Muscle Fiber Quantification

All Applications, Brightfield, Cell-Based Analysis, Toxicologic Pathology

MUSCLE FIBER

Product Overview

The Muscle Fiber module detects fibers and reports the total count of muscle fibers as well as the perimeter, area, and diameter of each fiber.

Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally the software reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.

As in the example shown above, the module is capable of detecting unstained muscle fibers where fiber basement membranes are stained, in this case with Laminin. It can also be configured to detect nuclei and identify fibers with centrally located nuclei. A notable increase or decrease in fibers with centrally located nuclei can be indicative of certain myopathic findings.

The Muscle Fiber module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

MUSCLE FIBER

The Muscle Fiber module detects fibers and reports the total count of muscle fibers as well as the perimeter, area, and diameter of each fiber.

Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally the module reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.

Here are a few publications that cite use of our Muscle Fiber module. Your publication not on the list? Drop us an email to let us know about it!

Title	Authors	Year	Journal	Application	HALO Modules
Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice	Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky TB, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy AJ, Yancopoulos GD, Gromada J, Stitt T	2015	Skeletal Muscle	Myology	Muscle Fiber
Peripheral androgen receptor gene suppression rescues disease in mouse models of spinal and bulbar muscular atrophy	Lieberman AP, Yu Z, Murray S, Peralta R, Low A, Guo S, Yu XX, Cortes CJ, Bennett CF, Monia BP, La Spada AR, Hung G	2014	Cell Reports	Myology	Muscle Fiber
Development of antibody-siRNA conjugate targeted to cardiac and skeletal muscles	Sugo T, Terada M, Oikawa T, Miyata K, Nishimura S, Kenjo E, Ogasawara-Shimizu M, Makita Y, Imaichi S, Murata S, Kentaro Otaka, Kikuchi K, Teratani M, Masuda Y, Kamei T, Takagahara S, Ikeda S, Ohtaki T, Matsumoto H	2016	The Journal of Controlled Release	Myology	Cytonuclear, Muscle Fiber
Inhibition of myostatin prevents microgravity-induced loss of skeletal muscle mass and strength	Smith RC, Cramer MS, Mitchell PJ, Lucchesi J, Ortega AM, Livingston EW, Ballard D, Zhang L, Hanson J, Barton K, Berens S, Credille KM, Bateman TA, Ferguson VL, Ma YL, Stodieck LS	2020	PLOS One	Myology	Muscle Fiber
Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration	Giannakis N, Sansbury BE, Patsalos A, Hays TT, Riley CO, Han X, Spite M, Nagy L	2019	Nature Immunology	Immunology, Myology	Muscle Fiber
The Goto Kakizaki rat: Impact of age upon changes in cardiac and renal structure, function	Meagher P, Civitarese R, Lee X, Gordon M, Bugyei-Twum A, Desjardins J, Kabir G, Zhang Y, Kosanam H, Visram A, Leong-Poi H, Advani A, Connelly K	2021	PLOS ONE	Myology	Muscle Fiber
A growth factor–expressing macrophage subpopulation orchestrates regenerative inflammation via GDF-15	Patsalos A, Halasz L, Medina-Serpas M, Berger W, Daniel B, Tzerpos P, Kiss M, Nagy G, Fischer C, Simandi Z, Varga T, Nagy L	2021	Journal of Experimental Medicine		Muscle Fiber
Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in mice	Kenjo E, Hozumi H, Makita Y, Iwabuchi K, Fujimoto N, Matsumoto S, Kimura M, Amano Y, Ifuku M, Naoe Y, Inukai N, Hotta A	2021	Nature Communications	Other	Muscle Fiber

Click here to initiate your free proof-of-concept HALO® image analysis.

brightfield, cell-based analysis, IHC, muscle, tox pathology

Muscle Fiber Quantification

MUSCLE FIBER

Product Overview

MUSCLE FIBER

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