HALO Prostate AI
HALO Prostate AI, from Indica Labs, is a CE-IVD certified deep learning based screening tool designed to assist pathologists in the identification and grading of prostate cancer in core needle biopsies. ...
Oncology
Mechanical regulation of glycolysis via cytoskeleton architecture
Jin Suk Park,et al, Nature, 2020
Park and colleagues explored the question of how regulation of glycolysis responds to structural changes in tissue architecture and chose lung cells and tissue for their studies due to the regular mechanical stress experienced during respiration. In vitro studies demonstrated downregulation of glycolysis following a change in substrate via the degradation of the platelet isoform of the phosphofructokinase (PFKP) enzyme. In vitro studies also showed that oncogenic transformation changed the ability of PFKP...
Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival
Sandeep K Singhal, et al, Communications Biology, 2021
Singhal and colleagues apply quantitative automated image analysis to investigate the role of a transcriptional regulator, Kaiso, in a diverse cohort of breast cancer tumors. Specifically, they utilized the Highplex FL Module with the Tissue Microarray Module of HALO to characterize the tumor microenvironment in breast cancer TMA cores, including pan-cytokeratin, PD-L1, CD8, and CD68. They found that cytoplasmic Kaiso is associated with an immune-suppressed tumor microenvironment and found novel connections between Kaiso...
Implementation of PD-L1 22C3 IHC pharmDxTM in Cell Block Preparations of Lung Cancer: Concordance with Surgical Resections and Technical Validation of CytoLyt® Prefixation
Si Kei Lou, et al, Acta Cytologica, 2020
In patients with advanced stage lung disease, it is beneficial to evaluate candidacy for immunotherapy without invasive biopsy testing. Lou et al performed a concordance study to evaluate formalin fixed cell blocks compared to lung tumor resections using a PD-L1 22C3 IHC pharmDx™ assay and found strong concordance between pathologists and HALO image analysis software. Future research will focus on clinical validation by assessing the clinical benefit from immunotherapy following PD-L1 immunohistochemistry on...
FISH-IF Quantification
The ISH Quantification module measures one or two chromogen and/or silver-labeled DNA or RNA ISH probes on a per cell basis and is ideally suited for brightfield single or dual probe RNAscope assays....
ISH-IHC Quantification
The ISH Quantification module measures one or two chromogen and/or silver-labeled DNA or RNA ISH probes on a per cell basis and is ideally suited for brightfield single or dual probe RNAscope assays....
Object Colocalization
The Object Colocalization module is used to count and measure one or two labeled objects, such as microvessels and plaques, in brightfield histology slides....
Multiplex IHC
The Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane)....
Highplex FL
The High-Plex FL module allows for simultaneous analysis of up to 8 immunofluorescently-labeled markers in any cellular compartment - nucleus, cytoplasm, and/or membrane. ...
Spatial Analysis
The Spatial Analysis module is a suite of tools for analyzing proximity and relative spatial distribution of objects, cells, and/or features across single tissues or serial sections. This module is of particular importance for those conducting immuno-oncology research. ...
Membrane FL
The Membrane FL Quantification module measures fluorescently-labeled membranous markers on a cell-by-cell basis and scores cells according to membrane marker intensity. ...
ISH Quantification
The ISH Quantification module measures one or two chromogen and/or silver-labeled DNA or RNA ISH probes on a per cell basis and is ideally suited for brightfield single or dual probe RNAscope assays....
Multiplex FISH
Indica Labs’ Multiplex FISH module measures up to four DNA or RNA FISH probes on a per cell basis and is ideally suited for Fluorescent RNAscope assays....
Object Colocalization FL
Identify and count multiple object types and measure object colocalization....
Membrane IHC
Quantify membranous immunohistochemical markers on a cell-by-cell basis....
Cytonuclear IHC
Measures single or double stain IHC positivity cell-by-cell in cytoplasm, nucleus, or both....
Cytonuclear FL
Cytonuclear FL measures the immunofluorescent positivity of an unlimited number of fluorescent markers in the nucleus or cytoplasm and reports the number of cells that are positive for each marker, the intensity score for each cell (0, 1+, 2+, 3+), and colocalization of different marker combinations. Optionally, the tool can output the complete table of cell data including individual cell sizes and intensity measurements for each dye in each cellular compartment. In the dual stained mouse pancreas shown above, the...
Break-Apart & Fusion FISH
Automatically detect translocation by counting dual fusion probes and identifying fused pairs....
Amplification & Deletion FISH
Quantitate gene amplification and deletion by counting dual probes and probe ratios....
Serial Registration Analysis
Analyze serially stained tissue sections or a single tissue section which has been stained, stripped and re-stained for multiple markers....
Tissue Classifier
State-of-the-art pattern recognition module for automatic tissue classification and region-of-interest selection...
Tissue Microarray (TMA)
Automated quantification of TMAs in both brightfield and immunofluorescence....