Melanoma, a highly aggressive type of skin cancer originating from pigment-producing cells called melanocytes, can be a source of diagnostic confusion for pathologists. While primary
High-grade serous ovarian cancer (HGSC) is known to have a high degree of both genetic and immunologic heterogeneity, can be resistant to chemotherapy, and commonly
SARS-CoV-2 vaccines based on the ancestral Wuhan-Hu-1 Spike (S) protein have been shown to be effective in preventing severe COVID-19, hospitalization, and death. The evolution
Daniel Stone,et al, Molecular Therapy Methods & Clinical Development, 2021
Hepatitis B leads to over 850,000 deaths annually due to complications from chronic infection including cirrhosis and hepatocellular carcinoma. While antiviral drugs successfully reduce viral loads in patients, a cure does not yet exist. This study out of the Jerome laboratory at the Fred Hutchinson Cancer Research Center performed in vivo gene editing utilizing a mouse model for chronic HBV infections and demonstrated a proof-of-concept of safe and effective gene editing of the genomic form responsible for replication and persistence. This study utilized the HALO Link image management platform in conjunction with image acquisition on a Leica Biosystems Aperio VERSA 200 slide scanner for their RNAscope studies of Cas9 and HBV.
CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice Read More »
Courtney M. Williams et al, The FASEB Journal, 2021
Williams and colleagues at Regeneron Pharmaceuticals perform structure/function relationship studies on Fgf15 and FGF19 using site-directed mutagenesis and downstream functional assays in order to understand their distinct functions in a common pathway. Both molecules are therapeutic targets due to involvement in hepatocellular carcinoma and bile acid production. This publication identifies a single cysteine residue is identified that controls dimerization and hepatocyte proliferation. Understanding these molecular pathways may inform future studies on hepatocellular carcinoma while limiting toxicity and induction of hepatocyte proliferation. Immunohistochemistry images were quantified in HALO Link using the Cytonuclear IHC module.
