Muscle Fiber FL Quantification
MUSCLE FIBER FL
The Muscle Fiber FL module detects fluorescently-stained muscle fibers and reports the total count as well as the perimeter, area, and diameter of each fiber. The module also detects nuclei (and nuclear position with respect to the fiber) and reports fiber membrane dye intensity and co-localization (where applicable).
Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter and maximum diameter. Finally the module reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.
As shown in the example image above, the module detects positivity of fibers for each selected channel within the fiber compartment and/or fiber membrane. Additionally, the algorithm provides the option to detect and count nuclei, and identify fibers with centrally located nuclei. A notable increase or decrease in fibers with centrally located nuclei can be indicative of certain myopathic findings.