Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

Muscle Fiber Quantification

MUSCLE FIBER

 

Product Overview

The Muscle Fiber module detects fibers and reports the total count of muscle fibers as well as the perimeter, area, and diameter of each fiber.

Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally the software reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.

As in the example shown above, the module is capable of detecting unstained muscle fibers where fiber basement membranes are stained, in this case with Laminin. It can also be configured to detect nuclei and identify fibers with centrally located nuclei. A notable increase or decrease in fibers with centrally located nuclei can be indicative of certain myopathic findings.

The Muscle Fiber module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

MUSCLE FIBER

The Muscle Fiber module detects fibers and reports the total count of muscle fibers as well as the perimeter, area, and diameter of each fiber.

Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally the module reports the diameter (average/minimum/maximum), the average perimeter length, and the average fiber area for all fibers in the entire section.

As in the example shown above, the module is capable of detecting unstained muscle fibers where fiber basement membranes are stained, in this case with Laminin. It can also be configured to detect nuclei and identify fibers with centrally located nuclei. A notable increase or decrease in fibers with centrally located nuclei can be indicative of certain myopathic findings.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Here are a few publications that cite use of our Muscle Fiber module.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky TB, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy AJ, Yancopoulos GD, Gromada J, Stitt T2015Skeletal MuscleMyologyMuscle Fiber
Peripheral androgen receptor gene suppression rescues disease in mouse models of spinal and bulbar muscular atrophy Lieberman AP, Yu Z, Murray S, Peralta R, Low A, Guo S, Yu XX, Cortes CJ, Bennett CF, Monia BP, La Spada AR, Hung G2014Cell ReportsMyologyMuscle Fiber
Development of antibody-siRNA conjugate targeted to cardiac and skeletal muscles Sugo T, Terada M, Oikawa T, Miyata K, Nishimura S, Kenjo E, Ogasawara-Shimizu M, Makita Y, Imaichi S, Murata S, Kentaro Otaka, Kikuchi K, Teratani M, Masuda Y, Kamei T, Takagahara S, Ikeda S, Ohtaki T, Matsumoto H2016The Journal of Controlled ReleaseMyologyCytonuclear, Muscle Fiber
Inhibition of myostatin prevents microgravity-induced loss of skeletal muscle mass and strengthSmith RC, Cramer MS, Mitchell PJ, Lucchesi J, Ortega AM, Livingston EW, Ballard D, Zhang L, Hanson J, Barton K, Berens S, Credille KM, Bateman TA, Ferguson VL, Ma YL, Stodieck LS2020PLOS OneMyologyMuscle Fiber
Dynamic changes to lipid mediators support transitions among macrophage subtypes during muscle regeneration Giannakis N, Sansbury BE, Patsalos A, Hays TT, Riley CO, Han X, Spite M, Nagy L2019Nature ImmunologyImmunology, MyologyMuscle Fiber

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.