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Object Colocalization

OBJECT COLOCALIZATION

 

Product Overview

The Object Colocalization module can be used to count and measure up to two labeled objects in brightfield. The module outputs object number, object density, object inner and outer area, object diameter (min/max/median), and average object optical density. Information regarding object colocalizations can be outputted if applicable.

This module is ideal for quantifying microvessel and/or amyloid plaque density as shown in the slider above. In the first example, xenograft sections are probed with anti-CD34 antibodies to identify vessels (brown). The module identifies the stained vessel walls and connects stained areas within a specified connect distance. Where walls are complete, lumens are identified. In the second slide, beta amyloid plaques are stained red and the area and intensity of staining are quantified in brain sections. In the last example, both vessels (brown) and beta amyloid plaques (purple) are stained in brain sections. Here, we use the same module to quantify the area and density of both objects individually as well as the area of colocalization.

The Object Colocalization module integrates seamlessly into the  HALO® platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

OBJECT COLOCALIZATION

The Object Colocalization module can be used to count and measure up to two labeled objects in brightfield. The module outputs object number, object density, object inner and outer area, object diameter (min/max/median), and average object optical density. Information regarding object colocalizations can be outputted if applicable.

This module is ideal for quantifying microvessel and/or amyloid plaque density as shown in the slider above. In the first example, xenograft sections are probed with anti-CD34 antibodies to identify vessels (brown). The module identifies the stained vessel walls and connects stained areas within a specified connect distance. Where walls are complete, lumens are identified. In the second slide, beta amyloid plaques are stained red and the area and intensity of staining are quantified in brain sections. In the last example, both vessels (brown) and beta amyloid plaques (purple) are stained in brain sections. Here, we use the same module to quantify the area and density of both objects individually as well as the area of colocalization.

Here are a few publications that cite use of the Object Colocalization Brightfield or FL modules.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Aging African green monkeys manifest transcriptional, pathological, and cognitive hallmarks of human Alzheimer's disease Cramer PE, Gentzel RC, Tanis KQ, Vardigan J, Wang Y, Connolly B, Manfre P, Lodge K, Renger JJ, Zerbinatti C, Uslaner JM2017Neurobiology of AgingNeuroscienceObject Colocalization
Resistance to Anti-VEGF Therapy Mediated by Autocrine IL6/STAT3 Signaling and Overcome by IL6 Blockade Eichten A, Su J, Adler AP, Zhang L, Ioffe E, Parveen AA, Yancopoulos GD, Rudge J, Lowy I, Lin HC, MacDonald D, Daly C, Duan X, Thurston G2016Cancer ResearchOncology, Immuno-oncologyCytonuclear, Object Colocalization
Cerebral Protection During MitraClip Implantation: Initial Experience at 2 CentersFrerker C, Schlüter M, Sanchez OD, Reith S, Romero ME, Ladich E, Schröder J, Schmidt T, Kreidel F, Joner M2016JACC: Cardiovascular InterventionsMyologyObject Colocalization
Relationship between mismatch repair immunophenotype and long-term survival in patients with resected periampullary adenocarcinoma Heby M, Lundgren S, Nodin B, Elebro J, Eberhard J, Jirström K2018Journal of Translational MedicineOncology, Immuno-oncologyObject Colocalization
Photoacoustic imaging biomarkers for monitoring biophysical changes during nanobubble-mediated radiation treatmentHysi E, Fadhel MN, Wang Y, Sebastian JA, Giles A, Czarnota GJ, Exner AA, Kolios MC2020PhotoacousticsOtherClassifier, Area Quantification, Object Colocalization
Semi-Automated Digital Image Analysis of Pick's Disease and TDP-3 Proteinopathy Irwin DJ, Byrne MD, McMillan CT, Cooper F, Arnold SE, Lee EB, Van Deerlin VM, Xie SX, Lee VM, Grossman M, Trojanowski JQ2015Journal of Histochemistry & CytochemistryNeuroscienceObject Colocalization, Layer Thickness
Age-related pathology after adenoviral overexpression of the leucine-rich repeat kinase 2 in the mouse striatum Kritzingerab A, Ferger B, Gillardon F, Stierstorfer B, Birk G, Kochanek S, Ciossek T2018Neurobiology of AgingNeuroscienceArea Quantification, Object Colocalization, Microglia
The Prognostic Impact of NK/NKT Cell Density in Periampullary Adenocarcinoma Differs by Morphological Type and Adjuvant Treatment Lundgren S, Warfvinge CF, Elebro J, Heby M, Nodin B, Krzyzanowska A, Bjartell A, Leandersson K, Eberhard J, Jirstrom K2016PLOS ONEOncology, Immuno-oncologyObject Colocalization
Pharmacogenetic neuronal stimulation increases human tau pathology and trans-synaptic spread of tau to distal brain regions in mice Schultz MK, Gentzel R, Usenovic M, Gretzul C, Ware C, Parmentier-Batteur S, Schachter JB, Zariwala HA2018Neurobiology of DiseaseNeuroscienceObject Colocalization
Comparison of Biologic Effect and Particulate Embolization after Femoral Artery Treatment with Three Drug-Coated Balloons in Healthy Swine Model Torii S, Jinnouchi H, Sakamoto A, Romero ME, Kolodgie FD, Virmani R, Finn AV2018Journal of Vascular and Interventional RadiologyOtherObject Colocalization
Chronic Verubecestat Treatment Suppresses Amyloid Accumulation in Advanced Aged Tg2576-AβPPswe Mice Without Inducing Microhemorrhage Villarreal S, Zhao F, Hyde LA, Holder D, Forest T, Sondey M, Chen X, Sur C, Parker EM, Kennedy ME2017Journal of Alzheime's DiseaseNeuroscienceObject Colocalization
The integrative clinical impact of tumor-infiltrating T lymphocytes and NK cells in relation to B lymphocyte and plasma cell density in esophageal and gastric adenocarcinomaSvensson MC, Warfvinge CF, Fristedt R, Hedner C, Borg D, Eberhard J, Micke P, Nodin B, Leandersson K, Jirstrom K2017OncotargetImmuno-oncologyObject Colocalization
Automated macrophage counting in DLBCL tissue samples: a ROF filter based approachWagner M, Hansel R, Reinke S, Richter J, Altenbuchinger M, Braumann U-D, Spang R, Loffler Mk Klapper W2019Biological Proectures OnlineOncologyObject Colocalization
Engineered red blood cells as an off-the-shelf allogeneic anti-tumor therapeutic Zhang XZhang X, Lou M, Dastagir SR, Nixon M, Khamhoung A, Schmidt A, Lee A, Subbiah N, McLaughlin DC, Moore CL, Gribble M, Bayhi N, Amin V, Pepi R, Pawar S, Lyford TJ, Soman V, Mellen J, Carpenter CL, Turka LA, Wickham Tj, Chen TF2021Nature CommunicationsOncologyObject Colocalization, Highplex FL
Mitochondria exert age-divergent effects on recovery from spinal cord injuryStewart A, McFarlane K, Vekaria H, Bailey W, Slone S, Tranthem L, Zhang B, Patel S, Sullivan P, Gensel J2021Experimental Neurology
Neuroscience
Object Colocalization
Acute brain inflammation, white matter oxidative stress, and myelin deficiency in a model of neonatal intraventricular hemorrhageGoulding DS, Vogel RC, Gensel JC, Morganti JM, Stromberg AJ, Miller BA2020Journal of NeuroscienceImmunology, NeuroscienceArea Quantification, Object Colocalization
Promyelocytic leukemia protein promotes the phenotypic switch of smooth muscle cells in atherosclerotic plaques of human coronary arteriesKarle W, Becker S, Stenzel P, Knosalla C, Siegel G, Baum O, Zakrzewicz A, Berkholz J2021Clinical ScienceMyologyArea Quantification, Object Colocalization
The GBA1 D409V mutation exacerbates synuclein pathology to differing extents in two alpha-synuclein modelsPolinski N, Martinez T, Ramboz S, Sasner M, Herberth M, Switzer R, Ahmad S, Pelligrino L, Clark S, Marcus J, Smith S, Dave K, Frasier M2022Disease Models & MechanismsNeuroscienceObject Colocalization
Intraoperative abobotulinumtoxinA alleviates pain after surgery and improves general wellness in a translational animal modelCornet S, Carre D, Limana L, Castel D, Meilin S, Horn R, Pons L, Evans S, Lezmi S, Kalinichev M2022Research SquareOtherArea Quantification, Object Colocalization
Combined Blockade of GARP:TGF-β1 and PD-1 Increases Infiltration of T Cells and Density of Pericyte-Covered GARP+ Blood Vessels in Mouse MC38 Tumors Bertrand C, Van Meerbeeck P, de Streel G, Vaherto-Bleeckx N, Benhaddi F, Rouaud L, Noel A, Coulie P, van Baren N, Lucas S2022Frontiers of Immunology
Immuno-oncology
Cytonuclear, Object Colocalization

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