The Microglial Activation FL module measures fluorescently-stained microglia cell activation in whole slide images. This module detects microglia, soma, and processes, and ultimately reports the number of activated microglial cells, the number of inactivated microglial cells, and the number of negative (non-microglia) cells detected. In addition to cell classification and counting, this tool precisely measures cell processes. It reports the total process area and length, as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points and end points per cell. An interactive markup image output option enhances visualization of results by selecting which of the following are displayed: activated microglia, inactivated microglia, microglial processes and skeleton, branch points, end points, as well as the microglia radius.
File formats supported by the HALO image analysis platform:
- Non-proprietary (JPG, TIF, OME.TIFF)
- Nikon (ND2)
- 3D Histech (MRXS)
- Akoya (QPTIFF, component TIFF)
- Olympus / Evident (VSI)
- Hamamatsu (NDPI, NDPIS)
- Aperio (SVS, AFI)
- Zeiss (CZI)
- Leica (SCN, LIF)
- Ventana (BIF)
- Philips (iSyntax, i2Syntax)
- KFBIO (KFB, KFBF)
- DICOM (DCM*)
*whole-slide images
Publication Spotlight
The table below includes publications that cite the Microglial Activation module.
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| Title | Authors | Year | Journal | Application | HALO Modules | Product |
|---|---|---|---|---|---|---|
| Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum | Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T | 2018 | Neurobiology of Aging | Neuroscience | Area Quantification, Object Colocalization, Microglia | HALO |
| Diverse human astrocyte and microglial transcriptional responses to Alzheimerís pathology | Smith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P | 2021 | Acta Neuropathologica | Neuroscience | Area Quantification, Multiplex IHC, Microglia | HALO |
| T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of†?-Synuclein Fibrils | George S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P | 2021 | Journal of Parkinson's Disease | Immunology, Neuroscience | Microglia | HALO |
| Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in Dementia | Naseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M | 2022 | Biomolecules | Neuroscience | Microglia | HALO |
| Association between APOE genotype and microglial cell morphology | Kloske C, Gearon M, Weekman E, Rogers C, Patel E, Bachstetter A, Nelson P, Wilcock D | 2023 | Journal of Neuropathology & Experimental Neurology | neuroscience | Object Colocalization, Microglia | HALO |
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Quantify microglial activation based on length and thickness of microglial processes.
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