Danielle Guerrero

Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters

Area Quantification, COVID-19, HALO Publications, Infectious Disease, Multiplex IHC, Publications

Lisa H Tostanoski, et al, Nature Medicine, 2020.
In this study led by researchers at Harvard Medical School, they report the first demonstration of prevention of severe clinical disease in a hamster model of SARS-CoV-2 that were provided with a single immunization of the adenovirus serotype 26 (Ad26) vaccine. The Ad26 vaccine utilizes a stabilized spike protein of the SARS-CoV-2 and in the United States this vaccine is commonly known as the Johnson and Johnson vaccine. This study, published in print in November 2020 contributed to the scientific body of evidence in animal models that enabled clinical vaccine trials that in turn led to emergency use approvals in the United States, Canada, and other countries. Researchers leveraged the Multiplex IHC module of HALO for evaluation of the percentage of SAR-N protein positive cells and for Iba-1 quantification. The Area Quantification IHC module was used to determine the percentage of SARS-CoV-2 sense or anti-sense probe or Mx1 protein as a function of area. The Cytonuclear IHC module and Area Quantification IHC modules were used in detection of MPO+ or CD3+ cells expressed as a proportion of total alveolar tissue.

Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters Read More »

Two distinct immunopathological profiles in autopsy lungs of COVID-19

HALO AI Publications, Publications

Ronny Nienhold, et al, Nature Communications, 2020
An international consortium of researchers characterized lung tissue from patients with COVID-19 using transcriptomic, histologic, and cellular analyses. Nienhold and colleagues report two phenotypes associated with lethal COVID-19 disease. One showed high levels of interferon stimulated genes in the lungs as well as limited lung damage and high levels of cytokines and viral loads. The second phenotype included severe lung damage with low levels of interferon stimulated genes, low viral loads, and high levels of CD8+ T cells and macrophages. As patients with the first phenotype die sooner, this highlights the need for biomarkers to classify COVID-19 patients and potentially guide treatment. HALO AI was trained using annotations from a pathologist to identify lung tissue and the resulting output was confirmed by pathology review. HALO was also used for quantification of immunohistochemistry analysis of CD3, CD4, CD8, CD20, CD68, CD123, CD163, and PD1.

Two distinct immunopathological profiles in autopsy lungs of COVID-19 Read More »

Press Release: Indica Labs announces launch of enterprise-wide, cloud-based digital pathology deployment at NCI

Blogs, HALO, HALO, HALO AI, News, Press Releases

Albuquerque, NM – October 8, 2020 – Indica Labs, a leading provider of computational pathology software and services, are pleased to announce the formal launch of an enterprise-wide, cloud-based deployment of Indica Labs’ software within the National Cancer Institute (NCI), including HALO®, HALO AI, HALO Link and HALO AP. Accessible to hundreds of users within the NCI, managing millions of digital images, and facilitating the analysis of thousands of images daily, this is the largest single deployment of Indica Labs’ software world-wide.

Press Release: Indica Labs announces launch of enterprise-wide, cloud-based digital pathology deployment at NCI Read More »

Pulmonary Mycobacterium tuberculosis control associates with CXCR3- and CCR6-expressing antigen-specific Th1 and Th17 cell recruitment

HALO AI Publications, Publications

Uma Shanmugasundaram, et al, JCI Insight, 2020
Shanmugasundaram and colleagues used a nonhuman primate model to study T-cell responses associated with latent tuberculosis infection (LTBI). LTBI patients are asymptomatic and are thought to have contained the M. tuberculosis bacteria within granulomatous lesions in the lung. This research group wanted to characterize the immune responses associated with LTBI in order to understand how to prevent the progression to active tuberculosis (TB). In this JCI Insight publication, researchers report that rhesus macaques with LTBI have high levels of M. tuberculosis-specific T cells that produce both IFN- and IL-17. These T cells also express both CXCR3 and CCR6. HALO AI was used to identify percentage of lung tissue composed of granulomas. In addition, the density of CD4+CXCR3+ cells in lung tissue of non-human primate was determined with HALO image analysis. The Tissue Classifier Add-on and the Highplex FL module of HALO were used for quantification of CD4+, CXCR3+, CD68+CD163+, and CD4+CXCR3+ from immunofluorescence microscopy images.

Pulmonary Mycobacterium tuberculosis control associates with CXCR3- and CCR6-expressing antigen-specific Th1 and Th17 cell recruitment Read More »

HALO® MASTERCLASS WEBINAR SERIES

Events, GraphQL API, HALO, HALO AI, HALO Link, HALO Modules, Masterclasses, Muscle Fiber FL module, Muscle Fiber module, Past Webinars, Webinars / , , , , , ,

Fall/Winter of 2020 | Indica Labs is excited to continue our HALO® Masterclass Webinar Series this autumn. Each masterclass webinar will offer a deep dive into a specific module or capability within our HALO, HALO AI, HALO Link or HALO AP platforms, presented by our expert team of application scientists who train and support our customer base worldwide.

HALO® MASTERCLASS WEBINAR SERIES Read More »

High Dimensional Spatial Biology: from Discovery to High Throughput Studies

Events, HALO, HALO Modules, Highplex FL, News, Past Webinars, Spatial Analysis, Webinars / , , ,

15 September 2020 | 7:00 – 9:00 PDT | 10:00 – 12:00 EDT | 15:00 – 17:00 BST
In this webinar, you’ll learn about the importance of multiplex biomarker analysis including how advances in multiplexed immunofluorescent imaging and analysis can reveal biological and spatial information.

High Dimensional Spatial Biology: from Discovery to High Throughput Studies Read More »

Press Release: Indica Labs named a 2020 Top Workplace by The Albuquerque Journal

News, Press Releases

Albuquerque, NM – August 6, 2020 – Indica Labs, a leading provider of computational pathology software, is pleased to announce that they have been awarded a 2020 Top Workplaces™ honor by The Albuquerque Journal.  Indica Labs was the highest ranking company in the life sciences sector to be included in The Journal’s published list of Top Workplaces this year.

Press Release: Indica Labs named a 2020 Top Workplace by The Albuquerque Journal Read More »

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