Microglial Activation Quantification

All Applications, Brightfield, Cell-Based Analysis, Neuroscience

Microglial Activation

Product Overview

The Microglial Activation module measures microglia cell activation in brightfield whole slide images and photomicrographs. This module detects microglia, soma, and processes, and ultimately reports the number of activated microglial cells, the number of inactivated microglial cells, and the number of negative (non-microglia) cells detected. In addition to cell classification and counting, this tool precisely measures cell processes. It reports the total process area and length, as well as the average process area, length, and thickness, and quantifies process branching by counting the number of branch points per cell.

In the example shown above, the microglia are identified in brown and all nuclei are counterstained with hematoxylin. Processes are identified in yellow and the cell bodies of activated microglia are in red and inactive or resting microglia are in green.

The Microglial Activation module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

Microglial Activation

Here are a few publications that cite use of our Microglial Activation module. Your publication not on the list? Drop us an email to let us know about it!

Title	Authors	Year	Journal	Application	HALO Modules
Immune evasion before tumour invasion in early lung squamous carcinogenesis	Mascaux C, Angelova M, Vasaturo A, Beane J, Hijazi K, Anthoine G, Rothe F, Willard-Gallo K, Haller A, Ninane V, Burny A, Sculier J-P, Spira A, Galon J	2019	Nature	Oncology, Immuno-oncology	Microglia
Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum	Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T	2018	Neurobiology of Aging	Neuroscience	Area Quantification, Object Colocalization, Microglia
Diverse human astrocyte and microglial transcriptional responses to Alzheimer’s pathology	Smith A, Davey K, Tsartsalis S, Khozoie C, Fancy N, Tang S, Liaptsi E, Weinert M, McGarry A, Muirhead R, Gentleman S, Owen D, Matthews P	2021	Acta Neuropathologica	Neuroscience	Area Quantification, Multiplex IHC, Microglia
T Cells Limit Accumulation of Aggregate Pathology Following Intrastriatal Injection of α-Synuclein Fibrils	George S, Tyson T, Rey N, Sheridan R, Peelaerts W, Becker K, Schulz E, Meyerdirk L, Burmeister A, von Linstow C, Steiner J, Galvis M, Ma J, Pospisilik J, Labrie V, Brundin L, Brundin P	2021	Journal of Parkinson's Disease	Immunology, Neuroscience	Microglia
Traumatic Brain Injury Leads to Alterations in Contusional Cortical miRNAs Involved in Dementia	Naseer S, Abelleira-Hervas L, Savani D, de Burgh R, Aleksynas R, Donat C, Syed N, Sastre M	2022	Biomolecules	Neuroscience	Microglia

Click here to initiate your free proof-of-concept HALO® image analysis.

brightfield, cell-based analysis, IHC, neuroscience