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HALO® Module

Microglial Activation FL

The Microglial Activation FL module measures fluorescently-stained microglia cell activation in whole slide images. This module detects microglia, soma, and processes, and ultimately reports the number of activated microglial cells, the number of inactivated microglial cells, and the number of negative (non-microglia) cells detected. In addition to cell classification and counting, this tool precisely measures cell processes. It reports the total process area and length, as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points and end points per cell. An interactive markup image output option enhances visualization of results by selecting which of the following are displayed: activated microglia, inactivated microglia, microglial processes and skeleton, branch points, end points, as well as the microglia radius.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Microglial Activation module. 

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Title Authors Year Journal Application HALO Modules Product
Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T 2018 Neurobiology of Aging Neuroscience Area Quantification, Object Colocalization, Microglia HALO
Diverse human astrocyte and microglial transcriptional responses to Alzheimerís pathology Smith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P 2021 Acta Neuropathologica Neuroscience Area Quantification, Multiplex IHC, Microglia HALO
T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of†?-Synuclein Fibrils George S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P 2021 Journal of Parkinson's Disease Immunology, Neuroscience Microglia HALO
Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in Dementia Naseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M 2022 Biomolecules Neuroscience Microglia HALO
Association between APOE genotype and microglial cell morphology Kloske C, Gearon M, Weekman E, Rogers C, Patel E, Bachstetter A, Nelson P, Wilcock D 2023 Journal of Neuropathology & Experimental Neurology neuroscience Object Colocalization, Microglia HALO

Related HALO Modules

Object Colocalization FL

Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Object Colocalization

Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

Learn More
Microglial Activation

Quantify microglial activation based on length and thickness of microglial processes.

Learn More

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