Islet FL
Count and measure pancreatic islets in fluorescent images and quantify the islet area and cells positive for up to three islet-specific dyes.
Metabolism
Vacuole Quantification
Quantify the area, diameter, perimeter, and number of white spaces per region of interest in brightfield images. Ideally suited for analysis of lipids in brown and white adipose tissue, lipid droplets in liver tissue (steatosis), and alveoli area in lung.
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Islet Quantification
Count and measure pancreatic islets in brightfield images and quantify the islet area and cells positive for up to two islet-specific stains.
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Area Quantification FL
Measure the positive area, average intensity and dye colocalization (where applicable) of an unlimited number of fluorescent dyes.
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Area Quantification
Deconvolve up to five colors in brightfield and measure positive area and average optical density for each stain and stain colocalization (where applicable).
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Monomeric/dimeric forms of Fgf15/FGF19 show differential activity in hepatocyte proliferation and metabolic function
Courtney M. Williams et al, The FASEB Journal, 2021
Williams and colleagues at Regeneron Pharmaceuticals perform structure/function relationship studies on Fgf15 and FGF19 using site-directed mutagenesis and downstream functional assays in order to understand their distinct functions in a common pathway. Both molecules are therapeutic targets due to involvement in hepatocellular carcinoma and bile acid production. This publication identifies a single cysteine residue is identified that controls dimerization and hepatocyte proliferation. Understanding these molecular pathways may inform future studies on hepatocellular carcinoma while limiting toxicity and induction of hepatocyte proliferation. Immunohistochemistry images were quantified in HALO Link using the Cytonuclear IHC module.