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HALO® Module

Vacuole Quantification

The Vacuole Quantification module is a multi-purpose module that can be used for quantifying the number and size of white spaces in tissue. Typical applications are lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Vacuole Quantification module. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO ModulesProduct
A suboptimal maternal diet combined with accelerated postnatal growth results in an altered aging profile in the thymus of male rats Tarry-Adkins JL, Aiken CE, Ashmore TJ, Fernandez-Twinn DS, Chen J-H, Ozanne SE2018The FASEB JournalNeuroscience, MetabolismClassifier, Vacuole HALO
Phenotypic characterization of Adig null mice suggests roles for adipogenin in the regulation of fat mass accrual and leptin secretionAlvarez-Guaita A, Patel S, Lim K, Haider A, Dong L, Conway OJ, Ma MKL, Chiarugi D, Saudek V, O'Rahilly S, Savage DB2021Cell ReportsMetabolismVacuoleHALO
Breast adipocyte size associates with ipsilateral invasive breast cancer risk after ductal carcinoma in situAlmekinders MMM, Schaapveld M, Thijssen B, Visser LL, Bismeijer T, Sanders J, Isnaldi E, Hofland I, Mertz M, Wessels LFA, Broeks A, Hooijberg E, Zwart W, Lips EH, Grand Challenge PRECISION Consortium, Desmedt C, Wesseling J2021npj Breast CancerOncologyVacuoleHALO
Allostatic hypermetabolic response in PGC1?/? heterozygote mouse despite mitochondrial defectsRodriguez-Cuenca S, Lelliot C, Campbell M, Peddinti G, Martinuez-UÒa M, Ingvorsen C, Dias A, Relat J, Mora S, Hyˆtyl‰inen T, Zorzano A, Oreöi? M, Bjursell M, Bohlooly-Y M, LindÈn D, Vidal-Puid A2021The FASEB JournalMetabolismVacuoleHALO
AST-120 Treatment Alters the Gut Microbiota Composition and Suppresses Hepatic Triglyceride Levels in Obese MiceHiraga Y, Kubota T, Katoh, M, et al. 2021Endocrine ResearchMetabolismVacuoleHALO
Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesityPetkevicius K, Virtue S, Bidault G, Jenkins B, Cubuk C, Morgantini C, Aouadi M, Dopazo J, Serlie M, Koulman A, Vidal-Puig A2019ElifeClassifier, Vacuole HALO
Evaluation of a PEGylated Fibroblast Growth Factor 21 Variant Using Novel Preclinical Magnetic Resonance Imaging and Magnetic Resonance Elastography in a Mouse Model of Nonalcoholic SteatohepatitisTang H, Li J, Zinker B, Boehm S, Mauer A, Rex-Rabe S, Glaser K, Fronheiser M, Bradstreet T, Nakao Y, Petrone T, Pena A, Villano M, Chow P, Malhi H, Charles E, Hayes W, Ehman R, Du S, Yin M2022Journal of Magnetic Resonance ImagingMetabolismVacuoleHALO
Obesity due to Steroid Receptor Coactivator-1 deficiency is associated with endocrine and metabolic abnormalitiesCacciottolo T, Henning E, Keogh J, Lassen P, Lawler K, Bounds R, Ahmed R, Perdikari A, Mendes de Oliverira E, Smith M, Godfrey E, Johnson E, Hodson L, Clement K, van der Klaauw A, Farooqi I2022The Journal of Clinical Endocrinology & MetabolismMetabolismVacuoleHALO
Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed micePatel S, Haider A, Alvarez-Guaita A, Bidault G, Moustafa J, Guiu-Jurado E, Tadross J, Warner J, Harrison J, Virtue S, Scurria F, Zvetkova I, Bluher M, Small K, O'Rahilly S, Savage D2022Molecular MetabolismMetabolismVacuoleHALO
Defective extracellular matrix remodeling in brown adipose tissue is associated with fibro-inflammation and reduced diet-induced thermogenesisPellegrinelli V, Figueroa-Juárez E, Samuelson I, U-Din M, Rodriguez-Fdez S, Virtue S, Leggat J, Çubuk C, Peirce V, Niemi T, Campbell M, Rodriguez-Cuenca S, Dopazo Blázquez J, Carobbio S, Virtanen K, Vidal-Puig A2023Cell ReportsMetabolismClassifier, Area Quantification, VacuoleHALO
Skeletal phenotype amelioration in Mucopolysaccharidosis VI requires intervention at the earliest stages of postnatal developmentHwang-Wong E, Amar G, Das N, Zhang X, Aaron N, Gale K, Rothman N, Fante M, Baik A, Bhargava A, Fricker A, McAlister M, Rabinowitz J, Lees-Shepard J, Nannuru K, Economides AN, Cygnar KD2023JCI InsightOtherVacuole, Layer ThicknessHALO

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Count pancreatic islets fluorescently-labeled with up to three dyes, quantify number of dye positive cells per islet and area of dye positivity per islet.

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