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HALO® Module

Microglial Activation FL

The Microglial Activation FL module measures fluorescently-stained microglia cell activation in whole slide images. This module detects microglia, soma, and processes, and ultimately reports the number of activated microglial cells, the number of inactivated microglial cells, and the number of negative (non-microglia) cells detected. In addition to cell classification and counting, this tool precisely measures cell processes. It reports the total process area and length, as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points and end points per cell. An interactive markup image output option enhances visualization of results by selecting which of the following are displayed: activated microglia, inactivated microglia, microglial processes and skeleton, branch points, end points, as well as the microglia radius.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Microglial Activation module. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO ModulesProduct
Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T2018Neurobiology of AgingNeuroscienceArea Quantification, Object Colocalization, MicrogliaHALO
Diverse human astrocyte and microglial transcriptional responses to Alzheimerís pathologySmith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P2021Acta NeuropathologicaNeuroscienceArea Quantification, Multiplex IHC, MicrogliaHALO
T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of†?-Synuclein FibrilsGeorge S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P2021Journal of Parkinson's DiseaseImmunology, NeuroscienceMicrogliaHALO
Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in DementiaNaseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M2022BiomoleculesNeuroscienceMicrogliaHALO
Association between APOE genotype and microglial cell morphologyKloske C, Gearon M, Weekman E, Rogers C, Patel E, Bachstetter A, Nelson P, Wilcock D2023Journal of Neuropathology & Experimental NeurologyneuroscienceObject Colocalization, MicrogliaHALO
Characterization of Traumatic Brain Injury in a Gyrencephalic Ferret Model Using the Novel Closed Head Injury Model of Engineered Rotational Acceleration (CHIMERA)Krieg J, Leonard A, Tuner R, Corrigan F2023Neurotrauma ReportsNeuroscienceMicrogliaHALO
Anatomical distribution of Fyn kinase in the human brain in Parkinson's diseaseGuglietti B, Mustafa S, Corrigan F, Collins-Praino LE2023Parkinsonism & Related DisordersNeuroscienceMicrogliaHALO
Female mice display sex-specific differences in cerebrovascular function and subarachnoid haemorrhage-induced injuryDinh DD, Wan H, Lidington D, Bolz SS2024EBioMedicine NeuroscienceMicrogliaHALO

Related HALO Modules

Object Colocalization

Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Object Colocalization FL

Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Microglial Activation

Quantify microglial activation based on length and thickness of microglial processes.

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