Islet Quantification FL
Islet Quantification FL
Product Overview
Indica Labs’ Islet Quantification FL module counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and nuclear staining.
In the example above, pancreas sections are probed with DAPI (blue), anti-insulin antibodies detected with a red fluorophore and anti-Ki67 antibodies detected with a green fluorophore. The module identifies the outer islet boundaries based on insulin positivity. Cells within the islet boundary are then scored for insulin and Ki67 positivity. In the markup image, insulin-positive beta cells are identified with red cytoplasmic markup, Ki-67 positive cells are identified with green nuclear markup, dual-positive cells have both red and green markup, and cells with no staining have blue nuclear markup. The software reports number of islets, islet area, number of insulin, Ki-67, and dual-positive cells.
The module can also analyze two cytoplasmic markers, for example insulin and glucagon. In cases where tissues are probed for two islet markers such as glucagon and insulin, the islet boundary can be determined based on positivity for both markers
The Islet Quantification FL module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and file formats.
Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.