The Object Colocalization module can be used to count and measure up to two labeled objects in brightfield. The module outputs object number, object density, object inner and outer area, object diameter (min/max/median), and average object optical density. Information regarding object colocalizations can be outputted if applicable.
This module is ideal for quantifying microvessel and/or amyloid plaque density as shown in the slider above. In the first example, xenograft sections are probed with anti-CD34 antibodies to identify vessels (brown). The module identifies the stained vessel walls and connects stained areas within a specified connect distance. Where walls are complete, lumens are identified. In the second slide, beta amyloid plaques are stained red and the area and intensity of staining are quantified in brain sections. In the last example, both vessels (brown) and beta amyloid plaques (purple) are stained in brain sections. Here, we use the same module to quantify the area and density of both objects individually as well as the area of colocalization.