The Microglial Activation FL module measures fluorescently-stained microglia cell activation in whole slide images. This module detects microglia, soma, and processes, and ultimately reports the number of activated microglial cells, the number of inactivated microglial cells, and the number of negative (non-microglia) cells detected. In addition to cell classification and counting, this tool precisely measures cell processes. It reports the total process area and length, as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points and end points per cell. An interactive markup image output option enhances visualization of results by selecting which of the following are displayed: activated microglia, inactivated microglia, microglial processes and skeleton, branch points, end points, as well as the microglia radius.
File formats supported by the HALO image analysis platform:
- Non-proprietary (JPG, TIF, OME.TIFF)
- Nikon (ND2)
- 3D Histech (MRXS)
- Akoya (QPTIFF, component TIFF)
- Olympus / Evident (VSI)
- Hamamatsu (NDPI, NDPIS)
- Aperio (SVS, AFI)
- Zeiss (CZI)
- Leica (SCN, LIF)
- Ventana (BIF)
- Philips (iSyntax, i2Syntax)
- KFBIO (KFB, KFBF)
- DICOM (DCM*)
*whole-slide images
Interactive Markup Images: Providing a Dynamic Look at HALO® Analysis Results
At Indica Labs, our HALO platforms are optimized for ease-of-use as well as powerful, accurate analysis, and include multiple features to help users get the
Announcing the launch of a new Microglial Activation module for fluorescence and an updated brightfield module
In this blog post, you can learn about some of the new features in these modules, where to find the user guides, tutorial videos, and
Masterclass Webinar: Neurobiology Image Analysis with HALO and HALO AI
29 September 2022 | Join us for this 1-hour webinar for a live demonstration of neurobiology image analysis using HALO® and HALO AI. Dr. Levi
Publication Spotlight
The table below includes publications that cite the Microglial Activation module.
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| Title | Authors | Year | Journal | Application | HALO Modules | Product |
|---|---|---|---|---|---|---|
| Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum | Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T | 2018 | Neurobiology of Aging | Neuroscience | Area Quantification, Object Colocalization, Microglia | HALO |
| Diverse human astrocyte and microglial transcriptional responses to Alzheimerís pathology | Smith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P | 2021 | Acta Neuropathologica | Neuroscience | Area Quantification, Multiplex IHC, Microglia | HALO |
| T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of†?-Synuclein Fibrils | George S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P | 2021 | Journal of Parkinson's Disease | Immunology, Neuroscience | Microglia | HALO |
| Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in Dementia | Naseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M | 2022 | Biomolecules | Neuroscience | Microglia | HALO |
| Association between APOE genotype and microglial cell morphology | Kloske C, Gearon M, Weekman E, Rogers C, Patel E, Bachstetter A, Nelson P, Wilcock D | 2023 | Journal of Neuropathology & Experimental Neurology | neuroscience | Object Colocalization, Microglia | HALO |
| Characterization of Traumatic Brain Injury in a Gyrencephalic Ferret Model Using the Novel Closed Head Injury Model of Engineered Rotational Acceleration (CHIMERA) | Krieg J, Leonard A, Tuner R, Corrigan F | 2023 | Neurotrauma Reports | Neuroscience | Microglia | HALO |
| Anatomical distribution of Fyn kinase in the human brain in Parkinson's disease | Guglietti B, Mustafa S, Corrigan F, Collins-Praino LE | 2023 | Parkinsonism & Related Disorders | Neuroscience | Microglia | HALO |
| Female mice display sex-specific differences in cerebrovascular function and subarachnoid haemorrhage-induced injury | Dinh DD, Wan H, Lidington D, Bolz SS | 2024 | EBioMedicine | Neuroscience | Microglia | HALO |
| Association between APOE genotype and microglial cell morphology | Kloskey CM, Gearon MD, Weekman EM, Rogers C, Patel E, Bachstetter A, Nelson PT, Wilcock DM | 2023 | Science Advances | Neuroscience | Object Colocalization, Microglia | HALO |
Related HALO Modules
Simultaneously analyze up to five chromogenic stains and measure object density, area, diameter, and optical density, as well as colocalizations, if applicable.
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Simultaneously analyze an unlimited number of fluorescent dyes and measure object density, area, diameter, and intensity, as well as colocalizations, if applicable.
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Quantify microglial activation based on length and thickness of microglial processes.
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