HALO Tag

Indica Labs / Posts tagged "HALO"

Masterclass Webinar 2: Advanced Techniques and Workflows for Multiplex IHC Analysis using HALO® and HALO AI

25 May 2023 | Following up on our Masterclass Webinar 1 for Multiplex IHC analysis, Masterclass 2 will discuss advanced workflows to help users tackle more challenging brightfield multiplex IHC assays. First, we will demonstrate how advanced cell segmentation and phenotyping options using HALO AI can be combined with the multiplex IHC module to improve image analysis. Two alternative approaches for analyzing multiplex IHC will also be demonstrated including a serial stain workflow to convert brightfield images into...

Getting Started with RNAscope™ Image Analysis in HALO®

28 March 2023 | Join us for this 1-hour webinar to see a live demonstration of RNAscope image analysis using the HALO® platform from Indica Labs. This webinar will demonstrate the basic functionality of HALO’s RNAscope modules for analysis of brightfield and fluorescence images and will discuss available analysis outputs. In addition, select HALO modules that are commonly used with RNAscope image analysis will be demonstrated, and a brief introduction to how HALO AI can advance your RNAscope will be...

Optimizing quantitative analysis of highly multiplexed CODEX images in HALO

31 March 2022 | In this webinar, Dr. Noemi Kedei, a staff scientist at the Collaborative Protein Technology Resource core of the Center for Cancer Research (CCR) at the National Cancer Institute, will present a comprehensive CODEX workflow, from image acquisition to image processing and HALO image analysis. Dr. Kedei leads the core facility that provides this technology as service to CCR investigators, including project consultation and design, antibody panel customization, tissue staining and imaging, image processing, and...

Using digital pathology to determine the role of tumor-infiltrating B cells in clinical outcome of patients with melanoma

15 December 2021 | This webinar will share an analysis of biomarkers using pre-treatment melanoma samples from the dabrafenib plus trametinib arm of the randomized, Phase III COMBI-v trial using the HALO® image analysis platform combined with gene expression analysis using the Nanostring nCounter technology....

THYROID MALT LYMPHOMA:  SELF-HARM TO GAIN POTENTIAL T-CELL HELP

28 October 2021 | The development of extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) is driven by chronic inflammatory responses and acquired genetic changes.  To investigate its genetic bases, we performed targeted sequencing of 93 genes in 131 MALT lymphomas including 76 from the thyroid. We found frequent deleterious mutations of TET2 (86%), CD274 (53%), TNFRSF14 (53%) and TNFAIP3 (30%) in thyroid MALT lymphoma....

Automated detection and quantitation of gastric immune cells in a mouse model of Helicobacter pylori­-driven preneoplastic progression

21 October 2021 | Gastric cancer is the fourth-leading cause of cancer death worldwide. About 80% of cases are attributable to infection with the bacterium Helicobacter pylori (Hp). Untreated Hp infection causes lifelong stomach inflammation that does not eradicate the bacterium. In some individuals, stomach inflammation leads to preneoplastic progression, a series of sequential tissue changes including loss of gastric acid-producing parietal cells, metaplasia, dysplasia, and finally cancer....

CD38 in the Prostate Tumor Microenvironment

6 October 2021 | CD38, a druggable ectoenzyme, is involved in the generation of adenosine, which is implicated in tumor immune evasion. Its expression and role in prostate tumor-infiltrating immune cells (TIICs) have not been elucidated. We characterized CD38 expression on prostate cancer (PC) epithelial cells and TIICs and explored the association of this expression with clinical outcomes. RNAseq from 159 patients with metastatic castration-resistant prostate cancer (mCRPC) in the International Stand Up To Cancer/Prostate Cancer Foundation (SU2C/PCF) cohort and 171...

Automated Detection and Gleason Grading of Prostate Cancer in Digital Pathology

8 September 2021 | Digital pathology provides an advantage of automatizing many routine pathological tasks via computational algorithms and tools. The reporting of prostate cases is time-consuming due to a large number of slides per case. Moreover, tumor aggressivity grading (Gleason score) is well known for its subjective nature and high levels of interobserver variability. In this webinar, we will demonstrate a deep learning-based tool developed for the detection of prostate cancer in histological slides (prostate biopsies and prostatectomy...

Approaches to Analyzing Skin Tissue using HALO and HALO AI

6 May 2021 | Application Scientist, Natasha Carmell, will discuss and demonstrate workflow approaches for analysing skin sections stained with H&E and immunofluorescence. Using HALO AI we will identify and segment the corneal layer, epidermis, dermis, cartilage, sebaceous glands and lesions in H&E murine auricular tissues, followed by the segmentation of carcinoma from melanoma in fluorescent multiplexed Melanoma tissue. This AI based tissue segmentation will then be combined with biomarker quantification to identify CD8, CD68 and PDL1 positive populations using...

Tips & Tricks: Batch Processing Productivity Tools

29 April 2021 | Batch processing workflows can be a significant time saver for HALO image analysis. In this masterclass, US Application Scientist Michael Tomac will cover six different batch process workflows available in HALO, including: - Registration, including batch registration of TMAs - Annotation Editing & Deletion - Analysis - Edit Result Layers - Spatial Analysis from scratch and using saved settings - View Settings - Export Michael will also highlight changes in batch workflows introduced in HALO 3.3, so this is a great opportunity for HALO...

Comparison of stain co-localization in IHC sequential cuts and true co-expression in mIF images from Polaris

8 April 2021 | The webinar will cover a workflow for performing stain co-localization analysis in sequential IHC cuts through color deconvolution, slide registration, and cell-type classification, including export using registered coordinates. The resulting co-localization output will be compared and contrasted to true co-expression on a single slide using mIF techniques (Polaris)....

HALO Image Analysis Masterclass Series: Indica Labs Tech Toolbox Webinar

1 April 2021 | The Indica Labs software products have a number of additional tools to extend the utility of our products beyond the HALO user interface most users are familiar with. These tools allow users to query the underlying database for metadata imported to HALO Link, integrate with LIMS, plugin convolutional neural networks not available in HALO, write analysis modules from scratch, and more....

Webinar: Using the HALO image analysis platform to study pancreas pathology and insulitis in young people with recent-onset Type 1 diabetes

4 March 2021 8:00 AM – 9:00 AM PST | 11:00 AM - 12:00 PM EST | 4:00 PM - 5:00 PM GMT Type 1 diabetes is an autoimmune condition leading to the T-cell mediated destruction of insulin-containing beta cells. Worldwide, fewer than 600 Type 1 diabetes pancreata have been described in the literature or are accessible within tissue biobanks. Due to welcome improvements in the diagnosis and clinical management of Type 1 diabetes, deaths close to diagnosis are now very rare in young...

HALO Image Analysis Masterclass Series: February-March 2021

February 2021 | Indica Labs is excited to continue our HALO® Masterclass Webinar Series this winter. Each masterclass webinar will offer a deep dive into a specific module or capability within our HALO, HALO AI, HALO Link or HALO AP platforms, presented by our expert team of application scientists who train and support our customer base worldwide.These webinars are suitable for prospective customers who want to see a more in depth demonstration as well as current users looking for refresher...

Webinar | HALO® Image Analysis Masterclass Series: Object-based Analysis

28 January 2021 8:00 AM – 9:00 AM PST | 11:00 AM - 12:00 PM EST | 4:00 PM - 5:00 PM GMT | Looking for an easy way to quantify plaques or vessels in your images? Register for our next masterclass! In the first of our 2021 Masterclass series, East Coast Application Scientist Alyssa Myers will discuss object-based analysis. Using HALO, this masterclass will provide an in-depth look at our brightfield and fluorescent object colocalization analysis modules....

Webinar | Digital Pathology in the New Normal: Leveraging HALO® to Investigate a Global Pandemic

21 January 2021 8:00 AM – 9:00 AM PST | 11:00 AM - 12:00 PM EST | 4:00 PM - 5:00 PM GMT | As the world was shutting down in the face of the COVID-19 pandemic, Digital Pathology was thrust to the forefront as a vital option for not only completing existing projects, but more importantly in understanding the disease of COVID-19. Characterizing the mechanisms by which the novel coronavirus attacks the human body, and also understanding the immune system’s response to the...

Digital Pathology in the New Normal: Leveraging HALO to Investigate a Global Pandemic

26 October 2020 11:00 – 12:35 PT As the world was shutting down in the face of the COVID-19 pandemic, Digital Pathology was thrust to the forefront as a vital option for not only completing existing projects, but more importantly in understanding the disease of COVID-19. Characterizing the mechanisms by which the novel coronavirus attacks the human body, and also understanding the immune system’s response to the disease are critical to guiding treatment and influencing outcomes. Researchers around the world utilized...

HALO® MASTERCLASS WEBINAR SERIES

Indica Labs is excited to continue our HALO® Masterclass Webinar Series this autumn. Each masterclass webinar will offer a deep dive into a specific module or capability within our HALO, HALO AI, HALO Link or HALO AP platforms, presented by our expert team of application scientists who train and support our customer base worldwide. These webinars are suitable for prospective customers who want to see a more in depth demonstration as well as current users looking for refresher training or additional...

INDICA LABS’ PHARMA SERVICES: FACILITATING TISSUE BASED IMMUNO-ONCOLOGY RESEARCH

15 October 2020, 9:30 PDT | 12:30 EDT | 17:30 BST In this 30 minute presentation, Jeff Dzubay, Ph.D. will present on the following topics:  - Immuno-Oncology biomarker assays benefit from high-plex technologies to better understand the immune landscape. - There are multiple workflows for multiplex IHC/IF assays. - HALO® is a comprehensive, easy-to-use, image analysis platform for analyzing multiplex assays to understand the immune profile within the spatial context of the tumor microenvironment. - The Pharma Services group at Indica Labs uses the HALO®...

WEBINAR: Bench-to-Bedside Transition of Novel HALO® and HALO AI Assays into Clinical Trials with HALO AP

September 30, 2020 at 8:00 PDT | 11:00 EDT | 16:00 BST Transitioning novel assays underpinned by quantitative image analysis to a clinical environment may seem a daunting task. Whereas research and discovery image analysis workflows are more flexible and open, clinical image analysis assays must be locked down. ...

WEBINAR: IT’S ALL ABOUT LOCATION: NOVEL PROGNOSTIC MODELS DERIVED FROM SPATIAL ANALYSIS OF THE TUMOR-IMMUNE INTERFACE

29 July 2020, 8:00 PDT | 11:00 EDT | 16:00 BST - Biological interactions between functionally distinct immune and tumor cell populations can either promote or inhibit tumor growth. In this 75-minute webinar, we will hear from two research teams who have identified novel prognostic signatures using different spatial analysis and machine learning approaches to measure tumor-immune cell interactions within the tumor immune microenvironment (TIME)....

Virtual US User Group Meeting

In lieu of our scheduled onsite user group meetings, Indica Labs is pleased to announce our 2020 US Virtual HALO User Group Meeting.  To make participation easier for our US audience, we will break down the user groups into four one-hour webinars over three days starting at 9 AM PDT/12 PM EDT. To get things started each day, Indica Labs' applications & pharma services teams will introduce and demonstrate new features and capabilities that are part of our upcoming HALO 3.1, HALO AI, HALO Link and HALO AP...

Integration Between Artificial Intelligence and Digital Pathology at AstraZeneca – Broadcast 1, Upcoming Webinar Hosted by Xtalks

In this free webinar, attendees will learn about how AI-guided technologies can be used to quantify digital pathology images. Presenters will discuss different deployment models for AI in pathology and how to identify projects that will benefit from AI. Four practical-use cases for AI in drug discovery and safety assessment will also be presented....

HALO® IMAGE ANALYSIS MASTERCLASS WEBINAR SERIES 2020

Indica Labs is excited to introduce our new HALO® Masterclass Webinar Series. Each masterclass webinar will offer a deep dive into a specific module or capability within our HALO, HALO AI, HALO Link or HALO AP platforms, presented by our expert team of application scientists who train and support our customer base worldwide. These webinars are suitable for prospective customers who want to see a more in depth demonstration as well as current users looking for refresher training or additional tips...

The Use of Image Fusing in the Deployment of a 7-plex Immunofluorescent Assay

In lieu of our participation in the now cancelled 2020 AACR Conference, Indica Labs are pleased to bring you webinar presentations from two of our esteemed customers in oncology research and drug discovery, David Krull from GlaxoSmithKline and Dr. David Reiss from Bristol Meyers Squibb, who will present on April 27th and 28th, respectively.   Please join us to hear about how Indica Labs image analysis software and pharma services are being used within their organizations to advance oncology drug development....

Spatial Organization of Immune Cells in the Pancreatic Ductal Adenocarcinoma (PDAC) Tumor Microenvironment

In lieu of our participation in the now cancelled 2020 AACR Conference, Indica Labs are pleased to bring you webinar presentations from two of our esteemed customers in oncology research and drug discovery, David Krull from GlaxoSmithKline and Dr. David Reiss from Bristol Meyers Squibb, who will present on April 27th and 28th, respectively.   Please join us to hear about how Indica Labs image analysis software and pharma services are being used within their organizations to advance oncology drug development....

Managing Digital Pathology Workflow in a Busy Histopathology Core Facility using HALO and HALO Link

Core laboratories provide a vital service to researchers by offering expert advice on advanced technologies, shared access to specialized instrumentation and software, as well support for specific types of assays. Increasingly, digital slide scanners and quantitative analysis platforms are being centralized into these core labs to give users access to advanced technologies for both fluorescent and brightfield digital pathology....

Brightplex: Combining the power of multiplexing & the ease of chromogenic IHC to assess complex immune phenotypes in the TME

In this webinar, Dr Anna Martirosyan and Dr. Emmanuel Prestat will introduce the Brightplex platform, including a description of the MDSC Panel, the workflow and techniques employed. A discussion on how the HALO(R) image analysis platform is integrated as an image processing component of the workflow, and how the technology can be applied to investigate the immune contexture of tumors will follow....

Combined analysis of tumor buds, lymphocytic infiltration and their spatial relationship within the tumor microenvironment improves prognostic accuracy colorectal cancer

In this webinar, Ines Nearchou from the University of St. Andrews will discuss the methodology and the results of the study which were recently published in Cancer Immunology Research.  By way of introduction, Dr. Kate Lillard Tunstall, CSO at Indica Labs, will highlight briefly some of the HALO image analysis tools which were used in the study....

Integration of Artificial Intelligence with Digital Pathology: Deployment Considerations & Cases Studies in Pharma

By helping scientists make data-driven decisions faster and more efficiently, artificial intelligence (AI) is revolutionizing drug discovery and development.  Pathology is no exception and the Pathology Sciences team at AstraZeneca in Cambridge UK is leading the charge.  Here, neural networks are being deployed to search digital slides for specific tissue classes, features and pathologies that are not easily quantified using traditional image analysis or are too time-consuming for pathologists to quantify by eye. ...

Transforming tissue-based translational research with advanced multiplexing technologies, image analysis and cloud collaboration

With its high spatial heterogeneity and biological complexity, the tumor immune microenvironment presents a characterization challenge for translational researchers. Conventional immunohistochemistry (IHC) methods only provide insight into a limited number of markers of interest. In this 60-minute webinar, Dr. Sean Downing from Ultivue will explain how they are overcoming these limitations and extracting meaningful, highly multiplexed biomarker data from whole slide images using Ultivue’s proprietary multiplexing technology, UltiMapper™ I/O, in combination with the HALO® image analysis platform. ...

HALO® Analysis Applications in Neuroscience Research

In this one hour webinar, Indica Labs’ Application Scientist, Alyssa Myers, will discuss how the HALO platform can be used for quantitative analysis of whole slide images relevant to neuroscience researchers. Following this introduction, Alyssa will give a real-time demonstration of some of our more popular neuroscience-specific modules, including both brightfield and fluorescence examples....

Ultivue Webinar Features HALO Image Analysis: End-to-End Application of UltiMapper Kits for Multiplexed Immuno-Profiling in FFPE Tumor Samples

In this webinar,  the Ultivue technology and UltiMapper™ I/O kits will be introduced including a fully optimized, automated and optimized staining protocol on the Leica BOND RX platform.  Avoiding any linear unmixing after image acquisition, the UltiMapper I/O whole slide images are directly analyzed using the then processed on Indica Labs HALO software, to derive cell counts, marker co-localization and proximity data analyses. HALO therefore provides the analytical tools to tap into convoluted biomarker signatures and answer complex biological questions...

Computational pathology: A new platform to empower personalized immunotherapy

This webinar is a “must see” for anyone interested in PD-1/PD-L1 targeted immunotherapy and quantitative methods for biomarker assessment in immuno-oncology. In this webinar, Dr. Viktor Koelzer from the Institute of Cancer and Genomic Science at the University of Birmingham and the Wellcome Trust Centre for Human Genetics at the University of Oxford will discuss these critical areas of improvement within the context of two recent studies published by the presenter and his colleagues....

RNAscope® ISH Quantification using the HALO® Image Analysis Platform

In this 60-minute webinar, Indica Labs is pleased to welcome two guest speakers who will discuss how they are using the RNAscope® assay with HALO® quantification software in their institutions. Dr. Gordon Jiang from Beth Israel Deaconess Medical Center | Harvard Medical School will present his original research including the use of chromogenic RNAscope and HALO to analyze PNPLA3 transcirption in Non-Alchoholic Liver Disease (NAFLD). Dr. Julia Jones from Cancer Research UK | Cambridge Institute will explain the RNAscope technology...