Vacuole Quantification Module

Quantify the number and size of white spaces in tissue in brightfield images with the HALO® Vacuole Quantification module. This multi-purpose module is ideally suited for analysis of lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung. Take your vacuole analysis to the next level with measurements including total and average vacuole area, average vacuole diameter and perimeter, and average vacuoles per cell, along with optional per-object data. Leverage this module’s customizable graphing options to summarize and share your data by generating publication-ready histograms of vacuole diameter and area.

Try it out! Click here to initiate your free proof-of-concept HALO image analysis.

File formats supported by the HALO image analysis platform:

Non-proprietary (JPG, TIF, OME.TIFF)
Nikon (ND2)
3D Histech (MRXS)
Akoya (QPTIFF, component TIFF)
Olympus / Evident (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Ventana (BIF)
Philips (iSyntax, i2Syntax)
KFBIO (KFB, KFBF)
DICOM (DCM*)
*whole-slide images

Announcing the Vacuole Quantification module v3.2

We’re excited to announce the launch of the updated Vacuole Quantification module v3.2! In this blog post, you can learn about some of the new

Publication Spotlight

The table below includes publications that cite the Vacuole Quantification module.

Your publication not on the list? Drop us an email to let us know about it!

Title	Authors	Year	Journal	Application	HALO Modules	Product
A suboptimal maternal diet combined with accelerated postnatal growth results in an altered aging profile in the thymus of male rats	Tarry-Adkins JL, Aiken CE, Ashmore TJ, Fernandez-Twinn DS, Chen J-H, Ozanne SE	2018	The FASEB Journal	Neuroscience, Metabolism	Classifier, Vacuole	HALO
Phenotypic characterization of Adig null mice suggests roles for adipogenin in the regulation of fat mass accrual and leptin secretion	Alvarez-Guaita A, Patel S, Lim K, Haider A, Dong L, Conway OJ, Ma MKL, Chiarugi D, Saudek V, O'Rahilly S, Savage DB	2021	Cell Reports	Metabolism	Vacuole	HALO
Breast adipocyte size associates with ipsilateral invasive breast cancer risk after ductal carcinoma in situ	Almekinders MMM, Schaapveld M, Thijssen B, Visser LL, Bismeijer T, Sanders J, Isnaldi E, Hofland I, Mertz M, Wessels LFA, Broeks A, Hooijberg E, Zwart W, Lips EH, Grand Challenge PRECISION Consortium, Desmedt C, Wesseling J	2021	npj Breast Cancer	Oncology	Vacuole	HALO
Allostatic hypermetabolic response in PGC1?/? heterozygote mouse despite mitochondrial defects	Rodriguez-Cuenca S, Lelliot C, Campbell M, Peddinti G, Martinuez-U√ía M, Ingvorsen C, Dias A, Relat J, Mora S, HyÀÜtyl‚Ä∞inen T, Zorzano A, Ore√∂i? M, Bjursell M, Bohlooly-Y M, Lind√àn D, Vidal-Puid A	2021	The FASEB Journal	Metabolism	Vacuole	HALO
AST-120 Treatment Alters the Gut Microbiota Composition and Suppresses Hepatic Triglyceride Levels in Obese Mice	Hiraga Y, Kubota T, Katoh, M, et al.	2021	Endocrine Research	Metabolism	Vacuole	HALO
Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity	Petkevicius K, Virtue S, Bidault G, Jenkins B, Cubuk C, Morgantini C, Aouadi M, Dopazo J, Serlie M, Koulman A, Vidal-Puig A	2019	Elife		Classifier, Vacuole	HALO
Evaluation of a PEGylated Fibroblast Growth Factor 21 Variant Using Novel Preclinical Magnetic Resonance Imaging and Magnetic Resonance Elastography in a Mouse Model of Nonalcoholic Steatohepatitis	Tang H, Li J, Zinker B, Boehm S, Mauer A, Rex-Rabe S, Glaser K, Fronheiser M, Bradstreet T, Nakao Y, Petrone T, Pena A, Villano M, Chow P, Malhi H, Charles E, Hayes W, Ehman R, Du S, Yin M	2022	Journal of Magnetic Resonance Imaging	Metabolism	Vacuole	HALO
Obesity due to Steroid Receptor Coactivator-1 deficiency is associated with endocrine and metabolic abnormalities	Cacciottolo T, Henning E, Keogh J, Lassen P, Lawler K, Bounds R, Ahmed R, Perdikari A, Mendes de Oliverira E, Smith M, Godfrey E, Johnson E, Hodson L, Clement K, van der Klaauw A, Farooqi I	2022	The Journal of Clinical Endocrinology & Metabolism	Metabolism	Vacuole	HALO
Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice	Patel S, Haider A, Alvarez-Guaita A, Bidault G, Moustafa J, Guiu-Jurado E, Tadross J, Warner J, Harrison J, Virtue S, Scurria F, Zvetkova I, Bluher M, Small K, O'Rahilly S, Savage D	2022	Molecular Metabolism	Metabolism	Vacuole	HALO
Defective extracellular matrix remodeling in brown adipose tissue is associated with fibro-inflammation and reduced diet-induced thermogenesis	Pellegrinelli V, Figueroa-Juárez E, Samuelson I, U-Din M, Rodriguez-Fdez S, Virtue S, Leggat J, Çubuk C, Peirce V, Niemi T, Campbell M, Rodriguez-Cuenca S, Dopazo Blázquez J, Carobbio S, Virtanen K, Vidal-Puig A	2023	Cell Reports	Metabolism	Classifier, Area Quantification, Vacuole	HALO
Skeletal phenotype amelioration in Mucopolysaccharidosis VI requires intervention at the earliest stages of postnatal development	Hwang-Wong E, Amar G, Das N, Zhang X, Aaron N, Gale K, Rothman N, Fante M, Baik A, Bhargava A, Fricker A, McAlister M, Rabinowitz J, Lees-Shepard J, Nannuru K, Economides AN, Cygnar KD	2023	JCI Insight	Other	Vacuole, Layer Thickness	HALO
A monoclonal antibody activating AdipoR for type 2 diabetes and nonalcoholic steatohepatitis	Asahara N, Okada-Iwabu M, Iwabu M, Wada K, Oka K, Yamauchi T, Kadowaki T	2023	Science Advances	Gastroenterology	Vacuole	HALO

Related HALO Modules

Tissue Classifier Add-on

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

Learn More