | Shaghayegh G, Cooksley C, Bouras G, Panchatcharam B, Idrizi R, Jana M, Ellis S, Psaltis A, Wormald P, Vreugde S | 2022 | Journal of Allergy and Clinical Immunology | Immunology, Infectious Disease | Classifier, Spatial Analysis, Highplex FL | HALO |
| Low CD8 T Cell Counts Predict Benefit from Hypoxia-Modifying Therapy in Muscle-Invasive Bladder Cancer | Smith V, Mukherjee D, Tsakiroglou A, Baker A, Mistry H, Choudhury A, Hoskin P, Illidge T, West C | 2022 | Cancers | Immuno-oncology | Highplex FL, TMA | HALO |
| NUC-7738 regulates _-catenin signalling resulting in reduced proliferation and self-renewal of AML cells | Shahid A, Um I, Elshani M, Zhang Y, Harrison D | 2022 | PLOS One | Oncology | Highplex FL | HALO, HALO AI |
| Innate immune receptor C5aR1 regulates cancer cell fate and can be targeted to improve radiotherapy in tumours with immunosuppressive microenvironments | Beach C, MacLean D, Majorova D, Melemenidis S, Nambiar D, Kim R, Valbuena G, Guglietta S, Krieg C, Darvish D, Suwa T, Eason A, Domingo E, Moon E, Jiang D, Jiang Y, Koong A, Woodruff T, Graves E, Maughan T, Buczacki S, Stucki M, Le Q-T, Leedham S, Giaccia A, Olcina M | 2023 | bioRxiv | Immuno-oncology | Classifier, Area Quantification, Highplex FL | HALO |
| Single-cell RNA sequencing identifies a population of human liver-type ILC1s | Kramer B, Nalin A, Ma F, Eickoff S, Lutz P, Leonardelli S, Foeser F, Finnemann C, Hack G, Raabe J, ToVinh M, Ahmad S, Hoffmeister C, Kaiser K, Manekeller S, Branchi V, Bald T, Holzel M, Huneburg R, Nischalke H, Semaan A, Langhans B, Kaczmarek D, Benner B, Lordo M, Kowalski J, Gerhardt A, Timm J, Toma M, Mohr R, Turler A, Charpentier A, van Bremen T, Felmann G, Sattler A, Kotsch K, Abdallah A, Strassburg C, Spengler U, Carson W, Mundy-Bosse B, Pellegrini M, O'Sullivan T, Greud A, Nattermann J | 2023 | Cell Reports | Immunology | Highplex FL | HALO |
| Tertiary lymphoid structures in desmoplastic melanoma have increased lymphocyte density, lymphocyte proliferation, and immune cross talk with tumor when compared to non-desmoplastic melanomas | Edmonds N, Gradecki S, Katyal P, Lynch K, Stowman A, Gru A, Engelhard V, Slingluff Jr. C, Mauldin I | 2023 | OncoImmunology | Immuno-oncology | Highplex FL | HALO |
| Autologous T cell therapy for MAGE-A4+ solid cancers in HLA-A*02+ patients: a phase 1 trial | Hong D, Van Tine B, Biswas S, McAlpine C, Johnson M, Olszanski A, Clarke J, Araujo D, Blumenschein G, Kebriaei P, Lin Q, Tipping A, Sanderson J, Wang R, Trivedi T, Annareddy T, Bai J, Rafail S, Sun A, Fernandes L, Navenot J-M, Bushman F, Everett J, Karadeniz D, Broad R, Isabelle M, Naidoo R, Bath N, Betts G, Wolchinsky Z, Batrakou D, Van Winkle E, Elefant E, Ghobadi A, Cashen A, Grand'Maison A, McCarthy P, Fracasso P, Norry E, Williams D, Druta M, Liebner D, Odunsi K, Butler M | 2023 | Nature Medicine | Immuno-oncology | ISH/FISH, Spatial Analysis, Highplex FL | HALO |
| A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration | Hwang P, Mathur J, Cao Y, Almeida J, Ye J, Morikis V, Cornish D, Clarke M, Stewart S, Pathak A, Longmore G | 2023 | Developmental Cell | Oncology | Highplex FL, Registration | HALO |
| Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods | Bayer F, Bejarano D, Bartacchi G, Doffin A-C, Gobbini E, Hubert M, Li L, Meiser P, Pedde A-M, Posch W, Rupp L, Schlitzer A, Schmitz M, Schraml B, Uderhardt S, Balladeau-Guilemond J, Wilflingseder D, Zaderer V, Bottcher J | 2023 | European Journal of Immunology | Other | Highplex FL | HALO |
| Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal‚Ñ¢-TSA multiplex immunohistochemistry | Quigley L, Pang L, Tavancheh E, Ernst M, Behren A, Huynh J, Da Gama Duarte J | 2023 | STAR Protocols | Other | Highplex FL | HALO |
| Standardized pathology screening of mature Tertiary Lymphoid Structures in cancers | Vanhersecke L, Bougouin A, Crombe A, Brunet M, Sofeu C, Parrens M, Pierron H, Bonhomme B, Lembege N, Rey C, Velasco V, Soubeyran I, Begueret H, Bessede A, Bellera C, Scoazec J-Y, Italiano A, Fridman C, Fridman W, Le Loarer F | 2023 | Laboratory Investigation | Other | Highplex FL, Registration | HALO |
| Effects of electroacupuncture on the kisspeptin-gonadotropin-releasing hormone (GnRH) /luteinizing hormone (LH) neural circuit abnormalities and androgen receptor expression of kisspeptin/neurokinin B/dynorphin neurons in PCOS rats. | Xu G, Zhao X, Li Z, Hu J, Li X, Li J, Chen Y | 2023 | Journal of Ovarian Research | Other | Highplex FL | HALO |
| Fucosylation of HLA-DRB1 regulates CD4+ T cell-mediated anti-melanoma immunity and enhances immunotherapy efficacy | Lester D, Burton C, Gardner A, Innamarato P, Kodumudi K, Liu Q, Adhikari E, Ming Q, Williamson D, Frederick D, Sharova T, White M, Markowitz J, Cao B, Nguyen J, Johnson J, Beatty M, Mockabee-Macias A, Mercurio M, Watson G, Chen P-L, McCarthy S, MoranSegura C, Messina J, Thomas K, Darville L, Izumi V, Koomen J, Pilon-Thomas S, Ruffell B, Luca V, Haltiwanger R, Wang X, Wargo J, Boland G, Lau E | 2023 | Nature Cancer | Immuno-oncology | Highplex FL, Registration | HALO |
| Inhibition of indoleamine dioxygenase leads to better control of tuberculosis adjunctive to chemotherapy | Singh B, Moodley C, Singh D, Escobedo R, Sharan R, Arora G, Ganatra S, Shivanna V, Gonzalez O, Hall-Ursone S, Dick Jr. E, Kaushal D, Alvarez X, Mehra S | 2023 | JCI Insight | Infectious Disease, Fibrosis | Area Quantification, Highplex FL | HALO |
| Combined KRASG12C and SOS1 inhibition enhances and extends the anti-tumor response in KRASG12C-driven cancers by addressing intrinsic and acquired resistance | Thatikonda V, Lu H, Jurado S, Kostyrko K, Bristow C, Bosch K, Feng N, Gao S, Gerlach D, Gmachi M, Lieb S, Jeschko A, Machado A, Marszalek E, Mahendra M, Jaeger P, Sorokin A, Strauss S, Trapani F, Kopetz S, Vellano C, Petronczki M, Kraut N, Heffernan T, Marszalek J, Pearson M, Waizenegger I, Hofmann M | 2023 | bioRxiv | Oncology | ISH/FISH, Multiplex IHC, Highplex FL, Figure Maker | HALO |
| Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial | Tian J, Chen J, Chao S, Pelka K, Giannakis M, Hess J, Burke K, Jorgji V, Sindurakar P, Braverman J, Mehta A, Oka T, Huang M, Lieb D, Spurrell M, Allen J, Abrams T, Clark J, Enzinger A, Enzinger P, Klempner S, McCleary N, Meyerhardt J, Ryan D, Yurgelun M, Kanter K, Van Seventer E, Baiev I, Chi G, Jarnagin J, Bradford W, Wong E, Michel A, Fetter I, Siravegna G, Gemma A, Sharpe A, Demehri S, Leary R, Campbell C, Yilmaz Om Getz G, Parikh A, Hacohen N, Corcoran R | 2023 | Nature Medicine | Oncology | Highplex FL | HALO |
| Gold Nanoparticles for Monitoring of Mesenchymal Stem Cell–Loaded Bioresorbable Polymeric Wraps for Arteriovenous Fistulas | Barcena A, Perez J, Damasco J, Bernardino M, San Valentin E, Klusman C, Martin B, Cortes A, Canlas G, Heralde III, F, Avritscher R, Fowlkes N, Bouchard R, Cheng J, Huang S, Melancon M | 2023 | bioRxiv | Biomaterials | Highplex FL | HALO |
| Discovery of NRG1-VII: a novel myeloid-derived class of NRG1 isoforms | Berrocal-Rubio M, Pawer Y, Dinevska M, De Paoli-Iseppi R, Widodo S, Rajab N, De Nardo W, Hallab J, Li A, Mantamadiotis T, Clark M, Wells C | 2023 | bioRxiv | Immunology | Highplex FL | HALO |
| Single-cell spatial immune landscapes of primary and metastatic brain tumours | Karimi E, Yu M, Maritan S, Perus L, Rezanejad M, Sorin M, Kankner M, Fallah P, Doré S, Zuo D, Fiset B, Kloosterman D, Ramsay L, Wei Y, Lam S, Alsajjan R, Watson I, Urgoiti G, Park M, Randsma D, Senger D, Chan J, Akkari L, Petrecca K, Guiot M-C, Siegel P, Quail D, Walsh L | 2023 | Nature | Immuno-oncology | Highplex FL | HALO |
| Multiplex immune profiling reveals the role of serum immune proteomics in predicting response to preoperative chemotherapy of gastric cancer | Tang Z, Gu Y, Shi Z, Min L, Zhang Z, Zhou P, Luo R, Wang Ym, Cui Y, Sun Y, Wang X | 2023 | Cell Reports Medicine | Immuno-oncology | Highplex FL | HALO |
| Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses | Hirz T, Mei S, Sarkar H, Kfoury Y, Wu S, Verhoeven B, Subtelny A, Zlatev D, Wszolek M, Salari K, Murray E, Chen F, Macosko E, Wu C, Scadden D, Dahl D, Baryawno N, Saylor P, Kharchenko P, Sykes D | 2023 | Nature Communications | Immuno-oncology | Highplex FL, Figure Maker, HALO AI | HALO |
| Impact of aging on the immunological and microbial landscape of the lung during non-tuberculous mycobacterial infection | Cinco I, Rhoades N, Napier E, Davies M, Allison D, Kohama S, Bermudez L, Winthrop K, Fuss C, Spindel E, Messaoudi I | 2023 | bioRxiv | Infectious Disease | Highplex FL | HALO |
| Effects of KMT2D mutation and its exon 39 mutation on the immune microenvironment and drug sensitivity in colorectal adenocarcinoma | Liu C, Jin Y, Zhang H, Yan J, Guo Y, Bao X, Zhao P | 2023 | Heliyon | Oncology | Highplex FL | HALO |
| NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis | Ilter D, Drapela S, Schild T, Ward N, Adhikari E, Low V, Asara J, Oskarsson T, Lau E, DeNicola G, McReynolds M, Gomes A | 2023 | Redox Biology | Oncology, Metabolism | Classifier, Highplex FL, TMA | HALO |
| Spatial transcriptomics analysis of zone-dependent hepatic ischemia-reperfusion injury murine model | Xin J, Yang T, Wu X, Wu Y, Liu Y, Liu X, Jiang M, Gao W | 2023 | Communications Biology | Gastroenterology | Highplex FL | HALO |
| Coordinated activation of c-Src and FOXM1 drives tumor cell proliferation and breast cancer progression | Nandi I, Smith H, Sanguin-Gendreau V, Ji L, Pacis A, Papavasiliou V, Zuo D, Nam S, Attala S, Kim S, Lusson S, Kuasne H, Fortier A, Savage P, Ramirez C, Park M, Katzenellenbogen J, Katzenellenbogen B, Muller W | 2023 | Journal of Clinical Investigation | Oncology | Highplex FL | HALO |
| Oncogenic KrasG12D specific non-covalent inhibitor reprograms tumor microenvironment to prevent and reverse early pre-neoplastic pancreatic lesions and in combination with immunotherapy regresses advanced PDAC in a CD8+ T cells dependent manner | Mahadevan K, McAndrews K, LeBlue V, Yang S, Lyu H, Li B, Sockwell A, Kirtley M, Morse S, Diaz B, Kim M, Feng N, Lopez A, Guerrero P, Sugimoto H, Arian K, Ying H, Barekatain Y, Kelly P, Maitra A, Heffernan T, Kalluri R | 2023 | bioRxiv | Oncology | FISH, Highplex FL | HALO |
| PD-1+CD8+ T Cells Proximal to PD-L1+CD68+ Macrophages Are Associated with Poor Prognosis in Pancreatic Ductal Adenocarcinoma Patients | Yang X, Wang G, Song Y, Zhuang T, Li Y, Xie Y, Fei X, Zhao Y, Xu D, Hu Y | 2023 | Cancers | Oncology, Immuno-oncology | Classifier, Spatial Analysis, Highplex FL, Registration, TMA | HALO |
| Donor Batf3 inhibits murine lung allograft rejection and airway fibrosis | Watanabe T, Lam C, Oliver J, Oishi H, Teskey G, Beber S, Boonstra K, Mauricio J, Buhari H, Joe B, Guan Z, Horie M, Keshavjee S, Martinu T, Juvet S | 2023 | Mucosal Immunity | Other, Fibrosis | Highplex FL | HALO |
| Loss of Hepatic Leucine-Rich Repeat-Containing G-Protein Coupled Receptors 4 and 5 Promotes Nonalcoholic Fatty Liver Disease | Saponara E, Penno C, Orsini V, Wang Z, Fischer A, Aebi A, Matadamas-Guzman M, Brun V, Fischer B, Brousseau M, O'Donnell P, Turner J, Meyer A, Bollepalli L, d'Ario G, Roma G, Carbone W, Annunziato S, Obrecht M, Beckmann N, Saravanan C, Osmont A, Tropberger P, Richards S, Genoud C, Ley S, Ksiazek I, Nigsch F, Terracciano L, Schadt H, Bouwmeester T, Tchorz J, Ruffner H | 2023 | The American Journal of Pathology | Gastroenterology | Highplex FL | HALO |
| Single-cell analysis reveals changes in BCG vaccine-injected mice modeling tuberculous meningitis brain infection | Zhang X, Zhao Z, Wu Q, Wang L, Li L, Wang M, Ren Y, Pan L, Tang H, Li F | 2023 | Cell Reports | Infectious Disease | Multiplex IHC, Highplex FL | HALO |
| Spatial multi-omics revealed the impact of tumor ecosystem heterogeneity on immunotherapy efficacy in patients with advanced non-small cell lung cancer treated with bispecific antibody | Song X, Xiong A, Wu F, Li X, Wang J, Jiang T, Chen P, Zhang X, Zhao Z, Liu H, Cheng L, Zhao C, Wang Z, Pan C, Cui X, Xu T, Luo H, Zhou C | 2023 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Classifier, Highplex FL | HALO |
| Melanotransferrin Functions as a Pro-Oncogenic WNT Agonist: A Yin-Yang Relationship in Melanoma with the WNT Antagonist and Metastasis Suppressor, NDRG1 | Paluncic J, Cholam M, Lane D, Skoda J, Park K, Chiang S, Bae D, Scolyer R, Afroz R, Babu G, Wilmott J, Loh K, Jansson P, Dharmasiviam M, Huang M, Zhao X, Kovacevic Z, Richardson D | 2023 | bioRxiv | Oncology | Highplex FL | HALO |
| Co-enrichment of CD8-positive T cells and macrophages is associated with clinical benefit of tislelizumab in solid tumors | Ye D, Desai J, Shi J, Liu S, Shen W, Liu T, Shi Y, Wang D, Liang L, Yang S, Ma X, Jin W, Zhang P, Huang R, Shen Z, Zhang Y, Wu Y | 2023 | Biomarker Research | Immuno-oncology | Spatial Analysis, Highplex FL | HALO |
| Single-cell profiling reveals pathogenic role and differentiation trajectory of granzyme K+ CD8+T cells in primary Sjögren’s syndrome | Xu T, Zhu H, Ma J, Li X, Luo P, Li Y, Lian Z, Gao C | 2023 | JCI Insight | Immunology | Highplex FL | HALO |
| SARS-CoV-2 infection induces DNA damage, through CHK1 degradation and impaired 53BP1 recruitment, and cellular senescence | Gioia U, Tavella S, Martinez-Orellana P, Cicio G, Colliva A, Ceccon M, Cabrini M, Henriques A, Fumagalli V, Paldino A, Presot E, Rajasekharan S, Iacomino N, Pisati F, Matti V, Sepe S, Conte M, Barozzi S, Lavagnino Z, Carletti T, Volpe M, Cavalcante P, Iannacone M, Rampazzo C, Bussani R, Tripodo C, Zacchigna S, Marcello A, di Fagagna F | 2023 | Nature Cell Biology | Infectious Disease | ISH/FISH, Highplex FL | HALO |
| TIPE2 Inhibits MGD Inflammation by Regulating Macrophage Polarization | Zhao S, Shen Y, Wu S, Shao Y, Shi R, Yan Y, Zhao H | 2023 | Journal of Personalized Medicine | Immunology | Area Quantification, Highplex FL | HALO |
| Loss of Peroxiredoxin IV Protects Mice from Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Cancer Development | Thapa P, Jiang H, Ding N, Hao Y, Alshahrani A, Lee E, Fujii J, Wei Q | 2023 | Antioxidants | Oncology | Multiplex IHC, Highplex FL | HALO |
| Bi-allelic mutation in SEC16B alters collagen trafficking and increases ER stress | El-Gazzar A, Voraberger B, Rauch F, Mairhofer M, Schmidt K, Guillemyn B, Mitulovic G, Reiterer V, Haun M, Mayr M, Mayr J, Kimeswenger S, Drews O, Saraff V, Shaw N, Fratzl-Zelman N, Symoens S, Farhan H, Hogler W | 2023 | EMBO Molecular Medicine | Other | Area Quantification, Highplex FL | HALO |
| Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node Metastasis | Su X, Brassard A, Bartolomucci A, Dhoparee-Dooma I, Qiu Q, Tsering T, Rohanizadeh R, Koufos O, Giannias B, Bourdeau F, Feng L, Leo S, Sangwan V, Tankel J, Quail D, Spicer J, Burnier J, Bailey S, Ferri L, Cools-Lartigue J | 2023 | bioRxiv | Immuno-oncology | Area Quantification, Highplex FL | HALO |
| Chronic immune activation and gut barrier dysfunction is associated with neuroinflammation in ART-suppressed SIV+ rhesus macaques | Byrnes S, Busman-Sahay K, Angelovich T, Younger S, Taylor-Brill S, Nekorchuk M, Bondoc S, Dannay R, Terry M, Cochrane C, Jenkins T, Roche M, Deleage C, Bosinger S, Paiardini M, Brew B, Estes J, Churchill M | 2023 | PLOS Pathogens | Infectious Disease | Area Quantification, Object Colocalization, ISH, Highplex FL | HALO |
| Development of a deep pathomics score for predicting hepatocellular carcinoma recurrence after liver transplantation | Qu W, Tian M, Lu G, Zhou Y, Liu W, Tang Z, Yao Z, Huang R, Zhu G, Jiang X, Tao C, Fang Y, Gao J, Wu X, Chen J, Zhao Q, Yang R, Chu T, Zhou J, Fan J, Yu J, Shi Y | 2023 | Hepatology International | Oncology | ISH, Spatial Analysis, Highplex FL | HALO |
| Early Identication of High-Risk Patients with HCC Relapse: A Spatial Immune Scoring System Enabled by Articial Intelligence | Sun C, Jia G, He P, Goh D, Li F, Wee F, Sun M, Dai T, Lim J, Hao S, Liu L, Yeong J, Tian Z | 2023 | Research Square | Oncology | Highplex FL | HALO |
| Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer | Shakfa N, Li D, Conseil G, Lightbody E, Wilson-Sanchez J, Hamade A, Chenard S, Jawa N, Laight B, Afriyie-Asante A, Tyryshkin K, Koebel M, and Koti M | 2023 | Journal for ImmunoTherapy of Cancer | Oncology | Highplex FL | HALO, HALO Link |
| Prognostic value of tumor immune microenvironment factors in patients with stage I lung adenocarcinoma | Xue Q, Wang Y, Zheng Q, Chen L, Lin Y, Jin Y, Shen X, Li Y | 2023 | American Journal of Cancer Research | Oncology | Classifier, Multiplex IHC, Highplex FL | HALO |
| Immune Checkpoint Neuropilins as Novel Biomarkers and Therapeutic Targets for Pancreatic Cancer | He L, Zhang X, Lao M, Zhang H, Yang H, Bai X | 2023 | Cancers | Immuno-oncology | Highplex FL | HALO |
| Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection | Premadasa L, Lee E, McDew-White M, Alvarez X, Jayakumar S, Ling B, Okeoma C, Byrareddy S, Kulkarni S, and Mohan M | 2023 | JCI Insight | Infectious Disease | Area Quantification, Highplex FL | HALO |
| Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer | Wang F, Long J, Li L, Wu Z, Da T, Wang X, Huang C, Jiang Y, Yao X, Ma H, Lian Z, Zhao Z, Cao J | 2023 | Science Advances | Oncology | Multiplex IHC, Spatial Analysis, Highplex FL | HALO |
| High tumor mutational burden predicts favorable response to anti-PD-(L)1 therapy in patients with solid tumor: a real-world pan-tumor analysis | Jung J, Heo Y, Park S | 2023 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Spatial Analysis, Highplex FL | HALO |
| Transcriptional-translational conflict is a barrier to cellular transformation and cancer progression | Jana S, Brahma S, Arora S, Wladyka C, Hoang P, Blinka S, Hough R, Horn J, Liu Y, Wang L, Depeille P, Smith E, Montgomery R, Lee J, Haffner M, Vakar-Lopez F, Grivas P, Wright J, Lam H, Black P, Roose J, Ryazanov A, Subramaniam A, Henikoff S, and Hsieh A | 2023 | Cancer Cell | Oncology | Multiplex IHC, Highplex FL | HALO |
| Geospatial characterization of immune cell distributions and dynamics across the microenvironment in clear cell renal cell carcinoma | Chakiryan N, Kim Y, Berglund A, Chang A, Kimmel G, Hajiran A, Nguyen J, Moran-Segura C, Saeed-Vafa D, Katende E, Lopez-Blanco N, Chahoud J, Rappold P, Spiess P, Fournier M, Jeong D, Wang L, Teer J, Dhillon J, Kuo F, Hakimi A, Altrock P, Mulé J, Manley B | 2023 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Highplex FL | HALO |
| Adrenal cortex size and homeostasis are regulated by gonadal hormones via androgen receptor/β-catenin signaling crosstalk | Lyraki R, Grabek A, Tison A, Weerasinghe-Arachchige L, Peitzsch M, Bechman N, Youssef S, de Bruin A, Bakker E, Claessens F, Chaboissier M, Schedl A | 2023 | Disease Models & Mechanisms | Other | FISH, Highplex FL | HALO |
| Multi-organ landscape of therapy-resistant melanoma | Liu S, Dharanipragada P, Lomeli S, Wang Y, Zhang X, Yang Z, Lim R, Dumitras C, Scumpia P, Dubinett S, Moriceau G, Johnson D, Moschos S, Lo R | 2023 | Nature Medicine | Oncology, Immuno-oncology | Highplex FL | HALO |
| Somatic mutation profiling, tumor-infiltrating leukocytes, tertiary lymphoid structures and PD-L1 protein expression in HER2-amplified colorectal cancer | Liu X, Kou Z, Zhang H, Dong J, Zhang J, Peng Y, Ma S, Liang L, Meng X, Zhou Y, Yang J | 2023 | PeerJ | Immuno-oncology | Highplex FL | HALO |
| Signal-transducing adaptor protein 1 (STAP1) in microglia promotes the malignant progression of glioma | Yang X, Ji C, Qi Y, Huang J, Hu L, Zhou Y, Zou L, Xia Y, Tan F, Yao Y, Chen D | 2023 | Research Square | Oncology | Highplex FL | HALO |
| Intracranial injection of natural killer cells engineered with a HER2-targeted chimeric antigen receptor in patients with recurrent glioblastoma | Burger M, Forster M, Romanski A, Straßheimer F, Macas J, Zeiner P, Steidl E, Herkt S, Weber K, Schupp J, Lun J, Strecker M, Wlotzka K, Cakmak P, Opitz C, George R, Mildenberger I, Nowakowska P, Zhang C, Röder J, Müller E, Ihrig K, Langen K, Rieger M, Herrmann E, Bonig H, Harter P, Reiss Y, Hattingen E, Rödel F, Plate K, Tonn T, Senft C, Steinbach J, Wels W | 2023 | Neuro-Oncology | Immuno-oncology | Highplex FL | HALO |
| Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis | Conroy L, Clarke H, Allison D, Valenca S, Sun Q, Hawkinson T, Young L, Ferreira J, Hammonds A, Dunne J, McDonald R, Absher K, Dong B, Bruntz R, Markussen K, Juras J, Alilain W, Liu J, Gentry M, Angel P, Waters C, Sun R | 2023 | Nature Communications | Fibrosis | Multiplex IHC, Highplex FL | HALO |
| Spatial analysis with SPIAT and spaSim to characterize and simulate tissue microenvironments | Feng Y, Yang T, Zhu J, Li M, Doyle M, Ozcoban V, Bass G, Pizzolla A, Cain L, Weng S, Pasam A, Kocovski N, Huang Y, Keam S, Speed T, Neeson P, Pearson R, Sandhu S, Goode D, Trigos A | 2023 | Nature Communications | Other | Highplex FL | HALO |
| High expression ITGA2 affects the expression of MET,PD-L1, CD4 and CD8 with the immune microenvironment in pancreatic cancer patients | Jin L, Duan Y, Li Z, Hu J, Shi H, Su Z, Li Z, Du B, Chen Y, Tan Y | 2023 | Research Square | Oncology | Highplex FL | HALO |
| Tumor-derived semaphorin 4A improves PD-1–blocking antibody efficacy by enhancing CD8+ T cell cytotoxicity and proliferation | NAITO Y, KOYAMA S, MASUHIRO K, HIRAI T, UENAMI T, INOUE T, OSA A, MACHIYAMA H, WATANABE G, SAX N, VILLA J, KINUGASA-KATAYAMA Y, NOJIMA S, YAGA M, HOSONO Y, OKUZAKI D, SATOH S, TSUDA T, NAKANISHI Y, SUGA Y, MORITA T, FUKUSHIMA K, NISHIDE M, SHIROYAMA T, MIYAKE K, IWAHORI K, HIRATA H, NAGATOMO I, YANO Y, TAMIYA M, KUMAGAI T, TAKEMOTO N, INOHARA H, YAMASAKI S, YAMASHITA K, AOSHI T, AKBAY E, HOSEN N, SHINTANI Y, TAKAMATSU H, MORI M, TAKEDA Y, KUMANOGOH A | 2023 | Science Advances | Immuno-oncology | Multiplex IHC, Highplex FL | HALO |
| Sequential pembrolizumab cooperates with platinum/5FU to remodel the tumor immune microenvironment in advanced gastric cancer: A phase II chemoimmunotherapy trial | Klempner S, Lee J, Mehta A, An M, Min B, Heo Y, Parikh M, Bi L, Cristescu R, Lee H, Kim T, Lee S, Moon J, Park R, Strickland M, Park W, Kang W, Kim K, Kim S | 2023 | Research Square | Immuno-oncology | Classifier, Highplex FL | HALO |
| An Fc-muted bispecific antibody targeting PD-L1 and 4-1BB induces antitumor immune activity in colorectal cancer without systemic toxicity | Cheng L, Zhu M, Gao Y, Liu W, Yin W, Zhou P, Zhu Z, Niu L, Zeng X, Zhang D, Fang Q, Wang F, Zhao Q, Zhang Y, Shen G | 2023 | Cellular and Molecular Biology Letters | Immuno-oncology | Multiplex IHC, Highplex FL | HALO |
| Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsis | Zhang S, Xu Q, Shi L, Li S, Wang W, Wang Q, Lu L, Xiao H, Wang J, Li F, Liang Y, Gong S, Peng H, Zhang Z, Tang G | 2023 | Signal Transduction and Targeted Therapy | Immunology | Highplex FL | HALO |
| Activation of IGFBP4 via unconventional mechanism of miRNA attenuates metastasis of intrahepatic cholangiocarcinoma | Tao L, Wang Y, Shen Z, Cai J, Zheng J, Xia S, Lin Z, Wan Z, Qi H, Jin R, Wang L, Xu J, Liang X | 2023 | Hepatology International | Oncology | Highplex FL | HALO |
| Characterization of intratumoral tertiary lymphoid structures in pancreatic ductal adenocarcinoma: cellular properties and prognostic significance | Zou X, Lin X, Cheng H, Chen Y, Wang R, Ma M, Liu Y, Dai Z, Tasiheng Y, Yan Y, Hou Q, Ding F, Chen H, Yu X, Wang X, Liu C | 2023 | Journal for ImmunoTherapy of Cancer | Immuno-oncology | Highplex FL | HALO, HALO AI |
| Trackable Intratumor Microdosing and Spatial Profiling Provide Early Insights into Activity of Investigational Agents in the Intact Tumor Microenvironment | Derry J, Burns C, Frazier J, Beirne E, Grenley M, DuFort C, Killingbeck E, Leon M, Williams C, Gregory M, Houlton J, Clayburgh D, Swiecicki P, Huszar D, Berger A, Klinghoffer R | 2023 | Cellular and Molecular Biology Letters | Oncology | Highplex FL | HALO, HALO AI |
| A cellular and molecular spatial atlas of dystrophic muscle | Stec M, Su Q, Adler C, Zhang L, Golann D, Khan N, Panagis L, Villalta S, Ni M, Wei Y, Walls J, Murphy A, Yancopoulos G, Atwal G, Kleiner S, Halasz G, Sleeman M | 2023 | Proceedings of the National Academy of Sciences | Myology | ISH/FISH, Highplex FL | HALO |
| Stem cell-like T cell depletion in the recurrent head and neck cancer immune microenvironment | Chen L, Lee N, Sarkar R, Katabi N, Li Y, Morris L, Wong R, Sherman E, Reis-Filho J, Hollmann T, Riaz N | 2023 | OncoImmunology | Immuno-oncology | Spatial Analysis, Highplex FL | HALO |
| TSLP/dendritic cell axis promotes CD4+ T cell tolerance to the gut microbiome | Messerschmidt J, Azin M, Dempsey K, Demehri S | 2023 | JCI Insight | Immunology | Highplex FL | HALO |
| Poor outcome in postpartum breast cancer patients is associated with distinct molecular and immunological features | Lefrère H, Moore K, Floris G, Sanders J, Seignette I, Bismeijer T, Peters D, Broeks A, Hooijberg E, Van Calsteren K, Neven P, Warner E, Peccatori F, Loibl S, Maggen C, Han S, Jerzak K, Annibali D, Lambrechts D, de Visser K, Wessels L, Lenaerts L, Amant F | 2023 | Clinical Cancer Research | Oncology | Classifier, Highplex FL | HALO, HALO AI |
| Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosis | Burkard T, Herrero San Juan M, Dreis C, Kiprina A, Namgaladze D, Siebenbrodt K, Luger S, Foerch C, Pfeilschifter J, Weigert A, Radeke H | 2023 | Clinical and Translational Medicine | Oncology, immunology | Classifier, Spatial Analysis, Highplex FL, TMA | HALO |
| An intestinal Th17 subset is associated with inflammation in Crohn’s Disease and activated by adherent-invasive Escherichia coli (AIEC) | Paroni M, Leccese G, Ranzani V, Moschetti G, Chiara M, Perillo F, Ferri S, Clemente F, Noviello D, Conforti FS, Ferrero S, Karnani B, Bosotti R, Vasco C, Curti S, Crosti MC, Gruarin P, Rossetti G, Conte MP, Vecchi M, Pagani M, Landini P, Facciotti F, Abrignani S, Caprioli F, Geginat J | 2023 | Journal of Crohn's and Colitis | Immunology | Highplex FL | HALO |
| Single-cell characterization of pulmonary nodules implicates suppression of immunosurveillance across early stages of lung adenocarcinoma | Yanagawa J, Tran L, Salehi-Rad R, Lim R, Dumitras C, Fung E, Wallace W, Prosper A, Fishbein G, Shea C, Hong R, Kahangi B, Deng J, Gower A, Liu B, Campbell J, Mazzilli S, Beane J, Kadara H, Lenburg M, Spira A, Aberle D, Krysan K, Dubinett S | 2023 | Cancer Research | Immuno-oncology | Highplex FL | HALO |
| The CD73 is induced by TGF-β1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanoma | Giraulo C, Turiello R, Orlando L, Leonardelli S, Landsberg J, Belvedere R, Rolshoven G, Muller C, Holzel M, Morello S | 2023 | Biomedicine & Pharmacotherapy | Oncology | Highplex FL, TMA | HALO, HALO AI |
| Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists | Harms P, Frankel T, Moutafi M, Rao A, Rimm D, Taube J, Thomas D, Chang M, Pantanowitz L | 2023 | Modern Pathology | Review | Spatial Analysis, Highplex FL | HALO |
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Module to assist in the segmentation of TMA spots for further analysis.
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Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.
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Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.
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