Object Based Analysis

Branch Structure FL

Analyze branch-like structures such as retinal vessels or cortical neurons in fluorescence.

Branch Structure

Quantify branched objects such as capillaries in brightfield.

Islet FL

Count pancreatic islets fluorescently-labeled with up to three dyes, quantify number of dye positive cells per islet and area of dye positivity per islet.

Object Colocalization

Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

Vacuole Quantification

Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.

Object Colocalization FL

Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

Islet Quantification

Count pancreatic islets, quantify number of alpha and beta cells in each islet, and determine area of islets stained with insulin and glucagon or other islet-specific stains

Spatial Analysis

Plot cells and objects from one or more images and perform nearest neighbor analysis, proximity analysis, and tumor infiltration analysis.

Tissue Classifier Add-On

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.