Before Markup

HALO® Module

Highplex FL

Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane.  With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya PhenoImager (Phenoptics), PhenoCycler (CODEX®), Vectra® 3, and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Lunaphore (OME.TIFF images from the COMET™ Platform), Ultivue (FixVUE™, FlexVUE™, U-Vue® Panels and InSituPlex® Technology), Standard BioTools (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Depicting the Cellular Architecture of the Tumour Microenvironment by Integrating Hyperplex Immunofluorescence and Automated Image Analysis

Download our scientific poster in collaboration with Lunaphore to learn more about how our Highplex FL module was used in the field.

Qualitative and Quantitative Evaluation of the Tissue Micro-Environment by High-Resolution 17-Plex Immunofluorescence Reveals Distinct Populations​

Download our scientific poster in collaboration with RareCyte to learn more about how our Highplex FL module was used to aid workflows.

Depicting TME Cellular Architecture Using Hyperplex IF and Automated Image Analysis

Download our eBook in collaboration with Lunaphore to learn more about how Pharma Services depicted TME cellular architecture using hyperplex IF and automated image analysis.

Qualitative and Quantitative Evaluation of the Tissue Micro-Environment by High-Resolution 17-Plex Immunofluorescence Reveals Distinct Populations

Check out this eBook to see how AI-based nuclear segmentation and tissue classification can streamline cell phenotyping across tissue compartments and samples.

Sequential Same Slide Multiplex IF and H&E Staining for Combined Phenotypic and Morphological Characterization of FFPE Tissue Sections

Read this eBook for an introduction to a sequential staining and image analysis workflow that leverages a terminal H&E stain for the delineation of tissue architectural regions in subsequent HALO image analysis.

Introduction of a Robust Workflow for the Whole-Slide Acquisition and Co-Registration of Multiplex Immunofluorescence Tissue Images for Analysis of a 9-Color, 8-Marker Immunophenotyping Assay

Download our eBook to learn about a HALO workflow for co-registration and cellular analysis of immunofluorescence images from cyclically stained and imaged slides.

Publication Spotlight

The table below includes publications that cite the Highplex FL module. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO ModulesProduct
Cannabinoid control of gingival immune activation in chronically SIV-infected rhesus macaques involves modulation of the indoleamine-2,3-dioxygenase-1 pathway and salivary microbiomeMcDew-White M, Lee E, Alvarez X, Sestak K, Ling B, Byrareddy S, Okeoma C, Mohan M2022EBioMedicineInfectious DiseaseArea Quantification, Cytonuclear, Highplex FLHALO
ACE2-IgG1 fusions with improved in vitro and in vivo activity against SARS-CoV-2Iwanaga N, Cooper L, Rong L, Maness N, Beddingfield B, Qin Z, Crabtree J, Tripp R, Yang H, Blair R, Jangra S, Garcia-Sastre A, Schotsaert M, Chandra S, Robinson J, Srivastava A, Rabito F, Qin X, Kolls J2022iScienceInfectious DiseaseHighplex FLHALO AI
Tumor MHC Class I Expression Associates with Intralesional Interleukin-2 Response in MelanomaPourmaleki M, Jones C, Ariyan C, Zeng Z, Pirun M, Navarrete D, Li Y, Zhang M, Nandakumar S, Campos C, Nadeem S, Klimstra D, Temple-Oberle C, Brenn T, Kipson E, Schenk K, Stein J, Taube J, White M, Traweek R, Wargo J, Kirkwood J, Gasmi B, Goff S, Corwin A, McDonough E, Ginty F, Callahan M, Schietinger A, Socci N, Mellinghoff I, Hollmann T2022Cancer Research ImmunologyImmuno-oncologyHighplex FLHALO
Progranulin mediates immune evasion of pancreatic ductal adenocarcinoma through regulation of MHCI expressionCheung P, Yang J, Fang R, Borgers A, Krengel K, Stoffel A, Althoff K, Yip C, Siu E, Ng L, Lang K, Cham L, Engel D, Soun C, Cima I, Scheffler B, Striefler J, Sinn M, Bahra M, Pelzer U, Oettle H, Markus P, Smeets E, Aarntzen E, Savvatakis K, Liffers S, Lueong S, Neander C, Bazarna A, Zhang X, Paschen A, Crawford H, Chan A, Cheung S, Siveke J2022Nature CommunicationsOncologyCytonuclear, Spatial Analysis, Highplex FLHALO
Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancerDu S, Qian J, Tan S, Li W, Liu P, Zhao J, Zeng Y, Xu L, Wang Z, Cai J2022Cancer LettersOncologyArea Quantification, Cytonuclear, Spatial Analysis, Highplex FLHALO
Understanding the impact of chemotherapy on the immune landscape of high-grade serous ovarian cancerVanguri R, Benhamida J, Young J, Li Y, Zivanovic O, Chi D, Snyder A, Hollmann T, Mager K2022Gynecologic Oncology ReportsOncologyClassifier, Cytonuclear, Highplex FLHALO
New Fully Automated Preparation of High Apparent Molar Activity 68Ga-FAPI-46 on a Trasis AiO PlatformDa Pieve C, Braga M, Turton D, Valla F, Cakmak P, Plate K, Kramer-Marek G2022MoleculesOtherCytonuclear, Highplex FLHALO
Retrospective analysis of the efficacy of adjuvant cytokine-induced killer cell immunotherapy combined with chemotherapy in colorectal cancer patients after surgeryLi X, Zhou H, Huang W, Wang X, Meng M, Hou Z, Liao L, Tang W, Xie Y, Wang R, Yu H, Wang L, Zhu H, Wang W, Tan J, Li R2022Clinical & Translational ImmunologyOncologyCytonuclear, Highplex FLHALO
Pancreas resident macrophage-induced fibrosis has divergent roles in pancreas inflammatory injury and PDACBaer J, Zuo C, Kang L, Alarcon de la Lastra A, Bocherding N, Knolhoff B, Bogner S, Zhu Y, Lewis M, Zhang N, Kim K, Fields R, Mills J, Ding L, Randolph G, DeNardo D2022bioRxivFibrosisClassifier, Area Quantification, Cytonuclear, Multiplex IHC, Highplex FL, Figure MakerHALO
Genomic and single-cell landscape reveals novel drivers and therapeutic vulnerabilities of transformed cutaneous T-cell lymphomaSong X, Chang S, Seminario-Vidal L, de Mingo Pulido A, Tordesillas L, Song X, Reed R, Harkins A, Whiddon S, Nguyen J, Segura C, Zhang C, Yoder S, Sayegh Z, Zhao Y, Messina J, Harro C, Zhang X, Conejo-Garcia J, Berglund A, Sokol L, Zhang J, Rodriguez P, Mule J, Futreal A, Tsai K, Chen P2022Cancer DiscoveryOncologyCytonuclear, Highplex FL, TMAHALO
Tumor immune cell clustering and its association with survival in African American women with ovarian cancerWilson C, Soupir A, Thapa R, Creed J, Nguyen J, Segura C, Gerke T, Schildkraut J, Peres L, Fridley B2022PLOS Computational BiologyCytonuclear, Highplex FLHALO
Immunologic Gene Signature Analysis Correlates Myeloid Cells and M2 Macrophages with Time to Trabectedin Failure in Sarcoma PatientsSchroder B, Zhang Y, Smythe K, Desai P, Thomas A, Viveiros P, Alexiev B, Obeidin F, Chen E, Cranmer L, Wagner M, Jones R, Campbell J, Pierce R, He Q, Pollack S2022CancersOncologyCytonuclear, Highplex FLHALO
Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 ExpressionCarossino M, Kenney D, O'Connell A, Montarnaro P, Tseng A, Gertje H, Grosz K, Ericsson M, Huber B, Kurnick S, Subramaniam S, Kirkland T, Walker J, Francis K, Klose A, Paragas N, Bosmann M, Saeed M, Balasuriya U, Douam F, Crossland N2022VirusesInfectious DiseaseClassifier, Area Quantification, Highplex FLHALO
Multi-modal molecular imaging maps the correlation between tumor microenvironments and nanomedicine distributionStrittmatter N, Moss J, Race A, Sutton D, Canales J, Ling S, Wong E, Wilson J, Smith A, Howes C, Bunch J, Barry S, Goodwin R, Ashford M2022TheranosticsImmuno-oncologyHighplex FLHALO, HALO AI
An anti-PD-1ñGITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapyChan S, Belmar N, Ho S, Rogers B, Stickler M, Graham M, Lee E, Tran N, Zhang D, Gupta P, Sho M, MacDonough T, Woolley A, Kim H, Zhang H, Liu W, Zheng P, Dezso Z, Halliwill K, Ceccarelli M, Rhodes S, Thakur A, Forsyth C, Xiong M, Tan S, Iyer R, Lake M, Digiammarino E, Zhou L, Bigelow L, Longenecker K, Judge R, Liu C, Trumble M, Remis J, Fox M, Cairns B, Akamatsu Y, Hollenbaugh D, Farding F, Alvarez H2022Nature CancerImmuno-oncologyArea Quantification, Cytonuclear, Highplex FLHALO
ZEB1 transcription factor promotes immune escape in melanomaPlaschka M, Benboubker V, Grimont M, Berthet J, Tonon L, Lopez J, Le-Bouar M, Balme B, Tondeau G, de la Fouchardiere A, Larue L, Puisieux A, Grinberg-Bleyer Y, Bendriss-Vermare N, Dubois B, Caux C, Dalle S, Caramel J2022Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Multiplex IHC, Highplex FLHALO
Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretionVeprik A, Denwood G, Liu D, Bakar R, Morfin V, McHugh K, Tebeka N, Vetterli L, Yonova-Doing E, Gribble F, Reimann F, Hoehn K, Hemsley P, Ahnfelt-Ronne J, Rorsman P, Zhang Q, Wet H, Cantley J2022Communications BiologyMetabolismCytonuclear, Highplex FLHALO
Extracellular matrix proteins regulate NK cell function in peripheral tissuesBunting M, Vyas M, Requesens M, Langenbucher A, Schiferle E, Manguso R, Lawrence M, Demehri S2022Science AdvancesDermatologyCytonuclear, Highplex FLHALO
Multiplex Immunohistochemical Phenotyping of T Cells in Primary Prostate CancerOzbek B, Ertunc O, Erickson A, Vidal I, Gomes-Alexandre C, Guner G, Hicks J, Jones T, Taube J, Sfanos K, Yegnasubramanian S, DeMarzo A2022The ProstateImmuno-oncologyClassifier, Cytonuclear, Highplex FL, Serial Section, Registration, Figure Maker, TMAHALO
Adrenal tropism of SARS-CoV-2 and adrenal findings in a post-mortem case series of patients with severe fatal COVID-19Paul T, Ledderose S, Bartsch H, Sun N, Soliman S, Markl B, Ruf V, Herms J, Stern M, Keppler O, Delbridge C, Muller S, Piontek G, Kimoto Y, Schreiber F, Williams T, Neumann J, Knosel T, Schulz H, Spallek R, Graw M, Kirchner T, Walch A, Rudelius M2022Nature CommunicationsInfectious DiseaseCytonuclear, Highplex FLHALO
Spatially mapping the immune landscape of melanoma using imaging mass cytometryMoldoveanu D, Ramsay L, Lajoie M, Anderson-Trocme L, Lingrand M, Berry D, Perus L, Wei Y, Moraes C, Alkallas R, Rajkumar S, Zuo D, Dankner M, Xu E, Bertos N, Najafabadi H, Gravel S, Costantino S, Richer M, Lund A, Del Rincon S, Spatz A, Miller W, Jamal R, Lapointe R, Mes-Masson A, Turcotte S, Petrecca K, Dumitra S, Meguerditchian A, Richardson K, Tremblay F, Wang B, Chergui M, Guiot M, Watters K, Stagg J, Quail D, Mihalcioiu C, Meterissian S, Watson I2022Science ImmunologyOncologyHighplex FLHALO
Improved cognitive impairments by silencing DMP1 via enhancing the proliferation of neural progenitor cell in Alzheimer-like miceZhao H, Wei J, Du Y, Chen P, Liu X, Liu H2022Aging CellNeuroscienceCytonuclear, Highplex FLHALO
Peritumoral TIGIT+CD20+†B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancerLiu H, Wu J, Xu X, Wang H, Zhang C, Yin S, He Y2022International ImmunopharmacologyOncologyCytonuclear, Spatial Analysis, Highplex FLHALO
Subpopulations of cancer-associated fibroblasts link the prognosis and metabolic features of pancreatic ductal adenocarcinomaHu B, Wu C, Mao H, Gu H, Dong H, Yan J, Qi Z, Yuan L, Dong Q, Long J2022Annals of Translational MedicineOncologyCytonuclear, Highplex FLHALO
Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infectionKenney D, O'Connell A, Turcinovic J, Montanaro P, Hekman R, Tamura T, Berneshawi A, Cafiero T, Abdullatif S, Blum B, Goldstein S, Heller B, Gertje H, Bullitt E, Trachtenberg A, Chavez E, Nono E, Morrison C, Tseng A, Sheikh A, Kurnick S, Grosz K, Bosmann M, Ericsson M, Huber B, Saeed M, Balazs A, Francis K, Klose A, Paragas N, Campbell J, Connor J, Emili A, Crossland N, Ploss A, Douam F2022Cell ReportsInfectious DiseaseArea Quantification, Highplex FLHALO
SPOP promotes cervical cancer progress by inducing PD-1 move away from PD-L1 in spatial localizationWu J, Wu Y, Guo Q, Chen S, Wang S, Zhu J, Wu X, Ju X2022Research SquareOncologyCytonuclear, Spatial Analysis, Highplex FL, TMAHALO
Pilot Trial of Arginine Deprivation Plus Nivolumab and Ipilimumab in Patients with Metastatic Uveal MelanomaKraehenbuehl L, Holland A, Armstrong E, O'Shea S, Mangarin L, Chekalil S, Johnston A, Bomalaski J, Erinjeri J, Barker C, Francis J, Wolchok J, Merghoub T, Shoushtari A2022CancersOncologyCytonuclear, Highplex FLHALO
Nitric oxide favours tumour-promoting inflammation through mitochondria-dependent and -independent actions on macrophagesDrehmer D, Luiz J, Hernandez C, Alves-Filho J, Hussell T, Townsend P, Moncada S2022Redox BiologyOncologyHighplex FLHALO
CD4+†T helper 2 cells suppress breast cancer by inducing terminal differentiationBoieri M, Malishkevich A, Guennoun R, Marchese E, Kroon S, Trerice K, Awad M, Park J, Iyer S, Kreuzer J, Haas W, Rivera M, Demehri S2022Journal of Experimental MedicineImmuno-oncologyCytonuclear, Highplex FLHALO
Higher proportions of CD39+ tumorresident cytotoxic T cells predict recurrence-free survival in patients with stage III melanoma treated with adjuvant immunotherapyAttrill G, Owen C, Ahmed T, Vergara I, Colebatch A, Conway J, Nahar K, Thompson J, da Silva I, Carlino M, Menzies A, Lo S, Palendira U, Scolyer R, Long G, Wilmott J2022Journal of Immuno Therapy of CancerImmuno-oncologyCytonuclear, Spatial Analysis, Highplex FLHALO
Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational studyShui I, Liu X, Zhao Q, Kim S, Sun Y, Yearley J, Choudhury T, Webber A, Krepler C, Cristescu R, Lee J2022Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Highplex FLHALO
Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate speciesFears A, Beddingfield B, Chirichella N, Slisarenko N, Kileen S, Redmann R, Goff K, Spencer S, Picou B, Golden N, Midkiff C, Bush D, Branco L, Boisen M, Gao H, Montefiori D, Blair R, Doyle-Meyers L, Russel-Lodrigue K, Maness N, Roy C2022PLOS PathogensInfectious DiseaseHighplex FLHALO
gp96 Expression in Gliomas and Its Association with Tumor Malignancy and T Cell Infiltrating LevelLi C, Wang Y, Long L, Zhang P, Zhang Y, Ji N2022Hindawi Journal of OncologyImmuno-oncologySpatial Analysis, Highplex FL, Figure MakerHALO
Multiplexed immunofluorescence analysis of CAFmarkers, EZH2 and FOXM1 in Gastric Tissue: Associations with Clinicopathological Parameters and Clinical OutcomesSun H, Wang X, Wang X, Tan C, Weng W, Zhang M, Ni S, Wang L, Huang D, Sheng W, Xu M2022Research SquareOncologyCytonuclear, Highplex FLHALO
Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrixKay E, Paterson K, Riero-Domingo C, Sumpton D, Dabritz J, Tardito S, Boldrini C, Hernandez-Fernaud J, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson L, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson R, Park M, Kirschner K, Miller C, Kamphorst J, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S2022Nature MetabolismOncology, MetabolismCytonuclear, Multiplex IHC, Highplex FLHALO
Integrated single-cell analyses decode the developmental landscape of the human fetal spineYu H, Tang D, Wu H, Li C, Lu Y, He F, Zhang X, Yang Y, Shi W, Hu W, Zeng Z, Dai W, Ou M, Dai Y2022iScienceOtherHighplex FLHALO
Establishing a multiplex imaging panel to study T cell development in the thymus in mouseAllam A, Russell S2022STAR ProtocolsImmunologyHighplex FLHALO
Developing T cells form an immunological synapse for passage through the ?-selection checkpointAllam A, Charnley M, Pham K, Russel S2022Journal of Cell BiologyImmunologyHighplex FLHALO
Endothelial cell death after ionizing radiation does not impair vascular structure in mouse tumor modelsKaeppler J, Chen J, Buono M, Vermeer J, Kannan P, Cheng W, Voukantsis D, Thompson J, Hill M, Allen D, Gomes A, Kersemans V, Kinchesh P, Smart S, Buffa F, Nerlov C, Muschel R, Markelc B2022EMBO ReportsOncologySpatial Analysis, Highplex FLHALO
Non-canonical EphA2 activation underpins PTEN-mediated metastatic migration and poor clinical outcome in prostate cancerSachdeva A, Hart C, Kim K, Tawadros T, Oliveira P, Shanks J, Brown M, Clarke N2022British Journal of CancerOncologyHighplex FLHALO
Approaches to spatially resolving the tumour immune microenvironment of hepatocellular carcinomaMarsh-Wakefield F, Ferguson A, Liu K, Santhakumar C, McCaughan G, Palendira U2022Therapeutic Advances in Medical OncologyReviewMultiplex IHC, Spatial Analysis, Highplex FLHALO
Tau Pathology in Chronic Traumatic Encephalopathy is Primarily NeuronalButler M, Dixon E, Stein T, Alvarez V, Huber B, Buckland M, McKee A, Cherry J2022Journal of Neuropathology & Experimental NeurologyNeuroscienceMultiplex IHC, Spatial Analysis, Highplex FLHALO, HALO AI
Dynamic and adaptive cancer stem cell population admixture in colorectal neoplasiaVasquez E, Nasreddin N, Valbuena G, Mulholland E, Belnoue-Davis H, Eggington H, Schenck R, Wouters V, Wirapati P, Gilroy K, Lannagan R, Flanagan D, Nujumudeen A, Omwenga S, McCorry A, Easton A, Koelzer V, East J, Morton D, Trusolino L, Maughan T, Campbell A, Loughrey M, Dunne P, Tsantoulis P, Juels D, Tejpar S, Sansom O, Leedham S2022Cell Stem CellOncologyHighplex FL, RegistrationHALO
Knockdown of ZBTB11 impedes R-loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancerChen L, Liu Z, Tang H, Zhou Z, Chen J, Ma Z, Deng M, Li X, Wu Y, Zheng L, Zhou L, Zheng X, Liu Z2022Cell ProliferationOncologyMultiplex IHC, Highplex FL, Figure MakerHALO
LncRNA scaRNA2 bridges DNA end-resection to homologous recombination repair mediated chemoradioresistanceYang Y, Chen Y, Shen H, Liu T, Cao K, Wan Z, Du Z, Wang H, Yu Y, Ma S, Li B, Zhang W, Cai J, Gao F2022Research SquareOncologyHighplex FL, TMAHALO
Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancersXu Y, Wei Z, Feng M, Zhu D, Mei S, Wu Z, Feng Q, Chang W, Ji M, Liu C, Zhu Y, Shen L, Yang F, Chen Y, Feng Y, Xu J, Zhu D2022Cell ReportsOncology, Immuno-oncologyMultiplex IHC, Highplex FLHALO
Transcriptional and functional analyses of neoantigen-specific CD4 T cells during a profound response to anti-PD-L1 in metastatic Merkel cell carcinomaChurch C, Pulliam T, Longino N, Park S, Smythe K, Makarov V, Riaz N, Jing L, Amezquita R, Campbell J, Gottardo R, Pierce R, Choi J, Chan T, Koelle D, Nghiem P2022Journal for ImmunoTherapy of CancerOncology, Immuno-oncologyHighplex FLHALO
Modelling aggressive prostate cancers of young men in immune-competent mice, driven by isogenic†Trp53†alterations and†Pten†lossMejia-Hernandez J, Keam S, Saleh R, Muntz F, Fox S, Byrne D, Kogan A, Pang L, Huynh J, Litchfield C, Caramia F, Lozano G, He H, You J, Sandhu S, Williams S, Haupt Y, Haupt S2022Cell Death & DiseaseOncologyMultiplex IHC, Highplex FL, Figure MakerHALO
In vivo tumor immune microenvironment phenotypes correlate with inflammation and vasculature to predict immunotherapy responseSahu A, Kose K, Kraehenbuehl L, Byers C, Holland A, Tembo T, Santella A, Alfonso A, Li M, Cordova M, Gill M, Fox C, Gonzalez S, Kumar P, Wang A, Kurtansky N, Chandrani P, Yin S, Mehta P, Navarrete-Dechent C, Peterson G, King K, Dusza S, Yang N, Liu S, Phillips W, Guitera P, Rossi A, Halpern A, Deng L, Pulitzer M, Marghoob A, Chen C, Merghoub T, Rajadhyaksha M2022Nature CommunicationsImmuno-oncologyHighplex FL, Figure MakerHALO
In†vivo†G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-? secretion by natural killer cellsZhao X, Peng T, Cao X, Hou Y, Li R, Han T, Fan Z, Zhao M, Chang Y, Chen H, Li C, Huang X2022Cell ReportsImmunologyHighplex FLHALO
Phase I/II clinical trial of a helper peptide vaccine plus PD-1 blockade in PD-1 antibody-naÔve and PD-1 antibody-experienced patients with melanoma (MEL64)Vavolizza R, Petroni G, Mauldin I, Chianese-Bullock K, Olson W, Smith K, Dengel L, Haden K, Grosh W, Kaur V, Varhegyi N, Gaughan E, Slingluff C2022Journal for ImmunoTherapy of CancerImmuno-oncologyHighplex FLHALO
Unveiling the tumor immune microenvironment of organ-specific melanoma metastatic sitesConway J, Rawson R, Lo S, Ahmed T, Veregara I, Gide T, Attrill G, Carlino M, Saw R, Thompson J, Spillane A, Shannon K, Shivalingam B, Menzies A, Wilmott J, Long G, Scolyer R, da Silva I 2022Journal for ImmunoTherapy of CancerOncologyClassifier, Spatial Analysis, Highplex FLHALO
Intratumoral administration of CD1c (BDCA-1)+†and CD141 (BDCA-3)+†myeloid dendritic cells in combination with talimogene laherparepvec in immune checkpoint blockade refractory advanced melanoma patients: a phase I clinical trialSchwarze J, Tijtgat J, Awada G, Cras L, Vasaturo A, Bagnall C, Forsyth R, Dufait I, Tuyaerts S, Riet I, Neyns B2022Journal for ImmunoTherapy of CancerOncology, Immuno-oncologyHighplex FLHALO
TIE2-high cervical cancer cells promote tumor angiogenesis by upregulating TIE2 and VEGFR2 in endothelial cellsTan S, Chen Y, Du S, Li W, Liu P, Zhao J, Yang P, Cai J, Gao R, Wang Z2022Translational OncologyOncologyArea Quantification, Highplex FLHALO
Proliferative tumor infiltrating lymphocytes abundance within microenvironment impact clinical outcome in cutaneous B-cell lymphomasMenguy S, Prochazkova-Carolotti M, Azzi-Martin L, Ferte T, Bresson-Bepoldin L, Rey C, Vergier B, Merlio J, Beylot-Barry M, Pham-Ledard A2022Journal of Investigative DermatologyImmuno-oncologyClassifier, Highplex FLHALO
USP14 promotes tryptophan metabolism and immune suppression by stabilizing IDO1 in colorectal cancerShi D, Wu X, Jian Y, Wang J, Huang C, Mo S, Li Y, Li F, Zhang C, Zhang D, Zhang H, Huang H, Chen X, Wang Y, Lin C, Liu G, Song L, Liao W2022Nature CommunicationsOncology, DermatologyCytonuclear, Highplex FLHALO, HALO AI
Single Cell RNA-Seq Identifies Immune-Related Prognostic Model and Key Signature-SPP1 in Pancreatic Ductal AdenocarcinomaChen K, Wang Q, Liu X, Wang F, Ma Y, Zhang S, Shao Z, Yang Y, Tian X2022GenesOncologyCytonuclear, Highplex FLHALO
Relaxin/insulin-like family peptide receptor 4 (Rxfp4) expressing hypothalamic neurons Q8 modulate food intake and preference in miceLewis J, Woodward O, Nuzzaci D, Smith C, Adriaenssens A, Billing L, Brighton C, Phillips B, Tadross J, Kinston S, Ciabatti E, Gottgens B, Tripodi M, Mornigold D, Baker D, Gribble F, Reimann F2022Molecular MetabolismMetabolismISH/FISH, Highplex FLHALO
Attenuation of relapsing fever neuroborreliosis in mice by IL-17A blockadeCheng M, Xu J, Ding K, Liu H2022Proceedings of the National Academy of SciencesInfectious DiseaseHighplex FLHALO
Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin systemWeigert A, Zheng X, Nenzel A, Turkowski K, Gunther S, Strack E, Sirait-Fischer E, Elwakeel E, Kur I, Nikam V, Valasarajan C, Winter H, Wissgott A, Voswinkel R, Grimminger F, Brune B, Seeger W, Pullamsetti S, Savai R2022Nature CommunicationsOncologySpatial Analysis, Highplex FLHALO
Single-cell RNA-seq transcriptomic landscape of human and mouse islets and pathological alterations of diabetesChen K, Zhang J, Huang Y, Tian X, Yang Y, Dong A2022iScienceMetabolismCytonuclear, Highplex FLHALO
Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibodyMicalizzi D, Che D, Nicholson B, Edd J, Desai N, Lang E, Toner M, Maheswaran S, Ting D, Haber D2022Proceedings of the National Academy of SciencesOncologyMultiplex IHC, Highplex FLHALO
Single-cell RNA sequencing reveals cellular and molecular reprograming landscape of gliomas and lung cancer brain metastasesSun H, Li L, Lao I, Li X, Xu B, Cao Y, Jin W2022Clinical and Translational MedicineOncologyCytonuclear, Highplex FLHALO
Impacts of neoadjuvant chemoradiotherapy on the immune landscape of esophageal squamous cell carcinomaWen J, Fang S, Hu Y, Xi M, Weng Z, Pan C, Luo K, Ling Y, Lai R, Xie X, Lin X, Lin T, Chen J, Liu Q, Fu J, Yang H2022EBioMedicineImmuno-oncologyCytonuclear, Highplex FLHALO
Method To Visualize the Intratumor Distribution and Impact of Gemcitabine in Pancreatic Ductal Adenocarcinoma by Multimodal ImagingStrittmatter N, Richards F, Race A, Ling S, Sutton D, Nilsson A, Wallez Y, Barnes J, Maglennon G, Gopinathan A, Brais R, Wong E, Serra M, Atkinson J, Smith A, Wilson J, Hamm G, Johnson T, Dunlop C, Kaistha B, Bunch J, Sansom O, Takats Z, Andren P, Lau A, Barry S, Goodwin R, Jodrell D2022Analytical ChemistryOncologyClassifier, Highplex FLHALO, HALO AI
Xiaoyaosan ethyl acetate fraction alleviates depression-like behaviors in CUMS mice by promoting hippocampal neurogenesis via modulating the IGF-1R_/PI3K/Akt signaling pathwayZeng J, Ji Y, Luan F, Hu J, Rui Y, Liu Y, Rao Z, Liu R, Zeng N2022Journal of Experimental & Clinical Cancer ResearchNeuroscienceHighplex FLHALO
SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancerLi, T, Jin K, Li H, Ye L, Li P, Jiang B, Lin X, Liao Z, Zhang H, Shi S, Lin M, Fei Q, Xiao Z, Xu H, Liu L, Yu X, Wu W2022Cancer LettersImmuno-oncologyHighplex FL, TMAHALO
Presence of Tim3+ and PD-1+ CD8+ T cells identifies microsatellite stable colorectal carcinomas with immune exhaustion and distinct clinicopathological featuresKlapholz M, Drage M, Srivastava A, Anderson A2022The Journal of PathologyOncologyMultiplex IHC, Highplex FLHALO
Impact of HPV status on immune responses in head and neck squamous cell carcinomaQureshi H, Zhu X, Yang G, Steadele M, Pierce R, Futran N, Lee S, Mendez E, Houghton A2022Oral OncologyOncologyMultiplex IHC, Spatial Analysis, Highplex FLHALO
Developing a Nomogram for Preoperative Prediction of Cervical Cancer Lymph Node Metastasis by Multiplex ImmunofluorescenceWu J, Guo Q, Zhu J, Wu Y, Liang S Chen S, Wang S, Ju X, Wu X2022AuthoreaOncologySpatial Analysis, Highplex FL, TMAHALO
PERK reprograms hematopoietic progenitor cells to direct tumor-promoting myelopoiesis in the spleenLiu M, Wu C, Luo S, Hua Q, Chen H, Weng Y, Xu J, Lin H, Wang L, Li J, Zhu L, Guo Z, Zhuang S, Kang T, Zheng L2022Journal of Experimental MedicineOncologyHighplex FL, RegistrationHALO
Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancerRamos R, Missolo-Koussou Y, Gerber-Ferder Y, Bromley C, Bugatti M, Nunez N, Boari J, Richer W, Menger L, Denizeau J, Sedlik C, Caudana P, Kotsias F, Niborski L, Viel S, Bohec M, Lameiras S, Baulande S, Lesage L, Nicolas A, Meseure D, Vincent-Salomon A, Reyal F, Dutertre C, Ginhoux F, Vimeux L, Donnadieu E, Buttard B, Galon J, Zelenay S, Vermi W, Guermonprez P, Piaggio E, Helft J2022CellImmuno-oncologyClassifier, Spatial Analysis, Highplex FLHALO
A Phase 1/2 Trial Combining Avelumab and Trabectedin for Advanced Liposarcoma and LeiomyosarcomaWagner M, Zhang Y, Cranmer L, Loggers E, Black G, McDonnell S, Maxwell S, Johnson R, Moore R, Hermida de Viveriros P, Aicher L, Smythe K, He Q, Jones R, Pollack S2022Clinical Cancer ResearchOncologyHighplex FLHALO
Anatomic and functional mapping of human uterine innervationPinsard M, Mouchet N, Dion L, Bessede T, Bertrand M, Darai E, Bellaud P, Loget P, Mazaud-Guittot S, Morandi X, Leveque J, Lavoue V, Duraes M, Timoh K2022Fertility and SterilityOtherHighplex FLHALO
Neoantigen-specific CD4+ T cells in human melanoma have diverse differentiation states and correlate with CD8+ T cell, macrophage, and B cell functionVeatch J, Lee S, Shasha C, Singhi N, Szeto J, Moshiri A, Kim T, Smythe K, Kong P, Fitzgibbon M, Jesernig B, Bhatia S, Tykodi S, Hall E, Byrd D, Thompson J, Pillarisetty V, Duhen T, Houghton A, Newell E, Gottardo R, Riddell S2022Cancer CellImmuno-oncologySpatial Analysis, Highplex FLHALO
A molecular analysis of neural olfactory placode differentiation in human pluripotent stem cellsBricker R, Bhaskar U, Titone R, Carless M, Barberi T2022Stem Cells and DevelopmentOtherHighplex FL, Figure MakerHALO
PHGDH heterogeneity potentiates cancer cell dissemination and metastasisRossi M, Altea-Manzano P, Demicco M, Doglioni G, Bornes L, Fukano M, Vandekeere A, Cuadros A, Fernandez-Garcia J, Riera-Domingo C, Jauset C, Planque M, Alkan H, Nittner D, Zuo D, Broadfield L, Parik S, Pane A, Rizzollo F, Rinaldi G, Zhang T, Teoh S, Aurora A, Karras P, Vermeire I, Broekaert D, Van Elsen J, Knott M, Orth M, Demeyer S, Eelen G, Dobrolecki L, Bassez A BRussel T, Sotlar K, Lewis M, Bartsch H, Wuhrer M, van Veelen P, Carmeliet J, Cools J, Morrison S, Marine J, Lambrechts D, Mazzone M, Hannon G, Lunt S, Grunewald T, Park M, van Rheenen J, Fendt S2022NatureOncologyHighplex FLHALO
Potentiating adoptive cell therapy using synthetic IL-9 receptorsKalbasi A, Siurala M, Su L, Tariveranmoshabad M, Picton L, Ravikumar P, Li P, Lin J, Escuin-Ordinas H, Da T, Kremer S, Sun A, Castelli S, Agarwal S, Scholler J, Song D, Rommel P, Radaelli E, Young R, Leonard W, Ribas A, June C, Gargia K2022NatureImmuno-oncologyHighplex FLHALO
Tumor cells modulate macrophage phenotype in a novel in vitro co-culture model of the non-small cell lung cancer tumor microenvironmentPark J, Chandra R, Cai L, Ganguly D, Li H, Toombs J, Girard L, Brekken R, Minna J2022Journal of Thoracic OncologyImmuno-oncologyHighplex FLHALO
A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell CarcinomaRappold P, Vuong L, Leibold J, Chakiryan N, Curry M, Kuo F, Sabio E, Jiang H, Nixon B, Liu M, Berglund A, Silagy A, Mascareno A, Golkaram M, Marker M, Reising A, Savchenko A, Milholland J, Chen Y, Russo P, Coleman J, Reznik E, Manley B, Ostrovnaya I, Makarav V, DiNatale R, Blum K, Ma X, Chowell D, Li M, Solit D, Lowe S, Chan T, Motzer R, Voss M, Hakimi A2022Cancer DiscoveryOncologyHighplex FLHALO
Spatiotemporal expression pattern of Progesterone Receptor Component (PGRMC) 1 in endometrium from patients with or without endometriosis or adenomyosisThieffry C, Van Wynendaele M, Samain L, Tyteca D, Pierreux C, Marbaix E, Henriet P2022The Journal of Steroid Biochemistry and Molecular BiologyOtherISH/FISH, Highplex FLHALO, HALO AI
Neoadjuvant Therapy Induces a Potent Immune Response to Sarcoma, Dominated by Myeloid and B CellsGoff P, Riolobos L, LaFleur B, Spraker M, Seo Y, Smythe K, Campbell J, Pierce R, Zhang Y, He Q, Kim E, Schaub S, Kane G, Mantilla J, Chen E, Ricciotti R, Thompson M, Cranmer L, Wagner M, Loggers E, Jones R, Murphy E, Blumenschein W, McClanahan T, Earls J, Flanagan K, LaFranzo N, Kim T, Pollack S2022Clinical Cancer ResearchImmuno-oncologyHighplex FLHALO
Targeted RNA editing in brainstem alleviates respiratory dysfunction in a mouse model of Rett syndromeSinnamon J, Jacobson M, Yung J, Mandel G2022Proceedings of the National Academy of SciencesNeuroscienceHighplex FLHALO
Characterization of anti-CD79b/CD3 bispecific antibody, a potential therapy for B cell malignanciesWang J, Li C, He K, Kuang Z, Lu J, Yao Y, He F, Li N, Li L, Fu F, Wu Z, Zhou S, Kang D, Qiu X, Wu M, Liu Y, Cao X, Xu M, Chen B, Wu W, Guo F2022Cancer Immunology, ImmunotherapyImmuno-oncologyHighplex FLHALO
Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesisSabate-Perez A, Romero M, Sanchez-Fernandez-de-Landa P, Carobbio S, Mouratidis M, Sala D, Engel P, Villena J, Virtue S, Vidal-Puig A, Palacin M, Testar X, Zorzano A2022AutophagyMetabolismHighplex FLHALO, HALO AI
Epigallocatechin-3-gallate reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitisLi H, Qiao C, Zhao L, Jing Q, Xue D, Zhang Y2022Journal of Leukocyte BiologyImmunologyHighplex FLHALO
Notch signaling promotes mature T-cell lymphomagenesisGao X, Wang C, Abdelrahman S, Kady N, Murga-Zamalloa C, Gann P, Sverdlov M, Wolfe A, Polk A, Brown N, Bailey N, Inamdar K, Casavilca-Zambrano S, Montes J, Barrionuevo C, Taxa L, Reneau J, Siebel C, Maillard I, Wilcox R2022Cancer ResearchImmuno-oncologyHighplex FLHALO, HALO AI
Plasma CD27, a surrogate of the intratumoral CD27-CD70 interaction, correlates with immunotherapy resistance in renal cell carcinomaBenhamouda N, Sam I, Epaillard N, Gey A, Phan L, Pham H, Gruel N, Saldmann A, Pineau J, Hasan M, Quiniou V, Nevoret C, Verkarre V, Libri V, Mella S, Granier C, Broudin C, Ravel P, De Guillebon E, Mauge L, Helley D, Jabla B, Chaput N, Albiges L, Katsahian S, Adam J, Mejean A, Adotevi O, Vano Y, Oudard S, Tartour E2022Clinical Cancer ResearchOncologySpatial Analysis, Highplex FLHALO
Ablation of the endoplasmic reticulum stress kinase PERK induces paraptosis and type I interferon to promote anti-tumor T cell responsesMandula J, Chang S, Mohamed E, Jimenez R, Sierra-Mondragon R, Chang D, Obermayer A, Moran-Segura C, Das S, Vazquez-Martinez J, Prieto K, Chen A, Smalley K, Czerniecki B, Forsyth P, Koya R, Ruffell B, Cubillos-Ruiz J, Munn D, Shaw T, Conejo-Garcia J, Rodriguez P2022Cancer CellImmuno-oncologyHighplex FLHALO
CD103 and periplakin are potential biomarkers for response of metastatic melanoma to pembrolizumabEdmonds N, Flores S, Mahmutovic A, Young S, Mauldin I, Slingluff C2022Melanoma ResearchImmuno-oncologyHighplex FLHALO
High endothelial venules associated with better prognosis in esophageal squamous cell carcinomaLi H, Tang L, Han X, Zhong L, Gao W, Chen Y, Huang J, Wen Z2022Annals of Diagnostic PathologyOncologyObject Colocalization, Spatial Analysis, Highplex FLHALO
Evolutionary trajectory of receptor binding specificity and promiscuity of the spike protein of SARS-CoV-2Planchais C, Reyes-Ruiz A, Lacombe R, Zarantonello A, Lecerf M, Revel M, Roumenina L, Atanasov B, Mouquet H, Dimitrov J2022Protein ScienceInfectious DiseaseArea Quantification, Highplex FLHALO
Novel post-acquisition image processing to attenuate red blood cell autofluorescence for quantitative image analysisBouffard N, Lee K, DeLance N, Clason T, Chatterjee N, Taatjes D2022Histochemistry and Cell BiologyOtherHighplex FLHALO
Angiogenesis modulation-mediated inhibitory effects of tacrolimus on hypertrophic scar formationShen Y, Jin R, Liang X, Deng Z, He J, Ding Y, Ding F, Lu L, Liu F, Yang J2022Microvascular ResearchDermatologyHighplex FLHALO
Chronic rhinosinusitis patients display an aberrant immune cell localisation with enhanced S. aureus biofilm metabolic activity and biomassShaghayegh G, Cooksley C, Bouras G, Panchatcharam B, Idrizi R, Jana M, Ellis S, Psaltis A, Wormald P, Vreugde S2022Journal of Allergy and Clinical ImmunologyImmunology, Infectious DiseaseClassifier, Spatial Analysis, Highplex FLHALO
Low CD8 T Cell Counts Predict Benefit from Hypoxia-Modifying Therapy in Muscle-Invasive Bladder CancerSmith V, Mukherjee D, Tsakiroglou A, Baker A, Mistry H, Choudhury A, Hoskin P, Illidge T, West C2022CancersImmuno-oncologyHighplex FL, TMAHALO
NUC-7738 regulates _-catenin signalling resulting in reduced proliferation and self-renewal of AML cellsShahid A, Um I, Elshani M, Zhang Y, Harrison D2022PLOS OneOncologyHighplex FLHALO, HALO AI
Innate immune receptor C5aR1 regulates cancer cell fate and can be targeted to improve radiotherapy in tumours with immunosuppressive microenvironmentsBeach C, MacLean D, Majorova D, Melemenidis S, Nambiar D, Kim R, Valbuena G, Guglietta S, Krieg C, Darvish D, Suwa T, Eason A, Domingo E, Moon E, Jiang D, Jiang Y, Koong A, Woodruff T, Graves E, Maughan T, Buczacki S, Stucki M, Le Q-T, Leedham S, Giaccia A, Olcina M2023bioRxivImmuno-oncologyClassifier, Area Quantification, Highplex FLHALO
Single-cell RNA sequencing identifies a population of human liver-type ILC1sKramer B, Nalin A, Ma F, Eickoff S, Lutz P, Leonardelli S, Foeser F, Finnemann C, Hack G, Raabe J, ToVinh M, Ahmad S, Hoffmeister C, Kaiser K, Manekeller S, Branchi V, Bald T, Holzel M, Huneburg R, Nischalke H, Semaan A, Langhans B, Kaczmarek D, Benner B, Lordo M, Kowalski J, Gerhardt A, Timm J, Toma M, Mohr R, Turler A, Charpentier A, van Bremen T, Felmann G, Sattler A, Kotsch K, Abdallah A, Strassburg C, Spengler U, Carson W, Mundy-Bosse B, Pellegrini M, O'Sullivan T, Greud A, Nattermann J2023Cell ReportsImmunologyHighplex FLHALO
Tertiary lymphoid structures in desmoplastic melanoma have increased lymphocyte density, lymphocyte proliferation, and immune cross talk with tumor when compared to non-desmoplastic melanomasEdmonds N, Gradecki S, Katyal P, Lynch K, Stowman A, Gru A, Engelhard V, Slingluff Jr. C, Mauldin I2023OncoImmunologyImmuno-oncologyHighplex FLHALO
Autologous T cell therapy for MAGE-A4+ solid cancers in HLA-A*02+ patients: a phase 1 trialHong D, Van Tine B, Biswas S, McAlpine C, Johnson M, Olszanski A, Clarke J, Araujo D, Blumenschein G, Kebriaei P, Lin Q, Tipping A, Sanderson J, Wang R, Trivedi T, Annareddy T, Bai J, Rafail S, Sun A, Fernandes L, Navenot J-M, Bushman F, Everett J, Karadeniz D, Broad R, Isabelle M, Naidoo R, Bath N, Betts G, Wolchinsky Z, Batrakou D, Van Winkle E, Elefant E, Ghobadi A, Cashen A, Grand'Maison A, McCarthy P, Fracasso P, Norry E, Williams D, Druta M, Liebner D, Odunsi K, Butler M2023Nature MedicineImmuno-oncologyISH/FISH, Spatial Analysis, Highplex FLHALO
A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migrationHwang P, Mathur J, Cao Y, Almeida J, Ye J, Morikis V, Cornish D, Clarke M, Stewart S, Pathak A, Longmore G2023Developmental CellOncologyHighplex FL, RegistrationHALO
Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methodsBayer F, Bejarano D, Bartacchi G, Doffin A-C, Gobbini E, Hubert M, Li L, Meiser P, Pedde A-M, Posch W, Rupp L, Schlitzer A, Schmitz M, Schraml B, Uderhardt S, Balladeau-Guilemond J, Wilflingseder D, Zaderer V, Bottcher J2023European Journal of ImmunologyOtherHighplex FLHALO
Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal‚Ñ¢-TSA multiplex immunohistochemistryQuigley L, Pang L, Tavancheh E, Ernst M, Behren A, Huynh J, Da Gama Duarte J2023STAR ProtocolsOtherHighplex FLHALO
Standardized pathology screening of mature Tertiary Lymphoid Structures in cancersVanhersecke L, Bougouin A, Crombe A, Brunet M, Sofeu C, Parrens M, Pierron H, Bonhomme B, Lembege N, Rey C, Velasco V, Soubeyran I, Begueret H, Bessede A, Bellera C, Scoazec J-Y, Italiano A, Fridman C, Fridman W, Le Loarer F2023Laboratory InvestigationOtherHighplex FL, RegistrationHALO
Effects of electroacupuncture on the kisspeptin-gonadotropin-releasing hormone (GnRH) /luteinizing hormone (LH) neural circuit abnormalities and androgen receptor expression of kisspeptin/neurokinin B/dynorphin neurons in PCOS rats.Xu G, Zhao X, Li Z, Hu J, Li X, Li J, Chen Y2023Journal of Ovarian ResearchOtherHighplex FLHALO
Fucosylation of HLA-DRB1 regulates CD4+ T cell-mediated anti-melanoma immunity and enhances immunotherapy efficacyLester D, Burton C, Gardner A, Innamarato P, Kodumudi K, Liu Q, Adhikari E, Ming Q, Williamson D, Frederick D, Sharova T, White M, Markowitz J, Cao B, Nguyen J, Johnson J, Beatty M, Mockabee-Macias A, Mercurio M, Watson G, Chen P-L, McCarthy S, MoranSegura C, Messina J, Thomas K, Darville L, Izumi V, Koomen J, Pilon-Thomas S, Ruffell B, Luca V, Haltiwanger R, Wang X, Wargo J, Boland G, Lau E2023Nature CancerImmuno-oncologyHighplex FL, RegistrationHALO
Inhibition of indoleamine dioxygenase leads to better control of tuberculosis adjunctive to chemotherapySingh B, Moodley C, Singh D, Escobedo R, Sharan R, Arora G, Ganatra S, Shivanna V, Gonzalez O, Hall-Ursone S, Dick Jr. E, Kaushal D, Alvarez X, Mehra S2023JCI InsightInfectious Disease, FibrosisArea Quantification, Highplex FLHALO
Combined KRASG12C and SOS1 inhibition enhances and extends the anti-tumor response in KRASG12C-driven cancers by addressing intrinsic and acquired resistanceThatikonda V, Lu H, Jurado S, Kostyrko K, Bristow C, Bosch K, Feng N, Gao S, Gerlach D, Gmachi M, Lieb S, Jeschko A, Machado A, Marszalek E, Mahendra M, Jaeger P, Sorokin A, Strauss S, Trapani F, Kopetz S, Vellano C, Petronczki M, Kraut N, Heffernan T, Marszalek J, Pearson M, Waizenegger I, Hofmann M2023bioRxivOncologyISH/FISH, Multiplex IHC, Highplex FL, Figure MakerHALO
Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trialTian J, Chen J, Chao S, Pelka K, Giannakis M, Hess J, Burke K, Jorgji V, Sindurakar P, Braverman J, Mehta A, Oka T, Huang M, Lieb D, Spurrell M, Allen J, Abrams T, Clark J, Enzinger A, Enzinger P, Klempner S, McCleary N, Meyerhardt J, Ryan D, Yurgelun M, Kanter K, Van Seventer E, Baiev I, Chi G, Jarnagin J, Bradford W, Wong E, Michel A, Fetter I, Siravegna G, Gemma A, Sharpe A, Demehri S, Leary R, Campbell C, Yilmaz Om Getz G, Parikh A, Hacohen N, Corcoran R2023Nature MedicineOncologyHighplex FLHALO
Gold Nanoparticles for Monitoring of Mesenchymal Stem Cell–Loaded Bioresorbable Polymeric Wraps for Arteriovenous FistulasBarcena A, Perez J, Damasco J, Bernardino M, San Valentin E, Klusman C, Martin B, Cortes A, Canlas G, Heralde III, F, Avritscher R, Fowlkes N, Bouchard R, Cheng J, Huang S, Melancon M2023bioRxivBiomaterialsHighplex FLHALO
Discovery of NRG1-VII: a novel myeloid-derived class of NRG1 isoformsBerrocal-Rubio M, Pawer Y, Dinevska M, De Paoli-Iseppi R, Widodo S, Rajab N, De Nardo W, Hallab J, Li A, Mantamadiotis T, Clark M, Wells C2023bioRxivImmunologyHighplex FLHALO
Single-cell spatial immune landscapes of primary and metastatic brain tumoursKarimi E, Yu M, Maritan S, Perus L, Rezanejad M, Sorin M, Kankner M, Fallah P, Doré S, Zuo D, Fiset B, Kloosterman D, Ramsay L, Wei Y, Lam S, Alsajjan R, Watson I, Urgoiti G, Park M, Randsma D, Senger D, Chan J, Akkari L, Petrecca K, Guiot M-C, Siegel P, Quail D, Walsh L2023NatureImmuno-oncologyHighplex FLHALO
Multiplex immune profiling reveals the role of serum immune proteomics in predicting response to preoperative chemotherapy of gastric cancerTang Z, Gu Y, Shi Z, Min L, Zhang Z, Zhou P, Luo R, Wang Ym, Cui Y, Sun Y, Wang X2023Cell Reports MedicineImmuno-oncologyHighplex FLHALO
Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analysesHirz T, Mei S, Sarkar H, Kfoury Y, Wu S, Verhoeven B, Subtelny A, Zlatev D, Wszolek M, Salari K, Murray E, Chen F, Macosko E, Wu C, Scadden D, Dahl D, Baryawno N, Saylor P, Kharchenko P, Sykes D2023Nature CommunicationsImmuno-oncologyHighplex FL, Figure Maker, HALO AIHALO
Impact of aging on the immunological and microbial landscape of the lung during non-tuberculous mycobacterial infectionCinco I, Rhoades N, Napier E, Davies M, Allison D, Kohama S, Bermudez L, Winthrop K, Fuss C, Spindel E, Messaoudi I2023bioRxivInfectious DiseaseHighplex FLHALO
Effects of KMT2D mutation and its exon 39 mutation on the immune microenvironment and drug sensitivity in colorectal adenocarcinomaLiu C, Jin Y, Zhang H, Yan J, Guo Y, Bao X, Zhao P2023HeliyonOncologyHighplex FLHALO
NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasisIlter D, Drapela S, Schild T, Ward N, Adhikari E, Low V, Asara J, Oskarsson T, Lau E, DeNicola G, McReynolds M, Gomes A2023Redox BiologyOncology, MetabolismClassifier, Highplex FL, TMAHALO
Spatial transcriptomics analysis of zone-dependent hepatic ischemia-reperfusion injury murine modelXin J, Yang T, Wu X, Wu Y, Liu Y, Liu X, Jiang M, Gao W2023Communications BiologyGastroenterologyHighplex FLHALO
Coordinated activation of c-Src and FOXM1 drives tumor cell proliferation and breast cancer progressionNandi I, Smith H, Sanguin-Gendreau V, Ji L, Pacis A, Papavasiliou V, Zuo D, Nam S, Attala S, Kim S, Lusson S, Kuasne H, Fortier A, Savage P, Ramirez C, Park M, Katzenellenbogen J, Katzenellenbogen B, Muller W2023Journal of Clinical InvestigationOncologyHighplex FLHALO
Oncogenic KrasG12D specific non-covalent inhibitor reprograms tumor microenvironment to prevent and reverse early pre-neoplastic pancreatic lesions and in combination with immunotherapy regresses advanced PDAC in a CD8+ T cells dependent mannerMahadevan K, McAndrews K, LeBlue V, Yang S, Lyu H, Li B, Sockwell A, Kirtley M, Morse S, Diaz B, Kim M, Feng N, Lopez A, Guerrero P, Sugimoto H, Arian K, Ying H, Barekatain Y, Kelly P, Maitra A, Heffernan T, Kalluri R2023bioRxivOncologyFISH, Highplex FLHALO
PD-1+CD8+ T Cells Proximal to PD-L1+CD68+ Macrophages Are Associated with Poor Prognosis in Pancreatic Ductal Adenocarcinoma PatientsYang X, Wang G, Song Y, Zhuang T, Li Y, Xie Y, Fei X, Zhao Y, Xu D, Hu Y2023CancersOncology, Immuno-oncologyClassifier, Spatial Analysis, Highplex FL, Registration, TMAHALO
Donor Batf3 inhibits murine lung allograft rejection and airway fibrosisWatanabe T, Lam C, Oliver J, Oishi H, Teskey G, Beber S, Boonstra K, Mauricio J, Buhari H, Joe B, Guan Z, Horie M, Keshavjee S, Martinu T, Juvet S2023Mucosal ImmunityOther, FibrosisHighplex FLHALO
Loss of Hepatic Leucine-Rich Repeat-Containing G-Protein Coupled Receptors 4 and 5 Promotes Nonalcoholic Fatty Liver DiseaseSaponara E, Penno C, Orsini V, Wang Z, Fischer A, Aebi A, Matadamas-Guzman M, Brun V, Fischer B, Brousseau M, O'Donnell P, Turner J, Meyer A, Bollepalli L, d'Ario G, Roma G, Carbone W, Annunziato S, Obrecht M, Beckmann N, Saravanan C, Osmont A, Tropberger P, Richards S, Genoud C, Ley S, Ksiazek I, Nigsch F, Terracciano L, Schadt H, Bouwmeester T, Tchorz J, Ruffner H2023The American Journal of PathologyGastroenterologyHighplex FLHALO
Single-cell analysis reveals changes in BCG vaccine-injected mice modeling tuberculous meningitis brain infectionZhang X, Zhao Z, Wu Q, Wang L, Li L, Wang M, Ren Y, Pan L, Tang H, Li F2023Cell ReportsInfectious DiseaseMultiplex IHC, Highplex FLHALO
Spatial multi-omics revealed the impact of tumor ecosystem heterogeneity on immunotherapy efficacy in patients with advanced non-small cell lung cancer treated with bispecific antibody Song X, Xiong A, Wu F, Li X, Wang J, Jiang T, Chen P, Zhang X, Zhao Z, Liu H, Cheng L, Zhao C, Wang Z, Pan C, Cui X, Xu T, Luo H, Zhou C2023Journal for ImmunoTherapy of CancerImmuno-oncologyClassifier, Highplex FLHALO
Melanotransferrin Functions as a Pro-Oncogenic WNT Agonist: A Yin-Yang Relationship in Melanoma with the WNT Antagonist and Metastasis Suppressor, NDRG1Paluncic J, Cholam M, Lane D, Skoda J, Park K, Chiang S, Bae D, Scolyer R, Afroz R, Babu G, Wilmott J, Loh K, Jansson P, Dharmasiviam M, Huang M, Zhao X, Kovacevic Z, Richardson D2023bioRxivOncologyHighplex FLHALO
Co-enrichment of CD8-positive T cells and macrophages is associated with clinical benefit of tislelizumab in solid tumorsYe D, Desai J, Shi J, Liu S, Shen W, Liu T, Shi Y, Wang D, Liang L, Yang S, Ma X, Jin W, Zhang P, Huang R, Shen Z, Zhang Y, Wu Y2023Biomarker ResearchImmuno-oncologySpatial Analysis, Highplex FLHALO
Single-cell profiling reveals pathogenic role and differentiation trajectory of granzyme K+ CD8+T cells in primary Sjögren’s syndromeXu T, Zhu H, Ma J, Li X, Luo P, Li Y, Lian Z, Gao C2023JCI InsightImmunologyHighplex FLHALO
SARS-CoV-2 infection induces DNA damage, through CHK1 degradation and impaired 53BP1 recruitment, and cellular senescenceGioia U, Tavella S, Martinez-Orellana P, Cicio G, Colliva A, Ceccon M, Cabrini M, Henriques A, Fumagalli V, Paldino A, Presot E, Rajasekharan S, Iacomino N, Pisati F, Matti V, Sepe S, Conte M, Barozzi S, Lavagnino Z, Carletti T, Volpe M, Cavalcante P, Iannacone M, Rampazzo C, Bussani R, Tripodo C, Zacchigna S, Marcello A, di Fagagna F2023Nature Cell BiologyInfectious DiseaseISH/FISH, Highplex FLHALO
TIPE2 Inhibits MGD Inflammation by Regulating Macrophage PolarizationZhao S, Shen Y, Wu S, Shao Y, Shi R, Yan Y, Zhao H2023Journal of Personalized MedicineImmunologyArea Quantification, Highplex FLHALO
Loss of Peroxiredoxin IV Protects Mice from Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Cancer DevelopmentThapa P, Jiang H, Ding N, Hao Y, Alshahrani A, Lee E, Fujii J, Wei Q2023AntioxidantsOncologyMultiplex IHC, Highplex FLHALO
Bi-allelic mutation in SEC16B alters collagen trafficking and increases ER stressEl-Gazzar A, Voraberger B, Rauch F, Mairhofer M, Schmidt K, Guillemyn B, Mitulovic G, Reiterer V, Haun M, Mayr M, Mayr J, Kimeswenger S, Drews O, Saraff V, Shaw N, Fratzl-Zelman N, Symoens S, Farhan H, Hogler W2023EMBO Molecular MedicineOtherArea Quantification, Highplex FLHALO
Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisSu X, Brassard A, Bartolomucci A, Dhoparee-Dooma I, Qiu Q, Tsering T, Rohanizadeh R, Koufos O, Giannias B, Bourdeau F, Feng L, Leo S, Sangwan V, Tankel J, Quail D, Spicer J, Burnier J, Bailey S, Ferri L, Cools-Lartigue J2023bioRxivImmuno-oncologyArea Quantification, Highplex FLHALO
Chronic immune activation and gut barrier dysfunction is associated with neuroinflammation in ART-suppressed SIV+ rhesus macaquesByrnes S, Busman-Sahay K, Angelovich T, Younger S, Taylor-Brill S, Nekorchuk M, Bondoc S, Dannay R, Terry M, Cochrane C, Jenkins T, Roche M, Deleage C, Bosinger S, Paiardini M, Brew B, Estes J, Churchill M2023PLOS PathogensInfectious DiseaseArea Quantification, Object Colocalization, ISH, Highplex FLHALO
Development of a deep pathomics score for predicting hepatocellular carcinoma recurrence after liver transplantationQu W, Tian M, Lu G, Zhou Y, Liu W, Tang Z, Yao Z, Huang R, Zhu G, Jiang X, Tao C, Fang Y, Gao J, Wu X, Chen J, Zhao Q, Yang R, Chu T, Zhou J, Fan J, Yu J, Shi Y2023Hepatology InternationalOncologySpatial Analysis, Highplex FLHALO
Early Identication of High-Risk Patients with HCC Relapse: A Spatial Immune Scoring System Enabled by Articial IntelligenceSun C, Jia G, He P, Goh D, Li F, Wee F, Sun M, Dai T, Lim J, Hao S, Liu L, Yeong J, Tian Z2023Research SquareOncologyHighplex FLHALO
Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancerShakfa N, Li D, Conseil G, Lightbody E, Wilson-Sanchez J, Hamade A, Chenard S, Jawa N, Laight B, Afriyie-Asante A, Tyryshkin K, Koebel M, and Koti M2023Journal for ImmunoTherapy of CancerOncologyHighplex FLHALO, HALO Link
Prognostic value of tumor immune microenvironment factors in patients with stage I lung adenocarcinomaXue Q, Wang Y, Zheng Q, Chen L, Lin Y, Jin Y, Shen X, Li Y2023American Journal of Cancer ResearchOncologyClassifier, Multiplex IHC, Highplex FLHALO
Immune Checkpoint Neuropilins as Novel Biomarkers and Therapeutic Targets for Pancreatic CancerHe L, Zhang X, Lao M, Zhang H, Yang H, Bai X2023CancersImmuno-oncologyHighplex FLHALO
Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infectionPremadasa L, Lee E, McDew-White M, Alvarez X, Jayakumar S, Ling B, Okeoma C, Byrareddy S, Kulkarni S, and Mohan M2023JCI InsightInfectious DiseaseArea Quantification, Highplex FLHALO
Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancerWang F, Long J, Li L, Wu Z, Da T, Wang X, Huang C, Jiang Y, Yao X, Ma H, Lian Z, Zhao Z, Cao J2023Science AdvancesOncologyMultiplex IHC, Spatial Analysis, Highplex FLHALO
High tumor mutational burden predicts favorable response to anti-PD-(L)1 therapy in patients with solid tumor: a real-world pan-tumor analysisJung J, Heo Y, Park S2023Journal for ImmunoTherapy of CancerImmuno-oncologySpatial Analysis, Highplex FLHALO
Transcriptional-translational conflict is a barrier to cellular transformation and cancer progressionJana S, Brahma S, Arora S, Wladyka C, Hoang P, Blinka S, Hough R, Horn J, Liu Y, Wang L, Depeille P, Smith E, Montgomery R, Lee J, Haffner M, Vakar-Lopez F, Grivas P, Wright J, Lam H, Black P, Roose J, Ryazanov A, Subramaniam A, Henikoff S, and Hsieh A2023Cancer CellOncologyMultiplex IHC, Highplex FLHALO
Geospatial characterization of immune cell distributions and dynamics across the microenvironment in clear cell renal cell carcinomaChakiryan N, Kim Y, Berglund A, Chang A, Kimmel G, Hajiran A, Nguyen J, Moran-Segura C, Saeed-Vafa D, Katende E, Lopez-Blanco N, Chahoud J, Rappold P, Spiess P, Fournier M, Jeong D, Wang L, Teer J, Dhillon J, Kuo F, Hakimi A, Altrock P, Mulé J, Manley B2023Journal for ImmunoTherapy of CancerImmuno-oncologyHighplex FLHALO
Adrenal cortex size and homeostasis are regulated by gonadal hormones via androgen receptor/β-catenin signaling crosstalkLyraki R, Grabek A, Tison A, Weerasinghe-Arachchige L, Peitzsch M, Bechman N, Youssef S, de Bruin A, Bakker E, Claessens F, Chaboissier M, Schedl A2023Disease Models & MechanismsOtherFISH, Highplex FLHALO
Multi-organ landscape of therapy-resistant melanomaLiu S, Dharanipragada P, Lomeli S, Wang Y, Zhang X, Yang Z, Lim R, Dumitras C, Scumpia P, Dubinett S, Moriceau G, Johnson D, Moschos S, Lo R2023Nature MedicineOncology, Immuno-oncologyHighplex FLHALO
Somatic mutation profiling, tumor-infiltrating leukocytes, tertiary lymphoid structures and PD-L1 protein expression in HER2-amplified colorectal cancerLiu X, Kou Z, Zhang H, Dong J, Zhang J, Peng Y, Ma S, Liang L, Meng X, Zhou Y, Yang J2023PeerJImmuno-oncologyHighplex FLHALO
Signal-transducing adaptor protein 1 (STAP1) in microglia promotes the malignant progression of gliomaYang X, Ji C, Qi Y, Huang J, Hu L, Zhou Y, Zou L, Xia Y, Tan F, Yao Y, Chen D2023Research SquareOncologyHighplex FLHALO
Intracranial injection of natural killer cells engineered with a HER2-targeted chimeric antigen receptor in patients with recurrent glioblastoma Burger M, Forster M, Romanski A, Straßheimer F, Macas J, Zeiner P, Steidl E, Herkt S, Weber K, Schupp J, Lun J, Strecker M, Wlotzka K, Cakmak P, Opitz C, George R, Mildenberger I, Nowakowska P, Zhang C, Röder J, Müller E, Ihrig K, Langen K, Rieger M, Herrmann E, Bonig H, Harter P, Reiss Y, Hattingen E, Rödel F, Plate K, Tonn T, Senft C, Steinbach J, Wels W2023Neuro-OncologyImmuno-oncologyHighplex FLHALO
Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosisConroy L, Clarke H, Allison D, Valenca S, Sun Q, Hawkinson T, Young L, Ferreira J, Hammonds A, Dunne J, McDonald R, Absher K, Dong B, Bruntz R, Markussen K, Juras J, Alilain W, Liu J, Gentry M, Angel P, Waters C, Sun R2023Nature CommunicationsFibrosisMultiplex IHC, Highplex FLHALO
Spatial analysis with SPIAT and spaSim to characterize and simulate tissue microenvironmentsFeng Y, Yang T, Zhu J, Li M, Doyle M, Ozcoban V, Bass G, Pizzolla A, Cain L, Weng S, Pasam A, Kocovski N, Huang Y, Keam S, Speed T, Neeson P, Pearson R, Sandhu S, Goode D, Trigos A2023Nature CommunicationsOtherHighplex FLHALO
High expression ITGA2 affects the expression of MET,PD-L1, CD4 and CD8 with the immune microenvironment in pancreatic cancer patientsJin L, Duan Y, Li Z, Hu J, Shi H, Su Z, Li Z, Du B, Chen Y, Tan Y2023Research SquareOncologyHighplex FLHALO
Sequential pembrolizumab cooperates with platinum/5FU to remodel the tumor immune microenvironment in advanced gastric cancer: A phase II chemoimmunotherapy trialKlempner S, Lee J, Mehta A, An M, Min B, Heo Y, Parikh M, Bi L, Cristescu R, Lee H, Kim T, Lee S, Moon J, Park R, Strickland M, Park W, Kang W, Kim K, Kim S2023Research SquareImmuno-oncologyClassifier, Highplex FLHALO
An Fc-muted bispecific antibody targeting PD-L1 and 4-1BB induces antitumor immune activity in colorectal cancer without systemic toxicityCheng L, Zhu M, Gao Y, Liu W, Yin W, Zhou P, Zhu Z, Niu L, Zeng X, Zhang D, Fang Q, Wang F, Zhao Q, Zhang Y, Shen G2023Cellular and Molecular Biology LettersImmuno-oncologyMultiplex IHC, Highplex FLHALO
Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsisZhang S, Xu Q, Shi L, Li S, Wang W, Wang Q, Lu L, Xiao H, Wang J, Li F, Liang Y, Gong S, Peng H, Zhang Z, Tang G2023Signal Transduction and Targeted TherapyImmunologyHighplex FLHALO
Activation of IGFBP4 via unconventional mechanism of miRNA attenuates metastasis of intrahepatic cholangiocarcinomaTao L, Wang Y, Shen Z, Cai J, Zheng J, Xia S, Lin Z, Wan Z, Qi H, Jin R, Wang L, Xu J, Liang X2023Hepatology InternationalOncologyHighplex FLHALO
Characterization of intratumoral tertiary lymphoid structures in pancreatic ductal adenocarcinoma: cellular properties and prognostic significanceZou X, Lin X, Cheng H, Chen Y, Wang R, Ma M, Liu Y, Dai Z, Tasiheng Y, Yan Y, Hou Q, Ding F, Chen H, Yu X, Wang X, Liu C2023Journal for ImmunoTherapy of CancerImmuno-oncologyHighplex FLHALO, HALO AI
Trackable Intratumor Microdosing and Spatial Profiling Provide Early Insights into Activity of Investigational Agents in the Intact Tumor MicroenvironmentDerry J, Burns C, Frazier J, Beirne E, Grenley M, DuFort C, Killingbeck E, Leon M, Williams C, Gregory M, Houlton J, Clayburgh D, Swiecicki P, Huszar D, Berger A, Klinghoffer R2023Cellular and Molecular Biology LettersOncologyHighplex FLHALO, HALO AI
A cellular and molecular spatial atlas of dystrophic muscleStec M, Su Q, Adler C, Zhang L, Golann D, Khan N, Panagis L, Villalta S, Ni M, Wei Y, Walls J, Murphy A, Yancopoulos G, Atwal G, Kleiner S, Halasz G, Sleeman M2023Proceedings of the National Academy of SciencesMyologyISH/FISH, Highplex FLHALO
Stem cell-like T cell depletion in the recurrent head and neck cancer immune microenvironmentChen L, Lee N, Sarkar R, Katabi N, Li Y, Morris L, Wong R, Sherman E, Reis-Filho J, Hollmann T, Riaz N2023OncoImmunologyImmuno-oncologySpatial Analysis, Highplex FLHALO
TSLP/dendritic cell axis promotes CD4+ T cell tolerance to the gut microbiomeMesserschmidt J, Azin M, Dempsey K, Demehri S2023JCI InsightImmunologyHighplex FLHALO
Poor outcome in postpartum breast cancer patients is associated with distinct molecular and immunological featuresLefrère H, Moore K, Floris G, Sanders J, Seignette I, Bismeijer T, Peters D, Broeks A, Hooijberg E, Van Calsteren K, Neven P, Warner E, Peccatori F, Loibl S, Maggen C, Han S, Jerzak K, Annibali D, Lambrechts D, de Visser K, Wessels L, Lenaerts L, Amant F2023Clinical Cancer ResearchOncologyClassifier, Highplex FLHALO, HALO AI
Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosisBurkard T, Herrero San Juan M, Dreis C, Kiprina A, Namgaladze D, Siebenbrodt K, Luger S, Foerch C, Pfeilschifter J, Weigert A, Radeke H2023Clinical and Translational MedicineOncology, immunologyClassifier, Spatial Analysis, Highplex FL, TMAHALO
An intestinal Th17 subset is associated with inflammation in Crohn’s Disease and activated by adherent-invasive Escherichia coli (AIEC)Paroni M, Leccese G, Ranzani V, Moschetti G, Chiara M, Perillo F, Ferri S, Clemente F, Noviello D, Conforti FS, Ferrero S, Karnani B, Bosotti R, Vasco C, Curti S, Crosti MC, Gruarin P, Rossetti G, Conte MP, Vecchi M, Pagani M, Landini P, Facciotti F, Abrignani S, Caprioli F, Geginat J2023Journal of Crohn's and ColitisImmunologyHighplex FLHALO
Single-cell characterization of pulmonary nodules implicates suppression of immunosurveillance across early stages of lung adenocarcinomaYanagawa J, Tran L, Salehi-Rad R, Lim R, Dumitras C, Fung E, Wallace W, Prosper A, Fishbein G, Shea C, Hong R, Kahangi B, Deng J, Gower A, Liu B, Campbell J, Mazzilli S, Beane J, Kadara H, Lenburg M, Spira A, Aberle D, Krysan K, Dubinett S2023Cancer ResearchImmuno-oncologyHighplex FLHALO
The CD73 is induced by TGF-β1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanomaGiraulo C, Turiello R, Orlando L, Leonardelli S, Landsberg J, Belvedere R, Rolshoven G, Muller C, Holzel M, Morello S2023Biomedicine & PharmacotherapyOncologyHighplex FL, TMAHALO, HALO AI
Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for PathologistsHarms P, Frankel T, Moutafi M, Rao A, Rimm D, Taube J, Thomas D, Chang M, Pantanowitz L2023Modern PathologyReviewSpatial Analysis, Highplex FLHALO
Low molecular weight heparin synergistically enhances the efficacy of adoptive and anti-PD-1-based immunotherapy by increasing lymphocyte infiltration in colorectal cancer Quan Y, He J, Zou Q, Zhang L, Sun Q, Huang H, Li W, Xie K, Wie F2023Journal for ImmunoTherapy of CancerImmuno-oncologyClassifier, Multiplex IHC, Highplex FLHALO
Immune checkpoint blockade induces distinct alterations in the microenvironments of primary and metastatic brain tumorsSun L, Kienzler JC, Reynoso JG, Lee A, Shiuan E, Li S, Kim J, Ding L, Monteleone AJ, Owens GC, Phillips JJ, Everson RG, Nathanson D, Cloughesy TF, Li G, Liau LM, Hugo W, Kim W, Prins RM2023Journal of Clinical InvestigationImmuno-oncologyHighplex FLHALO
Integrative multi-omics reveals two biologically distinct groups of pilocytic astrocytomaPicard D, Felsberg J, Langini M, Stachura P, Qin N, Macas J, Reiss Y, Bartl J, Selt F, Sigaud R, Meyer FD, Stefanski A, Stühler K, Roque L, Roque R, Pandyra AA, Brozou T, Knobbe-Thomsen C, Plate KH, Roesch A, Milde T, Reifenberger G, Leprivier G, Faria CC, Remke M2023Acta Neuropathologica OncologyHighplex FLHALO
Integration of peripheral blood- and tissue-based biomarkers of response to immune checkpoint blockade in urothelial carcinomaVanguri RS, Smithy JW, Li Y, Zhuang M, Maher CA, Aleynick N, Peng X, Al-Ahmadie H, Funt SA, Rosenberg JE, Iyer G, Bajorin D, Mathews JC, Nadeem S, Panageas KS, Shen R, Callahan MK, Hollmann TJ2023Journal of PathologyImmuno-oncologyHighplex FLHALO, HALO AI
A pan-cancer single-cell panorama of human natural killer cellsTang F, Li J, Qi L, Liu D, Bo Y, Qin S, Miao Y, Yu K, Hou W, Li J, Peng J, Tian Z, Zhu L, Peng H, Wang D, Zhang Z2023CellImmuno-oncologyHighplex FLHALO
Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammationO'Brien VP, Kang Y, Shenoy MK, Finak G, Young WC, Dubrulle J, Koch L, Rodriguez Martinez AE, Williams J, Donato E, Batra SK, Yeung CCS, Grady WM, Koch MA, Gottardo R, Salama NR2023Cancer Research CommunicationsOncology, Infectious DiseaseHighplex FLHALO
Macrophage reprogramming—rather than depletion —is efficacious in a specific subset of colorectal tumor modelsMohamed N, Amirkhah R, Lavaud X, Gilroy K, Bartolini R, Mulholland E, Garg A, Pennel K, Jackstadt R, Ridgway R, Nixon C, Hatthakarnku P, Campbell A, Leedham S, Edwards J, Dunne P, Barry S, Graham G, Sansom O2023Research SquareImmuno-oncologyMultiplex IHC, Highplex FLHALO
Proteogenomic analysis of chemo-refractory highgrade serous ovarian cancerChowdhury S, Kennedy JJ, Ivey RG, Murillo OD, Hosseini N, Song X, Petralia F, Calinawan A, Savage SR, Berry AB, Reva B, Ozbek U, Krek A, Ma W, Leprevost FdV, Ji J, Yoo S, Lin C, Voytovich UJ, Huang Y, Lee SH, Bergan L, Lorentzen TD, Mesri M, Rodriguez H, Hoofnagle AN, Herbert ZT, Nesvizhskii AI, Zhang B, Whiteaker JR, Fenyo D, McKerrow W, Wang J, Schürer SC, Stathias V, Chen XS, Barcellos-Hoff MH, Starr TK, Winterhoff BJ, Nelson AC, Mok SC, Kaufmann SH, Drescher C, Cieslik M, Wang P, Birrer MJ, Paulovich AG2023CellOncologyHighplex FLHALO, HALO Link
Early Detection of Genotoxic Hepatocarcinogens in Rats Using γH2AX and Ki-67: Prediction by Machine LearningMichiba A, Gi M, Yokohira M, Sakurai E, Teramoto A, Kiriyama Y, Yamada S, Wanibuchi H, Tsukamoto T2023Toxicological SciencesOncologyMultiplex IHC, Highplex FLHALO
Regulation of antigen-specific T cell infiltration and spatial architecture in multiple myeloma and premalignancyRobinson MH, Villa NY, Jaye DL, Nooka AK, Duffy A, McCachren SS, Manalo J, Switchenko JM, Barnes S, Potdar S, Azeem MI, Horvat AA, Parihar VC, Gong J, Liang Y, Smith GH, Gupta VA, Boise LH, Kaufman JL, Hofmeister CC, Joseph NS, Lonial S, Dhodapkar KM, Dhodapkar MV2023Journal of Clinical InvestigationImmuno-oncologyHighplex FLHALO
ATR-binding lncRNA ScaRNA2 promotes cancer resistance through facilitating efficient DNA end resection during homologous recombination repairChen Y, Shen H, Liu T, Cao K, Wan Z, Du Z, Wang H, Yu Y, Ma S, Lu E, Zhang W, Cai J, Gao F, Yang Y2023Journal of Experimental & Clinical Cancer ResearchOncologyHighplex FL, TMAHALO
Combined toxic effects of MWCNTs and ZnO nanoparticle on the liver of common carpGao X, Huang Y, Ren H, Li Y, Chen J, Xu R2023Research SquareToxicologyHighplex FLHALO
GRK2 inhibits Flt-1+ macrophage infiltration and its proangiogenic properties in rheumatoid arthritisYang X, Zhao Y, Wei Q, Zhu X, Wang L, Zhang W, Liu X, Kuai J, Wang F, Wei W2023Acta Pharmacologica Sinica BImmunologyHighplex FLHALO
Perspectives for immunotherapy of EBV-associated GLELC: A relatively “hot” tumor microenvironmentLei Y, Cao P, Zheng X, Wi J, Cheng M, Liu M2023Cancer MedicineOncologyHighplex FLHALO
Remodeling Tumor Immune Microenvironment by Using Polymer-Lipid-Manganese Dioxide Nanoparticles with Radiation Therapy to Boost Immune Response of Castration-Resistant Prostate CancerZetrini AE, Lip H, Abbasi AZ, Alradwan I, Ahmed T, He C, Henderson JT, Rauth AM, Wu XY2023ResearchImmuno-oncology, BiomaterialsHighplex FLHALO
Effects and Mechanisms of the Subtypes of Apolipoprotein E to the Immune State and Prognosis of hepatocellular carcinoma patientsGao B, Zhou P, Wang L, Wang Z, Yi Y, Zhou J, Fan J, Qiu S, Xu Y2023Research SquareImmuno-oncologyMultiplex IHC, Highplex FLHALO
Single-cell RNA-sequencing reveals heterogeneity and intercellular crosstalk in human tuberculosis lungWang, H, Wen, Z, Niu, L, Chen, X, Liu, H, Zhang, S, Xu, J, Zhu, Y, Li, H, Chen, H, Shi, L, Wan, L, Li, L, Li, M, Wong, K, Song, Y2023Journal of InfectionImmunology, Infectious DiseaseSpatial Analysis, Highplex FLHALO
An immune cell atlas reveals the dynamics of human macrophage specification during prenatal developmentWang Z, Wu Z, Wang H, Feng R, Wang G, Li M, Wang S, Chen X, Su Y, Wang J, Zhang W, Bao Y, Lan Z, Song Z, Wang Y, Luo X, Zhao L, Hou A, Tian S, Gao H, Miao W, Liu Y, Wang H, Yin C, Ji Z, Feng M, Liu H, Diao L, Amit I, Chen Y, Zeng Y, Ginhoux F, Wu X, Zhu Y, Li H2023CellImmunologyHighplex FLHALO
The prognostic value of CD39 +CD8 + T cells as a potential surrogate marker of tumor-specific T cells in Asian triple-negative breast cancerMeng J, Tira TJY, Joseph CR, Ye J, Lim JCT, Goh D, Yuezhen X, Lim X, Koh VCY, Wee F, Tay TKY, Chan JV, Ng CCY, Iqbal J, Lau MC, Elaine LH, Chong TH, The BT, Dent RA, Tan PH, Sheng JYP2023Research SquareImmuno-oncologyHighplex FLHALO
Tumor-associated macrophages trigger MAIT cell dysfunction at the HCC invasive marginRuf B, Bruhns M, Babaei S, Kedei N, Ma L, Revsine M, Benmebarek MR, Ma C, Heinrich B, Subramanyam V, Qi J, Wabitsch S, Green BL, Bauer KC, Myojin Y, Greten LT, McCallen JD, Huang P, Trehan R, Wang X, Nur A, Soika DQM, Pouzolles M, Evans CN, Chari R, Kleiner DE, Telford W, Dadkhah K, Ruchinskas A, Stovroff MK, Kang J, Oza K, Ruchirawat M, Kroemer A, Wang XW, Claassen M, Korangy F, Greten TF2023CellImmuno-oncologyClassifier, Spatial Analysis, Highplex FL, Figure MakerHALO
Comprehensive analysis of immune subtypes reveals the prognostic value of cytotoxicity and FAP+ fibroblasts in stomach adenocarcinomaWang X, Hui S, Tan C, Deng Z, Wang X, Weng W, Zhang M, Ni S, Wang L, Huang D, Wang W, Xu M, Sheng W2023Cancer Immunology, ImmunotherapyImmuno-oncologyHighplex FLHALO
Tumor cell HLA class I expression and pathologic response following neoadjuvant immunotherapy for newly diagnosed head and neck cancerRobbins Y, Friedman J, Redman J, Sievers C, Lassoued W, Gulley J, Allen C2023Oral OncologyImmuno-oncologyClassifier, Spatial Analysis, Highplex FLHALO
Anticoagulants Enhance Molecular and Cellular Immunotherapy of Cancer by Improving Tumor Microcirculation Structure and Function and Redistributing Tumor InfiltratesWei F, Su Y, Quan Y, Li X, Zou Q, Zhang L, Li S, Jiang M, Lin G, Liang P, He J, Xie K2023Clinical Cancer ResearchImmuno-oncologyClassifier, Multiplex IHC, Spatial Analysis, Highplex FLHALO
Combination IFNβ and membrane-stable CD40L maximize tumor dendritic cell activation and lymph node trafficking to elicit systemic T-cell immunityZheng H, Yu X, Ibrahim M, Foresman D, Xie M, Johnson J, Boyle T, Ruffell B, Perez B, Antonia S, Ready N, Saltos A, Cantwell M, Beg A2023Cancer Immunology ResearchImmuno-oncologyHighplex FLHALO
Key Residue in the Precursor Region of M Protein Contributes to the Neurovirulence and Neuroinvasiveness of the African Lineage of Zika VirusHe M, Wang H, Yan X, Lou Y, Song G, Li R, Zhu Z, Zhang R, Qin C, Li X2023Journal of VirologyInfectious DiseaseHighplex FLHALO
Apolipoprotein E induces pathogenic senescent-like myeloid cells in prostate cancerBancaro N, Cali B, Troiani M, Elia A, Arzola R, Attanasio G, Lai P, Crespo M, Gurel B, Pereira R, Guo C, Mosole S, Brina D, D'Ambrosio M, Pasquini E, Spataro C, Zagato E, Rinaldi A, Pedotti M, Lascio S, Meani F, Montopoli M, Ferrari M, Gallina A, Varani L, Mestre R, Bolis M, Sommer S, de Bono J, Calcinotto A, Alimonti A2023Cancer CellOncologyClassifier, Highplex FLHALO
Acute Influenza Infection Promotes Lung Tumor Growth by Reprogramming the Tumor MicroenvironmentGarmendia I, Varthaman A, Marmier S, Angrini M, Matchoua I, Darbois-Delahousse A, Josseaume N, Foy P, Roumenina L, Naouar N, Meylan M, Siberil S, Russick J, Joubert P, Leroy K, Damotte D, Mansuet-Lupo A, Wislez M, Alifano M, Menger L, Garcia-Verdugo I, Sallenave J, Lantz O, Petitprez F, Cremer I2023Cancer Immunology ResearchOncology, Infectious DiseaseHighplex FLHALO
Spatial mapping reveals granuloma diversity and histopathological superstructure in human tuberculosisSawyer A, Patrick E, Edwards J, Wilmott J, Fielder T, Yang Q, Barber D, Ernst J, Britton W, Palendira U, Chen X, Feng C2023Journal of Experimental MedicineImmunologyMultiplex IHC, Spatial Analysis, Highplex FLHALO
Measurement of ovarian tumor immune profiles by multiplex immunohistochemistry: implications for epidemiologic studiesHathaway C, Conejo-Garcia J, Fridley B, Rosner B, Saeed-Vafa D, Segura C, Nguyen J, Hecht J, Sasamoto N, Terry K, Tworoger S, Townsend M2023Cancer Epidemiology, Biomarkers & PreventionImmuno-oncologyHighplex FLHALO
New insight on the correlation of immune landscapes with immune markers expression in different risk classification of gastrointestinal stromal tumorsZhang Q, Sun X, Hou Y, Gao X, Shen K, Qin X2023Journal of GastroenterologyImmuno-oncologyHighplex FLHALO
T cell immunotherapies engage neutrophils to eliminate tumor antigen escape variantsHirschhorn D, Budhu S, Kraehenbuehl L, Gigoux M, Schröder D, Chow A, Ricca J, Gasmi B, De Henau O, Mangarin L, Li Y, Hamadene L, Flamar A, Choi H, Cortez C, Liu C, Holland A, Schad S, Schulze I, Warner A, Hollmann T, Arora A, Panageas K, Rizzuto G, Duhen R, Weinberg A, Spencer C, Ng D, He X, Albrengues J, Redmond D, Egeblad M, Wolchok J, Merghoub T2023CellArea Quantification, Highplex FLHALO
Plaque Macrophage-Targeting Nanosystems with Cooperative Co-Regulation of ROS and TRAF6 for Stabilization of Atherosclerotic PlaquesYang Q, Jiang H, Wang Y, Leng X, Wang Y, Tong J, Zhou Y, Mo C, Peng J, Gao H2023Advanced Functional MaterialsBiomaterialsHighplex FLHALO
Functional specialization of short-lived and long-lived macrophage subsets in human tonsilsAlaoui L, Villar J, Leclere R, Le Gallou S, Relouzat F, Michaud H, Tarte K, Teissier N, Favier B, Roussel M, Segura E2023Journal of Experimental MedicineImmunologyHighplex FLHALO
Hepatic proinflammatory myeloid phenotypes are a hallmark of Ebola virus Kikwit pathogenesis in rhesus monkeysTseng A, Carossino M, Gertje H, O'Connell A, Gummuluru S, Kolachalama V, Balasuriya U, Connor J, Bennett R, Liu D, Hensley L, Crossland N2023Veterinary PathologyInfectious DiseaseArea Quantification, Highplex FLHALO
Circulating and intratumoral immune determinants of response to atezolizumab plus bevacizumab in patients with variant histology or sarcomatoid renal cell carcinomaSaliby R, Zarif T, Bakouny Z, Shah V, Xie W, Flippot R, Denize T, Kane M, Madsen K, Ficial M, Hirsch L, Wei X, Steinarter J, Harshman L, Vaishampayan U, Severgnini M, McDermott D, Lee G, Xu W, Van Allen E, McGregor B, Signoretti S, Choueiri T, McKay R, Braun D2023Cancer Immunology ResearchImmuno-oncologyHighplex FLHALO
The tumor immune microenvironment of nasopharyngeal carcinoma after gemcitabine plus cisplatin treatmentLv J, Wei Y, Yin J, Chen Y, Zhou G, Wei C, Liang X, Zhang Y, Zhang C, He S, He Q, Huang Z, Guan J, Shen J, Li X, Li J, Li W, Tang L, Mao Y, Guo R, Sun R, Zheng Y, Zhou W, Xiong K, Wang S, Jin X, Liu N, Li G, Kuang D, Sun Y, Ma J2023Nature MedicineOncologyMultiplex IHC, Highplex FLHALO
Bavituximab decreases immunosuppressive myeloid-derived suppressor cells in newly diagnosed glioblastoma patientsLy K, Richardson L, Liu M, Muzikansky A, Cardona J, Lou K, Beers A, Chang K, Brown J, Ma X, Reardon D, Arrillaga-Romany I, Forst D, Jordan J, Lee E, Dietrich J, Nayak L, Wen P, Chukwueke U, Giobbie-Hurder A, Choi B, Batchelor T, Kalpathy-Cramer J, Curry W, Gerstner E2023Clinical Cancer ResearchImmuno-oncologyHighplex FLHALO
Single-cell dissection of cervical cancer reveals key subsets of the tumor immune microenvironmentCao G, Yue J, Ruan Y, Han Y, Zhi Y, Lu J, Liu M, Xu X, Wang J, Gu Q, Wen X, Gao J, Zhang Q, Kang J, Wang C, Li F2023The EMBO JournalImmuno-oncologyHighplex FLHALO
Molecular determinants of clinical outcomes of pembrolizumab in recurrent ovarian cancer: Exploratory analysis of KEYNOTE-100Ledermann JA, Shapira-Frommer R, Santin AD, Lisyanskaya AS, Pignata S, Vergote I, Raspagliesi F, Sonke GS, Birrer M, Provencher DM, Sehouli J, Colombo N, González-Martín A, Oaknin A, Ottevanger PB, Rudaitis V, Kobie J, Nebozhyn M, Edmondson M, Sun Y, Cristescu R, Jelinic P, Keefe SM, Matulonis UA2023Gynecologic OncologyImmuno-oncologyClassifier, Highplex FLHALO
Classification of the tumor immune microenvironment and associations with outcomes in patients with metastatic melanoma treated with immunotherapiesAdegoke N, Gide T, Mao Y, Quek C, Patrick E, Carlino M, Lo S, Menzies A, da Silva P, Vergara I, Long G, Scolyer R, Wilmott J2023Journal for ImmunoTherapy of CancerImmuno-oncologyClassifier, Highplex FLHALO
The PD-1- and LAG-3-targeting bispecific molecule tebotelimab in solid tumors and hematologic cancers: a phase 1 trialLuke J, Patel R, Blumenschein R, Hamilton E, Chmielowski B, Ulahannan S, Connolly R, Santa-Maria C, Wang J, Bahadur S, Weickhardt A, Asch A, Mallesara G, Clingan P, Dlugosz-Danecka M, Tomaszewska-Kiecana M, Pylypenko H, Hamad N, Kindler H, Sumrow B, Kaminker P, Chen F, Zhang X, Shah K, Smith D, De Costa A, Li J, Li H, Sun J, Moore P2023Nature MedicineImmuno-oncologyHighplex FLHALO
High-plex spatial transcriptomic profiling reveals distinct immune components and the HLA class I/DNMT3A/CD8 modulatory axis in mismatch repair-deficient endometrial cancerGuo J, Tang B, Fu J, Zhu X, Xie W, Wang N, Ding Z, Song Z, Yang Y, Xu G, Xiao X2023Cellular OncologyImmuno-oncologyHighplex FL, Figure MakerHALO
Carcinogen exposure enhances cancer immunogenicity by blocking the development of an immunosuppressive tumor microenvironmentHuang M, Xia Y, Li K, Shao F, Feng Z, Li T, Azin M, Demehri S2023The Journal of Clinical InvestigationImmuno-oncologyHighplex FLHALO
The Expression of the Alpha7 Nicotinic Acetylcholine Receptor and the Effect of Smoking in Curdlan-Administered SKG MiceKim YE, Lee JH, Lee EJ, Kim DH, Jeong MR, Hong S, Lee CK, Yoo B, Youn J, Chang EJ, Kim YG2023BiomedicinesImmunologyHighplex FLHALO
Targeting pancreatic cancer metabolic dependencies through glutamine antagonismEncarnación-Rosado J, Sohn A S W, Biancur D E, Lin E Y, Osorio-Vasquez V, Rodrick T, González-Baerga D, Zhao E, Yokoyama Y, Simeone D M, Jones D R, Parker S J, Wild R, Kimmelman A C2023Nature CancerOncologyHighplex FLHALO, HALO AI

Related HALO Modules

TMA (Tissue Microarray) Add-On

Module to assist in the segmentation of TMA spots for further analysis.

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Tissue Classifier Add-On

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Spatial Analysis

Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.

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