Muscle Fiber Quantification
The Muscle Fiber module detects fibers and reports the total count of muscle fibers as well as the perimeter, area, and diameter of each fiber.
Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally the software reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.
As in the example shown above, the module is capable of detecting unstained muscle fibers where fiber basement membranes are stained, in this case with Laminin. It can also be configured to detect nuclei and identify fibers with centrally located nuclei. A notable increase or decrease in fibers with centrally located nuclei can be indicative of certain myopathic findings.