Axon Quantification

AXON

Product Overview

The Axon module counts and measures axons and myelin areas in nerve cross-sections. Since axon cross-sections are irregularly shaped, the algorithm uses image processing techniques to detect each axon’s minimum and median diameter. Once each axon has been measured the software generates important statistics including the average inner and outer diameters, average G-Ratio, average perimeter, total axon area, total myelin area, and average myelin thickness. Additionally, it produces several histograms including an axon G-Ratio histogram.

In the example shown above, the inside of each axon (the non-myelin positive area) is highlighted red and the outside boundary of each axon is demarcated with a yellow outline.

The Axon Quantification module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

AXON

The Axon module counts and measures axons and myelin areas in nerve cross-sections. Since axon cross-sections are irregularly shaped, the algorithm uses image processing techniques to detect each axon’s minimum and median diameter. Once each axon has been measured the software generates important statistics including the average inner and outer diameters, average G-Ratio, average perimeter, total axon area, total myelin area, and average myelin thickness. Additionally, it produces several histograms including an axon G-Ratio histogram.

In the example shown above, the inside of each axon (the non-myelin positive area) is highlighted red and the outside boundary of each axon is demarcated with a yellow outline.

Here are a few publications that cite use of the Axon module.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Intrathecal Administration of AAV/GALC Vectors in 10- 11-Day-Old Twitcher Mice Improves Survival and Is Enhanced by Bone Marrow Transplant Karmuthil-Melethil S, Marshall MS, Heindel C, Jakubauskas B, Bongarzone ER, Gray SJ2016Journal of Neuroscience ResearchNeuroscienceAxon
Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury Stewart A, Glaser E, Mott C, Bailey W, Sullivan P, Patel S, Gensel J
2022Journal of Neurotrauma
Neuroscience
Axon
Sensory neurons display cell-type-specific vulnerability to loss of neuron-glia interactionsElbaz B, Yang L, Vardy M, Isaac S, Rader B, Kawaguchi R, Traka M, Woolf C, Renthal W, Popko B2022Cell ReportsNeuroscienceAxon

Click here to initiate your free proof-of-concept HALO® image analysis.