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ISH-IHC Module

Use the HALO® ISH-IHC module and reagents from Molecular Instruments or ACD, a Bio-techne brand, to simultaneously analyze a nuclear stain and up to four IHC biomarkers or ISH probes on a cell-by-cell basis. This module enables in-depth analysis of the corresponding protein and gene expression profile of every cell across a brightfield image, reporting outputs including IHC positive cells in total and by cell compartment, probe copies and area per cell, and calculated H-scores for each probe. Optimize your image analysis with available pre-trained AI-based membrane and/or nuclear segmentation, now included with the HALO platform, and leverage interactive markups to dynamically explore colocalization and combined cell phenotypes.

Try it out! Click here to initiate your free proof-of-concept HALO image analysis.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

RNAscope® Publications with the HALO® Image Analysis Platform

Read this publications list to see examples of research in areas from neuroscience to oncology using HALO to analyze RNAscope images.

Quantitative RNAscope Image Analysis Guide​

Advance your RNAscope image analysis by downloading the latest Quantitative RNAscope Image Analysis Guide from Indica Labs and ACD, a Bio-techne brand.

Publication Spotlight

The table below includes publications that cite the ISH-IHC and FISH-IF modules. 

Your publication not on the list?  Drop us an email to let us know about it!

Title Authors Year Journal Application HALO Modules Product
Detection of EpsteinñBarr Virus in Periodontitis: A Review of Methodological Approaches Tonoyan L, Chevalier M, Vincent-Bungnas S, Marsault R, Doglio A 2021 Mircroorganisms Review ISH/FISH, ISH-IHC/FISH-IF HALO
Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry Wright JH, Huang L-Y, Weaver S, Archila LD, McAfee MS, Hirayama AV, Chapuis AG, Bleakley M, Rongvaux A, Turtle CJ, Chanthaphavong S, Campbell JS, Pierce RH 2020 Journal of Immunological Methods Immunology ISH/FISH, ISH-IHC/FISH-IF HALO
Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders Eroglu E, Yen C, Witman N, Elewa A, Araus A, Wang H, Szattler T, Umeano C, Sohlmer J, Goedel A, Simon A, Chien K 2022 Nature Cell Biology Myology ISH/FISH, ISH-IHC/FISH-IF HALO
Satellite repeat RNA expression in epithelial ovarian cancer associates with a tumor immunosuppressive phenotype Porter R, Sun S, Flores M, Berzolla E, You E, Phillips I, KC N, Desai N, Tai E, Szabolcs A, Lang E, Pankaj A, Raabe M, Thapar V, Xu K, Nieman L, Rabe D, Kolin D, Stover E, Pepin D, Stott S, Deshpande V, Liu J, Solovyov A, Matulonis U, Greenbaum B, Ting D 2022 The Journal of Clinical Investigation Immuno-oncology ISH/FISH, ISH-IHC/FISH-IF HALO
Systemic Nos2 Depletion and Cox inhibition limits TNBC disease progression and alters lymphoid cell spatial orientation and density Somasundaram V, Ridnour L, Cheng R, Walke A, Kedei N, Bhattacharyya D, Wink A, Edmondson E, Butcher D, Warner A, Dorsey T, Scheiblin D, Heinz W, Bryant R, Kinders R, Lipkowitz S, Wong S, Pore M, Hewitt S, McVicar D, Anderson S, Chang J, Glynn S, Ambs S, Lockett S, Wink D 2022 Redox Biology Oncology Classifer, ISH/FISH, Spatial Analysis, Registration, ISH-IHC/FISH-IF HALO
Annexin A2/TLR2/MYD88 pathway induces arginase 1 expression in tumor-associated neutrophils Zhang H, Zhu X, Friesen T, Kwak J, Pisarenko T, Mekvanich S, Velasco M, Randolph T, Kargl J, Houghton A 2022 Journal of Clinical Investigation Immuno-oncology Spatial Analysis, ISH-IHC/FISH-IF HALO
Spatiotemporal transcriptome analysis reveals critical roles for mechano-sensing genes at the border zone in remodeling after myocardial infarction Yamada S, Ko T, Hatsuse S, Nomura S, Zhang B, Dai Z, Inoue S, Kubota M, Sawami K, Yamada T, Sassa T, Katagiri M, Fujita K, Katoh M, Ito M, Harada M, Toko H, Takeda N, Morita H, Aburatani H, Komuro I 2022 Nature Cardiovascular Research Myology ISH-IHC/FISH-IF HALO
Loss of functional System x-c uncouples aberrant postnatal neurogenesis from epileptogenesis in the hippocampus of Kcna1-KO mice Aloi M, Thompson S, Quartapella N, Noebels J 2022 Cell Reports Neuroscience ISH-IHC/FISH-IF HALO
Multiparameter immunohistochemistry analysis of HIV DNA, RNA and immune checkpoints in lymph node tissue Richardson Z, Deleage C, Tutuka C, Walkiewica M, Del Rio-Estrada P, Pascoe R, Evans V, Reyesteran G, Gonzales M, Roberts-Thomson S, Gonzalez-Navarro M, Torres-Ruiz, F, Estes J, Lewin S, Cameron P 2021 Journal of Immunological Methods Infectious Disease ISH-IHC/FISH-IF HALO
The potassium channel auxiliary subunit Kv_2 (Kcnab2) regulates Kv1 channels and dopamine neuron firing Yee J, Rastani A, Soden M 2022 Journal of Neurophysiology Neuroscience ISH-IHC/FISH-IF HALO
Transcriptional vulnerabilities of striatal neurons in human and rodent models of Huntington’s disease Matsushima A, Pineda S, Crittenden J, Lee H, Galani K, Mantero J, Tombaugh G, Kellis M, Heiman M, Graybiel A 2023 Nature Communications Neuroscience Area Quantification, ISH-IHC/FISH-IF HALO
Exploration of spatial heterogeneity of tumor microenvironment in nasopharyngeal carcinoma via transcriptional digital spatial profiling Wang L, Wang D, Zeng X, Zhang Q, Wu H, Liu J, Wang Y, Liu G, Poan Y 2023 International Journal of Biological Sciences Oncology Area Quantification, ISH-IHC/FISH-IF HALO

Related HALO Modules

Tissue Classifier Add-on

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Simultaneously analyze up to three chromogenic and/or silver-labelled DNA or RNA ISH probes on a cell-by-cell basis, measuring spot numbers and area per cell and compartment, and calculated H-scores for each probe.

Learn More

Simultaneously analyze an unlimited number of fluorescent nucleic acid probes on a cell-by-cell basis, measuring spot numbers and area per cell and compartment, and calculated H-scores for each probe.

Learn More

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