The Axon module counts and measures axons and myelin areas in nerve cross-sections. Since axon cross-sections are irregularly shaped, the algorithm uses image processing techniques to detect each axon’s minimum and median diameter. Once each axon has been measured the software generates important statistics including the average inner and outer diameters, average G-Ratio, average perimeter, total axon area, total myelin area, and average myelin thickness. Additionally, it produces several histograms including an axon G-Ratio histogram.
In the example shown above, the inside of each axon (the non-myelin positive area) is highlighted red and the outside boundary of each axon is demarcated with a yellow outline.