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HALO® Module

Islet Quantification

The Islet Quantification module counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and/or nuclear positivity for up to two stains.

In addition to measuring cytoplasmic positivity, this tool can also be configured to detect positivity within islet nuclei as shown in the second example. Here, proliferating beta cells are identified as dual positive for insulin (red stain) and Ki67 (brown nuclear stain). Insulin positive cells are marked red in the cytoplasm and Ki67 positive cells are marked green in the nucleus.

The module reports islet counts, and islet measurements (diameter & area), islet cell counts (beta cells, positive cells, dual positive cells, and negative cells), and a histogram of islet frequency by diameter ranges.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Islet IHC or FL modules. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Single-Cell RNAseq Reveals That Pancreatic β-Cells From Very Old Male Mice Have a Young Gene SignatureXin Y, Kim J, Okamoto H, Ni M, Wei Y, Adler C, Murphy AJ, Yancopoulos GD, Lin C, Gromada J2016EndocrinologyMetabolismIslet

Related HALO Modules

Multiplex IHC

Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.

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Tissue Classifier Add-On

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Vacuole Quantification

Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.

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Use the arrows above to view additional related modules

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