The Islet Quantification module counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and/or nuclear positivity for up to two stains.
In the first example, the software is configured to identify islets using two cytoplasmic islet-specific stains, red and brown. Neutral cells are marked blue, red stain positive cells are marked red, brown positive cells are marked green and dual positive cells are marked yellow. The islet area is yellow and the boundaries are demarked with a black lines.
In addition to measuring cytoplasmic positivity, this tool can also be configured to detect positivity within islet nuclei as shown in the second example. Here, proliferating beta cells are identified as dual positive for insulin (red stain) and Ki67 (brown nuclear stain). Insulin positive cells are marked red in the cytoplasm and Ki67 positive cells are marked green in the nucleus.
The module reports islet counts, and islet measurements (diameter & area), islet cell counts (beta cells, positive cells, dual positive cells, and negative cells), and a histogram of islet frequency by diameter ranges.
The Islet Quantification module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and file formats.
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