Spatial Analysis

SPATIAL ANALYSIS

 

Product Overview

Particularly useful to those involved in the immune-oncology field, the Spatial Analysis module offers a suite of subsequent analysis tools which can be used to identify proximity and relative spatial distribution of objects, cells, and/or features across single tissues or serial sections. This module is embedded within the HALO platform and can be used in conjunction with any of our cell-based analysis modules for brightfield or fluorescence.

 


Nearest Neighbor Analysis

Determine the average distance and number of unique neighbors between any two cell or object populations.  The example below depicts pan cytokeratin positive cells (blue) and CD4 positive cells (red). Grey lines connect each CD4 positive cell to the nearest pan cytokeratin positive cell.


Proximity Analysis

Calculate the number of objects or cells within a certain distance of another object or cell.  In the example below, the distance is set to 30 microns. CD8 positive cells within 30 microns of a pan cytokeratin positive cell (blue) are labeled green and CD8 positive cells greater than 30 microns from pan cytokeratin positive cell are labeled purple. A corresponding proximity histogram is automatically generated.


Infiltration Analysis

Determine the number of objects or cells within a set range of an annotated region of interest.  Depicted below is a whole slide image stained with CD8. The tumor boundary has been defined/annotated in green.  The infiltration analysis tool defines the invasive margin inside of tumor (yellow and red) and the invasive margin outside of the tumor (blue and purple) automatically. In this example, the distance is set to 500 microns around the annotated tumor boundary.  CD8 positive cells are quantified within the margin. A histogram is automatically generated to reflect CD8+ cell density inside the tumor boundary (-1 to -500), at the tumor boundary (0), and outside the tumor boundary (+1 to +500) as shown in the image below.



Density Heatmaps

The density heatmap spatial analysis algorithm measures the density of a selected cell population from your object data within a certain radius. The scaling and colors of the markup are customizable. Reported in the summary data, the entire tissue area is analyzed and all cells of the desired population are counted. The average cell population and the average cell population density is calculated within the user-defined radius, as well as the minimum and maximum densities.

The tissue below is stained with an immune cell marker (red stain) and a tumor marker (DAB stain).  The image was analyzed with the Multiplex IHC module to detect positivity and produce object data. The density heatmap analysis was used with a radius of 25 microns, measuring the density of immune cell positivity across the whole slide. The markup image, with adjustable transparency, depicts areas of higher density in red and orange, moderate density in yellow, and areas of lower density in green and blue.

 

The Spatial Analysis module integrates seamlessly into the HALO® platform and is compatible with a number of file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

SPATIAL ANALYSIS

Product Overview

Particularly useful to those involved in the immune-oncology field, the Spatial Analysis module offers a suite of subsequent analysis tools which can be used to identify proximity and relative spatial distribution of objects, cells, and/or features across single tissues or serial sections. This module is embedded within the HALO platform and can be used in conjunction with any of our cell-based analysis modules for brightfield or fluorescence.

Nearest Neighbor Analysis

Determine the average distance and number of unique neighbors between any two cell or object populations.  The example below depicts pan cytokeratin positive cells (blue) and CD4 positive cells (red). Grey lines connect each CD4 positive cell to the nearest pan cytokeratin positive cell.
NearestNeighbor

Proximity Analysis

Calculate the number of objects or cells within a certain distance of another object or cell.  In the example below, the distance is set to 30 microns. CD8 positive cells within 30 microns of a pan cytokeratin positive cell (blue) are labeled green and CD8 positive cells greater than 30 microns from pan cytokeratin positive cell are labeled purple. A corresponding proximity histogram is automatically generated.
CD4_Proximity

Infiltration Analysis

Determine the number of objects or cells within a set range of an annotated region of interest.  Depicted below is a whole slide image stained with CD8. The tumor boundary has been defined/annotated in green.  The infiltration analysis tool defines the invasive margin inside of tumor (yellow and red) and the invasive margin outside of the tumor (blue and purple) automatically. In this example, the distance is set to 500 microns around the annotated tumor boundary.  CD8 positive cells are quantified within the margin. A histogram is automatically generated to reflect CD8+ cell density inside the tumor boundary (-1 to -500), at the tumor boundary (0), and outside the tumor boundary (+1 to +500) as shown in the image below.


infiltration_Histogram

Density Heatmaps

The density heatmap spatial analysis algorithm measures the density of a selected cell population from your object data within a certain radius. The scaling and colors of the markup are customizable. Reported in the summary data, the entire tissue area is analyzed and all cells of the desired population are counted. The average cell population and the average cell population density is calculated within the user-defined radius, as well as the minimum and maximum densities.

The tissue below is stained with an immune cell marker (red stain) and a tumor marker (DAB stain).  The image was analyzed with the Multiplex IHC module to detect positivity and produce object data. The density heatmap analysis was used with a radius of 25 microns, measuring the density of immune cell positivity across the whole slide. The markup image, with adjustable transparency, depicts areas of higher density in red and orange, moderate density in yellow, and areas of lower density in green and blue.

Here are a few publications that cite the use of our HALO Spatial Analysis module. Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Neoadjuvant anti-PD-1 immunotherapy promotes a survival benefit with intratumoral and systemic immune responses in recurrent glioblastoma Cloughesy TF, Mochizuki AY, Orpilla JR, Hugo W, Lee AH, Davidson TB, Wang AC, Ellingson BM, Rytlewski JA, Sanders CM, Kawaguchi ES, Du L, Li G, Yong WH, Gaffey SC, Cohen AL, Mellinghoff IK, Lee EQ, Reardon DA, O’Brien BJ, Butowski NA, Nghiemphu PL, Clarke JL, Arrillaga-Romany IC, Colman H, Kaley TJ, de Groot JF, Liau LM, Wen PY, Prins RM2019Nature MedicineOncology, Immuno-oncologySpatial Analysis, Highplex FL
Multidimensional, quantitative assessment of PD-1/PD-L1 expression in patients with Merkel cell carcinoma and association with response to pembrolizumab Giraldo NA, Nguyen P, Engle EL, Kaunitz GJ, Cottrell TR, Berry S, Green B, Soni A, Cuda JD, Stein JE, Sunshine JC, Succaria F, Xu H, Ogurtsova A, Danilova L, Church CD, Miller NJ, Fling S, Lundgren L, Ramchurren N, Yearley JH, Lipson EJ, Cheever M, Anders RA, Nghiem PT, Topalian SL, Taube JM2018Journal for ImmunoTherapy of CancerOncology, Immuno-oncologySpatial Analysis, Serial Section
Precision immunoprofiling by image analysis and artificial intelligenceKoelzer VH, Sirinukunwattana K, Rittscher J, Mertz KD2019Vichows ArchivReviewClassifier, Multiplex IHC, Spatial Analysis
Spatial immune profiling of the colorectal tumor microenvironment predicts good outcome in stage II patientsNearchou IP, Gwyther BM, Georgiakakis ECT, Gavriel CG, Kajiwara Y, Ueno H, Harrison DJ, Caie PD2020Digital MedicineImmuno-oncologyClassifier, Area Quantification, Spatial Analysis
Locally confined IFNɣ production by CD4+ T cells provides niches for murine cytomegalovirus
4 replication in the salivary gland
Oderbolz J, Zangger N, Zimmerman L, Sandu I, Starruß J, Graw F, Oxenius A2021bioRxivClassifier, Spatial Analysis, Highplex FL
Genetic alterations and expression characteristics of ARID1A impact tumor immune contexture and survival in early-onset gastric cancerZou J, Qin W, Wang L, Wang Y, Shen J, Xiong W, Yu S, Song S, Ajani JA, Lin S-Y, Mills GB, Yuan X, Chen J, Peng G2020American Journal of Cancer ResearchImmuno-oncologySpatial Analysis, Highplex FL
Activated Regulatory T-Cells, Dysfunctional and SenescentT-Cells Hinder the Immunity in Pancreatic CancerSivakumar S, Abu-Shah E, Ahern DJ, Arbe-Barnes EH, Jainarayanan AK, Mangal N, Reddy S, Rendek A, Easton A, Kurz E, Silva M, Soonawalla Z, Heij LR, Bashford-Rogers R, Middleton MR, DUstin ML2021cancersClassifier, Multiplex IHC, Spatial Analysis, Highplex FL
The immune suppressive microenvironment affects efficacy of radio-immunotherapy in brain metastasisNiesel K, Schulz M, Anthes J, Alekseeva T, Macas J, Salamero-Boix A, Mockl A, Oberwahrenbrock T, Lolies M, Stein S, Plate KH, Reiss Y, Rodel F, Sevenich L2021EMBO Molecular MedicineImmuno-oncologySpatial Analysis, Highplex FL
Spatially organized multicellular immune hubs in human colorectal cancerPelka K, Hofree M, Chen J, Sarkizova S, Pirl J, Jorgji V, Bejnood A, Dionne D, Ge W, Xu K, Chao S, Zollinger D, Lieb D, Reeves J, Fuhrman C, Hoang M, Delorey T, Nguyen L, Waldman J, Klapholz M, Wakiro I, Cohen O, Albers, J, Smillie C, Cuoco M, Wu J, Su M, Yeung J, Vijaykumar B, Magnuson A, Asinovski N, Moll T, Goder-Reise M, Applebaum A, Brais L, DelloStritto L, Denning S, Phillips S, Hill E, Meehan J, Frederick D, Sharova T, Kanodia A, Todres E, Jane-Valbuena J, Biton M, Izar B, Lambden C, Clancy T, Bleday R, Melnitchouk N, Irani J, Kunitake H, Berger D, Srivastava A, Hornick J, Ogino S, Rotem A, Vigneau S, Johnson B, Corcoran R, Sharpe A, Kuchroo V, Ng K, Giannakis M, Nieman L, Boland G, Arguirre A, Anderson A, Rosenblatt-Rosen O, Regev A, Hachohen N2021CellOncologySpatial Analysis, Highplex FL
Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced ArthritisYang F, Luo X, Zhu W, Li J, Zheng Z, Zhu P2021Mediators of InflammationSpatial Analysis, Highplex FL
Endothelial cell infection and dysfunction, immune activation in severe COVID-19Qin Z, Liu F, Blair R, Wang C, Yang H, Mudd J, Currey J, Iwanaga N, He J, Mi R, Han K, Midkiff C, Alam M, Aktas B, Heide R, Veazey R, Piedimonte G, Maness N, Ergün S, Mauvais-Jarvis F, Rappaport J, Kolls J, Qin X2021TheranosticsImmunology, Infectious DiseaseMultiplex IHC, Spatial Analysis
Establishing standardized immune phenotyping of metastatic melanoma by digital pathologySobottka B, Nowak M, Frei A, Haberecker M, Merki S, Tumor Profiler consortium, Levesque M, Dummer R, Moch H, Koelzer V2021Laboratory InvestigationImmuno-oncologyMultiplex IHC, Spatial Analysis, HALO AI
Progranulin mediates immune evasion of pancreatic ductal adenocarcinoma through regulation of MHCI expressionCheung P, Yang J, Fang R, Borgers A, Krengel K, Stoffel A, Althoff K, Yip C, Siu E, Ng L, Lang K, Cham L, Engel D, Soun C, Cima I, Scheffler B, Striefler J, Sinn M, Bahra M, Pelzer U, Oettle H, Markus P, Smeets E, Aarntzen E, Savvatakis K, Liffers S, Lueong S, Neander C, Bazarna A, Zhang X, Paschen A, Crawford H, Chan A, Cheung S, Siveke J2022Nature CommunicationsOncologyCytonuclear, Spatial Analysis, Highplex FL
Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancerDu S, Qian J, Tan S, Li W, Liu P, Zhao J, Zeng Y, Xu L, Wang Z, Cai J2022Cancer LettersOncologyArea Quantification, Cytonuclear, Spatial Analysis, Highplex FL
The Tumor Immune Landscape and Architecture of Tertiary Lymphoid Structures in Urothelial Cancervan Dijk N, Gil-Himenez A, Silina K, van Montfoort M, Einerhand S, Jonkman L, Voskuilen C, Peters D, Sanders J, Lubeck Y, Broeks A, Hooijberg E, Vis D, van den Broek M, Wessels L, van Rhijn B, van der Heijden M2021Frontiers in ImmunologyImmuno-oncologyCytonuclear, Spatial Analysis, Highplex FL
Intratumoural spatial distribution of S100B?+?folliculostellate cells is associated with proliferation and expression of FSH and ER? in gonadotroph tumoursIlie M, Vasiljevic A, Chanal M, Gadot N, Chinezu L, Jouanneau E, Hennino A, Raverot G, Bertolino P2022Acta Neuropathologica CommunicationsOncologyCytonuclear, Spatial Analysis
Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancerLiu H, Wu J, Xu X, Wang H, Zhang C, Yin S, He Y2022International ImmunopharmacologyOncologyCytonuclear, Spatial Analysis, Highplex FL
SPOP promotes cervical cancer progress by inducing PD-1 move away from PD-L1 in spatial localizationWu J, Wu Y, Guo Q, Chen S, Wang S, Zhu J, Wu X, Ju X2022Research SquareOncologyCytonuclear, Spatial Analysis, Highplex FL, TMA
Higher proportions of CD39+ tumorresident cytotoxic T cells predict recurrence-free survival in patients with stage III melanoma treated with adjuvant immunotherapyAttrill G, Owen C, Ahmed T, Vergara I, Colebatch A, Conway J, Nahar K, Thompson J, da Silva I, Carlino M, Menzies A, Lo S, Palendira U, Scolyer R, Long G, Wilmott J2022Journal of Immuno Therapy of CancerImmuno-oncologyCytonuclear, Spatial Analysis, Highplex FL
gp96 Expression in Gliomas and Its Association with Tumor Malignancy and T Cell Infiltrating LevelLi C, Wang Y, Long L, Zhang P, Zhang Y, Ji N2022Hindawi Journal of OncologyImmuno-oncologySpatial Analysis, Highplex FL, Figure Maker
Spartalizumab or placebo in combination with dabrafenib and trametinib in patients with BRAF V600-mutant melanoma: exploratory biomarker analyses from a randomized phase 3 trial (COMBI-i)Tawbi H, Robert C, Brase J, Gusenleitner D, Gasal E, Garrett J, Savchenko A, Gorgun G, Flaherty K, Ribas A, Dummer R, Schadendorf D, Long G, Nathan P, Ascierto P2022Journal for ImmunoTherapy of CancerOncologyMultiplex IHC, Spatial Analysis
Endothelial cell death after ionizing radiation does not impair vascular structure in mouse tumor modelsKaeppler J, Chen J, Buono M, Vermeer J, Kannan P, Cheng W, Voukantsis D, Thompson J, Hill M, Allen D, Gomes A, Kersemans V, Kinchesh P, Smart S, Buffa F, Nerlov C, Muschel R, Markelc B2022EMBO ReportsOncologySpatial Analysis, Highplex FL
Approaches to spatially resolving the tumour immune microenvironment of hepatocellular carcinomaMarsh-Wakefield F, Ferguson A, Liu K, Santhakumar C, McCaughan G, Palendira U2022Therapeutic Advances in Medical OncologyReviewMultiplex IHC, Spatial Analysis, Highplex FL
Spatial Distribution and Predictive Significance of Dendritic Cells and Macrophages in Esophageal Cancer Treated With Combined Chemoradiotherapy and PD-1 BlockadeMa X, Guo Z, Wie X, Zhao G, Han D, Zhang T, Chen X Cao F, Dong J, Zhao L, Yuan Z, Wang P, Pang Q, Yan C, Zhang W2021Frontiers in ImmunologyOncologySpatial Analysis, Highplex FL
Modulation of the Human Pancreatic Ductal Adenocarcinoma Immune Microenvironment by Stereotactic Body RadiotherapyMills B, Qiu H, Drage M, Chen C, Mathew J, Garrett-Larsen J, Ye J, Uccello T, Murphy J, Belt B, Lord E, Katz A, Linehan D, Gerber S2021Clinical Cancer ResearchOncologyClassifier, Spatial Analysis, Highplex FL
Senescence-Associated Molecules and Tumor-Immune-Interactions as Prognostic Biomarkers in Colorectal CancerKellers F, Fernandez A, Konykiewitz B, Schindeldecker M, Tagscherer K, Heintz A, Jesinghaus M, Roth W, Foersch S2022Frontiers in MedicineOncologyClassifier, Multiplex IHC, Spatial Analysis, Registration, TMA
Tau Pathology in Chronic Traumatic Encephalopathy is Primarily NeuronalButler M, Dixon E, Stein T, Alvarez V, Huber B, Buckland M, McKee A, Cherry J2022Journal of Neuropathology & Experimental NeurologyNeuroscienceMultiplex IHC, Spatial Analysis, Highplex FL
Chemoradiation-induced alteration of programmed death-ligand 1, CD8+ tumor-infiltrating lymphocytes and mucin expression in rectal cancerBaretti M, Zhu Q, Fu W, Meyer J, Wang H, Anders R, Azad N2022OncotargetOncologyMultiplex IHC, Spatial Analysis
Unveiling the tumor immune microenvironment of organ-specific melanoma metastatic sitesConway J, Rawson R, Lo S, Ahmed T, Veregara I, Gide T, Attrill G, Carlino M, Saw R, Thompson J, Spillane A, Shannon K, Shivalingam B, Menzies A, Wilmott J, Long G, Scolyer R, da Silva I2022Journal for ImmunoTherapy of CancerOncologyClassifier, Spatial Analysis, Highplex FL
Haploinsufficiency of the lysosomal sialidase NEU1 results in a model of pleomorphic rhabdomyosarcoma in miceMachado E, van de Vlekkert D, Sheppard H, Perry S, Downing S, Laxton J, Ashmun R, Finkelstein D, Neale G, Hu H, Harwood F, Koo S, Grosveld G, d'Azzo A2022Communications BiologyOncologyMembrane, Spatial Analysis
Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin systemWeigert A, Zheng X, Nenzel A, Turkowski K, Gunther S, Strack E, Sirait-Fischer E, Elwakeel E, Kur I, Nikam V, Valasarajan C, Winter H, Wissgott A, Voswinkel R, Grimminger F, Brune B, Seeger W, Pullamsetti S, Savai R2022Nature CommunicationsOncologySpatial Analysis, Highplex FL
Use of High-Plex Data Reveals Novel Insights into the Tumour Microenvironment of Clear Cell Renal Cell CarcinomaDe Filippis R, Wolflein G, Um I, Caie P, Warren S, White A, Suen E, To E, Arandjelovic O, Harrison D2022CancersOncologySpatial Analysis
Systemic Nos2 Depletion and Cox inhibition limits TNBC disease progression and alters lymphoid cell spatial orientation and densitySomasundaram V, Ridnour L, Cheng R, Walke A, Kedei N, Bhattacharyya D, Wink A, Edmondson E, Butcher D, Warner A, Dorsey T, Scheiblin D, Heinz W, Bryant R, Kinders R, Lipkowitz S, Wong S, Pore M, Hewitt S, McVicar D, Anderson S, Chang J, Glynn S, Ambs S, Lockett S, Wink D2022Redox BiologyOncologyClassifer, ISH/FISH, Spatial Analysis, Registration, ISH-IHC/FISH-IF
Annexin A2/TLR2/MYD88 pathway induces arginase 1 expression in tumor-associated neutrophilsZhang H, Zhu X, Friesen T, Kwak J, Pisarenko T, Mekvanich S, Velasco M, Randolph T, Kargl J, Houghton A2022Journal of Clinical InvestigationImmuno-oncologySpatial Analysis, ISH-IHC/FISH-IF
Heterogeneity of programmed death-ligand 1 expression and infiltrating lymphocytes in paired resected primary and metastatic non-small cell lung cancerWu J, Sun W, Yang X, Wang H, Liu X, Chi K, Zhou L, Huang X, Mao L, Zhao S, Ding T, Meng B, Lin D2021Modern PathologyImmuno-oncologySpatial Analysis, Highplex FL
Whole-slide imaging, tissue image analysis, and artificial intelligence in veterinary pathology: An updated introduction and reviewZuraw A, Aeffner F2021Veterinary PathologyReviewSpatial Analysis
Single-cell profiling of tumor-infiltrating TCF1/TCF7+ T cells reveals a T lymphocyte subset associated with tertiary lymphoid structures/organs and a superior prognosis in oral cancerPeng Y, Xiao L, Rong H, Ou Z, Cai T, Liu N, Li B, Zhang L, Wu F, Lan T, Lin X, Li Q, Ren S, Fan S, Li J2021Oral OncologyOncologySpatial Analysis, Highplex FL
Impact of HPV status on immune responses in head and neck squamous cell carcinomaQureshi H, Zhu X, Yang G, Steadele M, Pierce R, Futran N, Lee S, Mendez E, Houghton A2022Oral OncologyOncologyMultiplex IHC, Spatial Analysis, Highplex FL
Developing a Nomogram for Preoperative Prediction of Cervical Cancer Lymph Node Metastasis by Multiplex ImmunofluorescenceWu J, Guo Q, Zhu J, Wu Y, Liang S Chen S, Wang S, Ju X, Wu X2022AuthoreaOncologySpatial Analysis, Highplex FL, TMA
Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancerRamos R, Missolo-Koussou Y, Gerber-Ferder Y, Bromley C, Bugatti M, Nunez N, Boari J, Richer W, Menger L, Denizeau J, Sedlik C, Caudana P, Kotsias F, Niborski L, Viel S, Bohec M, Lameiras S, Baulande S, Lesage L, Nicolas A, Meseure D, Vincent-Salomon A, Reyal F, Dutertre C, Ginhoux F, Vimeux L, Donnadieu E, Buttard B, Galon J, Zelenay S, Vermi W, Guermonprez P, Piaggio E, Helft J2022CellImmuno-oncologyClassifier, Spatial Analysis, Highplex FL
Neoantigen-specific CD4+ T cells in human melanoma have diverse differentiation states and correlate with CD8+ T cell, macrophage, and B cell functionVeatch J, Lee S, Shasha C, Singhi N, Szeto J, Moshiri A, Kim T, Smythe K, Kong P, Fitzgibbon M, Jesernig B, Bhatia S, Tykodi S, Hall E, Byrd D, Thompson J, Pillarisetty V, Duhen T, Houghton A, Newell E, Gottardo R, Riddell S2022Cancer CellImmuno-oncologySpatial Analysis, Highplex FL
Escherichia coli-specific CXCL13-producing TFH are associated with clinical efficacy of neoadjuvant PD-1 blockade against muscle-invasive bladder cancerGoubet A, Lordello L, Silva C, Peguillet I, Gazzano M, Mbogning-Fonkou M, Thelemaque C, Lebacle C, Thibault C, Audenet F, Pignot G, Gravis G, Helissey C, Campbedel L, Roupret M, Xylinas E, Ouzaid I, Dubuisson A, Mazzenga M, Flament C, Ly P, Marty V, Signolle N, Sauvat A, Sbarrato T, Filahi M, Davin C, Haddad G, Khalil J, Bleriot C, Danlos F, Dunsmore G, Mulder K, Silvin A, Raoult T, Archambaud B, Belhechmi S, Boneca I, Cayet N, Moya-Nilges M, Mallet A, Daillere R, Rouleau E, Radulescu C, Allory Y, Fieschi J, Rouanne M, Ginhoux F, Le Teuff G, Derosa L, Marabelle A, Van Dorp J, Van Dijk N, van der Heijden M, Besse B, Andre F, Merad M, Kroemer G, Scoazec J, Zitvogel L, Loriot Y2022Cancer DiscoveryOncologyMultiplex IHC, Spatial Analysis
Upfront FOLFOXIRI plus bevacizumab with or without atezolizumab in the treatment of patients with metastatic colorectal cancer (AtezoTRIBE): a multicentre, open-label, randomised, controlled, phase 2 trialAntoniotti C, Rossini D, Pietrantonio F, Catteau A, Salvatore L, Lonardi S, Boquet I, Tamberi S, Marmorino F, Moretto R, Ambrosini M, Tamburini E, Tortora G, Passardi A, Bergamo F, Kassambara A, Sbarrato T, Morano F, Ritorto G, Borelli B, Boccaccino A, Conca V, Giordano M, Ugolini C, Fieschi J, Papadopulos A, Massoue C, Aprile G, Antonyzzo L, Gelsomino F, Martinelli E, Pella N, Masi G, Fontanini G, Boni L, Galon J, Cremolini C2022The Lancet OncologyOncologyMultiplex IHC, Spatial Analysis
Plasma CD27, a surrogate of the intratumoral CD27-CD70 interaction, correlates with immunotherapy resistance in renal cell carcinomaBenhamouda N, Sam I, Epaillard N, Gey A, Phan L, Pham H, Gruel N, Saldmann A, Pineau J, Hasan M, Quiniou V, Nevoret C, Verkarre V, Libri V, Mella S, Granier C, Broudin C, Ravel P, De Guillebon E, Mauge L, Helley D, Jabla B, Chaput N, Albiges L, Katsahian S, Adam J, Mejean A, Adotevi O, Vano Y, Oudard S, Tartour E2022Clinical Cancer ResearchOncologySpatial Analysis, Highplex FL
High endothelial venules associated with better prognosis in esophageal squamous cell carcinomaLi H, Tang L, Han X, Zhong L, Gao W, Chen Y, Huang J, Wen Z2022Annals of Diagnostic PathologyOncologyObject Colocalization, Spatial Analysis, Highplex FL
Defactinib, pembrolizumab, and gemcitabine in patients with advanced treatment refractory pancreatic cancer: A phase I, dose escalation, and expansion studyWang-Gillam A, Lim K, McWilliams R, Suresh R, Lockhart A, Brown A, Breden M, Belle J, Herndon J, Bogner S, Pedersen K, Tan B, Boice N, Acharya A, Abdiannia M, Gao F, Yoon H, Zhu M, Trikalinos N, Ratner L, Aranha O, Hawkins W, Herzog B, DeNardo D2022Clinical Cancer ResearchImmuno-oncologyMultiplex IHC, Spatial Analysis
Th1-involved immune infiltrates improve neoadjuvant chemoradiotherapy response of esophageal squamous cell carcinomaYuan J, Weng Z, Tan Z, Luo K, Zhong J, Xie X, Qu C, Lin X, Yang H, Wen J, Fu J2022Cancer LettersImmuno-oncologyMultiplex IHC, Spatial Analysis
Chronic rhinosinusitis patients display an aberrant immune cell localisation with enhanced S. aureus biofilm metabolic activity and biomassShaghayegh G, Cooksley C, Bouras G, Panchatcharam B, Idrizi R, Jana M, Ellis S, Psaltis A, Wormald P, Vreugde S2022Journal of Allergy and Clinical ImmunologyImmunology, Infectious DiseaseClassifier, Spatial Analysis, Highplex FL
Autologous T cell therapy for MAGE-A4+ solid cancers in HLA-A*02+ patients: a phase 1 trialHong D, Van Tine B, Biswas S, McAlpine C, Johnson M, Olszanski A, Clarke J, Araujo D, Blumenschein G, Kebriaei P, Lin Q, Tipping A, Sanderson J, Wang R, Trivedi T, Annareddy T, Bai J, Rafail S, Sun A, Fernandes L, Navenot J-M, Bushman F, Everett J, Karadeniz D, Broad R, Isabelle M, Naidoo R, Bath N, Betts G, Wolchinsky Z, Batrakou D, Van Winkle E, Elefant E, Ghobadi A, Cashen A, Grand'Maison A, McCarthy P, Fracasso P, Norry E, Williams D, Druta M, Liebner D, Odunsi K, Butler M2023Nature MedicineImmuno-oncologyISH/FISH, Spatial Analysis, Highplex FLHALO

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