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TMA (Tissue Microarray) Add-On

The TMA Add-on provides a productivity-enhancing workflow for tissue microarray analysis by enabling automated, high-throughput segmentation and batch analysis of whole slide TMA images. The add-on can be used in conjunction with any of our cell-based analysis modules for brightfield or fluorescence and can be used with HALO AI or the Tissue Classifier Add-on for tumor detection. In combination, these tools enable automatic identification of tumor and quantify cell-based IHC, ISH, or immunofluorescence assays within just the relevant tumor regions, eliminating the need for manual exclusion of non-tumor regions.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • KFBIO (KFB, KFBF)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the TMA (Tissue Microarray) Add-on. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalTopicsHALO ModulesProducts
Concurrent loss of the Y chromosome in cancer and T cells impacts outcomeChen X, Shen Y, Choi S, Abdel-Hafiz H A, Basu M, Hoelzen L, Tufano M, Mani S K, Ranjpour M, Zhu J, Ramanujan V K, Koltsova E K, Calsavara V F, Knott S R V, Theodorescu D2025NatureOncologyFISH, Highplex FL, TMAHALO
Discovery of BMS-986408, a First-In-Class Dual DGKα and DGKζ Inhibitor That Unleashes PD-1 Checkpoint and CAR T-Cell ImmunotherapiesWichroski M, Liu S-Q, Zasadil L M, Benci J L, Gedeon P C, Condon K J, Joshi S, Posy S, Carlson P, Maier A, Shen J, Kureshi R, Amako Y, Wang T, Powles R L, Li Y, Lai T, Katsyv I, Qiu H, Qi H, Wong J, Zhao D, Banas D, Onorato J, Locke G, Chen X, Chou W-C, Cook E, Witt A E, Barbieri C M, Zhang H, Olsen J B, Font-Tello A, Drokhlyansky E, Grünenfelder D C, Chupak L, Longmire T A, Jones J C, Hollmann T J, Kugler D G, Feder J N, Bueno R, Wain J, Sivakumar P, Liu Y, Dougan S K, Paweletz C P, Barbie D A, Lees E2025Cancer Immunology ResearchImmuno-oncologyHighplex FL, TMAHALO
Mesenchymal stromal cells induce neutrophil aggregation and extracellular vesicle storms for systemic lupus erythematosusOu Q, Niu L, Wang D, Yao G, Ren Q, Li Z, Mao X, Teng W, Chen Z, Xiang A, Shi S, Sun L2025Signal Transduction and Targeted TherapyImmunologyMultiplex IHC, TMAHALO
ERBB3 overexpression is enriched in diverse patient populations with castration-sensitive prostate cancer and is associated with a unique AR activity signatureVellky J E, Kirkpatrick B J, Gutgesell L C, Morales M, Brown R M, Wu Y, Maienschein-Cline M, Notardonato L D, Weinfeld M S, Nguyen R H, Brister E, Sverdlov M, Liu L, Xu Z, Kregel S, Nonn L, Vander Griend D J, Reizine N M2024Clinical Cancer ResearchOncologyArea Quantification, TMAHALO, HALO AI
Single-cell and spatial multi-omics highlight effects of anti-integrin therapy across cellular compartments in ulcerative colitisMennillo E, Kim Y, Lee G, Rusu I, Patel R K, Dorman L C, Flynn E, Li S, Bain J L, Andersen C, Rao A, Tamaki S, Tsui J, Shen A, Lotstein M L, Rahim M, Naser M, Bernard-Vazquez F, Eckalbar W, Cho S, Beck K, El-Nachef N, Lewin S, Selvig D R, Terdiman J P, Mahadevan U, Oh D Y, Fragiadakis G K, Pisco A, Combes A J, Kattah M G2024Nature CommunicationsImmunologyISH, Spatial Analysis, Highplex FL, TMAHALO, HALO AI
N6-methyladenosine-modified circSLCO1B3 promotes intrahepatic cholangiocarcinoma progression via regulating HOXC8 and PD-L1Li J, Xu X, Xu K, Zhou X, Wu K, Yao Y, Liu Z, Chen C, Wang L, Sun Z, Jiao D, Han X2024Journal of Experimental & Clinical Cancer ResearchOncologyArea Quantification, FISH, Highplex FL, TMAHALO
Single-cell resolution characterization of myeloid-derived cell states with implication in cancer outcomeRapozo Guimarães G, Resk Maklouf G, Teixeira C, Santos L, Tessarollo N, Toledo N, Serain A, Lanna C, Pretti M, Vieira da Cruz J, Falchetti M, Dimas M, Filgueiras I, Cabral-Marques O, Ramos R, Macedo F, Rodrigues F, Bastos N, Silva J, Rocha E, Chaves C, Melo A, Moraes-Vieira P, Mori M, Boroni M2024Nature CommunicationsImmuno-oncologyMultiplex IHC, TMAHALO
Single-cell AI-based detection and prognostic and predictive value of DNA mismatch repair deficiency in colorectal cancerNowak M, Jabbar F, Rodewald A-K, Gneo L, Tomasevic T, Harkin A, Iveson T, Saunders M, Kerr R, Oein K, Maka N, Hay J, Edwards J, Tomlinson I, Sansom O, Kelly C, Pezzella F, Kerr D, Easton A, Domingo E, Koelzer V. H, Church D2024Cell Reports MedicineOncologyTMAHALO, HALO AI
NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasisIlter D, Drapela S, Schild T, Ward N, Adhikari E, Low V, Asara J, Oskarsson T, Lau E, DeNicola G, McReynolds M, Gomes A2023Redox BiologyOncology, MetabolismClassifier, Highplex FL, TMAHALO
Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosisBurkard T, Herrero San Juan M, Dreis C, Kiprina A, Namgaladze D, Siebenbrodt K, Luger S, Foerch C, Pfeilschifter J, Weigert A, Radeke H2023Clinical and Translational MedicineOncology, ImmunologyClassifier, Spatial Analysis, Highplex FL, TMAHALO
The CD73 is induced by TGF-β1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanomaGiraulo C, Turiello R, Orlando L, Leonardelli S, Landsberg J, Belvedere R, Rolshoven G, Muller C, Holzel M, Morello S2023Biomedicine & PharmacotherapyOncologyHighplex FL, TMAHALO, HALO AI
ATR-binding lncRNA ScaRNA2 promotes cancer resistance through facilitating efficient DNA end resection during homologous recombination repairChen Y, Shen H, Liu T, Cao K, Wan Z, Du Z, Wang H, Yu Y, Ma S, Lu E, Zhang W, Cai J, Gao F, Yang Y2023Journal of Experimental & Clinical Cancer ResearchOncologyHighplex FL, TMAHALO
Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stabilityBenabdallah NS, Dalal V, Scott RW, Marcous F, Sotiriou A, Kommoss FKF, Pejkovska A, Gaspar L, Wagner L, Sánchez-Rivera FJ, Ta M, Thornton S, Nielsen TO, Underhill TM, Banito A2023Nature Structural & Molecular BiologyOncologyMultiplex IHC, TMAHALO, HALO AI
Vascular-Parenchymal Crosstalk Promotes Lung Fibrosis Through BMPR2 SignalingYanagihara T, Tsubouchi K, Zhou Q, Chong M, Otsubo K, Isshiki T, Schupp J, Sato S, Scallan C, Upagupta C, Revill S, Ayoub A, Chong S, Dvorkin-Gheva A, Kaminski N, Tikkanen J, Keshavjee S, Pare G, Guignabert C, Ask K, Kolb M2023American Journal of Respiratory and Critical Care MedicineFibrosisISH, TMAHALO
Intrinsic BMP inhibitor Gremlin regulates alveolar epithelial type II cell proliferation and differentiationYanagihara T, Zhou Q, Tsubouchi K, Revill S, Ayoub A, Gholiof M, Chong S, Dvorkin-Gheva A, Ask K, Shi W, Kolb M2023Biochemical and Biophysical Research CommunicationsOtherISH, TMAHALO
Targeting the ATF6-mediated ER stress response and autophagy blocks integrin-driven prostate cancer progressionMacke A, Pachikov A, Divita T, Morris M, LaGrange C, Holzapfel M, Kubyshkin A, Zyablitskaya E, Makalish T, Eremenko S, Qiu H, Riethoven J, Hemstreet G, Petrosyan A2023Molecular Cancer ResearchOncologyClassifier, Area Quantification, Multiplex IHC, TMAHALO
FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinomaMorris HT, Bamlet WR, Razidlo GL, Machesky LM2023Pathology – Research and PracticeOncologyCytonuclear, TMAHALO
Positive regulatory loop of platelet-derived growth factor DD-induced STAT3 activation is associated with poor prognosis in advanced urothelial carcinomaAndo K, Kurashina R, Motoi N, Iizuka T, Inoue M, Maruyama R, Mitani K, Takenobu H, Haruta M, Onuki R, Matsuoka Y, Kamijo T, Kageyama Y2023Biochemical and Biophysical Research CommunicationsOncologyMultiplex IHC, TMAHALO
CD15 Is a Risk Predictor and a Novel Target in Clear Cell Renal Cell CarcinomaStenzela PJ, Schindeldeckera MS, Seidmanna L, Herpel E, Hohenfellnere M, Hatiboglue G, Foerscha S, Porubskya S, Macher-Goeppingera S, Rotha W, Tagscherer KE2023PathobiologyOncologyMultiplex IHC, TMAHALO
Nuclear expression of pSTAT3Tyr705 and pSTAT3Ser727 in the stromal compartment of localized hormone-naïve prostate cancerMarginean F, Hellsten R, Krzyanowska A, Bjartell A2022Pathology – Research and PracticeOncologyTMAHALO
Tertiary lymphoid structures critical for prognosis in endometrial cancer patients – a TransPORTEC studyHoreweg N, Workel, H, Loiero D, Church D, Vermij L, Léon-Castillo A, Krog R, de Boer S, Nout R, Powel M, Mileshkin L, MacKay H, Leary A, Singh N, Jürgenliemk-Schulz I, Creutzberg C, Koelzer V, Nijman H, Bosse T, Bruyn M2022Nature CommunicationsOncologyTMAHALO, HALO AI
Type I interferon regulates proteolysis by macrophages to prevent immunopathology following viral infectionLee A, Feng E, Chew M, Balint E, Poznanski S, Giles E, Zhang A, Marzok A, Revill S, Vahedi F, Dubey A, Ayaub E, Jimenez-Saiz R, McGrath J, Ritchie T, Jordana M, Jonigk D, Ackermann M, Ask K, Miller M, Richards C, Ashkar A2022PLOS PathogensInfectious DiseaseArea Quantification, Multiplex IHC, TMAHALO
LncRNA scaRNA2 bridges DNA end-resection to homologous recombination repair mediated chemoradioresistanceYang Y, Chen Y, Shen H, Liu T, Cao K, Wan Z, Du Z, Wang H, Yu Y, Ma S, Li B, Zhang W, Cai J, Gao F2022Research SquareOncologyHighplex FL, TMAHALO
The proteomic landscape of glioblastoma recurrence reveals novel and targetable immunoregulatory driversTatari N, Khan S, Livingstone J, Zhai K, Mckenna D, Ignatchenko V, Chokshi C, Gwynne W, Singh M, Revill S, Mikolajewicz N, Zhu C, Chan J, Hawkins C, Lu J, Provias J, Ask K, Morrissy S, Brown S, Weiss T, Weller M, Han H, Greenspoon J, Moffat J, Venugopal C, Boutros P2022Acta NeuropathologicaOncologyMultiplex IHC, TMAHALO
Developing a Nomogram for Preoperative Prediction of Cervical Cancer Lymph Node Metastasis by Multiplex ImmunofluorescenceWu J, Guo Q, Zhu J, Wu Y, Liang S Chen S, Wang S, Ju X, Wu X2022AuthoreaOncologySpatial Analysis, Highplex FL, TMAHALO
The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signalingZhang B, Liu Q, Wen W, Gao H, Wei W, Tang A, Qin B, Lyu H, Meng X, Li K, Jin H, Yu F, Pan Q Lin J, Lee M2022Cell DiscoveryOncologyTMAHALO
Low CD8 T Cell Counts Predict Benefit from Hypoxia-Modifying Therapy in Muscle-Invasive Bladder CancerSmith V, Mukherjee D, Tsakiroglou A, Baker A, Mistry H, Choudhury A, Hoskin P, Illidge T, West C2022CancersImmuno-oncologyTMAHALO
Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survivalSinghal SK, Byun JS, Park S, Yan T, Yancey R, Caban A, Hernandez SG, Hewitt SM, Boisvert H, Hennek S, Bobrow M, Ahmed SU, White J, Yates C, Aukerman A, Vanguri R, Bareja R, Lenci R, Farré PL, De Siervi A, Nápoles AM, Vohra N, Gardner K2021Communications BiologyOncologyCytonuclear, TMAHALO
Tumor cell PD-L1 expression is a strong predictor of unfavorable prognosis in immune checkpoint therapy-naive clear cell renal cell cancerMöller K, Fraune C, Blessin NC, Lennartz M, Kluth M, Hube-Magg C, Lindhorst L, Dahlem R, Fisch M, Eichenauer T, Riechardt S, Simon R, Sauter G, Büscheck F, Höppner W, Matthies C, Doh O, Krech T, Marx AH, Zecha H, Rink M, Steurer S, Clauditz TS2021International Urology and NephrologyImmuno-oncologyMultiplex IHC, TMAHALO
Prognostic role of proliferating CD8+ cytotoxic Tcells in human cancersBlessin NC, Li W, Mandelkow T, Jansen HL, Yang C, Raedler JB, Simon R, Büscheck F, Dum D, Luebke AM, Hinsch A, Möller K, Menz A, Bernreuther C, Lebok P, Clauditz T, Sauter G, Marx A, Uhlig R, Wilczak W, Minner S, Krech T, Fraune C, Höflmayer D, Burandt E, Steurer S2021Cellular OncologyImmuno-oncologyHighplex FL, TMAHALO
Epigenetic modifications precede molecular alterations and drive human hepatocarcinogenesisCzauderna C, Poplawski A, O’Roourke C, Castven D, Pérez-Aguilar B, Becker D, Heilmann-Heimbach S, Odenthal M, Amer W, Schmiel M, Drebber U, Binder H, Ridder D, Schindeldecker M, Straub B, Galle P, Andersen J, Thorgeirsson S, Park Y, Marquardt J2021JCI InsightOncologyCytonuclear, TMAHALO
Comprehensive clinicopathologic study of alpha fetoprotein-expression in a large cohort of patients with hepatocellular carcinomaRidder D, Weinmann A, Schindeldecker M, Urbansky L, Berndt K, Gerber T, Lang H, Lotz J, Lackner K, Roth W, Straub B2021International Journal of CancerOncologyCytonuclear, TMAHALO
Prognostic image-based quantification of CD8CD103 T cell subsets in high-grade serous ovarian cancer patientsPaijens S, Vledder A, Loiero D, Duiker E, Bart J, Hendriks A, Jalving M, Workel H, Hollema H, Werner N, Plat A, Wisman G, Yigit R, Arts H, Kruse A, de Lange N, Koelzer V, Bruyn M, Nijman H2021OncoImmunologyImmuno-oncologyTMAHALO, HALO AI
The MK2/Hsp27 axis is a major survival mechanism for pancreatic ductal adenocarcinoma under genotoxic stressGrierson P, Dodhiawala P, Cheng Y, Chen T, Khawar I, Wei Q, Zhang D, Li L, Herndon J, Monahan J, Ruzinova M, Lim K2021Science Translational MedicineOncologyArea Quantification, TMAHALO
Prognostic and Predictive Value of Tumor-infiltrating Leukocytes and of Immune Checkpoint Molecules PD1 and PDL1 in Clear Cell Renal Cell CarcinomaStenzel PJ, Schindeldecker M, Teagscherer KE, Foersch S, Herpel E, Hohenfellner M, Hatiboglu G, Alt J, Thomas C, Haferkamp A, Roth W, Macher-Goeppinger S2020Translational OncologyOncology, Immuno-oncologyClassifier, Cytonuclear, TMAHALO
Mechanical regulation of glycolysis via cytoskeleton architecturePark JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G2020NatureOncologyClassifier, Cytonuclear, TMAHALO
Prognostic significance of mesothelin expression in colorectal cancer disclosed by area-specific four-point tissue microarraysShiraishi T, Shinto E, Nearchou IP, Tsuda H, Kajiwara Y, Einama T, Caie PD, Kishi Y, Ueno H2020Vichows ArchivOncologyCytonuclear, TMAHALO, HALO AI
Prevalence of CD8+ cytotoxic lymphocytes in human neoplasmsBlessin NC, Spriestersbach P, Li W, Mandelkow T, Dum D, Simon R, Hube-Magg C, Lutz F, Viehweger F, Lennartz M, Fraune C, Nickelsen V, Fehrle W, Göbel C, Weidemann S, Clauditz T, Lebok P, Möller K, Steurer S, Izbicki JR, Sauter G, Minner S, Jacobsen F, Luebke AM, Büscheck F, Höflmayer D, Wilczak W, Burandt E, Hinsch A2020Cellular OncologyOncology, Immuno-oncologyMembrane, TMAHALO
The prevalence and prognostic significance of estrogen receptor beta expression in non-small cell lung cancerEnwere EK, Dean ML, Li H, D’Silva A, Bebb DG2020Translational Lung Cancer ResearchOncologyCytonuclear, TMAHALO
Linking epigenetic signature and metabolic phenotype in IDH mutant and IDH wildtype diffuse gliomaBuran Y, Filipski K, Bernatz S, Baumgarten P, Roller B, Zinke J, Zeiner PS, Ilina E, Senft C, Ronellenfitsch MW, Plate KH, Bähr O, Hattingen E, Steinbach JP, Mittelbron M, Harter PN2020Neuropathology and Applied NeurobiologyNeuroscienceMultiplex IHC, TMAHALO
Genomic analysis of Vascular Invasion in Hepatocellular Carcinoma (HCC) Reveals Molecular Drivers and Predictive BiomarkersKrishnan MS, Rajan KD A, Park J, Arjunan V, Marques FJG, Bermudez A, Girvan OA, Hoang NS, Yin J, Nguyen MH, Kothary N, Pitteri S, Felsher DW, Dhanasekaran R2020HepatologyOncologyMultiplex IHC, TMAHALO
B7-H3 immune checkpoint expression is a poor prognostic factor in colorectal carcinomaLu Z, Zhao Z-X, Cheng P, Huang F, Guan X, Zhang M-G, Chen H-P, Liu Z, Jiang Z, Zheng Z-X, Zou S-M, Wang X-S2020Modern PathologyImmuno-oncologyMultiplex IHC, TMAHALO
PIK3CA mutation and CNV status and post-chemoradiotherapy survival in patients with cervical cancerMartell K, McIntyre JB, Kornaga EN, Chan AMY, Phan T, Kobel M, Enwere EK, Dean ML, Ghatage P, Lees-Miller SP, Doll CM2020Gynecologic OncologyOncologyClassifier, Highplex FL, TMAHALO
PLX3397 treatment inhibits constitutive CSF1R-induced oncogenic ERK signaling, reduces tumor growth, and metastatic burden in osteosarcomaSmeester BA, Slipek NJ, Pomeroy EJ, Laoharawee K, Osum SH, Larsson AT, Williams KB, Stratton N, Yamamoto K, Peterson JJ, Rathe SK, Mills LJ, Hudson WA, Crosby MR, Wang M, Rahramann EP, Moriarity SB, Largaespada DA2020BoneOncologyCytonuclear, TMAHALO
Prognostic Integrated Image-Based Immune and Molecular Profiling in Early-Stage Endometrial CancerHoreweg N, de Bruyn M, Nout R, Stelloo E, Kedzierska K, Leon-Castillo A, Plat A, Mertz K, Osse M, Jurgenliemk-Schulz I, Lutgens L, Jobsen J, van der Steen-Banasik E, Smit V, Creutzberg C, Bosse T, Nijman H, Koelzer V, Church D2020Cancer Immunology ResearchOncologyTMAHALO, HALO AI
The role of WNT10B in normal prostate gland development and prostate cancerMadueke I, Hu W-Y, Hu D, Swanson SM, Griend DV, Abern M, Prins GS2019The ProstateOncologyMultiplex IHC, TMAHALO, HALO AI

Related HALO Modules

Highplex FL

Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.

Learn More
Multiplex IHC

Quantify expression of up to five brightfield stains in any cellular compartment - membrane, nucleus or cytoplasm.

Learn More
Tissue Classifier Add-on

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

Learn More

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